Two subunits of the exocyst, Sec3p and Exo70p, can function exclusively on the plasma membrane

Molecular Biology of the Cell

Volumn 29, Issue 6, 2018, Pages 736-750

  Liu, Dongmei ^a   Li, Xia ^a   Shen, David ^a   Novick, Peter ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EXO70P PROTEIN; EXOCYST; POLYLYSINE; PROTEIN; SEC3P PROTEIN; UNCLASSIFIED DRUG; EXO70 PROTEIN, S CEREVISIAE; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE; SACCHAROMYCES CEREVISIAE PROTEIN; SEC3 PROTEIN, S CEREVISIAE; VESICULAR TRANSPORT PROTEIN;

ACTIN FILAMENT; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL FRACTIONATION; CELL LYSATE; CELL MEMBRANE; CELL POLARITY; CELL SURFACE; CELL VIABILITY; CONTROLLED STUDY; ENDOCYTOSIS; GENE LOCUS; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN FUNCTION; SECRETORY VESICLE; STOICHIOMETRY; STRUCTURAL GENE; AMINO ACID SEQUENCE; CYTOPLASM; EXOCYTOSIS; METABOLISM; SACCHAROMYCES CEREVISIAE; ULTRASTRUCTURE;

AMINO ACID SEQUENCE; CELL MEMBRANE; CYTOPLASM; EXOCYTOSIS; PHOSPHATIDYLINOSITOL 4,5-DIPHOSPHATE; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SECRETORY VESICLES; VESICULAR TRANSPORT PROTEINS;

EID: 85043606068     PISSN: 10591524     EISSN: 19394586     Source Type: Journal    
DOI: 10.1091/mbc.E17-08-0518     Document Type: Article

References (44)

1. Aalto MK, Ronne H, Keranen S. (1993). Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport. EMBO J 12, 4095-4104.
2. Abeliovich H, Grote E, Novick P, Ferro-Novick S (1998). Tlg2p, a yeast syntaxin homolog that resides on the Golgi and endocytic structures. J Biol Chem 273, 11719-11727.
3. Apel AR, Hoban K, Chuartzman S, Tonikian R, Sidhu S, Schuldiner M, Wendland B, Prosser D. (2017). Syp1 regulates the clathrin-mediated and clathrin-independent endocytosis of multiple cargo proteins through a novel sorting motif. Mol Biol Cell 28, 2434-2448.
4. Bowser R, Muller H, Govindan B, Novick P. (1992). Sec8p and Sec15p are components of a plasma membrane-associated 19.5S particle that may function downstream of Sec4p to control exocytosis. J Cell Biol 118, 1041-1056.
5. Boyd C, Hughes T, Pypaert M, Novick P. (2004). Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p. J Cell Biol 167, 889-901.
6. Brennwald P, Kearns B, Champion K, Keranen S, Bankaitis V, Novick P. (1994). Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis. Cell 79, 245-258.
7. Brown FC, Pfeffer S.R. (2010). An update on transport vesicle tethering. Mol Membr Biol 27, 457-461.
8. Dong G, Hutagalung AH, Fu C, Novick P, Reinisch K.M. (2005). The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif. Nat Struct Mol Biol 12, 1094-1100.
9. Drees BL, Sundin B, Brazeau E, Caviston J P, Chen GC, Guo W, Kozminski KG, Lau MW, Moskow JJ, Tong A, et al (2001). A protein interaction map of cell polarity development. J Cell Biol 154, 549-571.
10. Dubuke ML, Maniatis S, Shaffer SA, Munson M. (2015). The exocyst subunit Sec6 interacts with assembled exocytic SNARE complexes. J Biol Chem 290, 28245-28256.
11. Finger FP, Hughes TE, Novick P. (1998). Sec3p is a spatial landmark of polarized secretion in budding yeast. Cell 92, 559-571.
12. Finger FP, Novick P. (1997). Sec3p is involved in secretion and morphogenesis in Saccharomyces cerevisiae. Mol Biol Cell 8, 647-662.
13. Gandhi M, Goode BL, Chan C.S. (2006). Four novel suppressors of gic1 gic2 and their roles in cytokinesis and polarized cell growth in Saccharomyces cerevisiae. Genetics 174, 665-678.
14. Guo W, Roth D, Walch-Solimena C, Novick P. (1999). The exocyst is an effector of Sec4p, targeting secretory vesicles to sites of exocytosis. EMBO J 18, 1071-1080.
15. Guo W, Tamanoi F, Novick P. (2001). Spatial regulation of the exocyst complex by Rho1 GTPase. Nat Cell Biol 3, 353-360.
16. Harsay E, Bretscher A. (1995). Parallel secretory pathways to the cell surface in yeast. J Cell Biol 131, 297-310.
17. He B, Xi F, Zhang X, Zhang J, Guo W. (2007). Exo70 interacts with phospholipids and mediates the targeting of the exocyst to the plasma membrane. EMBO J 26, 4053-4065.
18. Heider MR, Gu M, Duffy CM, Mirza AM, Marcotte LL, Walls AC, Farrall N, Hakhverdyan Z, Field MC, Rout MP, et al (2016). Subunit connectivity, assembly determinants and architecture of the yeast exocyst complex. Nat Struct Mol Biol 23, 59-66.
19. Kozminski KG, Alfaro G, Dighe S, Beh C.T. (2006). Homologues of oxysterol-binding proteins affect Cdc42p- and Rho1p-mediated cell polarization in Saccharomyces cerevisiae. Traffic 7, 1224-1242.
20. Kushnirov VV (2000). Rapid and reliable protein extraction from yeast. Yeast 16, 857-860.
21. Lewis MJ, Nichols BJ, Prescianotto-Baschong C, Riezman H, Pelham H.R. (2000). Specific retrieval of the exocytic SNARE Snc1p from early yeast endosomes. Mol Biol Cell 11, 23-38.
22. Liu D, Novick P. (2014). Bem1p contributes to secretory pathway polarization through a direct interaction with Exo70p. J Cell Biol 207, 59-72.
23. Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR (1998). Additional modules of versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14, 953-961.
24. McNew JA, Parlati F, Fukuda R, Johnston RJ, Paz K, Paumet F, Sollner TH, Rothman J.E. (2000). Compartmental specificity of cellular membrane fusion encoded in SNARE proteins. Nature 407, 153-159.
25. Morgera F, Sallah MR, Dubuke ML, Gandhi P, Brewer DN, Carr CM, Munson M. (2012). Regulation of exocytosis by the exocyst subunit Sec6 and the SM protein Sec1. Mol Biol Cell 23, 337-346.
26. Novick P, Botstein D. (1985). Phenotypic analysis of temperature-sensitive yeast actin mutants. Cell 40, 405-416.
27. Picco A, Irastorza-Azcarate I, Specht T, Boke D, Pazos I, Rivier-Cordey AS, Devos D P, Kaksonen M, Gallego O. (2017). The in vivo architecture of the exocyst provides structural basis of exocytosis. Cell 168, 400-412e418.
28. Pringle JR, Adams AE, Drubin DG, Haarer B.K. (1991). Immunofluorescence methods of yeast. Methods Enzymol 194, 565-602.
29. Ren Y, Yip CK, Tripathi A, Huie D, Jeffrey PD, Walz T, Hughson F.M. (2009). A structure-based mechanism of vesicle capture by the multisubunit tethering complex Dsl1. Cell 139, 1119-1129.
30. Roumanie O, Wu H, Molk JN, Rossi G, Bloom K, Brennwald P. (2005). Rho GTPase regulation of exocytosis in yeast is independent of GTP hydrolysis and polarization of the exocyst complex. J Cell Biol 170, 583-594.
31. Ruohola H, Ferro-Novick S (1987). Sec53, a protein required of an early step in secretory protein processing and transport in yeast, interacts with the cytoplasmic surface of the endoplasmic reticulum. Proc Natl Acad Sci USA 84, 8468-8472.
32. Shen D, Yuan H, Hutagalung A, Verma A, Kummel D, Wu X, Reinisch K, McNew JA, Novick P. (2013). The synaptobrevin homologue Snc2p recruits the exocyst to secretory vesicles by binding to Sec6p. J Cell Biol 202, 509-526.
33. Stimpson HE, Toret C P, Cheng AT, Pauly BS, Drubin D.G. (2009). Early-arriving Syp1p and Ede1p function in endocytic site placement and formation in budding yeast. Mol Biol Cell 20, 4640-4651.
34. TerBush DR, Maurice T, Roth D, Novick P. (1996). The Exocyst is a multiprotein complex required of exocytosis in Saccharomyces cerevisiae. EMBO J 15, 6483-6494.
35. TerBush DR, Novick P. (1995). Sec6, Sec8, and Sec15 are components of a multisubunit complex which localizes to small bud tips in Saccharomyces cerevisiae. J Cell Biol 130, 299-312.
36. Tripathi A, Ren Y, Jeffrey PD, Hughson F.M. (2009). Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nat Struct Mol Biol 16, 114-123.
37. Valdez-Taubas J, Pelham H.R. (2003). Slow diffusion of proteins in the yeast plasma membrane allows polarity to be maintained by endocytic cycling. Curr Biol 13, 1636-1640.
38. Vasan N, Hutagalung A, Novick P, Reinisch K.M. (2010). Structure of a C-terminal fragment of its Vps53 subunit suggests similarity of Golgi-associated retrograde protein (GARP) complex to a family of tethering complexes. Proc Natl Acad Sci USA 107, 14176-14181.
39. Walworth NC, Novick P.J. (1987). Purification and characterization of constitutive secretory vesicles from yeast. J Cell Biol 105, 163-174.
40. Wiederkehr A, De Craene JO, Ferro-Novick S, Novick P. (2004). Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p. J Cell Biol 167, 875-887.
41. Yu IM, Hughson F.M. (2010). Tethering factors as organizers of intracellular vesicular traffic. Annu Rev Cell Dev Biol 26, 137-156.
42. Yue P, Zhang Y, Mei K, Wang S, Lesigang J, Zhu Y, Dong G, Guo W. (2017). Sec3 promotes the initial binary t-SNARE complex assembly and membrane fusion. Nat Commun 8, 14236.
43. Zhang X, Bi E, Novick P, Du L, Kozminski KG, Lipschutz JH, Guo W. (2001). Cdc42 interacts with the exocyst and regulates polarized secretion. J Biol Chem 276, 46745-46750.
44. Zhang X, Orlando K, He B, Xi F, Zhang J, Zajac A, Guo W. (2008). Membrane association and functional regulation of Sec3 by phospholipids and Cdc42. J Cell Biol 180, 145-158.