Characterization of a novel thermostable GH45 endoglucanase from Chaetomium thermophilum and its biodegradation of pectin

Journal of Bioscience and Bioengineering

Volumn 124, Issue 3, 2017, Pages 271-276

  Zhou, Qinzheng ^a   Ji, Peng ^a   Zhang, Jianye ^a   Li, Xue ^a   Han, Chao ^a  

^a SHANDONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

Bifunctional enzyme; Endoglucanase; Glycoside hydrolase family 45; Heterologous expression; Thermostability

Indexed keywords

AFFINITY CHROMATOGRAPHY; BIODEGRADATION; CELLULOSE; CLONING; ENZYMES; GENE ENCODING; HYDROLASES; HYDROLYSIS; MANGANESE; PURIFICATION; SUGARS;

BIFUNCTIONAL ENZYMES; ENDOGLUCANASES; GLYCOSIDE HYDROLASES; HETEROLOGOUS EXPRESSION; THERMOSTABILITY;

ENZYME ACTIVITY;

CALCIUM; CARBOXYMETHYLCELLULOSE; CELLULOSE; COPPER; GALACTURONIC ACID; GLUCAN SYNTHASE; GLYCOSIDASE; MAGNESIUM; MANGANESE; PECTIN; ZINC; CELLULASE; OLIGOSACCHARIDE; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIODEGRADATION; CHAETOMIUM; CHAETOMIUM THERMOPHILUM; CONTROLLED STUDY; ENZYME ACTIVITY; HETEROLOGOUS EXPRESSION; HYDROLYSIS; KOMAGATAELLA PASTORIS; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ALIGNMENT; THERMOSTABILITY; AMINO ACID SEQUENCE; CHEMISTRY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR MODEL; PICHIA;

CELLULOSE; CHROMATOGRAPHY; ENZYMES;

AMINO ACID SEQUENCE; CARBOXYMETHYLCELLULOSE SODIUM; CELLULASE; CELLULOSE; CHAETOMIUM; ENZYME STABILITY; HYDROLYSIS; MODELS, MOLECULAR; OLIGOSACCHARIDES; PECTINS; PICHIA; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

EID: 85019958536     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2017.03.017     Document Type: Article

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