ELISPOT-based “Multi-Color FluoroSpot” to study type-specific and cross-reactive responses in memory B cells after dengue and zika virus infections

Methods in Molecular Biology

Volumn 1808, Issue , 2018, Pages 151-163

  Andrade, Paulina ^a,b   Coloma, Josefina ^a   Harris, Eva ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSIDAD SAN FRANCISCO DE QUITO   (Ecuador)

Author keywords

Dengue; Human memory B cells; Multi color FluoroSpot; Peripheral blood mononuclear cells; Qdots; Type specific versus cross reactive responses; Zika

Indexed keywords

QUANTUM DOT; VIRUS ANTIBODY;

ASSAY; CROSS REACTION; DENGUE; ENZYME LINKED IMMUNOSPOT ASSAY; HUMAN; LYMPHOCYTE SUBPOPULATION; MEMORY CELL; MULTI COLOR FLUOROSPOT ASSAY; NICARAGUA; NONHUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; SEROTYPE; ZIKA FEVER; ZIKA VIRUS; ANIMAL; B LYMPHOCYTE; DENGUE VIRUS; HOST PATHOGEN INTERACTION; IMMUNOLOGICAL MEMORY; IMMUNOLOGY; METABOLISM; MONONUCLEAR CELL; PROCEDURES; VIROLOGY;

ANIMALS; ANTIBODIES, VIRAL; B-LYMPHOCYTES; CROSS REACTIONS; DENGUE; DENGUE VIRUS; ENZYME-LINKED IMMUNOSPOT ASSAY; HOST-PATHOGEN INTERACTIONS; HUMANS; IMMUNOLOGIC MEMORY; LEUKOCYTES, MONONUCLEAR; ZIKA VIRUS; ZIKA VIRUS INFECTION;

EID: 85044448375     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-8567-8_13     Document Type: Chapter

References (12)

1. Bhatt S, Gething PW, Brady OJ et al (2013) The global distribution and burden of dengue. Nature 496(7446):504
2. Faria NR, da Silva Azevedo RDS, Kraemer MU et al (2016) Zika virus in the Americas: early epidemiological and genetic findings. Science 352(6283):345–349
3. Oehler E, Watrin L, Larre P et al (2014) Zika virus infection complicated by Guillain-Barre syndrome—case report, French Polynesia, December 2013. Euro Surveill:19(9)
4. PAHO, Regional Zika Epidemiological Update (Americas), 25 May 2017. Available at http://www.paho.org/hq/index.php?option=com_con tent&id=11599&Itemid=41691
5. Ahmed R, Gray D (1996) Immunological memory and protective immunity: understanding their relation. Science 272(5258):54–59
6. Radbruch A, Muehlinghaus G, Luger EO et al (2006) Competence and competition: the challenge of becoming a long-lived plasma cell. Nat Rev Immunol 6(10):741–750
7. Guzman MG, Alvarez M, Rodriguez-Roche R et al (2007) Neutralizing antibodies after infec tion with dengue 1 virus. Emerg Infect Dis 13(2):282
8. Halstead SB (1990) Dengue and dengue hemorrhagic fever. Curr Opin Infect Dis 3(3):434–438
9. Sangkawibha N, Rojanasuphot S, Ahandrik S et al (1984) Risk factors in dengue shock syndrome: a prospective epidemiologic study in Rayong, Thailand: I. The 1980 outbreak. Am J Epidemiol 120(5):653–669
10. Dejnirattisai W, Supasa P, Wongwiwat W et al (2016) Dengue virus sero-cross-reactivity drives antibody-dependent enhancement of infection with Zika virus. Nat Immunol 17(9):1102–1108
11. Hadjilaou A, Green AM, Coloma J, Harris E (2015) Single-cell analysis of B cell/ antibody cross-reactivity using a novel multicolor FluoroSpot assay. J Immunol 195(7):3490–3496
12. Pinna D, Corti D, Jarrossay D et al (2009) Clonal dissection of the human memory B-cell repertoire following infection and vaccination. Eur J Immunol 39(5):1260–1270