Investigation of the substrate specificity of a β-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis

European Journal of Biochemistry

Volumn 271, Issue 21, 2004, Pages 4169-4177

  Marana, Sandro R ^a   Andrade, Eduardo H P ^a   Ferreira, Clélia ^a   Terra, Walter R ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

glycosidase; Bioenergetics analysis; Enzyme specificity; Glycoside hydrolase; Site directed mutagenesis

Indexed keywords

ASPARAGINE; BETA GLYCOSIDASE; GALACTOSIDE; GLUCOSIDE; GLUTAMINE; GLYCOSIDASE; SERINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BIOENERGY; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STRUCTURE; HYDROLYSIS; LEPIDOPTERA; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STEADY STATE; STEREOCHEMISTRY;

ANIMALS; ASPARTIC ACID; BINDING SITES; FUCOSE; GALACTOSIDES; GENE DELETION; GLUCOSIDES; GLUTAMINE; GLYCOSIDE HYDROLASES; HYDROLYSIS; KINETICS; MODELS, CHEMICAL; MODELS, THEORETICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SERINE; SPODOPTERA; SUBSTRATE SPECIFICITY; TEMPERATURE; THERMODYNAMICS; TIME FACTORS;

LEPIDOPTERA; SPODOPTERA; SPODOPTERA FRUGIPERDA;

EID: 8644230008     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.2004.04354.x     Document Type: Article

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