Comparative analysis of EV isolation procedures for miRNAs detection in serum samples

Journal of Extracellular Vesicles

Volumn 5, Issue 1, 2016, Pages

  Andreu, Zoraida ^a   Rivas, Eva ^b   Sanguino Pascual, Aitana ^b   Lamana, Amalia ^b   Marazuela, Mónica ^b   González Alvaro, Isidoro ^b   Sánchez Madrid, Francisco ^b   de la Fuente, Hortensia ^b   Yáñez Mó, María ^a,c  

^a HOSPITAL UNIVERSITARIO SANTA CRISTINA   (Spain)

^b Instituto de Investigación Sanitaria del Hospital Universitario de La Princesa   (Spain)

^c CENTRO DE BIOLOGÍA MOLECULAR SEVERO OCHOA   (Spain)

Author keywords

biomarker; diagnosis; extracellular vesicles; microRNA; polyethylene glycol; serum

Indexed keywords

MACROGOL; MICRORNA; MICRORNA 126; MICRORNA 143; MICRORNA 16 5P; MICRORNA 19A; MICRORNA 21; MICRORNA 30C; NANOPARTICLE; UNCLASSIFIED DRUG;

ARTICLE; BLOOD SAMPLING; CELL ENCAPSULATION; CELL FRACTIONATION; CELL ISOLATION; COHORT ANALYSIS; COMPARATIVE STUDY; CONTROLLED STUDY; CYTOMETRY; EXOSOME; HUMAN; HUMAN CELL; LONGITUDINAL STUDY; POLYMERASE CHAIN REACTION; PRECIPITATION; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; RNA ANALYSIS; SOFTWARE; STORAGE; ULTRACENTRIFUGATION; WESTERN BLOTTING;

EID: 85042675886     PISSN: None     EISSN: 20013078     Source Type: Journal    
DOI: 10.3402/jev.v5.31655     Document Type: Article

References (27)

1. Negrini M, Nicoloso MS, Calin GA. MicroRNAs and cancer–new paradigms in molecular oncology. Curr Opin Cell Biol. 2009; 21: 470–9.
2. Reid G, Kirschner MB, van Zandwijk N. Circulating microRNAs: association with disease and potential use as biomarkers. Crit Rev Oncol Hematol. 2011; 80: 193–208.
3. Chen X, Ba Y, Ma L, Cai X, Yin Y, Wang K, etal. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Res. 2008; 18: 997–1006.
4. Bala S, Petrasek J, Mundkur S, Catalano D, Levin I, Ward J, etal. Circulating microRNAs in exosomes indicate hepatocyte injury and inflammation in alcoholic, drug-induced, and inflammatory liver diseases. Hepatology. 2012; 56: 1946–57.
5. Laterza OF, Lim L, Garrett-Engele PW, Vlasakova K, Muniappa N, Tanaka WK, etal. Plasma microRNAs as sensitive and specific biomarkers of tissue injury. Clin Chem. 2009; 55: 1977–83.
6. Scholer N, Langer C, Dohner H, Buske C, Kuchenbauer F. Serum microRNAs as a novel class of biomarkers: a comprehensive review of the literature. Exp Hematol. 2010; 38: 1126–30.
7. Vallelunga A, Ragusa M, Di Mauro S, Iannitti T, Pilleri M, Biundo R, etal. Identification of circulating microRNAs for the differential diagnosis of Parkinson's disease and multiple system atrophy. Front Cell Neurosci. 2014; 8: 156.
8. Pivarcsi A, Meisgen F, Xu N, Stahle M, Sonkoly E. Changes in the level of serum microRNAs in patients with psoriasis after antitumour necrosis factor-alpha therapy. Br J Dermatol. 2013; 169: 563–70.
9. Castro-Villegas C, Perez-Sanchez C, Escudero A, Filipescu I, Verdu M, Ruiz-Limon P, etal. Circulating miRNAs as potential biomarkers of therapy effectiveness in rheumatoid arthritis patients treated with anti-TNFalpha. Arthritis Res Ther. 2015; 17: 49.
10. Jansen F, Yang X, Proebsting S, Hoelscher M, Przybilla D, Baumann K, etal. MicroRNA expression in circulating microvesicles predicts cardiovascular events in patients with coronary artery disease. J Am Heart Assoc. 2014; 3: e001249.
11. Yanez-Mo M, Siljander PR, Andreu Z, Zavec AB, Borras FE, Buzas EI, etal. Biological properties of extracellular vesicles and their physiological functions. J Extracell Vesicles. 2015; 4: 27066.
12. Thery C, Amigorena S, Raposo G, Clayton A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol. 2006; Chapter 3: Unit 3 22.
13. Kordelas L, Rebmann V, Ludwig AK, Radtke S, Ruesing J, Doeppner TR, etal. MSC-derived exosomes: a novel tool to treat therapy-refractory graft-versus-host disease. Leukemia. 2014; 28: 970–3.
14. Yanez-Mo M, Alfranca A, Cabanas C, Marazuela M, Tejedor R, Ursa MA, etal. Regulation of endothelial cell motility by complexes of tetraspan molecules CD81/TAPA-1 and CD151/PETA-3 with alpha3 beta1 integrin localized at endothelial lateral junctions. J Cell Biol. 1998; 141: 791–804.
15. Gonzalez-Alvaro I, Ortiz AM, Alvaro-Gracia JM, Castaneda S, Diaz-Sanchez B, Carvajal I, etal. Interleukin 15 levels in serum may predict a severe disease course in patients with early arthritis. PLoS One. 2011; 6: e29492.
16. Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ. Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. J Biol Chem. 1998; 273: 20121–7.
17. Raposo G, Stoorvogel W. Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol. 2013; 200: 373–83.
18. Akbas F, Coskunpinar E, Aynaci E, Oltulu YM, Yildiz P. Analysis of serum micro-RNAs as potential biomarker in chronic obstructive pulmonary disease. Exp Lung Res. 2012; 38: 286–94.
19. Farina NH, Wood ME, Perrapato SD, Francklyn CS, Stein GS, Stein JL, etal. Standardizing analysis of circulating microRNA: clinical and biological relevance. J Cell Biochem. 2014; 115: 805–11.
20. Wang K, Zhang S, Weber J, Baxter D, Galas DJ. Export of microRNAs and microRNA-protective protein by mammalian cells. Nucleic Acids Res. 2010; 38: 7248–59.
21. Spinetti G, Fortunato O, Caporali A, Shantikumar S, Marchetti M, Meloni M, etal. MicroRNA-15a and microRNA-16 impair human circulating proangiogenic cell functions and are increased in the proangiogenic cells and serum of patients with critical limb ischemia. Circ Res. 2013; 112: 335–46.
22. Alegre E, Sanmamed MF, Rodriguez C, Carranza O, Martin-Algarra S, Gonzalez A. Study of circulating microRNA-125b levels in serum exosomes in advanced melanoma. Arch Pathol Lab Med. 2014; 138: 828–32.
23. Pfeffer SR, Grossmann KF, Cassidy PB, Yang CH, Fan M, Kopelovich L, etal. Detection of exosomal miRNAs in the plasma of melanoma patients. J Clin Med. 2015; 4: 2012–27.
24. Hulsmans M, Holvoet P. MicroRNA-containing microvesicles regulating inflammation in association with atherosclerotic disease. Cardiovasc Res. 2013; 100: 7–18.
25. Ramezani A, Devaney JM, Cohen S, Wing MR, Scott R, Knoblach S, etal. Circulating and urinary microRNA profile in focal segmental glomerulosclerosis: a pilot study. Eur J Clin Invest. 2015; 45: 394–404.
26. Li Y, Zhang L, Liu F, Xiang G, Jiang D, Pu X. Identification of endogenous controls for analyzing serum exosomal miRNA in patients with hepatitis B or hepatocellular carcinoma. Dis Markers. 2015; 2015: 893594.
27. Ge Q, Zhou Y, Lu J, Bai Y, Xie X, Lu Z. miRNA in plasma exosome is stable under different storage conditions. Molecules. 2014; 19: 1568–75.