Diagnosis of schistosomiasis: Antibody detection, with notes on parasitological and antigen detection methods

Parasitology

Volumn 117, Issue SUPPL., 1998, Pages

  Hamilton, J V ^a   Klinkert, M ^b   Doenhoff, M J ^a  

^a BANGOR UNIVERSITY   (United Kingdom)

^b CNR   (Italy)

Author keywords

Diagnosis; Immunodiagnosis; KLH; S. haematobium; S. japonicum; S. mansoni; Schistosomiasis; Serology

Indexed keywords

CROSS REACTING ANTIGEN; KEYHOLE LIMPET HEMOCYANIN; PARASITE ANTIBODY; PARASITE ANTIGEN;

ANTIBODY DETECTION; ANTIGEN DETECTION; CERCARIA; COST EFFECTIVENESS ANALYSIS; EGG; HUMAN; NONHUMAN; PARASITOLOGY; PRIORITY JOURNAL; PUBLIC HEALTH; RECOMBINANT DNA TECHNOLOGY; REVIEW; SCHISTOSOMIASIS; SERODIAGNOSIS; WORM;

ANIMALS; ANTIBODIES, HELMINTH; ANTIGENS, HELMINTH; HUMANS; IMMUNOASSAY; MICE; RATS; RECOMBINANT PROTEINS; SCHISTOSOMA; SCHISTOSOMIASIS;

SCHISTOSOMA; SCHISTOSOMIASIS MANSONI; TREMATODA;

EID: 85023006099     PISSN: 00311820     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (180)

1. AGNEW, A., FULFORD, A. J. C., DE JONGE, N., KRIJGER, F. W., RODRIQUEZCHACON, M., GUTSMANN, V. & DEELDER, A. M. (1995). The relationship between worm burden and levels of a circulating antigen (CAA) of 5 species of Schistosoma in mice. Parasitology 111, 67-76.
2. AGNEW, A. M., MURARE, H. M., SANDOVAL, S. N., DE JONGE, N., KRIJGER, F. W., DEELDER, A. M. & DOENHOFF, M. J. (1992). The susceptibility of adult schistosomes to immune attrition. Memorias do Instituto Oswaldo Cruz 87, 87-93.
3. ALVES-BRITO, C. F., SIMPSON, A. J. G., BAHIA-OLIVEIRA, L. M. G., RABELLO, A. L. T., ROCHA, R. S., LAMBERTUCCI, J. R., CAZINELLI, G., KATZ, N. & CORREA-OLIVEIRA, R. (1992). Analysis of anti-keyhole limpet haemocyanin antibody in Brazilians supports its use for the diagnosis of acute schistosomiasis mansoni. Transactions of the Royal Society of Tropical Medicine and Hygiene 86, 53-56.
4. AMBROISE-THOMAS, P. & ANDREWS, P. (1976). Development of fluorescent antibodies directed against larval stages, eggs, and adults of Schistosoma mansoni in mice harbouring unisexual or bisexual infections. Tropenmedizin und Parasitologie 27, 483-488.
5. AURIAULT, C., GRAS-MASSE, H., PIERCE, R. J., BUTTERWORTH, A. E., WOLOWCZUK, I., CAPRON, M., OUMA, J. H., BALLOUL, J. M., KHALIFE, J., NEYRINCK, J. L., TARTAR, A., KOECH, D. & CAPRON, A. (1990). Antibody response of Schistosoma mansoni-infected human subjects to the recombinant P28 glutathione S-transferasc and to synthetic peptides. Journal of Clinical Microbiology 28, 1918-1924.
6. BARRETO, M. L., FRANCA SILVA, J. T., MOTT, K. E. & LEHMAN, J. S. (1978). Stability of faecal egg excretion in Schistosoma mansoni infection. Transactions of the Royal Society of Tropical Medicine and Hygiene 72, 181-187.
7. BARRETO, M. L., SMITH, D. H. & SLEIGH, A. C. (1990). Implications of faecal egg count variation when using the Kato-Katz method to assess Schistosoma mansoni infections. Transactions of the Royal Society of Tropical Medicine and Hygiene 84, 554-555.
8. BELL, D. R. (1963). A new method for counting Schistosoma mansoni eggs in faeces. Bulletin of the World Health Organisation 29, 525-530.
9. BERGGREN, W. L. & WELLER, T. H. (1967). Immunoelectrophoretic demonstration of specific circulating antigen in animals infected with Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 16, 606-612.
10. BERGQUIST, N. R. (1992). Immunodiagnostic Approaches in Schistosomiasis. Chichester, John Wiley & Sons.
11. BERGWERFF, A. A., VAN DAM, G. J., ROTMANS, J. P., DEELDER, A. M., KAMERLING, J. P. & VLIEGENTHART, J. F. G. (1994). The immunologically reactive part of immunopurified circulating anodic antigen from Schistosoma mansoni is a threonine-linked polysaccharide consisting of → 6)-(beta-D-GlcpA-(1 → 3))-beta-D-GalpNAc-(1 → repeating units. Journal of Biological Chemistry 269, 31510-31517.
12. BOCTOR, F. N., STEK, M. J., PETER, J. B. & KAMAL, R. (1987). Simplification and standardisation of dot-ELISA for human schistosomiasis mansoni. Journal of Parasitology 73, 589-392.
13. BOGERS, J. J. P. M., NIBBELEING, H. A. M., VAN MARCK, E. A. E. & DEELDER, A. M. (1995). Immunoelectron microscopic localization of a circulating antigen in the excretory system of Schistosoma mansoni - ultra-structural-localization studies of the excretory system of Schistosoma mansoni. Parasitology Research 81, 375-381.
14. BRAUN-MUNZINGER, R. A. (1986). Quantitative egg counts in schistosomiasis surveys. Parasitology Today 2, 82-83.
15. CARLIER, Y., BOUT, D., BINA, J. C., CAMUS, D., FIGUEIREDO, J. F. M. & CAPRON, A. (1975). Immunological studies in human schistosomiasis. I. Parasitic antigen in urine. American Journal of Tropical Medicine and Hygiene 24, 949-954.
16. CESARI, I. M., FERRER, A., KOMBILA, M., PICHARD, E., DECAM, C., LI-SHIU, Q., BOUT, D. & RICHARD-LENOBLE, D. (1998). Specificity of the solid phase alkaline phosphatase immunocapture assay for the diagnosis of human Schistosoma mansoni infection. Transactions of the Royal Society of Tropical Medicine and Hygiene 92, 38-39.
17. CHAPPELL, C. L., DRESDEN, M. H., GRYSEELS, B. & DEEEDER, A. M. (1990). Antibody response to Schistosoma mansoni adult worm cysteine proteinases in infected individuals. American Journal of Tropical Medicine and Hygiene 42, 335-341.
18. CHEEVER, A. W. (1968). A quantitative post-mortem study of schistosomiasis mansoni in man. American Journal of Tropical Medicine and Hygiene 17, 38-64.
19. CHI, F. & CARTER, C. E. (1990). Detection of a circulating antigen in human schistosomiasis japonica using a monoclonal antibody. American Journal of Tropical Medicine and Hygiene 42, 347-351.
20. DAVERN, K. M., TIU, W. U., SAMARAS, N., GEARING, D. P., HALL, B. E., GARCIA, E. G. & MITCHELL, G. F. (1990). Schistosoma japonicum - monoclonal antibodies to the MR 26,000 schistosome glutathione S-transferase (Sj26) in an assay for circulating antigen in infected individuals. Experimental Parasitology 70, 293-304.
21. DAY, J. H., GRANT, A. H., DOHERTY, J. F., CHIODINI, P. L. & WRIGHT, S. G. (1996). Schistosomiasis in travellers returning from sub-Saharan Africa. British Medical Journal 313, 268-269.
22. DAZO, B. C. & BILES, J. E. (1974). Two new field techniques for detection and counting of Schistosoma haematobium eggs in urine samples, with an evaluation of both methods. Bulletin of the World Health Organisation 51, 399-408.
23. DE CLERCQ, D., SACKO, M., VERCRUYSSE, J., DIARRA, A., LANDOURE, A., VAN DEN BUSSCHE, V., GRYSEELS, B. & DEELDER, A. (1995). Comparison of the circulating anodic antigen detection assay and urine filtration to diagnose Schistosoma haematobium infections in Mali. Transactions of the Royal Society for Tropical Medicine and Hygiene 89, 395-397.
24. DE CLERQ, D., SACKO, M., VERCRUYSSE, J., VAN DEN BUSSCHE, V., LANDOURE, A., DIARRA, A., GRYSEELS, B. & DEELDER, A. (1997). Assessment of cure by detection of circulating antigens in serum and urine, following schistosomiasis mass treatment in two villages of the office du Niger, Mali. Acta Tropica 68, 339-346.
25. DEELDER, A. M., TIE JONGE, N., BOERMAN, O. C., FILLIE, Y. E., HILBERATH, J., ROTMANS, J. P., GERRITSE, M. J. & SCHUT, D. W. O. (1989a). Sensitive determination of circulating anodic antigen in Schistosoma mansoni infected individuals by an enzyme-linked immunosorbent assay using monoclonal antibodies. American Journal of Tropical Medicine and Hygiene 40, 268-272.
26. DEELDER, A. M., DE JONGE, N., FILLIE, Y. E., KORNELIS, D., HLAHA, D., QIAN, Z. L., DECALUWE, P. & POLDERMAN, A. M. (1989b). Quantitative determination of circulating antigens in human schistosomiasis mansoni using an indirect haemagglutination assay. American Journal of Tropical Medicine and Hygiene 40, 50-54.
27. DEELDER, A. M., KLAPPE, H. T. M., VAN DEN AARDWEG, G. J. M. J. & VAN MEERBEKE, E. H. E. M. (1976). Schistosoma mansoni: demonstration of two circulating antigens in hamsters. Experimental Parasitology 40, 189-197.
28. DEELDER, A. M. & KORNELIS, D. (1980). A comparison of the IFA and the ELISA for the demonstration of an antibody against schistosome gut-associated polysaccaride antigens in schistosomiasis. Zietschrift für Parasitenkunde 64, 65-75.
29. DEELDER, A. M. & KORNELIS, D. (1981). Immunodiagnosis of recently acquired Schistosoma mansoni infection. A comparison of various immunological techniques. Tropical and Geographical Medicine 33, 36-11.
30. DEELDER, A. M., KORNELIS, D., VAN MARCK, E. A. E., EVELEIGH, P. C. & VAN EGMOND, J. G. (1980). Schistosoma mansoni: characterization of two circulating polysaccharide antigens and the immunological response to these antigens in mouse, hamster and human infections. Experimental Parasitology 50, 16-32.
31. DEELDER, A. M., QIAN, Z. L., KREMSNER, P. G., ACOSTA, L., RABELLO, A. L. T., ENYONG, P., SIMARRO, P. P., VAN ETTEN, E. C. M., KRIJGER, F. W., ROTMANS, J. P., FILLIE, Y. E., DE JONGE, N., AGNEW, A. M. & VAN LIESHOUT, L. (1994). Quantitative diagnosis of Schistosoma infections by measurement of circulating antigens in serum and urine. Tropical and Geographic Medicine 46, 233-238.
32. DEELDER, A. M., RUITENBERG, E. J., KORNELIS, D. & STEERENBERG, P. A. (1977). Schistosoma mansoni: Comparison of the immunoperoxidase techniques DASS and ELISA, for human diagnosis. Experimental Parasitology 41, 133-140.
33. DEELDER, A. M., VAN DAM, G. J., KORNELIS, D., FILLIE, Y. E. & VAN ZEYL, R. J. M. (1996). Schistosoma analysis of monoclonal-antibodies reactive with the circulating antigens CAA and CCA. Parasitology 112, 21-35.
34. DE JONGE, N., BOERMAN, O. C. & DEELDER, A. M. (1989). Time-resolved immunofluorometric assay (TR-IFMA) for the detection of schistosome circulating anodic antigen. Transactions of the Royal Society of Tropical Medicine and Hygiene 83, 659-663.
35. DE JONGE, N., FILLIE, Y. E. & DEELDER, A. M. (1987). A simple and rapid treatment (trichloracetic-acid precipitation) of serum samples to prevent non-specific reactions in the immunoassay of a proteoglycan. Journal of Immunological Methods 99, 195-197.
36. DE JONGE, N., GRYSEELS, B., HILBERATH, G. W., POLDERMAN, A. M. & DEELDER, A. M. (1988). Detection of circulating anodic antigen by ELISA for seroepidemiology of schistosomiasis mansoni. Transactions of the Royal Society of Tropical Medicine and Hygiene 82, 591-594.
37. DE JONGE, N., RABELLO, A. L. T., KRIJGER, F. W., KREMSNER, P. G., ROCHA, R. S., KATZ, N. & DEELDER, A. M. (1991). Levels of the schistosome circulating anodic and cathodic antigens in serum of schistosomiasis patients from Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene 85, 756-759.
38. DE JONGE, N., SCHOMMER, G., KRIJGER, F. W., FELDMIEIR, H., ZWINGENBERGER, K., STEINER, A., BIENZLE, U. & DEELDER, A. M. (1989). Presence of circulating anodic antigen in serum of Schistosoma intercalatum-infected patients from Gabon. Acta Tropica 46, 115-120.
39. DENNIS, R. D., BAUMEISTER, S., LAUER, G., RICHTER, R. & GEYER, E. (1996). Neutral glycolipids of Schistosoma mansoni as feasible antigens in the detection of schistosomiasis. Parasitology 112, 295-307.
40. DE NOYA, B. A., CESARI, I. M., LOSADA, S., COLMENARES, C., BALZAN, C., HOEBERE, J. & NOYA, O. (1997). Evaluation of alkaline phosphatase immunoassay and comparison with other diagnostic methods in areas of low transmission of schistosomiasis. Acta Tropica 66, 69-78.
41. DE VLAS, S. J. & GRYSEELS, B. (1992). Underestimation of Schistosoma mansoni prevalences. Parasitology Today 8, 274-277.
42. DE VLAS, S. J., GRYSEELS, B., VAN OORTMARSSEN, G. J., POLDERMAN, A. M. & HABBEMA, J. D. F. (1993). A pocket chart to estimate true Schistosoma mansoni prevalences. Parasitology Today 9, 305-307.
43. DISCH, J., GARCIA, M. M. A., KRIJGER, G. W., AMORIM, M. N., KATZ,N., DEELDER, A. M., GRYSEELS, B. & RABELLO, A. (1997). Daily fluctuations of levels of circulating cathodic antigen in urine of children infected with Schistosoma mansoni in Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene 91, 222-225.
44. DOENHOFF, M. J. (1998a). A role for granulomatous inflammation in the transmission of infections disease: schistosomiasis and tuberculosis. Parasitology 115, S113-S125.
45. DOENHOFF, M. J. (1998b). A vaccine for schistosomiasis: alternative approaches. Parasitology Today 14, 105-109.
46. DOENHOFF, M. J. (1998c). Is schistosomicidal chemotherapy sub-curative? Implications for drug resistance. Parasitology Today 14, 434-435.
47. DOENHOFF, M. J., BUTTERWORTH, A. E., HAYES, R. J., STURROCK, R. F., OUMA, J. H., KOECH, D., PRENTICE, M. & BAIN, J. (1993). Seroepidemiology and serodiagnosis of schistosomiasis in Kenya using crude and purified egg antigens of Schistosoma mansoni in ELISA. Transactions of the Royal Society of Tropical Medicine and Hygiene 87, 42-48.
48. DOENHOFF, .M. J., DUNNE, D. W. & LILLYWHITE, J. E. (1989). Serology of Schistosoma mansoni infections after chemotherapy. Transactions of the Royal Society of Tropical Medicine and Hygiene 83, 237-238.
49. DUNN, D. W., AGNEW, A. M., MODHA, J. & DOENHOFF, M. J. (1986). Schistosoma mansoni egg antigens: preparation of rabbit antisera with monospecific immunoprecipitating activity, and their use in antigen characterization. Parasite Immunology 8, 575-586.
50. DUNNE, D. W., BAIN, J., LILLYWHITE J. & DOENHOFF, M. J. (1984). The stage-, strain-, and species-specificity of a Schistosoma mansoni egg antigen fraction with serodiagnostic potential. Transactions of the Royal Society of Tropical Medicine and Hygiene 78, 460-470.
51. DUNNE, D. W., HILLYER, G. V. & VAZQUEZ, G. (1988). Schistosoma mansoni egg antigens (CEF6): immunoserology with oxaminiquine-treated patients and involvement of CEF6 in the circumoval precipitin reaction. American Journal of Tropical Medicine and Hygiene 38, 508-514.
52. DUNNE, D. W., JONES, F. M. & DOENHOFF, M. J. (1991). The purification, characterization, serological activity and hepatotoxic properties of two cationic glycoproteins (alpha-1 and omega-1) from Schistosoma mansoni eggs. Parasitology 103, 225-236.
53. DUNNE, D. W., LUCAS, S., BICKLE, Q., PEARSON, S., MADGWICK, L., BAIN, J. & DOENHOFF, M. J. (1981). Identification and partial purification of an antigen (omega 1) from Schistosoma mansoni eggs which is putatively hepatotoxic in T-cell deprived mice. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 54-71.
54. EBRAHIM, A., EL MORSHEDY, H., OMER, E., EL DALY, S. & BARAKAT, R. (1997). Evaluation of the Kato-Katz thick smear and formol ether sedimentation techniques for quantitative diagnosis ot Schistosoma mansoni infection. American Journal of Tropical Medicine and Hygiene 57, 706-708.
55. EL MORSHEDY, H., KINOSIEN, B., BARAKAT, R., OMER, E., KHAMIS, N., DEELDER, A. M. & PHILLIPS, M. (1996). Circulating anodic antigen for detection of Schistosoma mansoni infection in Egyptian patients, American Journal of Tropical Medicine and Hygiene 54, 149-153.
56. EL MISSIRY, A. G., EL SEROUGI, A. O., SALAMA, M. M. I. & KAMAL, A. M. (1990). Evaluation of the dot ELISA technique in the serodiagnosis of schistosomiasis in Egypt. Journal of the Egyptian Society of Parasitology 20, 639-645.
57. EL SAYED, L. H., GHONEIM, H., DEMIAN, S. R., EL SAYED, M. H., TAWFIK, N. M., SAKR, I., ABOU-BASHA, L., RENGANATHAN, E., KLINKERT, M. Q. & ABOURAWASH, N. (1998). Diagnostic significance of Schistosoma mansoni proteins Sm31 and Sm32 in human schistosomiasis in an endemic area in Egypt. Tropical Medicine and International Health 3, 721-727.
58. ELTIRO, F., YE-EBIYO, Y. & TAYLOR, M. G. (1992). Evaluation of an enzyme linked immunosorbent assay (ELISA) using Schistosoma mansoni soluble egg antigen as a diagnostic tool for Schistosoma mansoni infection in Ethiopian schoolchildren. Journal of Tropical Medicine and Hygiene 95, 52-56.
59. ENGELS, D., SINZINKAYO, E., DE VLAS, S. J. & GRYSEELS, B. (1997). Intraspecimen fecal egg count variation in Schistosoma mansoni infection. American Journal of Tropical Medicine and Hygiene 57, 571-577.
60. ENGELS, D., SINZINKAYO, E. & GRYSEELS, B. (1996). Day-to-day egg count fluctuation in Schistosoma mansoni infection and its operational implications. American Journal of Tropical Medicine and Hygiene 54, 319-324.
61. ENGVALL, E. & PERLMANN, P. (1971). ELISA: quantitative assay of immunoglobulin G. Immunochemistry 8, 871-874.
62. EVENGARD, B. (1990). Diagnostic and clinical aspects of schistosomiasis in 182 patients treated on a Swedish ward for tropical diseases during a 10 year period. Scandinavian Journal of Infectious Diseases 22, 585-591.
63. EVENGARD, B., HAMMARSTROM, L., SMITH, C. I. & LINDER, E. (1990). Early antibody responses in human schistosomiasis. Clinical and Experimental Immunology 80, 69-76.
64. EVENGARD, B., WOLOWCZUK, I., MARGUERITE, M., HAMMARSTROM, L., SMITH, E. & AURIAULT, C. (1994). IgG subclass-associated differences in anti-schistosomal antibody specificity. Scandinavian Journal of Immunology 40, 618-622.
65. FEINSTEIN, A. R. (1975). Clinical biostatistics. XXXI: On the sensitivity, specificity and discrimination of diagnostic tests. Clinical and Pharmacological Therapeutics 17, 104-116.
66. FELDMIEIR, H. & POGGENSEE, G. (1993). Diagnostic techniques in schistosomiasis control. A review. Acta Tropica 52, 205-220.
67. FERRARI, T. C. A., MOREIRA, P. R. R., OLIVEIRA, R. C., FERRARI, M. L. A., GAZZINELLI, G. & CUNHA, A. S. (1995). The value of an enzyme-linked immunosorbent assay for the diagnosis of schistosomiasis mansoni myeloradiculopathy. Transactions of the Royal Society of Tropical Medicine and Hygiene 89, 496-500.
68. FLETCHER, R. H., FLETCHER, A. W. & WAGNER, E. H. (1996). Clinical Epidemiology: The Essentials. (3rd Ed) Baltimore, Williams & Wilkins.
69. GAZZINELLI, G., LAMBERTUCCI, J. R., KATZ, N., ROCHA, R. S., LIMA, M. S., & COLLEY, D. G. (1985). Immune responses during human schistosomiasis mansoni. XI: Immunologic status of patients with acute infections and after treatment. Journal of Immunology 135, 2121-2127.
70. CHANDOUR, A. M., TRICKER, K., DOENHOFF, M. J., ALROBAT, A. A. & BANAJA, A. A. (1947). An enzyme-linked immunosorbent assay using Schistosoma mansoni purified egg antigen for the diagnosis of schistosomiasis in Saudi Arabia. Transactions of the Royal Society of Tropical Medicine and Hygiene 91, 287-289.
71. GROCAN, J, L., KREMSNER, P. G., VAN DAM, G. J., DEELDER, A. M. & YAZDANBAKSH, M. (1997a). Anti-schistosome IgG4 and IgG at 2 years after chemotherapy: infected versus uninfected individuals. Journal of Infectious Diseases 176, 1344-1350.
72. GROGAN, J. L., KREMSNER, P. G., VAN DAM, G. J., METZGER, W., MORDMULLER, B., DEELDER, A. M. & YAZDANBAKSH, M. (1996). Antischistosome IgG4 and IgE responses are affected differentially by chemotherapy in children versus adults. Journal of Infectious Diseases 173, 1242-1247.
73. GROGAN, J., ROTMANS, P., GHONHEIM, H., DEELDER, A. M., YAZDANBAKSH, M. & KLINKERT, M. Q. (1997b). Recognition of recombinant Schistosoma mansoni cathepsins B and L by human IgG1 and IgG4 antibodies. Parasite Immunology 19, 215-220.
74. GRYSEELS, B., STELMA, F. F., TALLA, I., VAN DAM, G. J., POLMAN, K., SOW, S., DIAW, M., STURROCK, R. F., DOENHRINGSCHWERDTFEGER, E., KARDORRFF, R., DECAM, C., NIANG, M. & DEELDER, A. M. (1994). Epidemiology, immunology and chemotherapy of Schistosoma mansoni infections in a recently exposed community in Senegal. Tropical and Geographical Medicine 46, 209-219.
75. GRYSEELS, B. & DE VLAS, S. J. (1996). Worm burdens in schistosome infections. Parasitology Today 12, 115-119.
76. GRZYCH, J.-M., DISSOUS, C., CAPRON, M., TORRES, S., LAMBERT, P.-H. & CAPRON, A. (1987). Schistosoma mansoni shares a protective carbohydrate epitope with keyhole limpet haemocyanin. Journal of Experimental Medicine 165, 865-878.
77. GUNDERSON, S. G., HAAGENSEN, I., JONASSEN, T. O., FIGENSCHAU, K. J., DE JONGE, N. & DEELDER, A. M. (1992). Magnetic bead antigen capture enzyme-linked immunoassay in microtitre trays for rapid detection of schistosomal circulating anodic antigen. Journal of Immunological Methods 148, 1-8.
78. HAMBURGER, J., PELLEY, R. P. & WARREN, K. S. (1976). Schistosoma mansoni soluble egg antigens: determination of the stage and species specificity of their serologic reactivity by radioimmunoassay. Journal of Immunology 117, 1561-1566.
79. HAMILTON, J. V., CHIODINI, P. L., FALLON, P. G. & DOENHOFF, M. J. (1999). Periodate-sensitive immunological cross-reactivity between keyhole limpet haemocyanin (KLII) and serodiagnostic Schistosoma mansoni egg antigens. Parasitology 118, 83-89.
80. HANCOCK, K., MOHAMED, Y. B., XUE, H. C., NOH, J., DOTSON, E. M. & TSANG, V. C. W. (1997). A recombinant protein from Schistosoma mansoni useful for the detection of S. mansoni and Schistosoma haematobitium antibodies. Journal of Parasitology 83, 612-618.
81. HANCOCK, K. & TSANG, V. C. W. (1986). Development and optimisation of the FAST-ELISA for detecting antibodies to Schistosoma mansoni. Journal of Immunological Methods 92, 167-176.
82. HASSAN, F., ABDEL-WAHAB, M. F., NOSSEUR, A., SEDEEKT, S., SHEHATA, A. & MASOOD, M. A. (1979). Evaluation of the enzyme-linked immunosorbent assay in the immunodiagnosis of schistosomiasis. Journal of Tropical Medicine and Hygiene 82, 3-7.
83. HASSAN, M. M., BADAWI, M. A. & STRAND, M. (1992). Circulating schistosomal antigen in diagnosis and assessment of cure in individuals infected with Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 46, 737-744.
84. HILLYER, G. V. & PELLEY, R. P. (1980). The major serological antigen (MSA1) from Schistosoma mansoni eggs is a circumoval precipitin. American Journal of Tropical Medicine and Hygiene 29, 382-585.
85. HULDT, G., LAGERQUIST, B., PHILLIPS, T., DRAPER, C. C. & VOLLER, A. (1975). Detection of antibodies in schistosomiasis by enzyme-linked immunosorbent assay (ELISA). Annals of Tropical Medicine and Parasitology 69, 483-488.
86. IDRIS, M. A. & RUPPEL, A. (1988). Diagnostic Mr 31/32000 Schistosoma mansoni proteins (Sm31/32): Reaction with sera from Sudanese patients infected with S. mansoni or S. haematobium. Journal of Helminthology 62, 95-101.
87. ISMAIL, M. M., BRUCE, J. I., ATTIA, M., TAYEL, S. E., SABAH, A. A. & EL-AHMEDAWY, B. A. (1989). The detection of IgR by radio-allergosorbent technique (RAST) and ELISA in Egyptian cases of schistosomiasis. Journal of the Egyptian Society of Parasitology 19, 29-34.
88. JAMALY, S., CHIHANI, T., DEELDER, A. M., GABONE, R., NILSSON, L. A. & OUCHTERLONY, O. (1997). Polypropylene fibre web, a new matrix for sampling blood for immunodiagnosis of schistosomiasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 91, 412-115.
89. JEMANEH, L. (1993). Comparison of immunodiagnosis (ELISA) and stool examination (Kato technique) in the diagnosis of Schistosoma mansoni in Ethiopia. Ethiopian Medical Journal 31, 37-49.
90. KAGAN, I. (1968). A critical review of immunological methods for the diagnosis of Bilharziasis. Bulletin of the World Health Organisation 25, 611-674.
91. KAMAL, K. A., SHAHEEN, H. I. & EL SAID, A. A. (1994). Applicability of ELISA on buffer-cluates of capillary blood spotted on filter papers for the diagnosis and clinical staging of human schistosomiasis. Tropical and Geographical Medicine 46, 138-141.
92. KARANJA, D. M. S., COLLEY, D. G., NAHLEN, B. L., OUMA, J. H. & SECOR, W. E. (1997). Studies on schistosomiasis in Western Kenya. I. Evidence for immune facilitated excretions of schistosome eggs from patients with Schistosoma mansoni and Human Immunodeficiency Virus co-infections. American Journal of Tropical Medicine and Hygiene 56, 515-521.
93. KATO, K. & MIURA, N. (1954). Comparative examinations. Japanese Journal of Parasitology 3, 35-37.
94. KATZ, N., CHAVES, A. & PELLEGRINO, J. (1972). A simple device for quantitative stool thick smear technique in schistosomiasis mansoni. Revista do Instituto de Medicina Tropical Sao Paulo 14, 397-400.
95. KHALIL, H. M., MAKLED, M. K. I., EL-MISSIRY, A. G., KHALIL, N. M. & SONBOL, S. E. (1989). The application of Schistosoma mansoni adult and soluble egg antigens for the serodiagnosis of schistosomiasis by CIEP, IHA and ELISA. Journal of the Egyptian Society of Parasitology 19, Suppl. 2, 827-843.
96. KLINKERT, M. Q., BOMMERT, K., MOSER, D., FELLEISEN, R., LINK, G., DOUMBO, O. & BECK, E. (1991). Immunological analysis of cloned schistosomiasis mansoni antigens Sm31 and Sm32 with sera of schistosomiasis patients. Tropical and Medical Parasitology 42, 319-324.
97. KLINKERT, M. Q., RUPPEL, A. & BECK, E. (1987). Cloning of diagnostic 31/32 kDa antigens of Schistosoma mansoni. Molecular and Biochemical Parasitology 25, 247-255.
98. KNIGHT, W. B., HIATT, R. A., CLINE, B. L. & RITCHIE, L. S. (1976). A modification of the formol-ether concentration technique for increased sensitivity in detecting Schistosoma mansoni eggs. American Journal of Tropical Medicine and Hygiene 25, 818-823.
99. KRIJGER, E. W., VAN LIESHOUT, L. & DEELDER, A. M. (1994). A simple technique to pretreat urine and serum samples for quantitation of schistosome circulating anodic and cathodic antigen. Acta Tropica 56, 55-63.
100. LANGLEY, G., KARIUKI, H. C., HAMMERSLEY, A. P., OUMA, J. H., BUTTERWORTH, A. E. & DUNNE, D. W. (1994). Human IgG subclass responses and subclass restriction to Schistosoma mansoni egg antigens. Immunology 83, 651-658.
101. LI, Y. L., IDRIS, M. A., CORACHAN, M., HAN, J. J., KIRSCHIFINK, M. & RUPPEL, A. (1996). Circulating antigens in schistosomiasis: detection of 31/32-kDa proteins in sera from patients infected with Schistosoma japonicum, S. mansoni, S. haematobium, or S. intercalatum. Parasitology Research 82, 14-18.
102. LI, Y. L., SONG, W. J., HAN, J. J. & RUPPEL, A. (1994). Detection of Schistosoma japonicum antigen (Sj31/32) in sera of Chinese patients using a sandwich ELISA based on a monoclonal antibody. Tropical Medicine and Parasitology 45, 115-118.
103. LI, Y. S., ROSS, A. G. P., LI, Y., HE, Y. K., LUO, X. S. & McMANUS, D. P. (1997). Serological diagnosis of Schistosoma japonicum infections in China. Transactions of the Royal Society of Tropical Medicine and Hygiene 91, 19-21.
104. LI, Z., KING, C. L., OGUNDIPE, J. O., LICATE, L. S. & BLANTON, R. E. (1995). Preferential recognition by human IgE and IgG4 of a species specific Schistosoma haematobium serine protease inhibitor. Journal of Infectious Diseases 171, 416-422.
105. LIPPS, G., FULLKRUG, R. & BECK, E. (1996). Cathepsin-B of Schistosoma mansoni - purification and activation of the recombinant proenzyme by Saccharomyces cerevisiae. Journal of Biological Chemistry 271, 1717-1725.
106. LIVINGSTON, P. O. (1995). Approaches to augmenting the immunogenicity of melanoma gangliosides: from whole melanoma cells to ganglioside-KLH conjugate vaccines. Immunological Reviews 145, 147-166.
107. LUNDE, M. N. & OTTESEN, E. A. (1980). Enzyme-linked immunosorbent assay (ELISA) for detecting IgM and IgE antibodies in human schistosomiasis. American Journal of Tropical Medicine and Hygiene 29, 82-85.
108. LUNDE, M. N., OTTESEN, E. A. & CHEEVER, A. W. (1979). Serological differences between acute and chronic schistosomiasis mansoni detected by enzyme-linked immunosorbent assay. American Journal of Tropical Medicine and Hygiene 28, 87-91.
109. MADDISON, S. E. (1987). The present status of serodiagnosis and seroepidemiology of schistosomiasis. Diagnostic Microbiology and Infectious Disease 7, 93-105.
110. MADDISON, S. K. (1991). Serodiagnosis of parasitic diseases. Clinical Microbiology Reviews 4, 457-469.
111. MADDISON, S. E., SLEMENDA, S. B., TSANG, V. C. & POLLARD, R. A. (1985). Serodiagnosis of Schistosoma mansoni with adult worm antigen in an enzyme-linked immunosorbent assay using a standard curve developed with a reference serum pool. American Journal of Tropical Medicine and Hygiene 34, 484-494.
112. MANSOUR, M. M., OMER-ALI, P., FARID, Z., SIMPSON, A. J. G. & WOODY, J. (1989). Serological differentiation of acute and chronic schistosomiasis mansoni by antibody responses to keyhole limpet haemocyanin. American Journal of Tropical Medicine and Hygiene 41, 338-344.
113. MARTIN, L. K. & BEAVER, P, C. (1968). Evaluation of Kato thick-smear technique for quantitative diagnosis of helminth infections. American Journal of Tropical Medicine and Hygiene 17, 382-391.
114. MARKL, J., NOUR EL DIN, M., WINTER-SIMANOWSKI, S. & SIMANOWSKI, U. A. (1991). Specific IgG activity of sera from Egyptian schistosomiasis patients to keyhole limpet haemocyanin (KLH). Naturwissenschaften 78, 30-31.
115. McLAREN, M. L., DRAPER, C. C., ROBERTS, J. M., MINTER-GOEDBLOED, E., LIGHTHART, G. S., TEESDALE, C. H., AMIN, M. A., OMER, A. H. S., BARTLETT, A. & VOLLER, A. (1978). Studies on the enzyme linked immunosorbent assay (ELISA) test for Schistosoma mansoni infections. Annals of Tropical Medical Parasitology 72, 243-253.
116. McLAREN, M. L., LILLYWHITE, J. E., DUNNE, D. W. & DOENHOFF, M. J. (1981). Serodiagnosis of human Schistosoma mansoni infections: enhanced sensitivity and specificity in egg antigen omega 1 and alpha 1. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 72-79.
117. MERKELBACH, A., HASSE, S., DELL, R., ESCHBECK, A. & RUPPEL, A. (1994). cDNA sequences of Schistosoma japonicum coding for two cathepsin-like proteins and Sj32. Tropical Medicine and Parasitology 45, 193-198.
118. MOSER, D., DOENHOFF, M. J. & KLINKERT, M. Q. (1992). A stage-specific calcium-binding protein expressed in eggs of Schistosoma mansoni. Molecular and Biochemical Parasitology 51, 229-238.
119. MOSEr, D., DOUMBO, O. & KLINKERT, M.-Q. (1990). The humoral response to heat shock protein 70 in human and murine schistosomiasis mansoni. Parasite Immunology 12, 341-342.
120. MOTT, K. E. & DIXON, H. (1982). Collaborative study on antigens for immunodiagnosis of schistosomiasis. Bulletin of the World Health Organisation 60, 233-244.
121. MOTT, K. E., DIXON, H., CARTER, C. E., GARCIA, E., ISHII, A., MATSUDA, H., MITCHELL, G., OWHASHI, M., TANAKA, H. & TSANG, V. C. (1987). Collaborative study on antigens for the immunodiagnosis of Schistosoma japonicum infection. Bulletin of the World Health Organisation 65, 233-244.
122. NASH, T. E., GARCIACOYCO, C., RUTZTIBEN, E., NAZARIOLOPEZ, H. A., VAZQUEZ, G. & TORRESBORGES, A. (1983). Differentiation of acute and chronic schistosomiasis by antibody responses to specific schistosome antigens. American Journal of Tropical Medicine and Hygiene 32, 776-784.
123. NASH, T. E., LUNDE, M. N. & CHEEVER, A. W. (1981). Analysis and antigenic activity of a carbohydrate fraction derived from adult Schistosoma mansoni. Journal of Immunology 126, 805-810.
124. NASH, T. E., PRESCOTT, B. & NEVA, F. A. (1974). The characteristics of a circulating antigen in schistosomiasis. Journal of Immunology 112, 1500-1507.
125. NEWPORT, G. R., McKERROW, J. H., HEDSTROM, R. C., KALLESTAD, J., TARR, P., KLEBANOFF, S. & AGABIAN, N. (1988). Identification, molecular cloning and expression of a schistosome antigen displaying diagnostic potential. American Journal of Tropical Medicine and Hygiene 38, 540-546.
126. NIBBELING, H. A. M., VAN ETTEN, L., FILLIE, Y. E. & DEELDER, A. M. (1997). Enhanced detection of Schistosoma circulating antigens by testing 1 ml urine samples using immunomagnetic beads. Acta Tropica 66, 85-92.
127. OKABE, K. & TANAKA, T. (1961). Urine precipitation reaction for schistosomiasis japonica. Kurume Medical Journal 8, 24-29.
128. PELLEY, R. P, WARREN, K. S. & JORDAN, P. (1977). Purified antigen radioimmunoassay in serological diagnosis of schistosomiasis mansoni. Lancet ii, 781-785.
129. PETERS, P. A., MAHMOUD, A. A. F., WARREN, K. S., OUMAN, J. H. & ARAP SIONGOK, T. K. (1976). Field studies of a rapid, accurate means of quantifying Schistosoma haematobium eggs in urine samples. Bulletin of the World Health Organisation 54, 159-162.
130. PINO-HEISS, S., PETITT, M., BECKSTEAD, J. H. & McKERROW, J. H. (1986). Preparation of monoclonal antibodies and evidence for a host immune response to the preacetabular gland proteinase of Schistosoma mansoni cercariae. American Journal of Tropical Medicine and Hygiene 35, 536-543.
131. POJOL, F. H. & CESARI, I. M. (1990). Antigenicity of adult S. mansoni alkaline phosphatase. Parasite Immunology 12, 189-198.
132. POJOL, F. H., DE NOYA, B. A. & CESARI, I. M. (1989). Immunodiagnosis of schistosomiasis mansoni with APIA (alkaline phosphatase immunoassay). Immunological Investigations 18, 1071-1080.
133. RABELLO, A. (1997). Diagnosing schistosomiasis. Memorias do Instituto Oswaldo Cruz 92, 669-676.
134. RABELLO, A. L. T., GARCIA, M. M. A., DA SILVA, R. A. P., ROCHA, R. S. & KATZ, N. (1997). Humoral immune responses in patients with acute Schistosoma mansoni infection who were followed up two years after treatment. Clinical Infectious Diseases 24, 304-308.
135. RABELLO, A. L. T., GARCIA, M. M. A., NETO, E. D., ROCHA, R. S. & KATZ, N. (1993). Dot-dye-immunoassay and dot-ELISA for the serological differentiation of acute and chronic schistosomiasis-mansoni using keyhole limpet haemocyanin as antigen. Transactions of the Royal Society of Tropical Medicine and Hygiene 87, 279-281.
136. RABELLO, A. L. T., NETO, E. D., GARCIA, M. M. A. & KATZ, N. (1992). Dot-dye immunoassay for the diagnosis of schistosomiasis mansoni. Memorias do Instituto Oswaldo Cruz 87, 187-190.
137. RAMZY, R. M. R., FARIS, R., BAHGAT, M., HELMY, H., FRANKLIN, C. & McKERROW, J. H. (1097). Evaluation of a stage-specific proteolytic enzyme of Schistosoma mansoni as a marker of exposure. American Journal of Tropical Medicine and Hygiene 56, 668-673.
138. RIDLEY, D. S. & HAWGOOD, B. C. (1956). The value of formal ether concentration of fecal cysts and ova. Journal of Clinical Pathology 9, 74-76.
139. RUIZ-TIBEN, E., HILLYER, G. V., KNIGHT, W. B., GOMEZ DE RIOS, I. & WOODALL, J. P. (1979). Intensity of infection with Schistosoma mansoni: its relationship to the sensitivity and specificity of serologic tests. American Journal of Tropical Medifine and Hygiene 28, 230-236.
140. RUPPEL, A., DIESFELD, H. J. & ROTHER, U. (1985). Immunoblot analysis of Schistosoma mansoni antigens with sera of schistosomiasis patients: diagnostic potential of an adult schistosome polypeptide. Clinical and Experimental Immunology 62, 499-506.
141. RUPPEL, A., IDRIS, M. A., SULAIMAN, S. M. & HILALI, A. M. H. (1990). Schistosoma mansoni diagnostic antigens (Sm31/32): a sero-epidemiological study in the Sudan. Tropical Medicine and Parasitology 41, 127-130.
142. RUPPEL, A., SHI, Y. E., WEI, D. X. & DIESFELD, H. J. (1987). Sera of Schistosoma japonicum infected patients cross-react with diagnostic 31/32 kDa proteins of Schistosoma mansoni. Clinical and Experimental Immunology 69, 291-298.
143. SANTORO, F. Y., CARLIER, Y., BOROJEVIC, R., BOUT, D., TACHON, P. & CAPRON, A. (1977). Parasite M antigen in milk from mothers infected with Schistosoma mansoni. Annals of Tropical Medicine and Parasitology 71, 121-123.
144. SATTI, M. Z., LIND, P., VENNERVALD, B. J., SULAIMAN, S. M., DAFFALLA, A. A. & GHALIB, H. W. (1996a). Specific immumoglobulin in measurements related to exposure and resistance to Schistosoma mansoni infection in Sudanese canal cleaners. Clinical and Experimental Immunology 106, 45-54.
145. SATTI, M. Z., EBBESEN, F., VENNERVALD, B., LIND, P., GHALIB, H., SULAIMAN, S., DAFFALLA, A. & SKOV, P. S. (1996b). Use of a new glass microfibre histamine-release method to study the modulation of the host response in human schistosomiasis mansoni -individuals with different degrees of exposure to the disease show differing antibody biological function. Tropical Medicine and International Health 1, 655-666.
146. SLEIGH, A., HOFF, R., MOTT, K., BARRETO, M., DE. PALVA, T. M., PEDROSA, J. D. S. & SHERLOCK, I. (1982). Comparison of filtration staining (Bell) and thick smear (Kato) for the detection and quantitation of Schistosoma mansoni eggs in faeces. Transactions of the Royal Society of Tropical Medicine and Hygiene 76, 403-406.
147. TANABE, M., OKAZAKI, M., KOBAYASHI, S., KANEKO, N., SEKIGUCHI, T., TATENO, S., MOTTA, S. R. N. & TAKEUCHI, T. (1990). Serological studies on schistosomiasis mansoni in the Northeast Brazil. Revista do Instituto de Medicina Tropical Sao Paulo 32, 121-131.
148. TOSSWILL, J. H. C. & RIDLEY, D. S. (1986). An evaluation of the ELISA for schistosomiasis in a hospital population. Transactions of the Royal Society of Tropical Medicine and Hygiene 80, 435-441.
149. TOY, L., PETIT, M., WANG, Y. F., HEDSTROM, R. & McKERROW, J. H. (1987). The immune response to stage-specific proteolytic enzymes of Schistosoma mansoni. In Molecular Paradigms for Eradicating Helminthic Parasites (ed. Agabian, N., Goddman, H. & Nogueira, N.) pp. 85-103. Alan R. Liss Inc., New York, USA.
150. TSANG, V. C. W., HANCOCK, K., KELLEY, M. A., WILSON, B. C. & MADDISON, S. E. (1983a). Schistosoma mansoni adult microsomal antigens, a serologic reagent. 2. Specificity of antibody responses to the S. mansoni microsomal antigen. Journal of Immunology 130, 1366-1370.
151. TSANG, V. C. W., TAO, Y. X., QUI, L. S. & XUE, H. C. (1982). Fractionation and quantitation of egg antigens from Schistosoma japonicum by the single-tube kinetic-dependent enzyme-linked immunosorbent-assay (k-ELISA) higher antigenic activity in urea-soluble than in aqueous-soluble fractions. Journal of Parasitology 68, 1034-1043.
152. TSANG, V. C. W., TSANG, K. R., HANCOCK, K., KELLEY, M. A., WILSON, B. U. & MADDISON, S. E. (1983b). Schistosoma mansoni adult microsomal antigens, a serologic reagent. 1. Systematic fractionation, quantitation, and characterization of antigenic components. Journal of Immunology 130, 1359-1365.
153. TSANG, V. C. W. & WILKINS, P. P. (1997). Immunodiagnosis of schistosomiasis. Immunological Investigations 26, 175-188.
154. VALLI, L. C. P., KANAMURA, H. Y., DA SILVA, R. M., SILVA, M. I. P. G., VELLOSA, S. A. G. & GARCIA, E. T. (1997). Efficacy of an enzyme-linked immunosorbent assay in the diagnosis of and serologic distinction between acute and chronic Schistosoma mansoni infection. American Journal of Tropical Medicine and Hygiene 57, 358-362.
155. VAN DAM, G. J., BERGWERF, A. A., THOMASOATES, J. E., ROTMANS, J. P., KAMERLING, J. P., FLIEGENTHART, J. F. G. & DEELDER, A. M. (1994). The immunologically reactive O-linked polysaccharide chains derived from circulating cathodic antigen isolated from the human blood Huke Schistosoma mansoni have Lewis-X as repeating unit. European Journal of Biochemistry 225, 467-482.
156. VAN DAM, G. J., CLAAS, F. H. J., YAZDANBAKHSH, M., KRUIZE, Y. C. M., VAN KEULEN, A. C. I., FERREIRA, S. T. M. F., ROTMANS, J. P. & DEELDER, A. M. (1996). Schistosomas mansoni excretory circulating cathodic antigen shares Lewis-x epitopes with a human granulocyte surface antigen and evokes host antibodies mediating complement-dependent lysis of granulocytes. Blood 88, 4246-4251.
157. VAN DAM, G. J., STELMA, F. F., GRYSEELS, B., FERREIRA, S. T. M. F., TALLA, I., NIANG, M., ROTMANS, J. P. & DEELDER, A. M. (1996). Antibody response patterns against Schistosoma mansoni in a recently exposed community in Senegal. Journal of Infectious Diseases 173, 1232-1241.
158. VAN ETTEN, L., ENGELS, D., KRIJGER, F. W., NKULIKYINKA, L., GRYSEELS, B. & DEELDER, A. M. (1996). Fluctuation of schistosome circulating antigen levels in urine of individuals with Schistosoma mansoni infection in Burundi. American Journal of Tropical Medicine and Hygiene 54, 348-351.
159. VAN ETTEN, L., KREMSNER, P. G., KRIJGER, F. W. & DEELDER, A. M. (1997a). Day-to-day variation of egg output and schistosome circulating antigens in urine of Schistosoma haematobium-infected school children from Gabon and follow-up after chemotherapy. American Journal of Tropical Medicine and Hygiene 57, 337-341.
160. VAN ETTEN, L., VAN LIESHOUT, L., MANSOUR, M. M. & DEELDER, A. M. (1997b). A reagent strip antigen capture assay for the assessment of cure of schistosomiasis patients. Transactions of the Royal Society of Tropical Medicine and Hygiene 91, 154-155.
161. VAN LIESHOUT, L., DE JONGE, N., EL MASRY, N. A., MANSOUR, M. M., BASSILY, S., KRIJGER, F. W. & DEELDER, A. M. (1994). Monitoring the efficacy of different doses of praziquantel by quantification of circulating antigens in serum and urine of schistosomiasis patients. Parasitology 108, 519-526.
162. VAN LIESHOUT, L., DE JONGE, N., EL MASRY, N. A., MANSOUR, M. M., KRIJGER, F. W. & DEELDER, A. M. (1992). Improved diagnostic performance of the circulating antigen assay in human schistosomiasis by parallel testing for circulating anodic and cathodic antigens in serum and urine. American Journal of Tropical Medicine and Hygiene 47, 463-169.
163. VAN; LIESHOUT, L., PANDAY, U. G., DE JONGE, N., KRIJGER, F. W., OOSTBURG, B. F. J., POLDERMAN, A. M. & DEELDER, A. M. (1995a). Immunodiagnosis of schistosomiasis mansoni in a low endemic area in Surinam by determination of the circulating antigens CAA and CCA. Acta Tropica 59, 19-29.
164. VAN LIESHOUT, L., POLDERMAN, A. M., DE VLAS, S. J., DE CALUWE, P., KRIJGER, F. W., GRYSEELS, B. & DEELDER, A. M. (1995b). Analysis of worm burden variation in human Schistosoma mansoni infections by determination of serum levels of circulating anodic antigen and circulating cathodic antigen. Journal of Infectious Diseases 172, 1336-1342.
165. VAN LIESHOUT, L., POLDERMAN, A. M., VISSER, L. G., VERWAY, J. J. & DEELDER, A. M. (1997). Detection of the circulating antigens CAA and CCA in a group of Dutch travellers with acute schistosomiasis. Tropical Medicine and International Health 2, 551-557.
166. VAN LIESHOUT, L., POLMAN, K., GRYSEELS, B. & DEELDER, A. M. (1998). Circulating anodic antigen levels in two areas endemic for schistosomiasis mansoni indicate differences in worm fecundity. Transactions of the Royal Society of Tropical Medicine and Hygiene 92, 115-119.
167. VAN'T WOUT, A. B., DE JONGE, N., WOOD, S. M., VAN LIESHOUT, L., MITCHELL, G. F. & DEELDER, A. M. (1995). Serum levels of circulating anodic antigen and circulating cathodic antigen detected in mice infected with Schistosoma japonicum or S. mansoni. Parasitology Research 81, 434-437.
168. VENKATESAN, P. & WAKELIN, D. (1993). ELISAs for parasitologists: or lies, damned lies and ELISAs. Parasitology Today 9, 228-232.
169. VERWEIJ, J. J., POLDERMAN, A. M., VISSER, L. G. & DEELDER, A. M. (1995). Measurement of antibody response to keyhole limpet haemocyanin was not adequate for early diagnosis of schistosomiasis in a group of Dutch visitors to Mali. Transactions of the Royal Society of Tropical Medicine and Hygiene 89, 48-50.
170. VISSER, L. G., POLDERMAN, A. M. & STUIVER, P. C. (1995). Outbreak of schistosomiasis among travellers returning from Mali, West Africa. Clinical Infectious Diseases 20, 280-285.
171. VOLLER, A., BARTLETT, A. & BIDWELL, D. E. (1976). Enzyme immunoassays for parasitic diseases. Transactions of the Royal Society for Tropical Medicine and Hygiene 70, 98-106.
172. VOLLER, A., BIDWELL, D. E., HULDT, G. & ENGVALL, E. (1974). A microplate method of ELISA and its application to malaria. Bulletin of the World Health Organization 51, 209-211.
173. WANG, S., ZENG, X. & YI, X. (1995). Purification of 31/32 kDa proteins of Schistosoma japonicum as antigens (Sj31/32) for ELISA and IHA. Chung-Kuo-Chi-Sheng-Chung-Hsueh-Yu-Chi-Sheng-Chung-Ping-Tsa-Chih 13, 25-31.
174. WARREN, K. S. (1973). The pathology of schistosome infections. Helminthological Abstracts 42, 591-593.
175. XU, H., REKOSH, D. M., ANDREWS, W., HIASHI, G. I., NICHOLSON, L. & LOVERDE, P. (1991). Schistosomiasis mansoni tropomyosin: production and purification of the recombinant protein and its immunodiagnostic potential. American Journal of Tropical Medicine and Hygiene 45, 121-131.
176. XU, C. G., TAYLOR, M. G., BICKLE, Q. D., SAVIOLI, L. & RENGANATHAN, E. A. (1993). Diagnosis of Schistosoma haematobium infection: evaluation of ELISA using a keyhole limpet haemocyanin or soluble egg antigen in comparison with detection of eggs of haematuria. Transactions of the Royal Society of Tropical Medicine and Hygiene 87, 654-658.
177. YACOUB, A. A. H. & LILLYWHITE, J. E. (1985). The effect of chemotherapy on the serological response of patients with schistosomiasis haematobium infection using the enzyme-linked immunosorbent assay. Journal of the Faculty of Medicine Baghdad 27, 19-29.
178. YE, X. P., DONNELLY, C. A., ANDERSON, R. M., FU, Y. L. & AGNEW, A. M. (1998). The distribution of Schistosoma japonicum eggs in faeces and the effect of stirring faecal specimens. Annals of Tropical Medicine and Parasitology 92, 181-185.
179. YUESHENG, L., RABELLO, A. L. T., SIMPSON, A. J. G. & KATZ, N. (1994). The serological differentiation of acute and chronic Schistosoma japonicum infection by ELISA using keyhole limpet haemocyanin as antigen. Transactions of the Royal Society of Tropical Medicine and Hygiene 88, 249-251.
180. ZHENG, X. F., WANG, S. P., LA, W. Z., XIANG, Y. D. & YI, X. Y. (1992). Studies on the KLH-ELISA and KLH-IHA for the diagnosis of Schistosoma japonicum infection. Chinese Journal of Schistosomiasis Control 4, 57-58.