Comparison of two hyoscyamine 6β-hydroxylases in engineering scopolamine biosynthesis in root cultures of Scopolia lurida

Biochemical and Biophysical Research Communications

Volumn 497, Issue 1, 2018, Pages 25-31

  Lan, Xiaozhong ^a,b,c   Zeng, Junlan ^b   Liu, Ke ^b   Zhang, Fangyuan ^b   Bai, Ge ^d   Chen, Min ^b   Liao, Zhihua ^b,c   Huang, Luqi ^a  

^a INSTITUTE OF CHINESE MATERIA MEDICA   (China)

^b SOUTHWEST UNIVERSITY   (China)

^c Research Institute of Plateau Ecology Tibet Agriculture and Animal Husbandry College   (China)

^d Tobacco Breeding and Biotechnology Research Center   (China)

Author keywords

Biosynthesis; H6H; Hyoscyamus niger; Metabolic engineering; Scopolia lurida; Tropane alkaloids

Indexed keywords

ALKALOID DERIVATIVE; ANISODAMINE; HYOSCYAMINE; HYOSCYAMINE 6BETA HYDROXYLASE; OXYGENASE; PLANT EXTRACT; PLANT PROTEIN; SCOPOLAMINE; SCOPOLIA LURIDA EXTRACT; TROPANE ALKALOID; UNCLASSIFIED DRUG; HYOSCYAMINE (6S)-DIOXYGENASE; MIXED FUNCTION OXIDASE; RECOMBINANT PROTEIN; SCOPOLAMINE BROMIDE;

ARTICLE; BIOENGINEERING; BIOSYNTHESIS; CHEMICAL COMPOSITION; COMPARATIVE STUDY; CONTROLLED STUDY; DRUG SYNTHESIS; FEASIBILITY STUDY; GENE OVEREXPRESSION; GENETIC TRANSCRIPTION; HAIRY ROOT CULTURE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYOSCYAMINE 6BETA HYDROXYLASE GENE; HYOSCYAMUS; MOLECULAR CLONING; NONHUMAN; PLANT GENE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SCOPOLIA; SCOPOLIA LURIDA; ENZYME ACTIVATION; ENZYME STABILITY; EVALUATION STUDY; GENETIC ENHANCEMENT; GENETICS; ISOLATION AND PURIFICATION; METABOLIC ENGINEERING; METABOLISM; PHYSIOLOGY; PLANT ROOT; PROCEDURES; TRANSGENIC PLANT;

ENZYME ACTIVATION; ENZYME STABILITY; GENETIC ENHANCEMENT; METABOLIC ENGINEERING; MIXED FUNCTION OXYGENASES; PLANT ROOTS; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; SCOPOLAMINE HYDROBROMIDE; SCOPOLIA;

EID: 85041751359     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2018.01.173     Document Type: Article

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