Distinct effect of a cationic surfactant on the transient and steady state phases of 2-naphthyl acetate hydrolysis catalyzed by α-chymotrypsin

Journal of Molecular Catalysis B: Enzymatic

Volumn 31, Issue 4-6, 2004, Pages 83-85

  Abuin, Elsa ^a   Lissi, Eduardo ^a   Duarte, Roxanna ^a  

^a UNIVERSIDAD DE SANTIAGO DE CHILE   (Chile)

Author keywords

Chymotrypsin; 2 Naphthyl acetate; Dodecyltrimethylammonium bromide

Indexed keywords

ACYL-ENZYME INTERMEDIATES; ENZYME-SUBSTRATE COMPLEX; KINETIC PARAMETERS;

ADDITION REACTIONS; CATALYSIS; ENZYMES; HYDROLYSIS; MICELLES; POSITIVE IONS;

SURFACE ACTIVE AGENTS;

2 NAPHTHYL ACETATE; ACETIC ACID DERIVATIVE; CATIONIC SURFACTANT; CHYMOTRYPSIN A; DODECYLTRIMETHYLAMMONIUM BROMIDE; UNCLASSIFIED DRUG;

ADDITION REACTION; ARTICLE; DECOMPOSITION; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME MODIFICATION; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; MICELLE; OBSERVATION; STEADY STATE;

EID: 8644266002     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcatb.2004.07.009     Document Type: Article

References (15)

1. S. Barbaric, and P.L. Luisi J. Am. Chem. Soc. 103 1981 4239
2. N. Spetri, F. Alfani, M. Cantarella, F. D'Amico, E. Germani, and G. Savelli J. Mol. Catal. 6 1999 99
3. P. Viparelli, F. Alfani, and M. Cantarella Biochem. J. 344 1999 765
4. F. Alfani, F.M. Cantarella, N. Spetri, R. Germani, and G. Savelli Appl. Biochem. Biotechnol. 88 2000 1
5. P. Viparelli, F. Alfani, and M. Cantarella J. Mol. Catal. B: Enzym. 15 2001 1
6. Y. Miyake, T. Owari, F. Ishiga, and M. Teramoto J. Chem. Soc. Faraday Trans. 90 1994 979
7. F.M. Menger, and K. Yamada J. Am. Chem. Soc. 101 1979 6371
8. L.F. Aguilar, E. Abuin, and E. Lissi Arch. Biochem. Biophys. 388 2001 231
9. E. Abuin, E. Lissi, and R. Duarte Langmuir 19 2003 5374
10. C.K. Mathews, and K.E. van Holde Biochemistry 1990 Benjamin/Cummings (chapter 10)
11. E. Abuin, E. Lissi, R. Duarte, J. Colloid Interface Sci. (in press).
12. M. Blocher, P. Walde, and I.J. Dunn Biotechnol. Bioeng. 62 1999 36
13. E.A. Belyaeva, D.V. Gra, and N.L. Eremeev Biochemistry (Moscow) 67 2002 1032
14. E. Abuin, and J.C. Scaiano J. Am. Chem. Soc. 106 1984 6274
15. The value differs by a factor 0.55 from that previously reported [9] due to an error in the previous estimation of the enzyme concentration.