Cathepsin B of Schistosoma mansoni: Purification and activation of the recombinant proenzyme secreted by Saccharomyces cerevisiae

Journal of Biological Chemistry

Volumn 271, Issue 3, 1996, Pages 1717-1725

  Lipps, Georg ^a,b   Füllkrug, Ralf ^a   Beck, Ewald ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

^b ScheBo Tech   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANILIDE; BENZYLOXYCARBONYLPHENYLALANYLARGININE 4 METHYL 7 COUMARYLAMIDE; BENZYLSULFONYL FLUORIDE; BESTATIN; CATHEPSIN B; COUMARIN DERIVATIVE; CYSTEINE PROTEINASE INHIBITOR; ENZYME PRECURSOR; HEMOGLOBIN; N BENZYLOXYCARBONYLARGINYLARGININE 4 METHYL 7 COUMARYLAMIDE; N BENZYLOXYCARBONYLARGINYLARGININE 4 NITROANILIDE; PEPSIN A; PEPSTATIN; RECOMBINANT PROTEIN; SERINE PROTEINASE INHIBITOR; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROCESSING; SACCHAROMYCES CEREVISIAE; SCHISTOSOMA MANSONI;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CATHEPSIN B; CLONING, MOLECULAR; DNA PRIMERS; ENZYME ACTIVATION; ENZYME PRECURSORS; HEMOGLOBINS; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPSIN A; PLASMIDS; PROTEASE INHIBITORS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RATS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SCHISTOSOMA MANSONI; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

MAMMALIA; SACCHAROMYCES CEREVISIAE; SCHISTOSOMA MANSONI; TREMATODA;

EID: 0030071561     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.271.3.1717     Document Type: Article

References (55)

1. McKerrow, J H (1989) Exp. Parasitol. 68, 111-115
2. Karrer, M K., P., Stacia, L & DiTomas, M E. (1993) Proc. Natl. Acad. Sci. U S. A. 90, 3063-3067
3. Keppler, D., Abramanson, M. & Sordat, B (1994) Biochem. Soc Trans. 22, 43-49
4. Pratt, D., Cox, G N , Milhausen, M. J. & Boisvenue, R. J (1990) Mol. Biochem. Parasitol 43, 181-192
5. Pratt, D., Boisvenue, R J. & Cox, G. N. (1992) Mol. Biochem. Parasitol 56, 39-48
6. Klinkert, M-Q., Ruppel, A. & Beck, E. (1987) Mol. Biochem. Parasitol. 25, 247-255
7. Ray, C. & McKerrow, J H (1992) Mol Biochem. Parasitol 51, 239-250
8. Chappell, C. L. & Dresden, M H. (1987) Arch. Biochem Biophys. 256, 560-568
9. Timms, A R. & Bueding, E (1959) Br. J. Pharmacol. 14, 68-73
10. Lindquist, R N , Senft, A. W , Petitt, M. & McKerrow, J. (1986) Exp. Parasitol 61, 398-404
11. Dresden, M. H. & Deelder, A. M (1979) Exp. Parasitol. 48, 190-197
12. Chappell, C. L. & Dresden, M H. (1986) Exp. Parasitol. 61, 160-167
13. Klinkert, M.-Q., Bommert, K., Moser, D., Felleisen, R., Link, G., Doumbo, O. & Beck, E. (1991) Trop. Med. Parasitol 42, 319-324
14. Klinkert, M.-Q., Ruppel, A., Felleisen, R, Link, G. & Beck, E. (1988) Mol. Biochem. Parasitol 27, 233-240
15. Gotz, B. & Klinkert, M -Q. (1993) Biochem. J 290, 801-806
16. Sambrook, J , Frisch, E. F. & Maniatis, T (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring, NY
17. Felleisen, R. & Klinkert, M -Q (1990) EMBO J. 9, 371-377
18. Baldari, C., Murray, J. A. H., Ghiara, P., Cesareni, G. & Galeotti, C. L. (1987) EMBO J. 6, 229-234
19. Guarante, L , Yocum, R. R. & Gifford, P. (1982) Proc. Natl. Acad. Sci. U S A. 79, 7410-7414
20. Dohmen, R J., Strasser, A. W. M., Honer, C. B. & Hollenberg, C. P. (1991) Yeast 7, 691-692
21. Laemmli, U K. (1970) Nature 227, 680-685
22. Schagger, H & von Jagow, G. (1987) Anal. Biochem. 166, 368-379
23. Matsudaira, P. (1987) J. Biol Chem. 262, 10035-10038
24. Bradford, M. M. (1976) Anal Biochem 72, 248-254
25. Hasnain, S., Hirama, T., Tam, A. & Mort, J. S. (1992) J. Biol. Chem. 267, 4713-4721
26. Marshall, R D. (1972) Annu. Rev. Biochem 41, 673-702
27. Bause, E (1983) Biochem J 209, 331-336
28. Felleisen, R. (1990) Molekular biologische Ansätze zur Immundiagnose der Bilharziose und Charakteristerung der hierfur verwendeten Antigene Sm31 und Sm32 von Schistosoma mansoni. Ph.D. thesis, University of Heidelberg, Germany
29. Ernst, J. F. (1986) DNA (NY) 5, 483-491
30. Hitzeman, R. A., Chen, C. Y., Dowbenko, D. J., Renz, M E., Liu, C., Pai, R., Simpson, N. J , Kohr, W. J , Singh, W. J , Chisholm, V , Hamilton, R & Chang, C. N. (1990) Methods Enzymol 185, 421-440
31. Hochuli, E., Bannwarth, W., Dobeli, H., Gentz, R. & Stuber, D. (1988) Bio/ Technology 6, 1321-1325
32. Jones, E. W. (1991) Methods Enzymol. 194, 428-453
33. Hirsch, H H., Rendueles, P. S & Wolf, D. H. (1989) in Molecular and Cell Biology of Yeast (Walton, E. F. & Yarranton, G. T., eds) pp. 134-200, Blackie, Glasgow
34. Rowan, A. D., Mason, P., Mach, L. & Mort, J. S (1992) J Biol. Chem. 267, 15993-15999
35. Mach, L., Mort, J. S. & Glössl, J. (1994) J. Biol. Chem 269, 13030-13055
36. Vernet, T , Khouri, H. E., Laflamme, P., Tessier, D. C., Musil, R., Gour-Salin, B J , Storer, A. C. & Thomas, D. Y. (1991) J. Biol. Chem. 266, 21451-21457
37. Fox, T., de Miguel, E., Mort, J S., and Storer, A. C. (1992) Biochemistry 31, 12571-12576
38. Duffaud, G D., March, P. E. & Inouye, M. (1987) Methods Enzymol. 153, 492-507
39. Moir, D. T. & Davidow, L. S. (1991) Methods Enzymol. 194, 491-507
40. Fuller, R. S., Sterne, R. E. & Thorner, J (1988) Annu. Rev Physiol. 50, 345-362
41. Koelsch, G., Mares, M., Metcalf, P. & Fusek, M (1994) FEBS Lett. 343, 6-10
42. Goldberg, D. E., Slater, A. F. G., Beavis, R., Chait, B., Cerami, A. & Henderson, G B. (1991) J. Exp. Med. 173, 961-969
43. Stoll, V. S. & Blanchard, J. S. (1990) Methods Enzymol 182, 24-38
44. Barrett, A. J. & Kirschke, H. (1981) Methods Enzymol. 80, 535-561
45. Kuhelj, R., Dolinar, M., Pungercar, J. & Turk, V. (1995) Eur. J. Biochem. 229, 533-539
46. Polgar, L. & Csoma, C. (1987) J. Biol. Chem. 262, 14448-14453
47. Dalton, J. P., Smith, A M., Clough, K. A. & Brindley, P. J. (1995) Parasitol. Today 11, 299-303
48. Bogitsh, B. J , Kirschner, K. F. & Rotmans, J. P. (1992) J. Parasitol. 78, 454-459
49. Gluzman, I. Y., Francis, S. E , Oksman, A., Smith, C. E., Duffin, K. L. & Goldberg, D. E. (1994) J. Clin. Invest. 93, 1602-1608
50. Grant, C. T. & Senft, A. W. (1971) Comp Biochem. Physiol. 38, 663-678
51. Sauer, M. C. V. & Senft, A. W. (1972) Comp. Biochem. Physiol. 42, 205-220
52. Barrett, A. J., Kembhavi, A. A., Brown, M. A , Kirschke, H., Knight, C. G , Tamai, M. & Hananda, K. (1982) Biochem. J. 201, 189-198
53. Dixon, H. B. F. & McIntosh, R. (1967) Nature 213, 399-400
54. Winterbourn, C. (1990) Methods Enzymol. 186, 265-272
55. El Meanawy, M. A., Aji, T., Phillips, N. F. B., Davis, E. E., Salata, R. A., Malhotra, I , McClain, D., Aikawa, M. & Davis, A. H. (1990) Am J. Trop. Med. Hyg. 43, 67-78