Firefly Luciferase Mutant with Enhanced Activity and Thermostability

ACS Omega

Volumn 3, Issue 3, 2018, Pages 2628-2633

  Pozzo, Tania ^a   Akter, Farhima ^a   Nomura, Yoko ^b   Louie, Angelique Y ^a   Yokobayashi, Yohei ^b  

^a University of California Davis   (United States)

^b OKINAWA INSTITUTE OF SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 85043366374     PISSN: None     EISSN: 24701343     Source Type: Journal    
DOI: 10.1021/acsomega.7b02068     Document Type: Article

References (24)

1. Green, A. A.; McElroy, W. D. Crystalline firefly luciferase. Biochim. Biophys. Acta 1956, 20, 170-176.
2. Deluca, M.; McElroy, W. D. [1] Purification and properties of firefly luciferase. Methods Enzymol. 1978, 57, 3-15.
3. de Wet, J. R.; Wood, K. V.; Helinski, D. R.; DeLuca, M. Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 1985, 82, 7870-7873.
4. Conti, E.; Franks, N. P.; Brick, P. Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes. Structure 1996, 4, 287-298.
5. Kaskova, Z. M.; Tsarkova, A. S.; Yampolsky, I. V. 1001 lights: luciferins, luciferases, their mechanisms of action and applications in chemical analysis, biology and medicine. Chem. Soc. Rev. 2016, 45, 6048-6077.
6. Naylor, L. H. Reporter gene technology: the future looks bright. Biochem. Pharmacol. 1999, 58, 749-757.
7. Liu, A. M. F.; New, D. C.; Lo, R. K. H.; Wong, Y. H. Reporter gene assays. Methods Mol. Biol. 2009, 486, 109-123.
8. Niu, G.; Chen, X. Molecular imaging with activatable reporter systems. Theranostics 2012, 2, 413-423.
9. Baggett, B.; Roy, R.; Momen, S.; Morgan, S.; Tisi, L.; Morse, D.; Gillies, R. J. Thermostability of firefly luciferases affects efficiency of detection by in vivo bioluminescence. Mol. Imaging 2004, 3, 324-332.
10. Koksharov, M. I.; Ugarova, N. N. Approaches to engineer stability of beetle luciferases. Comput. Struct. Biotechnol. J. 2012, 2, No. e201204004.
11. Loening, A. M.; Walls, Z. F. Engineering Luciferases for Assays and Imaging. In Engineering in Translational Medicine; Cai, W., Eds., 1st ed.; Springer: London, 2014, pp 203-231.
12. Branchini, B. R.; Southworth, T. L.; Fontaine, D. M.; Kohrt, D.; Welcome, F. S.; Florentine, C. M.; Henricks, E. R.; DeBartolo, D. B.; Michelini, E.; Cevenini, L.; Roda, A.; Grossel, M. J. Red-emitting chimeric firefly luciferase for in vivo imaging in low ATP cellular environments. Anal. Biochem. 2017, 534, 36-39.
13. Reddy, G. R.; Thompson, W. C.; Miller, S. C. Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase. J. Am. Chem. Soc. 2010, 132, 13586-13587.
14. Evans, M. S.; Chaurette, J. P.; Adams, S. T., Jr.; Reddy, G. R.; Paley, M. A.; Aronin, N.; Prescher, J. A.; Miller, S. C. A synthetic luciferin improves bioluminescence imaging in live mice. Nat. Methods 2014, 11, 393-395.
15. Mofford, D. M.; Reddy, G. R.; Miller, S. C. Aminoluciferins extend firefly luciferase bioluminescence into the near-infrared and can be preferred substrates over D-luciferin. J. Am. Chem. Soc. 2014, 136, 13277-13282.
16. Wang, W.; Zhao, Q.; Luo, M.; Li, M.; Wang, D.; Wang, Y.; Liu, Q. Immobilization of Firefly Luciferase on PVA-co-PE Nanofibers Membrane as Biosensor for Bioluminescent Detection of ATP. ACS Appl. Mater. Interfaces 2015, 7, 20046-20052.
17. Alam, R.; Zylstra, J.; Fontaine, D. M.; Branchini, B. R.; Maye, M. M. Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins. Nanoscale 2013, 5, 5303-5306.
18. Alam, R.; Karam, L. M.; Doane, T. L.; Coopersmith, K.; Fontaine, D. M.; Branchini, B. R.; Maye, M. M. Probing Bioluminescence Resonance Energy Transfer in Quantum Rod-Luciferase Nanoconjugates. ACS Nano 2016, 10, 1969-1977.
19. Noda, K.; Matsuno, T.; Fujii, H.; Kogure, T.; Urata, M.; Asami, Y.; Kuroda, A. Single bacterial cell detection using a mutant luciferase. Biotechnol. Lett. 2008, 30, 1051-1054.
20. Fujii, H.; Noda, K.; Asami, Y.; Kuroda, A.; Sakata, M.; Tokida, A. Increase in bioluminescence intensity of firefly luciferase using genetic modification. Anal. Biochem. 2007, 366, 131-136.
21. Branchini, B. R.; Ablamsky, D. M.; Murtiashaw, M. H.; Uzasci, L.; Fraga, H.; Southworth, T. L. Thermostable red and green lightproducing firefly luciferase mutants for bioluminescent reporter applications. Anal. Biochem. 2007, 361, 253-262.
22. DeLuca, M.; McElroy, W. D. Kinetics of the firefly luciferase catalyzed reactions. Biochemistry 1974, 13, 921-925.
23. Franks, N. P.; Jenkins, A.; Conti, E.; Lieb, W. R.; Brick, P. Structural basis for the inhibition of firefly luciferase by a general anesthetic. Biophys. J. 1998, 75, 2205-2211.
24. Branchini, B. R.; Southworth, T. L.; Khattak, N. F.; Michelini, E.; Roda, A. Red-and green-emitting firefly luciferase mutants for bioluminescent reporter applications. Anal. Biochem. 2005, 345, 140-148.