Knockout mutation of p27-p55 operon severely reduces replication of mycobacterium bovis in a macrophagic cell line and survival in a mouse model of infection

Virulence

Volumn 2, Issue 3, 2011, Pages 233-237

  Bianco, María Verónica ^a   Blanco, Federico Carlos ^a   Forrellad, Marina Andrea ^a   Aguilar, Diana ^b   Eleonora, Campos ^a   Klepp, Laura Inés ^a   Hernández Pando, Rogelio ^b   Cataldi, Angel Adrian ^a   Bigi, Fabiana ^a  

^a INSTITUTO NACIONAL DE TECNOLOGÍA AGROPECUARIA INTA   (Argentina)

^b INSTITUTO NACIONAL DE CIENCIAS MÉDICAS Y NUTRICIÓN SALVADOR ZUBIRÁN   (Mexico)

Author keywords

LprG; Mycobacterium tuberculosis; Virulence

Indexed keywords

GLYCOLIPID; LIPOPROTEIN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2; BACTERIAL PROTEIN; CARRIER PROTEIN; P27 PROTEIN, MYCOBACTERIUM; P55 PROTEIN, MYCOBACTERIUM; VIRULENCE FACTOR;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIGEN PRESENTATION; ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BOVINE TUBERCULOSIS; GENE; GENE MUTATION; GENETIC COMPLEMENTATION; IMMUNE RESPONSE; IMMUNOMODULATION; LUNG TUBERCULOSIS; MACROPHAGE; MOUSE; MYCOBACTERIUM BOVIS; NONHUMAN; OPERON; P27 GENE; P55 GENE; PROTEIN LIPID INTERACTION; SURVIVAL; ANIMAL; BACTERIAL COUNT; BAGG ALBINO MOUSE; CATTLE; CELL LINE; DISEASE MODEL; GENE DELETION; GENETICS; LUNG; MICROBIAL VIABILITY; MICROBIOLOGY; PATHOGENICITY; PATHOLOGY; VIRULENCE;

BOVINAE; MURINAE; MUS; MYCOBACTERIUM; MYCOBACTERIUM BOVIS; MYCOBACTERIUM TUBERCULOSIS;

ANIMALS; BACTERIAL PROTEINS; CATTLE; CELL LINE; COLONY COUNT, MICROBIAL; DISEASE MODELS, ANIMAL; GENE DELETION; GENETIC COMPLEMENTATION TEST; LIPOPROTEINS; LUNG; MACROPHAGES; MEMBRANE TRANSPORT PROTEINS; MICE; MICE, INBRED BALB C; MICROBIAL VIABILITY; MYCOBACTERIUM BOVIS; OPERON; TUBERCULOSIS, BOVINE; TUBERCULOSIS, PULMONARY; VIRULENCE; VIRULENCE FACTORS;

EID: 85046979825     PISSN: 21505594     EISSN: 21505608     Source Type: Journal    
DOI: 10.4161/viru.2.3.15888     Document Type: Article

References (20)

1. Bigi F, Alito A, Romano MI, Zumarraga M, Caimi K, Cataldi A. The gene encoding P27 lipoprotein and a putative antibiotic-resistance gene form an operon in Mycobacterium tuberculosis and Mycobacterium bovis. Microbiology 2000; 146:1011-1018.
2. Bigi F, Espitia C, Alito A, Zumarraga M, Romano MI, Cravero S, et al. A novel 27 kDa lipoprotein antigen from Mycobacterium bovis. Microbiology 1997; 143:3599-3605.
3. Hovav AH, Mullerad J, Davidovitch L, Fishman Y, Bigi F, Cataldi A, et al. The Mycobacterium tuberculosis recombinant 27-kilodalton lipoprotein induces a strong Th1-type immune response deleterious to protection. Infect Immun 2003; 71:3146-3154.
4. González-Zamorano M, Mendoza-Hernández G, Xolalpa W, Parada C, Vallecillo AJ, Bigi F, et al. Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins. J Proteome Res 2009; 8:721-733.
5. Drage MG, Tsai HC, Pecora ND, Cheng TY, Arida AR, Shukla S, et al. Mycobacterium tuberculosis lipoprotein LprG Rv1411c binds triacylated glycolipid agonists of Toll-like receptor 2. Nat Struct Mol Biol 2010; 17:1088-1095.
6. Silva PE, Bigi F, Santangelo MP, Romano MI, Martín C, Cataldi A, et al. Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis. Antimicrob Agents Chemother 2001; 45:800-804.
7. Farrow MF, Rubin EJ. Function of a mycobacterial major facilitator superfamily pump requires a membrane-associated lipoprotein. J Bacteriol 2008; 190:1783-1791.
8. Jiang X, Zhang W, Zhang Y, Gao F, Lu C, Zhang X, et al. Assessment of efflux pump gene expression in a clinical isolate Mycobacterium tuberculosis by real-time reverse transcription PCR. Microb Drug Resist 2008; 14:7-11.
9. Ramón-García S, Martín C, Thompson CJ, Aínsa JA. Role of the Mycobacterium tuberculosis P55 efflux pump in intrinsic drug resistance, oxidative stress responses, and growth. Antimicrob Agents Chemother 2009; 53:3675-3682.
10. Bigi F, Gioffré A, Klepp L, Santangelo MP, Alito A, Caimi K, et al. The knockout of the lprG-Rv1410 operon produces strong attenuation of Mycobacterium tuberculosis. Microbes Infect 2004; 6:182-187.
11. Sieling PA, Hill PJ, Dobos KM, Brookman K, Kuhlman AM, Fabri M, et al. Conserved mycobacterial lipoglycoproteins activate TLR2 but also require glycosylation for MHC class II-restricted T cell activation. J Immunol 2008; 180:5833-5842.
12. Arraiano CM, Andrade JM, Domingues S, Guinote IB, Malecki M, Matos RG, et al. The critical role of RNA processing and degradation in the control of gene expression. FEMS Microbiol Rev 2010: 34:883-923.
13. Gioffre A, Infante E, Aguilar D, Santangelo MP, Klepp L, Amadio A, et al. Mutation in mce operons attenuates Mycobacterium tuberculosis virulence. Microbes Infect 2005: 7:325-334.
14. Parish T, Stoker NG. Use of a flexible cassette method to generate a double unmarked Mycobacterium tuberculosis tlyA plcABC mutant by gene replacement. Microbiology 2000; 146: 969-975.
15. Le Dantec C, Winter N, Gicquel B, Vincent V, Picardeau M. Genomic sequence and transcriptional analysis of a 23-kilobase mycobacterial linear plasmid: evidence for horizontal transfer and identification of plasmid maintenance systems. J Bacteriol 2001; 183:2157-2164.
16. Santangelo MP, Blanco FC, Bianco MV, Klepp LI, Zabal O, Cataldi AA, et al. Study of the role of Mce3R on the transcription of mce genes of Mycobacterium tuberculosis. BMC Microbiol 2008; 8:e38.
17. Dubnau E, Fontán P, Manganelli R, Soares-Appel S, Smith I. Mycobacterium tuberculosis genes induced during infection of human macrophages. Infect Immun 2002; 70:2787-2795.
18. Pfaffl MW, Horgan GW, Dempfle L. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 2002; 30:e36.
19. Ramakers C, Ruijter JM, Deprez RH, Moorman AF. Assumption-free analysis of quantitative real-time PCR data. Neurosci Lett 2003; 13:62-66.
20. Blanco FC, Nunez-Garcia J, García-Pelayo C, Soria M, Bianco MV, Zumárraga M, et al. Differential transcrip-tome profiles of attenuated and hypervirulent strains of Mycobacterium bovis. Microbes Infect 2009; 11:956-963.