Intein Zymogens: Conditional Assembly and Splicing of Split Inteins via Targeted Proteolysis

Journal of the American Chemical Society

Volumn 139, Issue 24, 2017, Pages 8074-8077

  Gramespacher, Josef A ^a   Stevens, Adam J ^a   Nguyen, Duy P ^b   Chin, Jason W ^b   Muir, Tom W ^a  

^a PRINCETON UNIVERSITY   (United States)

^b MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMISTRY; SYNTHESIS (CHEMICAL);

BACTERIAL PATHOGENS; CHEMICAL BIOLOGY; DESIGN STRATEGIES; MULTIPLEXED SENSORS; PROTEIN SENSOR; SPLIT-INTEINS; TRANS-SPLICING; VIRAL INFECTIONS;

PROTEINS;

ENZYME PRECURSOR; INTEIN; SPLIT INTEIN; UNCLASSIFIED DRUG; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; PEPTIDE;

ARTICLE; BACTERIUM COLONY; CARBOXY TERMINAL SEQUENCE; CODON; HEK293T CELL LINE; NONHUMAN; POINT MUTATION; PROTEIN ASSEMBLY; PROTEIN DEGRADATION; PROTEIN SPLICING; STAPHYLOCOCCUS AUREUS; STATIC ELECTRICITY; TRANS SPLICING; VIRUS INFECTION; CHEMISTRY; HEK293 CELL LINE; HUMAN; METABOLISM;

ENZYME PRECURSORS; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; INTEINS; PEPTIDES; PROTEIN SPLICING; PROTEOLYSIS;

EID: 85021128747     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/jacs.7b02618     Document Type: Article

References (20)

1. Shah, N. H.; Muir, T. W. Chem. Sci. 2014, 5, 446 10.1039/C3SC52951G
2. Shah, N. H.; Muir, T. W. et al. J. Am. Chem. Soc. 2013, 135, 18673 10.1021/ja4104364
3. Iwai, H.; Züger, S.; Jin, J.; Tam, P. H. FEBS Lett. 2006, 580, 1853 10.1016/j.febslet.2006.02.045
4. Zettler, J.; Schütz, V.; Mootz, H. D. FEBS Lett. 2009, 583, 909 10.1016/j.febslet.2009.02.003
5. Shah, N. H.; Dann, G. P.; Vila-Perello, M.; Liu, Z.; Muir, T. W. J. Am. Chem. Soc. 2012, 134, 11338 10.1021/ja303226x
6. Carvajal-Vallejos, P.; Pallisse, R.; Mootz, H. D.; Schmidt, S. R. J. Biol. Chem. 2012, 287, 28686 10.1074/jbc.M112.372680
7. Mootz, H. D.; Muir, T. W. J. Am. Chem. Soc. 2002, 124, 9044 10.1021/ja026769o
8. Buskirk, A. R.; Ong, Y. C.; Gartner, Z. J.; Liu, D. R. Proc. Natl. Acad. Sci. U. S. A. 2004, 101, 10505 10.1073/pnas.0402762101
9. Yuen, C. M.; Rodda, S. J.; Vokes, S. A.; McMahon, A. P.; Liu, D. R. J. Am. Chem. Soc. 2006, 128, 8939 10.1021/ja062980e
10. Schwartz, E. C.; Saez, L.; Young, M. W.; Muir, T. W. Nat. Chem. Biol. 2007, 3, 50 10.1038/nchembio832
11. Peck, S. H.; Chen, I.; Liu, D. R. Chem. Biol. 2011, 18, 619 10.1016/j.chembiol.2011.02.014
12. Vila-Perelló, M.; Hori, Y.; Ribó, M.; Muir, T. W. Angew. Chem., Int. Ed. 2008, 47, 7764 10.1002/anie.200802502
13. Tyszkiewicz, A. B.; Muir, T. W. Nat. Methods 2008, 5, 303 10.1038/nmeth.1189
14. Böcker, J. K.; Friedel, K.; Matern, J. C.; Bachmann, A. L.; Mootz, H. D. Angew. Chem., Int. Ed. 2015, 54, 2116 10.1002/anie.201409848
15. Jung, D.; Sato, K.; Min, K.; Shigenaga, A.; Jung, J.; Otaka, A.; Kwon, Y. Chem. Commun. 2015, 51, 9670 10.1039/C5CC01067E
16. Ren, W.; Ji, A.; Ai, H. W. J. Am. Chem. Soc. 2015, 137, 2155 10.1021/ja508597d
17. Wong, S.; Mosabbir, A. A.; Truong, K. PLoS One 2015, 10, e0135965 10.1371/journal.pone.0135965
18. Paul, V. D. A novel bacteriophage Tail-Associated Muralytic Enzyme (TAME) from Phage K and its development into a potent antistaphylococcal protein BMC Microbiol. 2011, 11, 226
19. Nguyen, D. P.; Mahesh, M.; Elsässer, S. J.; Hancock, S. M.; Uttamapinant, C.; Chin, J. W. J. Am. Chem. Soc. 2014, 136, 2240 10.1021/ja412191m
20. Cheriyan, M.; Pedamallu, C. S.; Tori, K.; Perler, F. J. Biol. Chem. 2013, 288, 6202 10.1074/jbc.M112.433094