Decreased intra-lymphocyte cytokines measurement in septic shock patients: A proof of concept study in whole blood

Cytokine

Volumn 104, Issue , 2018, Pages 78-84

  Letessier, Wilfried ^a   Demaret, Julie ^b,c   Gossez, Morgane ^b,c   Allam, Camille ^b,c   Venet, Fabienne ^b,c   Rimmelé, Thomas ^b,c   Monneret, Guillaume ^b,c  

^a Hôpital Inter Armées du Val de Grâce   (France)

^b EDOUARD HERRIOT HOSPITAL   (France)

^c University of Lyon EA 7426 Pathophysiology of Injury Induced Immunosuppression PI3   (France)

Author keywords

Flow cytometry; Intracellular cytokine; Sepsis; Septic shock; T lymphocyte

Indexed keywords

CD4 ANTIGEN; CD8 ANTIGEN; GAMMA INTERFERON; INTERLEUKIN 2; TUMOR NECROSIS FACTOR; CYTOKINE;

AGED; ARTICLE; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CELL LEVEL; CHEMICAL LABELING; CLINICAL ARTICLE; CONTROLLED STUDY; CYTOKINE PRODUCTION; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LYMPHOCYTOPENIA; MALE; PRIORITY JOURNAL; PROTEIN BLOOD LEVEL; SEPTIC SHOCK; T LYMPHOCYTE ACTIVATION; BLOOD; METABOLISM; MIDDLE AGED; VERY ELDERLY;

AGED; AGED, 80 AND OVER; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CYTOKINES; FEMALE; HUMANS; MALE; MIDDLE AGED; SHOCK, SEPTIC;

EID: 85030249416     PISSN: 10434666     EISSN: 10960023     Source Type: Journal    
DOI: 10.1016/j.cyto.2017.09.029     Document Type: Article

References (15)

1. Notarangelo, L.D., Fischer, A., Geha, R.S., Casanova, J.L., Chapel, H., Conley, M.E., Cunningham-Rundles, C., Etzioni, A., Hammartrom, L., Nonoyama, S., et al. Primary immunodeficiencies: 2009 update. J. Allergy Clin. Immunol. 124:6 (2009), 1161–1178.
2. Notarangelo, L.D., Functional T cell immunodeficiencies (with T cells present). Annu. Rev. Immunol. 31 (2013), 195–225.
3. Butterfield, L.H., Palucka, A.K., Britten, C.M., Dhodapkar, M.V., Hakansson, L., Janetzki, S., Kawakami, Y., Kleen, T.O., Lee, P.P., Maccalli, C., et al. Recommendations from the iSBTc-SITC/FDA/NCI Workshop on Immunotherapy Biomarkers. Clin Cancer Res 17:10 (2011), 3064–3076.
4. Freer, G., Rindi, L., Intracellular cytokine detection by fluorescence-activated flow cytometry: basic principles and recent advances. Methods 61:1 (2013), 30–38.
5. Finco, D., Grimaldi, C., Fort, M., Walker, M., Kiessling, A., Wolf, B., Salcedo, T., Faggioni, R., Schneider, A., Ibraghimov, A., et al. Cytokine release assays: current practices and future directions. Cytokine 66:2 (2014), 143–155.
6. John, J., Pandha, H., Dalgleish, A.G., Detection of T cell cytokine production as a tool for monitoring immunotherapy. Dis. Mark. 16:1–2 (2000), 71–75.
7. Monneret, G., Demaret, J., Gossez, M., Reverdiau, E., Malergue, F., Rimmele, T., Venet, F., Novel approach in monocyte intracellular TNF measurement: application to sepsis-induced immune alterations. Shock 47:3 (2016), 318–322.
8. Hotchkiss, R.S., Monneret, G., Payen, D., Sepsis-induced immunosuppression: from cellular dysfunctions to immunotherapy. Nat. Rev. Immunol. 13:12 (2013), 862–874.
9. Singer, M., Deutschman, C.S., Seymour, C.W., Shankar-Hari, M., Annane, D., Bauer, M., Bellomo, R., Bernard, G.R., Chiche, J.D., Coopersmith, C.M., et al. The third international consensus definitions for sepsis and septic shock (Sepsis-3). JAMA 315:8 (2016), 801–810.
10. Freer, G., Intracellular staining and detection of cytokines by fluorescence-activated flow cytometry. Methods Mol. Biol. 1172 (2014), 221–234.
11. Pala, P., Hussell, T., Openshaw, P.J., Flow cytometric measurement of intracellular cytokines. J. Immunol. Methods 243:1–2 (2000), 107–124.
12. Monneret, G., Venet, F., Pachot, A., Lepape, A., Monitoring immune dysfunctions in the septic patient: a new skin for the old ceremony. Mol. Med. 14:1–2 (2008), 64–78.
13. Wherry, E.J., T cell exhaustion. Nat. Immunol. 12:6 (2011), 492–499.
14. Venet, F., Foray, A.P., Villars-Mechin, A., Malcus, C., Poitevin-Later, F., Lepape, A., Monneret, G., IL-7 restores lymphocyte functions in septic patients. J. Immunol. 189:10 (2012), 5073–5081.
15. Snyder, L.D., Chan, C., Kwon, D., Yi, J.S., Martissa, J.A., Copeland, C.A., Osborne, R.J., Sparks, S.D., Palmer, S.M., Weinhold, K.J., Polyfunctional T-cell signatures to predict protection from cytomegalovirus after lung transplantation. Am. J. Respir. Crit. Care Med. 193:1 (2016), 78–85.