Novel method to study pericyte contractility and responses to ischaemia in vitro using electrical impedance

Journal of Cerebral Blood Flow and Metabolism

Volumn 37, Issue 6, 2017, Pages 2013-2024

  Neuhaus, Ain A ^a   Couch, Yvonne ^a   Sutherland, Brad A ^a,c   Buchan, Alastair M ^a,b  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c UNIVERSITY OF TASMANIA   (Australia)

Author keywords

Contractility; Human brain vascular pericytes; iCelligence; Ischemia

Indexed keywords

ADENOSINE; ENDOTHELIN 1; LACTATE DEHYDROGENASE; NITROPRUSSIDE SODIUM;

ARTICLE; BRAIN BLOOD FLOW; BRAIN ISCHEMIA; BRAIN PERICYTE; CELL DEATH; CELL POPULATION; CELL PROLIFERATION; CEREBROVASCULAR ACCIDENT; CONTROLLED STUDY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IMPEDANCE; IN VITRO STUDY; IN VIVO STUDY; MRNA EXPRESSION ASSAY; MUSCLE CONTRACTILITY; PHENOTYPE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REPERFUSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TUNEL ASSAY; BRAIN; CELL CULTURE; CELL CULTURE TECHNIQUE; DRUG EFFECTS; HIGH THROUGHPUT SCREENING; MUSCLE CONTRACTION; PATHOPHYSIOLOGY; PERICYTE; PHYSIOLOGY; VASCULAR ENDOTHELIUM; VASCULAR SMOOTH MUSCLE; VASCULARIZATION; VASOCONSTRICTION; VASODILATATION;

BRAIN; BRAIN ISCHEMIA; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELLS, CULTURED; ELECTRIC IMPEDANCE; ENDOTHELIN-1; ENDOTHELIUM, VASCULAR; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MUSCLE CONTRACTION; MUSCLE, SMOOTH, VASCULAR; PERICYTES; VASOCONSTRICTION; VASODILATION;

EID: 85019770425     PISSN: 0271678X     EISSN: 15597016     Source Type: Journal    
DOI: 10.1177/0271678X16659495     Document Type: Article

References (37)

1. Hamilton NB, Attwell D, Hall CN. Pericyte-mediated regulation of capillary diameter: a component of neurovascular coupling in health and disease. Front Neuroenerg 2010; 2: 5.
2. Armulik A, Genove G, Mae M, et al. Pericytes regulate the blood-brain barrier. Nature 2010; 468: 557–561.
3. Bell RD, Winkler EA, Sagare AP, et al. Pericytes control key neurovascular functions and neuronal phenotype in the adult brain and during brain aging. Neuron 2010; 68: 409–427.
4. Winkler EA, Sengillo JD, Bell RD, et al. Blood-spinal cord barrier pericyte reductions contribute to increased capillary permeability. J Cereb Blood Flow Metab 2012; 32: 1841–1852.
5. Hellstrom M, Gerhardt H, Kalen M, et al. Lack of pericytes leads to endothelial hyperplasia and abnormal vascular morphogenesis. J Cell Biol 2001; 153: 543–553.
6. Virgintino D, Girolamo F, Errede M, et al. An intimate interplay between precocious, migrating pericytes and endothelial cells governs human fetal brain angiogenesis. Angiogenesis 2007; 10: 35–45.
7. Montagne A, Barnes SR, Sweeney MD, et al. Blood-brain barrier breakdown in the aging human hippocampus. Neuron 2015; 85: 296–302.
8. Sengillo JD, Winkler EA, Walker CT, et al. Deficiency in mural vascular cells coincides with blood-brain barrier disruption in Alzheimer’s disease. Brain Pathol 2013; 23: 303–310.
9. Winkler EA, Sengillo JD, Sullivan JS, et al. Blood-spinal cord barrier breakdown and pericyte reductions in amyotrophic lateral sclerosis. Acta Neuropathol 2013; 125: 111–120.
10. Hill RA, Tong L, Yuan P, et al. Regional blood flow in the normal and ischemic brain is controlled by arteriolar smooth muscle cell contractility and not by capillary pericytes. Neuron 2015; 87: 95–110.
11. Fernandez-Klett F, Offenhauser N, Dirnagl U, et al. Pericytes in capillaries are contractile in vivo, but arterioles mediate functional hyperemia in the mouse brain. Proc Natl Acad Sci U S A 2010; 107: 22290–22295.
12. Peppiatt CM, Howarth C, Mobbs P, et al. Bidirectional control of CNS capillary diameter by pericytes. Nature 2006; 443: 700–704.
13. Hall CN, Reynell C, Gesslein B, et al. Capillary pericytes regulate cerebral blood flow in health and disease. Nature 2014; 508: 55–60.
14. Roger VL, Go AS, Lloyd-Jones DM, et al. Heart disease and stroke statistics – 2012 update: a report from the American Heart Association. Circulation 2012; 125: e2–e220.
15. Mishra A, O’Farrell FM, Reynell C, et al. Imaging pericytes and capillary diameter in brain slices and isolated retinae. Nature Protoc 2014; 9: 323–336.
16. Dore-Duffy P. Isolation and characterization of cerebral microvascular pericytes. Method Mol Med 2003; 89: 375–382.
17. Bryan BA, D’Amore PA. Pericyte isolation and use in endothelial/pericyte coculture models. Method Enzymol 2008; 443: 315–331.
18. Kelley C, D’Amore P, Hechtman HB, et al. Microvascular pericyte contractility in vitro: comparison with other cells of the vascular wall. J Cell Biol 1987; 104: 483–490.
19. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001; 29: e45.
20. Dore-Duffy P. Pericytes: pluripotent cells of the blood brain barrier. Curr Pharmaceut Des 2008; 14: 1581–1593.
21. Winkler EA, Bell RD, Zlokovic BV. Pericyte-specific expression of PDGF beta receptor in mouse models with normal and deficient PDGF beta receptor signaling. Mol Neurodegen 2010; 5: 32.
22. Xi B, Wang T, Li N, et al. Functional cardiotoxicity profiling and screening using the xCELLigence RTCA cardio system. J Lab Automat 2011; 16: 415–421.
23. Haynes WG, Webb DJ. Endothelin: a long-acting local constrictor hormone. Br J Hosp Med 1992; 47: 340–349.
24. Bryan PT, Marshall JM. Adenosine receptor subtypes and vasodilatation in rat skeletal muscle during systemic hypoxia: a role for A1 receptors. J Physiol 1999; 514(Pt 1): 151–162.
25. Xi B, Yu N, Wang X, et al. The application of cell-based label-free technology in drug discovery. Biotechnol J 2008; 3: 484–495.
26. Chakravarthy U, McGinty A, McKillop J, et al. Altered endothelin-1 induced contraction and second messenger generation in bovine retinal microvascular pericytes cultured in high glucose medium. Diabetologia 1994; 37: 36–42.
27. Dehouck MP, Vigne P, Torpier G, et al. Endothelin-1 as a mediator of endothelial cell-pericyte interactions in bovine brain capillaries. J Cereb Blood Flow Metab 1997; 17: 464–469.
28. Morel NM, Dodge AB, Patton WF, et al. Pulmonary microvascular endothelial cell contractility on silicone rubber substrate. J Cell Physiol 1989; 141: 653–659.
29. Yamagishi S, Hsu CC, Kobayashi K, et al. Endothelin 1 mediates endothelial cell-dependent proliferation of vascular pericytes. Biochem Biophys Res Commun 1993; 191: 840–846.
30. McDonald DM, Bailie JR, Archer DB, et al. Characterization of endothelin A (ETA) and endothelin B (ETB) receptors in cultured bovine retinal pericytes. Invest Ophthalmol Vis Sci 1995; 36: 1088–1094.
31. Dore-Duffy P, Wang S, Mehedi A, et al. Pericyte-mediated vasoconstriction underlies TBI-induced hypoperfusion. Neurol Res 2011; 33: 176–186.
32. O’Farrell FM, Attwell D. A role for pericytes in coronary no-reflow. Nat Rev Cardiol 2014; 11: 427–432.
33. Matsugi T, Chen Q, Anderson DR. Adenosine-induced relaxation of cultured bovine retinal pericytes. Invest Ophthalmol Vis Sci 1997; 38: 2695–2701.
34. Puro DG. Physiology and pathobiology of the pericyte-containing retinal microvasculature: new developments. Microcirculation 2007; 14: 1–10.
35. Palmer RM, Ferrige AG, Moncada S. Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature 1987; 327: 524–526.
36. Martinez-Ruiz A, Cadenas S, Lamas S. Nitric oxide signaling: classical, less classical, and nonclassical mechanisms. Free Radic Biol Med 2011; 51: 17–29.
37. Yemisci M, Gursoy-Ozdemir Y, Vural A, et al. Pericyte contraction induced by oxidative-nitrative stress impairs capillary reflow despite successful opening of an occluded cerebral artery. Nat Med 2009; 15: 1031–1037.