Comparison of sequencing the D2 region of the large subunit ribosomal RNA gene (MicroSEQ®) versus the internal transcribed spacer (ITS) regions using two public databases for identification of common and uncommon clinically relevant fungal species

Journal of Microbiological Methods

Volumn 140, Issue , 2017, Pages 40-46

  Arbefeville, S ^a   Harris, A ^a   Ferrieri, P ^a  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

Author keywords

D2 region of the large subunit rRNA gene; GenBank; Identification; Internal transcribed spacer (ITS) regions; Mould; MycoBank; Sequencing

Indexed keywords

INTERNAL TRANSCRIBED SPACER; LARGE SUBUNIT RIBOSOMAL RNA; FUNGAL DNA; RIBOSOMAL SPACER DNA; RIBOSOME DNA;

ANALYTICAL EQUIPMENT; ARTICLE; CONSENSUS SEQUENCE; CONTROLLED STUDY; FUNGAL GENE; FUNGUS IDENTIFICATION; FUNGUS ISOLATION; GENBANK; GENUS; LSU RRNA GENE; MYCOBANK; NONHUMAN; PRIORITY JOURNAL; SEQUENCE DATABASE; SPECIES IDENTIFICATION; COMPARATIVE STUDY; DIAGNOSTIC KIT; DNA SEQUENCE; EVALUATION STUDY; FUNGUS; GENETIC DATABASE; GENETICS; HIGH THROUGHPUT SEQUENCING; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGY; MYCOSIS; NUCLEIC ACID DATABASE; POLYMERASE CHAIN REACTION; PROCEDURES; RNA GENE;

DATABASES, GENETIC; DATABASES, NUCLEIC ACID; DNA, FUNGAL; DNA, RIBOSOMAL; DNA, RIBOSOMAL SPACER; FUNGI; GENES, RRNA; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; MYCOSES; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; SEQUENCE ANALYSIS, DNA;

EID: 85021860459     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2017.06.015     Document Type: Article

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