Constitutive expression of human telomerase enhances the proliferation potential of human mesenchymal stem cells

BioResearch Open Access

Volumn 1, Issue 6, 2012, Pages 273-279

  Bischoff, David S ^a,b   Makhijani, Nalini S ^a   Yamaguchi, Dean T ^a,b  

^a VETERANS AFFAIRS MEDICAL CENTER   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

adipogenic differentiation; human mesenchymal stem cell; osteoblastic differentiation; TERT expression

Indexed keywords

FAT DROPLET; MESSENGER RNA; TELOMERASE REVERSE TRANSCRIPTASE;

ADULT; APOPTOSIS; ARTICLE; CASE REPORT; CELL DIFFERENTIATION; CELL GROWTH; CELL PROLIFERATION; HUMAN; HUMAN CELL; IN VITRO STUDY; LENTIVIRINAE; MALE; MESENCHYMAL STEM CELL; PRIORITY JOURNAL; SENESCENCE;

LENTIVIRUS;

EID: 85053892677     PISSN: None     EISSN: 21647860     Source Type: Journal    
DOI: 10.1089/biores.2012.0252     Document Type: Article

References (24)

1. Caplan AI. Mesenchymal stem cells. J Orthop Res. 1991; 9:641-650.
2. Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284:143-147.
3. Bianco P, Robey PG, Simmons PJ. Mesenchymal stem cells: revisiting history, concepts, and assays. Cell Stem Cell. 2008;2:313-319.
4. Stenderup K, Justesen J, Clausen C, et al. Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells. Bone. 2003;33:919-926.
5. Metz T, Verdonk G. In vitro aging of human bone marrow derived stromal cells. Mech Ageing Dev. 1981;16:81-89.
6. Bruder SP, Jaiswal N, Haynesworth SE. Growth kinetics, selfrenewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. J Cell Biochem. 1997;64: 278-294.
7. Nicholls C, Li H, Wang JQ, et al. Molecular regulation of telomerase activity in aging. Protein Cell. 2011;2:726-738.
8. Ayouaz A, Raynaud C, Heride C, et al. Telomeres: hallmarks of radiosensitivity. Biochimie. 2008;90:60-72.
9. Rochette PJ, Brash DE. Human telomeres are hypersensitive to UV-induced DNA Damage and refractory to repair. PLoS Genet. 2010;6:e1000926.
10. de Lange T. How telomeres solve the end-protection problem. Science. 2009;326:948-952.
11. Greider CW, Blackburn EH. The telomere terminal transferase of Tetrahymena is a ribonucleoprotein enzyme with two kinds of primer specificity. Cell. 1987;51:887-898.
12. Shay JW, Bacchetti S. A survey of telomerase activity in human cancer. Eur J Cancer. 1997;33:787-791.
13. Toma's-Loba A, Flores I, Ferna'ndez-Marcos PJ, et al. Telomerase reverse transcriptase delays aging in cancer-resistant mice. Cell. 2008;135:609-622.
14. Bo?kerW, Yin Z,Drosse I, et al. Introducing a single-cell-derived human mesenchymal stem cell line expressing hTERT after lentiviral gene transfer. J Cell Mol Med. 2008;12:1347-1359.
15. Nakahara H, Misawa H, Hayashi T, et al. Bone repair by transplantation of hTERT-immortalized human mesenchymal stem cells in mice. Transplantation. 2009;88:346-353.
16. Simonsen JL, Rosada C, Serakinci N, et al. Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells. Nat Biotechnol. 2002;20:592-596.
17. Counter CM, Hahn WC, Wei W, et al. Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization. Proc Natl Acad Sci USA. 1998;95:14723- 14728.
18. Andley UP, Rhim JS, Chylack LT Jr, et al. Propagation and immortalization of human lens epithelial cells in culture. Invest Ophthalmol Vis Sci. 1994;35:3094-3102.
19. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 1(-DDC(T)) method. Methods. 2001;25:402-408.
20. Makhijani NS, Bischoff DS, Yamaguchi DT. Regulation of proliferation and migration in retinoic acid treated C3H10T1/2 cells by TGF-b isoforms. J. Cell Physiol. 2005;202:304-313.
21. Bischoff DS, Zhu JH, Makhijani NS, et al. Angiogenic CXC chemokine expression during differentiation of human mesenchymal stem cells towards the osteoblastic lineage. J Cell Biochem. 2008;103:812-824.
22. Harley CB. Telomerase is not an oncogene. Oncogene. 2002; 21:494-502.
23. Kuro-O M. Klotho and aging. Biochim Biophys Acta. 2009; 1790:1049-1058.
24. Krishnamurthy J, Ramsey MR, Ligon KL, et al. p16INK4a induces an age-dependent decline in islet regenerative potential. Nature. 2006;443:453-457.