Expansion mini-microscopy: An enabling alternative in point-of-care diagnostics

Current Opinion in Biomedical Engineering

Volumn 1, Issue , 2017, Pages 45-53

  Zhang, Yu Shrike ^a,b,c   Trujillo de Santiago, Grissel ^a,b,d,e   Alvarez, Mario Moisés ^a,b,d,e   Schiff, Steven J ^f,g,h   Boyden, Edward S ^e,i,j   Khademhosseini, Ali ^a,b,k,l  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

^b HARVARD MIT DIVISION OF HEALTH SCIENCES AND TECHNOLOGY   (United States)

^c HARVARD UNIVERSITY   (United States)

^d SCHOOL OF ENGINEERING AND SCIENCES   (Mexico)

^e Massachusetts Institute of Technology   (United States)

^f The Pennsylvania State University   (United States)

^g PENN STATE COLLEGE OF MEDICINE   (United States)

^h PENNSYLVANIA STATE UNIVERSITY   (United States)

ⁱ MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^j MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^k Konkuk University   (South Korea)

^l KING ABDULAZIZ UNIVERSITY   (Saudi Arabia)

more..

Author keywords

Expansion microscopy; Low cost; Miniature imager; Point of care; Portable

Indexed keywords

COST CONTROL; DIAGNOSTIC IMAGING; EQUIPMENT DESIGN; EXPANSION MICROSCOPY; EXPANSION MINI MICROSCOPY; HEALTH CARE PLANNING; HUMAN; IMAGE ENHANCEMENT; IMAGE QUALITY; LABORATORY TECHNIQUE; MICROSCOPY; NONHUMAN; POINT OF CARE TESTING; PRODUCT DEVELOPMENT; REVIEW; TECHNOLOGY;

EID: 85023165272     PISSN: None     EISSN: 24684511     Source Type: Journal    
DOI: 10.1016/j.cobme.2017.03.001     Document Type: Review

References (47)

1. Gubala, V., Harris, L.F., Ricco, A.J., Tan, M.X., Williams, D.E., Point of care diagnostics: status and future. Anal. Chem. 84 (2011), 487–515.
2. Pai, N.P., Vadnais, C., Denkinger, C., Engel, N., Pai, M., Point-of-care testing for infectious diseases: diversity, complexity, and barriers in low-and middle-income countries. Plos Med., 9, 2012, e1001306.
3. Lee, W.G., Kim, Y.-G., Chung, B.G., Demirci, U., Khademhosseini, A., Nano/Microfluidics for diagnosis of infectious diseases in developing countries. Adv. Drug Deliv. Rev. 62 (2010), 449–457.
4. Ozcan, A., Demirci, U., Ultra wide-field lens-free monitoring of cells on-chip. Lab. Chip 8 (2008), 98–106.
5. Greenbaum, A., Zhang, Y., Feizi, A., Chung, P.-L., Luo, W., Kandukuri, S.R., Ozcan, A., Wide-field computational imaging of pathology slides using lens-free on-chip microscopy. Sci. Transl. Med., 6, 2014 267ra175-267ra175.
6. Coskun, A.F., Sencan, I., Su, T.-W., Ozcan, A., Wide-field lensless fluorescent microscopy using a tapered fiber-optic faceplate on a chip. Analyst 136 (2011), 3512–3518.
7. Cybulski, J.S., Clements, J., Prakash, M., Foldscope: origami-based paper microscope. Plos One, 2014(9), 2014, e98781.
8. Ghosh, K.K., Burns, L.D., Cocker, E.D., Nimmerjahn, A., Ziv, Y., El Gamal, A., Schnitzer, M.J., Miniaturized integration of a fluorescence microscope. Nat. Methods 8 (2011), 871–878.
9. Mudanyali, O., Tseng, D., Oh, C., Isikman, S.O., Sencan, I., Bishara, W., Oztoprak, C., Seo, S., Khademhosseini, B., Ozcan, A., Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications. Lab. Chip 10 (2010), 1417–1428.
10. Kim, S.B., Bae, H., Koo, K.-I., Dokmeci, M.R., Ozcan, A., Khademhosseini, A., Lens-free imaging for biological applications. J. Lab. Autom. 17 (2012), 43–49.
11. Chen, F., Tillberg, P.W., Boyden, E.S., Expansion microscopy. Science, 347, 2015, 1260088 This paper denotes the invention of the groundbreaking technology of expansion microscopy, where the authors showed nanoscale imaging using conventional diffraction-limited optical microscopy through physical expansion of biological specimens.
12. Chen, F., Wassie, A.T., Cote, A.J., Sinha, A., Alon, S., Asano, S., Daugharthy, E.R., Chang, J.-B., Marblestone, A., Church, G.M., Nanoscale imaging of RNA with expansion microscopy. Nat. Methods 13 (2016), 679–684 The authors extend the application of the original expansion microscopy to high-resolution imaging of nucleic acids.
13. Tillberg, P.W., Chen, F., Piatkevich, K.D., Zhao, Y., Yu, C.-C.J., English, B.P., Gao, L., Martorell, A., Suk, H.-J., Yoshida, F., Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies. Nat. Biotechnol. 34 (2016), 987–992 The authors extend the application of the original expansion microscopy to high-resolution imaging of proteins.
14. Zhang, Y.S., Chang, J.-B., Alvarez, M.M., Trujillo-de Santiago, G., Aleman, J., Batzaya, B., Krishnadoss, V., Ramanujam, A.A., Kazemzadeh-Narbat, M., Chen, F., Hybrid microscopy: enabling inexpensive high-performance imaging through combined physical and optical magnifications. Sci. Rep., 6, 2016, 22691 This paper provides the first example of the combination of expansion microscopy with mini-microscopy to image biological samples at high resolution with low cost.
15. Germroth, P.G., Gourdie, R.G., Thompson, R.P., Confocal microscopy of thick sections from acrylamide gel embedded embryos. Microsc. Res. Tech. 30 (1995), 513–520.
16. Rust, M.J., Bates, M., Zhuang, X., Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat. Methods 3 (2006), 793–796.
17. Hein, B., Willig, K.I., Hell, S.W., Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell. Proc. Natl. Acad. Sci. 105 (2008), 14271–14276.
18. Bucur, O., Zhao, Y., Boyden, E., Beck, A.H., Physical expansion of tissue microarrays for high-resolution imaging of normal and cancer samples with conventional microscopy. Cancer Res., 76, 2016 4229–4229.
19. Crittenden, J.R., Tillberg, P.W., Riad, M.H., Shima, Y., Gerfen, C.R., Curry, J., Housman, D.E., Nelson, S.B., Boyden, E.S., Graybiel, A.M., Striosome–dendron bouquets highlight a unique striatonigral circuit targeting dopamine-containing neurons. Proc. Natl. Acad. Sci. 113 (2016), 11318–11323.
20. Ku, T., Swaney, J., Park, J.-Y., Albanese, A., Murray, E., Cho, J.H., Park, Y.-G., Mangena, V., Chen, J., Chung, K., Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues. Nat. Biotechnol. 34 (2016), 973–981 This paper reports a variation of expansion microscopy based on a similar physical expansion principle.
21. Chozinski, T.J., Halpern, A.R., Okawa, H., Kim, H.-J., Tremel, G.J., Wong, R.O., Vaughan, J.C., Expansion microscopy with conventional antibodies and fluorescent proteins. Nat. Methods 13 (2016), 485–488 This paper has generalized the use of conventional antibodies and fluorescence proteins in expansion microscopy.
22. Lubeck, E., Cai, L., Single-cell systems biology by super-resolution imaging and combinatorial labeling. Nat. Methods 9 (2012), 743–748.
23. Lubeck, E., Coskun, A.F., Zhiyentayev, T., Ahmad, M., Cai, L., Single-cell in situ RNA profiling by sequential hybridization. Nat. Methods 11 (2014), 360–361.
24. Chen, K.H., Boettiger, A.N., Moffitt, J.R., Wang, S., Zhuang, X., Spatially resolved, highly multiplexed RNA profiling in single cells. Science, 348, 2015 aaa6090.
25. Moffitt, J.R., Hao, J., Wang, G., Chen, K.H., Babcock, H.P., Zhuang, X., High-throughput single-cell gene-expression profiling with multiplexed error-robust fluorescence in situ hybridization. Proc. Natl. Acad. Sci. 113 (2016), 11046–11051.
26. Shah, S., Lubeck, E., Zhou, W., Cai, L., In situ transcription profiling of single cells reveals spatial organization of cells in the mouse hippocampus. Neuron 92 (2016), 342–357.
27. Tsanov, N., Samacoits, A., Chouaib, R., Traboulsi, A.-M., Gostan, T., Weber, C., Zimmer, C., Zibara, K., Walter, T., Peter, M., smiFISH and FISH-quant–a flexible single RNA detection approach with super-resolution capability. Nucleic Acids Res., 2016 gkw784.
28. Zhu, H., Yaglidere, O., Su, T., Tseng, D., Ozcan, A., Cost-effective and compact wide-field fluorescent imaging on a cell-phone. Lab. Chip 11 (2011), 315–322.
29. Schaefer, S., Boehm, S., Chau, K., Automated, portable, low-cost bright-field and fluorescence microscope with autofocus and autoscanning capabilities. Appl. Opt. 51 (2012), 2581–2588.
30. Kim, S., Koo, K., Bae, H., Dokmeci, M., Hamilton, G., Bahinski, A., Kim, S., Ingber, D., Khademhosseini, A., A mini-microscope for in situ monitoring of cells. Lab. Chip 12 (2012), 3976–3982.
31. Zhang, Y., Ribas, J., Nadhman, A., Aleman, J., Selimović, Š., Lesher-Perez, S., Wang, T., Manoharan, V., Shin, S., Damilano, A., et al. A cost-effective fluorescence mini-microscope for biomedical applications. Lab. Chip 15 (2015), 3661–3669 Using the webcam-based mini-microscope, the authors describe portable imaging of biological samples at low cost.
32. Dong, S., Guo, K., Nanda, P., Shiradkar, R., Zheng, G., FPscope: a field-portable high-resolution microscope using a cellphone lens. Biomed. Opt. Express 5 (2014), 3305–33010.
33. Greenbaum, A., Sikora, U., Ozcan, A., Field-portable wide-field microscopy of dense samples using multi-height pixel super-resolution based lensfree imaging. Lab. Chip 12 (2012), 1242–1245.
34. Greenbaum, A., Akbari, N., Feizi, A., Luo, W., Ozcan, A., Field-portable pixel super-resolution colour microscope. Plos One, 8, 2013, e76475.
35. McLeod, E., Dincer, T., Veli, M., Ertas, Y., Nguyen, C., Luo, W., Greenbaum, A., Feizi, A., Ozcan, A., High-throughput and label-free single nanoparticle sizing based on time-resolved on-chip microscopy. ACS Nano 9 (2015), 3265–3273.
36. Wei, Q., Luo, W., Chiang, S., Kappel, T., Mejia, C., Tseng, D., Chan, R.Y.L., Yan, E., Qi, H., Shabbir, F., Imaging and sizing of single DNA molecules on a mobile phone. ACS Nano 8 (2014), 12725–12733.
37. Kühnemund, M., Wei, Q., Darai, E., Wang, Y., Hernández-Neuta, I., Yang, Z., Tseng, D., Ahlford, A., Mathot, L., Sjöblom, T., Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy. Nat. Commun., 8, 2017, 13913.
38. Miller, A., Davis, G., Oden, Z., Razavi, M., Fateh, A., Ghazanfari, M., Abdolrahimi, F., Poorazar, S., Sakhaie, F., Olsen, R., et al. Portable, battery-operated, low-cost, bright field and fluorescence microscope. Plos One, 5, 2010, e11890.
39. Bishara, W., Sikora, U., Mudanyali, O., Su, T., Yaglidere, O., Luckhart, S., Ozcan, A., Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array. Lab. Chip 11 (2011), 1276–1279.
40. Isikman, S., Bishara, W., Sikora, U., Yaglidere, O., Yeah, J., Ozcan, A., Field-portable lensfree tomographic microscope. Lab. Chip 11 (2011), 2222–2230.
41. Im, H., Castro, C., Shao, H., Liong, M., Song, J., Pathania, D., Fexon, L., Min, C., Avila-Wallace, M., Zurkiya, O., et al. Digital diffraction analysis enables low-cost molecular diagnostics on a smartphone. Proc. Natl. Acad. Sci. 112 (2015), 5613–5618.
42. Hutchison, J., Erikson, R., Sheen, A., Ozanich, R., Kelly, R., Reagent-free and portable detection of Bacillus anthracis spores using a microfluidic incubator and smartphone microscope. Analyst 140 (2015), 6269–6276.
43. Huang, H., Wei, K., Zhao, Y., Variable focus smartphone based microscope using an elastomer liquid lens. 2016 IEEE 29th International conference on micro electro mechanical systems (MEMS), 2016, IEEE.
44. Zhang, Y., Busignani, F., Ribas, J., Aleman, J., Rodrigues, T., Shaegh, S., Massa, S., Rossi, C., Taurino, I., Shin, S., et al. Google glass-directed monitoring and control of microfluidic biosensors and actuators. Sci. Rep., 6, 2016, 22237.
45. Bochyńska, A., Hannink, G., Grijpma, D., Buma, P., Tissue adhesives for meniscus tear repair: an overview of current advances and prospects for future clinical solutions. J. Mater. Sci. Mater. Med. 27 (2016), 1–18.
46. Pirnstill, C.W., Coté, G.L., Malaria diagnosis using a mobile phone polarized microscope. Sci. Rep., 5, 2015, 13368.
47. Cohen, Y., Ramon, O., Kopelman, I.J., Mizrahi, S., Characterization of inhomogeneous polyacrylamide hydrogels. J. Polym. Sci. Part B Polym. Phys. 30 (1992), 1055–1067.