Quantitative recovery of viable Lactobacillus paracasei CNCM I-1572 (L. casei DG®) after gastrointestinal passage in healthy adults

Frontiers in Microbiology

Volumn 9, Issue AUG, 2018, Pages

  Arioli, Stefania ^a   Koirala, Ranjan ^a   Taverniti, Valentina ^a   Fiore, Walter ^a,b   Guglielmetti, Simone ^a  

^a UNIVERSITY OF MILAN   (Italy)

^b ALF X Surgical Robotics Department   (Italy)

Author keywords

Enterolactis; EPS; Isolation; Probiotic; QPCR

Indexed keywords

ENTEROLACTIS; KANAMYCIN; PROBIOTIC AGENT; REVENANT DG; VANCOMYCIN;

ADULT; ARTICLE; BACTERIAL LOAD; BACTERIAL STRAIN; COLONY FORMING UNIT; CONTROLLED STUDY; CORRELATION ANALYSIS; DEFECATION HABIT; DNA EXTRACTION; ENTEROLACTIS; FECES ANALYSIS; FEMALE; FOLLOW UP; GASTROINTESTINAL TRANSIT; HUMAN; LACTOBACILLUS PARACASEI; MALE; MICROBIAL SURVIVAL; NONHUMAN; NORMAL HUMAN; OBSERVATIONAL STUDY; OPEN STUDY; PILOT STUDY; REAL TIME POLYMERASE CHAIN REACTION; SURVIVAL ANALYSIS; VIABLE CELL COUNT;

EID: 85051077506     PISSN: None     EISSN: 1664302X     Source Type: Journal    
DOI: 10.3389/fmicb.2018.01720     Document Type: Article

References (34)

1. Ahlroos, T., and Tynkkynen, S. (2009). Quantitative strain-specific detection of Lactobacillus rhamnosus GG in human faecal samples by real-time PCR. J. Appl. Microbiol. 106, 506-514. doi: 10.1111/j.1365-2672.2008.04018.x
2. Balzaretti, S., Taverniti, V., Guglielmetti, S., Fiore, W., Minuzzo, M., Ngo, H. N., et al. (2017). A novel rhamnose-rich hetero-exopolysaccharide isolated from Lactobacillus paracasei DG activates THP-1 human monocytic cells. Appl. Environ. Microbiol. 83:e02702-16. doi: 10.1128/AEM.02702-16
3. Balzaretti, S., Taverniti, V., Rondini, G., Marcolegio, G., Minuzzo, M., Remagni, M. C., et al. (2015). The vaginal isolate Lactobacillus paracasei LPC-S01 (DSM 26760) is suitable for oral administration. Front. Microbiol. 6:952. doi: 10.3389/fmicb.2015.00952
4. Borchers, A. T., Selmi, C., Meyers, F. J., Keen, C. L., and Gershwin, M. E. (2009). Probiotics and immunity. J. Gastroenterol. 44, 26-46. doi: 10.1007/s00535-008-2296-0
5. Compare, D., Rocco, A., Coccoli, P., Angrisani, D., Sgamato, C., Iovine, B., et al. (2017). Lactobacillus casei DG and its postbiotic reduce the inflammatory mucosal response: an ex-vivo organ culture model of post-infectious irritable bowel syndrome. BMC Gastroenterol. 17:53. doi: 10.1186/s12876-017-0605-x
6. Cremon, C., Guglielmetti, S., Gargari, G., Taverniti, V., Castellazzi, A. M., Valsecchi, C., et al. (2017). Effect of Lactobacillus paracasei CNCM I-1572 on symptoms, gut microbiota, short chain fatty acids, and immune activation in patients with irritable bowel syndrome: a pilot randomized clinical trial. United Eur. Gastroenterol. J. 6, 604-613. doi: 10.1177/2050640617736478
7. da Cruz, A. G., Faria, J. D. A. F., and Van Dender, A. G. F. (2007). Packaging system and probiotic dairy foods. Food Res. Int. 40, 951-956. doi: 10.1016/j.foodres.2007.05.003
8. Derrien, M., and van Hylckama Vlieg, J. E. (2015). Fate, activity, and impact of ingested bacteria within the human gut microbiota. Trends Microbiol. 23, 354-366. doi: 10.1016/j.tim.2015.03.002
9. D'Incà, R., Barollo, M., Scarpa, M., Grillo, A. R., Brun, P., Vettorato, M. G., et al. (2011). Rectal administration of Lactobacillus casei DG modifies flora composition and toll-like receptor expression in colonic mucosa of patients with mild ulcerative colitis. Dig. Dis. Sci. 56, 1178-1187. doi: 10.1007/s10620-010-1384-1
10. Dommels, Y. E., Kemperman, R. A., Zebregs, Y. E., Draaisma, R. B., Jol, A., Wolvers, D. A., et al. (2009). Survival of Lactobacillus reuteri DSM 17938 and Lactobacillus rhamnosus GG in the human gastrointestinal tract with daily consumption of a low-fat probiotic spread. Appl. Environ. Microbiol. 75, 6198-6204. doi: 10.1128/AEM.01054-09
11. European Food Safety Authority [EFSA] (2012). Outcome of the public consultation on the draft guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance. EFSA Support. Publ. 9:EN-316
12. FAO/WHO (2001). Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. Córdoba: Argentina
13. Ferrario, C., Taverniti, V., Milani, C., Fiore, W., Laureati, M., De Noni, I., et al. (2014). Modulation of fecal Clostridiales bacteria and butyrate by probiotic intervention with Lactobacillus paracasei DG varies among healthy adults. J. Nutr. 144, 1787-1796. doi: 10.3945/jn.114.197723
14. Goderska, K. (2012). "Different Methods of Probiotics Stabilization, "in Probiotics, ed. P. E. Rigobelo (Rijeka: InTech)
15. Granata, M., Brandi, G., Borsari, A., Gasbarri, R., and Gioia, D. D. (2013). Synbiotic yogurt consumption by healthy adults and the elderly: the fate of bifidobacteria and LGG probiotic strain. Int. J. Food Sci. Nutr. 64, 162-168. doi: 10.3109/09637486.2012.718742
16. Hamilton-Miller, J. M., Shah, S., and Winkler, J. T. (1999). Public health issues arising from microbiological and labelling quality of foods and supplements containing probiotic microorganisms. Public Health Nutr. 2, 223-229. doi: 10.1017/S1368980099000282
17. Hill, C., Guarner, F., Reid, G., Gibson, G. R., Merenstein, D. J., Pot, B., et al. (2014). Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat. Rev. Gastroenterol. Hepatol. 11, 506-514. doi: 10.1038/nrgastro.2014.66
18. Lahtinen, S. J. (2012). Probiotic viability-does it matter? Microb. Ecol. Health Dis. 23. doi: 10.3402/mehd.v23i0.18567
19. Larsen, C. N., Nielsen, S., Kaestel, P., Brockmann, E., Bennedsen, M., Christensen, H. R., et al. (2006). Dose-response study of probiotic bacteria Bifidobacterium animalis subsp lactis BB-12 and Lactobacillus paracasei subsp paracasei CRL-341 in healthy young adults. Eur. J. Clin. Nutr. 60, 1284-1293. doi: 10.1038/sj.ejcn.1602450
20. Mai, V., Waugh, S., Byrd, D., Simpson, D., and Ukhanova, M. (2017). Novel encapsulation improves recovery of probiotic strains in fecal samples of human volunteers. Appl. Microbiol. Biotechnol. 101, 1419-1425. doi: 10.1007/s00253-016-7915-8
21. Pino, A., Van Hoorde, K., Pitino, I., Russo, N., Carpino, S., Caggia, C., et al. (2017). Survival of potential probiotic Lactobacilli used as adjunct cultures on pecorino siciliano cheese ripening and passage through the gastrointestinal tract of healthy volunteers. Int. J. Food Microbiol. 252, 42-52. doi: 10.1016/j.ijfoodmicro.2017.04.012
22. Poutsiaka, D. D., Mahoney, I. J., Mcdermott, L. A., Stern, L. L., Thorpe, C. M., Kane, A. V., et al. (2017). Selective method for identification and quantification of Bifidobacterium animalis subspecies lactis BB-12 (BB-12) from the gastrointestinal tract of healthy volunteers ingesting a combination probiotic of BB-12 and Lactobacillus rhamnosus GG. J. Appl. Microbiol. 122, 1321-1332. doi: 10.1111/jam.13436
23. Prilassnig, M., Wenisch, C., Daxboeck, F., and Feierl, G. (2007). Are probiotics detectable in human feces after oral uptake by healthy volunteers? Wien. Klin. Wochenschr. 119, 456-462. doi: 10.1007/s00508-007-0808-1
24. Rosania, R., Giorgio, F., Principi, M., Amoruso, A., Monno, R., Di Leo, A., et al. (2013). Effect of probiotic or prebiotic supplementation on antibiotic therapy in the small intestinal bacterial overgrowth: a comparative evaluation. Curr. Clin. Pharmacol. 8, 169-172. doi: 10.2174/15748847113089990048
25. Savini, M., Cecchini, C., Verdenelli, M. C., Silvi, S., Orpianesi, C., and Cresci, A. (2010). Pilot-scale production and viability analysis of freeze-dried probiotic bacteria using different protective agents. Nutrients 2, 330-339. doi: 10.3390/nu2030330
26. Saxelin, M., Lassig, A., Karjalainen, H., Tynkkynen, S., Surakka, A., Vapaatalo, H., et al. (2010). Persistence of probiotic strains in the gastrointestinal tract when administered as capsules, yoghurt, or cheese. Int. J. Food Microbiol. 144, 293-300. doi: 10.1016/j.ijfoodmicro.2010.10.009
27. Songisepp, E., Kals, J., Kullisaar, T., Mandar, R., Hutt, P., Zilmer, M., et al. (2005). Evaluation of the functional efficacy of an antioxidative probiotic in healthy volunteers. Nutr. J. 4:22. doi: 10.1186/1475-2891-4-22
28. Temmerman, R., Pot, B., Huys, G., and Swings, J. (2003). Identification and antibiotic susceptibility of bacterial isolates from probiotic products. Int. J. Food Microbiol. 81, 1-10. doi: 10.1016/S0168-1605(02)00162-9
29. Tuohy, K. M., Pinart-Gilberga, M., Jones, M., Hoyles, L., Mccartney, A. L., and Gibson, G. R. (2007). Survivability of a probiotic Lactobacillus casei in the gastrointestinal tract of healthy human volunteers and its impact on the faecal microflora. J. Appl. Microbiol. 102, 1026-1032
30. Turco, F., Andreozzi, P., Palumbo, I., Zito, F. P., Cargiolli, M., Fiore, W., et al. (2017). Bacterial stimuli activate nitric oxide colonic mucosal production in diverticular disease. Protective effects of L. casei DG® (Lactobacillus paracasei CNCM I-1572). United Eur. Gastroenterol. J. 5, 715-724. doi: 10.1177/2050640616684398
31. Tursi, A., Brandimarte, G., Elisei, W., Picchio, M., Forti, G., Pianese, G., et al. (2013). Randomised clinical trial: mesalazine and/or probiotics in maintaining remission of symptomatic uncomplicated diverticular disease-a double-blind, randomised, placebo-controlled study. Aliment. Pharmacol. Ther. 38, 741-751. doi: 10.1111/apt.12463
32. Verdenelli, M. C., Ghelfi, F., Silvi, S., Orpianesi, C., Cecchini, C., and Cresci, A. (2009). Probiotic properties of Lactobacillus rhamnosus and Lactobacillus paracasei isolated from human faeces. Eur. J. Nutr. 48, 355-363. doi: 10.1007/s00394-009-0021-2
33. Verna, E. C., and Lucak, S. (2010). Use of probiotics in gastrointestinal disorders: what to recommend? Therap. Adv. Gastroenterol. 3, 307-319. doi: 10.1177/1756283X10373814
34. Wang, R., Chen, S., Jin, J., Ren, F., Li, Y., Qiao, Z., et al. (2015). Survival of Lactobacillus casei strain Shirota in the intestines of healthy Chinese adults. Microbiol. Immunol. 59, 268-276. doi: 10.1111/1348-0421.12249