Impact of Inverted Terminal Repeat Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System

Human Gene Therapy Methods

Volumn 28, Issue 5, 2017, Pages 277-289

  Savy, Adrien ^a,b   Dickx, Yohann ^a   Nauwynck, Lucile ^a   Bonnin, Delphine ^a   Merten, Otto Wilhelm ^a   Galibert, Lionel ^a,c  

^a GÉNÉTHON   (France)

^b UNIVERSITÉ D EVRY VAL D ESSONNE   (France)

^c PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United Kingdom)

Author keywords

baculovirus; ITR; quality; rAAV

Indexed keywords

CIS ACTING ELEMENT; DNA POLYMERASE; NUCLEOTIDE; RECOMBINANT DNA; RESTRICTION ENDONUCLEASE; VIRUS DNA;

ADENO ASSOCIATED VIRUS 8; AMPLICON; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CONTROLLED STUDY; DNA FLANKING REGION; DNA SEQUENCE; GENE REPLICATION; GENETIC TRANSFECTION; INVERTED TERMINAL REPEAT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RECOMBINANT GENE; SEROTYPE; SF9 CELL LINE; SHUTTLE VECTOR; TRANSGENE; VIRUS CAPSID; VIRUS GENOME; VIRUS RECOMBINANT; VIRUS TITRATION; ANIMAL; BACULOVIRIDAE; DEPENDOPARVOVIRUS; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; LONG TERMINAL REPEAT; METABOLISM; REAL TIME POLYMERASE CHAIN REACTION; SPODOPTERA; ULTRACENTRIFUGATION; WESTERN BLOTTING;

ANIMALS; BACULOVIRIDAE; BLOTTING, WESTERN; DEPENDOVIRUS; DNA, VIRAL; GENETIC VECTORS; REAL-TIME POLYMERASE CHAIN REACTION; SF9 CELLS; SPODOPTERA; TERMINAL REPEAT SEQUENCES; ULTRACENTRIFUGATION;

EID: 85032866952     PISSN: 19466536     EISSN: 19466544     Source Type: Journal    
DOI: 10.1089/hgtb.2016.133     Document Type: Article

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