Identification of inhibitors of the association of ZAP-70 with the T cell receptor by high-throughput screen

SLAS Discovery

Volumn 22, Issue 3, 2017, Pages 324-331

  Visperas, Patrick R ^a,e   Wilson, Christopher G ^c   Winger, Jonathan A ^a,f   Yan, Qingrong ^a,g   Lin, Kevin ^a   Arkin, Michelle R ^c   Weiss, Arthur ^d   Kuriyan, John ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e PLEXXIKON INC   (United States)

^f Omniox   (United States)

^g JANSSEN RESEARCH AND DEVELOPMENT   (United States)

Author keywords

Covalent inhibitor; Fluorescence polarization (FP); Kinase; T cell; Time resolved fluorescence resonance energy transfer (TR FRET); ZAP 70

Indexed keywords

PROTEIN KINASE ZAP 70; T LYMPHOCYTE RECEPTOR; IMMUNOLOGIC FACTOR; LYMPHOCYTE ANTIGEN RECEPTOR; PEPTIDE; PROTEIN BINDING; PROTEIN KINASE INHIBITOR; RECOMBINANT PROTEIN; ZAP70 PROTEIN, HUMAN;

ARTICLE; CELL INTERACTION; CONTROLLED STUDY; FLUORESCENCE POLARIZATION; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; IC50; MOLECULAR CLONING; ORGAN TRANSPLANTATION; PROCESS OPTIMIZATION; PROTEIN BINDING; PROTEIN PURIFICATION; SENSITIVITY ANALYSIS; SIGNAL PROCESSING; SIGNAL TRANSDUCTION; ANTAGONISTS AND INHIBITORS; CELL FREE SYSTEM; CHEMISTRY; DRUG EFFECT; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE EXPRESSION; GENETICS; IMMUNOLOGY; MOLECULAR LIBRARY; PHARMACOLOGY; PHOSPHORYLATION; PROCEDURES;

CELL-FREE SYSTEM; FLUORESCENCE POLARIZATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE EXPRESSION; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; IMMUNOLOGIC FACTORS; PEPTIDES; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN KINASE INHIBITORS; RECEPTORS, ANTIGEN, T-CELL; RECOMBINANT PROTEINS; SIGNAL TRANSDUCTION; SMALL MOLECULE LIBRARIES; ZAP-70 PROTEIN-TYROSINE KINASE;

EID: 85021688214     PISSN: 24725552     EISSN: 24725560     Source Type: Journal    
DOI: 10.1177/1087057116681407     Document Type: Article

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