Quantification of GRAF gene expression in patients with acute myeloid leukemia using EvaGreen real time quantitative PCR

Chinese Journal of Medical Genetics

Volumn 27, Issue 3, 2010, Pages 290-293

  Qian, Zhen ^a   Lin, Jiang ^a   Qian, Jun ^a   Yao, Dong Ming ^a   Wang, Ya Li ^a   Han, Lan Xiu ^a   Zhu, Zhao Hui ^a   Xiao, Gao Fei ^a  

^a JIANGSU UNIVERSITY   (China)

Author keywords

Acute myeloid leukemia; EvaGreen; GTPase regulator associated with the focal adhesion kinase; Real time quantitative PCR

Indexed keywords

GTPASE REGULATOR ASSOCIATED WITH THE FOCAL ADHESION KINASE; TUMOR PROTEIN; UNCLASSIFIED DRUG; ARHGAP26 PROTEIN, HUMAN; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN;

ACUTE GRANULOCYTIC LEUKEMIA; ARTICLE; CANCER PATIENT; CONTROLLED STUDY; DOWN REGULATION; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENETIC TRANSCRIPTION; HEMATOLOGIC DISEASE; HUMAN; LEUKEMOGENESIS; MAJOR CLINICAL STUDY; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TUMOR GENE; ADOLESCENT; ADULT; AGED; CHILD; FEMALE; GENETICS; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD; PROCEDURES; VERY ELDERLY; YOUNG ADULT;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; CHILD; CHILD, PRESCHOOL; FEMALE; GTPASE-ACTIVATING PROTEINS; HUMANS; LEUKEMIA, MYELOID, ACUTE; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; YOUNG ADULT;

EID: 85052488833     PISSN: 10039406     EISSN: None     Source Type: Journal    
DOI: 10.3760/cma.j.issn.1003-9406.2010.03.012     Document Type: Article

References (9)

1. Hildebrand JD, Taylor JM, Parsons JT, et al. An SH3 domain-containing GTPase-activating protein for Rho and Cdc42 associates with focal adhesion kinase. Mol Cell Biol, 1996, 6: 3169-3178.
2. Borkhardt A, Bojesen S, Haas OA, et al. The human GRAF gene is fused to MLL in a unique t(5;11) (q31;q23) and both alleles are disrupted in three cases of myelodysplastic syndrome/acute myeloid leukemia with a deletion 5q. Proc Natl Acad Sci U S A, 2000, 97: 9168-9173.
3. Zhang ZN, Shen T. The standards of diagnostic and therapeutic effect of hematologic disease. 3rd. Beijing: Science Publish House, 2007. 106-116.
4. Slovak ML, Kopecky KJ, Cassileth PA, et al. Karyotypic analysis predicts outcome of preremission and postremission therapy in adult acute myeloid leukemia: a Southwest Oncology Group/Eastern Cooperative Oncology Group Study. Blood, 2000, 96: 4075-4083.
5. Qiu RG, Chen J, Mccormick F, et al. Role for Rho in Ras transformation. Proc Natl Acad Sci U S A, 1995, 92: 11781-11785.
6. Olson MF, Paterson HF, Marshall CJ. Signals from Ras and Rho GTPases interact to regulate expression of p21Waf1/Cip1. Nature, 1998, 394: 295-299.
7. Panagopoulos I, Kitagawa A, Isaksson M, et al. MLL/GRAF fusion in an infant acute monocytic leukemia (AML M5b) with a cytogenetically cryptic ins (5;11) (q31;q23q23). Genes Chromosomes Cancer, 2004, 41: 400-404.
8. Bojesen S, Ammerpohl O, Weinhausl A, et al. Characterization of the GRAF gene promoter and its methylation in patients with acute myeloid leukaemia and myelodysplastic syndrome. Br J Cancer, 2006, 94: 323-332.
9. Mao F, Leung WY, Xin X. Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications. BMC Biotechnol, 2007, 7: 76.