Expansion of human airway basal stem cells and their differentiation as 3D tracheospheres

Methods in Molecular Biology

Volumn 1576, Issue , 2019, Pages 43-53

  Hynds, Robert E ^a   Butler, Colin R ^a   Janes, Sam M ^a   Giangreco, Adam ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Adult stem cells; Cell culture techniques; Cilia; Epithelial cells; Goblet cells; Lung; Primary cell culture; Stem cells

Indexed keywords

AIRWAY EPITHELIUM CELL; CELL CULTURE; CELL DIFFERENTIATION; CELL EXPANSION; CILIATED EPITHELIUM CELL; EPITHELIUM BASAL CELL; FEEDER CELL; FIBROBLAST; GENOMIC INSTABILITY; GOBLET CELL; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; ORGANOID; RESPIRATORY EPITHELIUM; STEM CELL; CELL CULTURE TECHNIQUE; CELL PROLIFERATION; CYTOLOGY; EPITHELIUM CELL; PROCEDURES; TISSUE ENGINEERING; TRACHEA;

CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL PROLIFERATION; CELLS, CULTURED; EPITHELIAL CELLS; HUMANS; ORGANOIDS; STEM CELLS; TISSUE ENGINEERING; TRACHEA;

EID: 85072142592     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/7651_2016_5     Document Type: Chapter

References (16)

1. Hogan BL, Barkauskas CE, Chapman HA, Epstein JA, Jain R, Hsia CC, Niklason L, Calle E, Le A, Randell SH, Rock J, Snitow M, Krummel M, Stripp BR, Vu T, White ES, Whit-sett JA, Morrisey EE (2014) Repair and regeneration of the respiratory system: complexity, plasticity, and mechanisms of lung stem cell function. Cell Stem Cell 15:123–138
2. Rock JR, Onaitis MW, Rawlins EL, Lu Y, Clark CP, Xue Y, Randell SH, Hogan BL (2009) Basal cells as stem cells of the mouse trachea and human airway epithelium. Proc Natl Acad Sci U S A 106:12771–12775
3. Teixeira VH, Nadarajan P, Graham TA, Pipini-kas CP, Brown JM, Falzon M, Nye E, Poulsom R, Lawrence D, Wright NA, McDonald S, Giangreco A, Simons BD, Janes SM (2013) Stochastic homeostasis in human airway epithelium is achieved by neutral competition of basal cell progenitors. Elife 2, e00966
4. Hynds RE, Giangreco A (2013) Concise review: the relevance of human stem cell-derived organoid models for epithelial translational medicine. Stem Cells 31:417–422
5. de Jong PM, van Sterkenburg MA, Hesseling SC, Kempenaar JA, Mulder AA, Mommaas AM, Dijkman JH, Ponec M (1994) Ciliogen-esis in human bronchial epithelial cells cultured at the air-liquid interface. Am J Respir Cell Mol Biol 10:271–277
6. Fulcher ML, Gabriel S, Burns KA, Yankaskas JR, Randell SH (2005) Well-differentiated human airway epithelial cell cultures. Methods Mol Med 107:183–206
7. Mathis C, Poussin C, Weisensee D, Gebel S, Hengstermann A, Sewer A, Belcastro V, Xiang Y, Ansari S, Wagner S, Hoeng J, Peitsch MC (2013) Human bronchial epithelial cells exposed in vitro to cigarette smoke at the air-liquid interface resemble bronchial epithelium from human smokers. Am J Physiol Lung Cell Mol Physiol 304:L489–L503
8. Rheinwald JG, Green H (1975) Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 6:331–343
9. Chapman S, Liu X, Meyers C, Schlegel R, McBride AA (2010) Human keratinocytes are efficiently immortalized by a Rho kinase inhibitor. J Clin Invest 120:2619–2626
10. Suprynowicz FA, Upadhyay G, Krawczyk E, Kramer SC, Hebert JD, Liu X, Yuan H, Che-luvaraju C, Clapp PW, Boucher RC Jr, Kamon-joh CM, Randell SH, Schlegel R (2012) Conditionally reprogrammed cells represent a stem-like state of adult epithelial cells. Proc Natl Acad Sci U S A 109:20035–20040
11. Liu X, Ory V, Chapman S, Yuan H, Albanese C, Kallakury B, Timofeeva OA, Nealon C, Dakic A, Simic V, Haddad BR, Rhim JS, Dritschilo A, Riegel A, McBride A, Schlegel R (2012) ROCK inhibitor and feeder cells induce the conditional reprogramming of epithelial cells. Am J Pathol 180:599–607
12. Butler CR, Hynds RE, Gowers KH, Lee Ddo H, Brown JM, Crowley C, Teixeira VH, Smith CM, Urbani L, Hamilton NJ, Thakrar RM, Booth HL, Birchall MA, De Coppi P, Giangreco A, O’Callaghan C, Janes SM (2016) Rapid Expansion of Human Epithelial Stem Cells Suitable for Airway Tissue Engineering. Am J Respir Crit Care Med194(2):156–168. doi:10.1164/rccm.201507-1414OC, PubMed PMID: 26840431
13. Wu X, Peters-Hall JR, Bose S, Pena MT, Rose MC (2011) Human bronchial epithelial cells differentiate to 3D glandular acini on basement membrane matrix. Am J Respir Cell Mol Biol 44:914–921
14. Hegab AE, Ha VL, Darmawan DO, Gilbert JL, Ooi AT, Attiga YS, Bisht B, Nickerson DW, Gomperts BN (2012) Isolation and in vitro characterization of basal and submucosal gland duct stem/progenitor cells from human proximal airways. Stem Cells Transl Med 1:719–724
15. Danahay H, Pessotti AD, Coote J, Montgomery BE, Xia D, Wilson A, Yang H, Wang Z, Bevan L, Thomas C, Petit S, London A, LeMotte P, Doe-lemeyer A, Velez-Reyes GL, Bernasconi P, Fryer CJ, Edwards M, Capodieci P, Chen A, Hild M, Jaffe AB (2015) Notch2 is required for inflammatory cytokine-driven goblet cell metaplasia in the lung. Cell Rep 10:239–252
16. Gao X, Bali AS, Randell SH, Hogan BL (2015) GRHL2 coordinates regeneration of a polarized mucociliary epithelium from basal stem cells. J Cell Biol 211:669–682