Isolated microspore culture and its applications in plant breeding and genetics

Plant Tissue Culture: Propagation, Conservation and Crop Improvement

Volumn , Issue , 2016, Pages 487-507

  Shariatpanahi, Mehran E ^a   Ahmadi, Behzad ^a  

^a AGRICULTURAL BIOTECHNOLOGY RESEARCH INSTITUTE OF IRAN ABRII   (Iran)

Author keywords

Breeding; Doubled haploid; Genetics; Microspore embryogenesis; Stress

Indexed keywords

EID: 85018584257     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/978-981-10-1917-3_21     Document Type: Chapter

References (120)

1. Abdollahi MR, Corral-Martínez P, Mousavi A, Salmanian AH, Moini A, Seguí-Simarro JM (2009) An efficient method for transformation of pre- androgenic, isolated Brassica napus microspores involving microprojectile bombardment and Agrobacterium-mediated transformation. Acta Physiol Plant 31(6):1313-1317
2. Ahloowalia BS, Maluszynski M (2001) Induced mutations- a new paradigm in plant breeding. Euphytica 118:167-173
3. Ahmad I, Day JP, MacDonald MV, Ingram DS (1991) Haploid culture and UV mutagenesis in rapid-cycling Brassica napus for the generation of resistance to chlorsulfuron and Alternaria brassicicola. Ann Bot 67(6):521-525
4. Ahmadi B, Alizadeh K, Teixeira da Silva JA (2012a) Enhanced regeneration of haploid plantlets from microspores of Brassica napus L. using bleomycin, PCIB, and phytohormones. Plant Cell Tissue Organ Cult 109:525-533
5. Ahmadi B, Ghadimzadeh M, Moghaddam AF, Alizadeh K, Teixeira da Silva JA (2012b) Bud length, plating density, and incubation time on microspore embryogenesis in Brassica napus. Int J Veg Sci 18:346-357
6. Ahmadi B, Shariatpanahi ME, Teixeira da Silva JA (2014) Efficient induction of microspore embryogenesis using abscisic acid, jasmonic acid and salicylic acid in Brassica napus L. Plant Cell Tissue Organ Cult 116:343-351
7. Ajisaka H, Kuginuki Y, Yui S, Enomoto S, Hirai M (2001) Identification and mapping of a quantitative trait locus controlling extreme late bolting in Chinese cabbage (Brassica rapa L. ssp. Pekinensis syn. campestris L.) using bulked segregant analysis. Euphytica 118:75-81
8. Asif M (2013) History, production methods, and types of haploids. Prog Oppor Doubled Haploid Plant 6:1-6
9. Asif M, Eudes F, Goyal A, Amundsen E, Randhawa H, Spaner D (2013a) Organelle antioxidants improve microspore embryogenesis in wheat and triticale. In Vitro Cell Dev Biol Plant 49(5):489-497
10. Asif M, Eudes F, Randhawa H, Amundsen E, Yanke J, Spaner D (2013b) Cefotaxime prevents microbial contamination and improves microspore embryogenesis in wheat and triticale. Plant Cell Rep 32(10):1637-1646
11. Ayed OS, Buyser JD, Picard E, Trifa Y, Amara HS (2010) Effect of pre-treatment on isolated microspore culture ability in durum wheat (Triticum turgidum subsp. durum Desf.). J Plant Breed Crop Sci 2(2):30-38
12. Bal U, Elliatioglu S, Abak K (2009) Induction of symmetrical nucleus division and multi-nucleate structures in microspores of eggplant (Solanum melongena L.) cultured in vitro. Sci Agric 66(4):535-539
13. Barro F, Fernandez-Escobar J, De la Vega M, Martin A (2001) Doubled haploid lines of Brassica carinata with modified erucic acid content through mutagenesis by EMS treatment of isolated microspores. Plant Breed 120:262-264
14. Barro F, Fernandez-Escobar J, De la Vega M, Martin A (2002) Modification of glucosinolate and erucic acid contents in doubled haploid lines of Brassica carinata by UV treatment of isolated microspores. Euphytica 129:1-6
15. Birchler JA, Auger DL, Riddle NC (2003) In search of the molecular basis of heterosis. Plant Cell 15:2236-2239
16. Bohanec B (2009) Doubled haploids via gynogenesis. In: Touraev A, Forster BP, Jain SM (eds) Advances in haploid production in higher plants. Springer Science+Business Media B.V, Dordrecht, pp 35-46. ISBN 978-1-4020-8853-7
17. Bourgin JP, Nitsch JP (1967) Obtention de Nicotiana haploids a partir d’etamines cultivees in vitro. Ann Physiol Veg 9:377-382
18. Brew-Appiah RA, Ankrah N, Liu W, Konzak CF, von Wettstein D, Rustgi S (2013) Generation of doubled haploid transgenic wheat lines by microspore transformation. PLoS One. doi: 10.1371/journal.pone.0080155
19. Cegielska-Taras T, Pniewski T, Szala L (2008) Transformation of microspore-derived embryos of winter oilseed rape (Brassica napus L.) by using Agrobacterium tumefaciens. J Appl Genet 49(4):343-347
20. Chan J, Pauls KP (2007) Brassica napus Rop GTPases and their expression in microspore cultures. Planta 225:469-484
21. Chen G, Geng J, Rahman M, Liu X, Tu J, Fu T, Li G, McVetty PBE, Tahir M (2010) Identification of QTL for oil content, seed yield, and flowering time in oilseed rape (Brassica napus). Euphytica 175:161-174
22. Clapham D, Manders G, Yibrah HS, von Arnold S (1995) Enhancement of short- and medium-term expression of transgenes in embryogenic suspensions of Picea abies (L.) Karst. J Exp Bot 46:655-662
23. Cloud V, Chan YL, Grubb J, Budke B, Bishop DK (2012) Rad51 is an accessory factor for Dmc1-mediated joint molecule formation during meiosis. Science 337(6099):1222-1225
24. Cloutier S, Ragupathy R, Niu Z, Duguid S (2011) SSR-based linkage map of flax (Linum usitatissimum L.) and mapping of QTLs underlying fatty acid composition traits. Mol Breed 28:437-451
25. Collard BCY, Mackill DJ (2008) Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Philos Trans R Soc B 363:557-572
26. Collard BCY, Jahufer MZZ, Brouwer JB, Pang ECK (2005) An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: the basic concepts. Euphytica 142:169-196
27. Dirks R, van Dun K, de Soon CB, van der Berg M, Lelivelt CLC, Voermans W et al (2009) Reverse breeding: a novel breeding approach based on engineered meiosis. Plant Biotechnol J 7:837845
28. Dubas E, Wedzony M, Petrovska B, Salaj J, Żur I (2010) Cell structural reorganization during induction of androgenesis in isolated microspore cultures of triticale (x Triticosecale Wittm.). Acta Biol Cracov Bot 52(1):73-86
29. Dubas E, Custers J, Kieft H, Wedzony M, van Lammeren AA (2011) Microtubule configurations and nuclear DNA synthesis during initiation of suspensor-bearing embryos from Brassica napus cv. Topas microspores. Plant Cell Rep 30(11):2105-2116
30. Dubas E, Janowiak F, Krzewska M, Hura T, Żur I (2013) Endogenous ABA concentration and cytoplasmic membrane fluidity in microspores of oilseed rape (Brassica napus L.) genotypes differing in responsiveness to androgenesis induction. Plant Cell Rep 32(9):1465-1475
31. Dubas E, Moravčíková J, Libantová J, Matušíková I, Benková E, Żur I, Krzewska M (2014) The influence of heat stress on auxin distribution in transgenic B. napus microspores and microspore-derived embryos. Protoplasma 251:1077-1087
32. Dunwell JM (2010) Haploids in flowering plants: origins and exploitation. Plant BiotechnolJ 8:377-424
33. Feng-Ian Z, Takahata Y (1999) Microspore mutagenesis and in vitro selection for resistance to soft rot disease in soft rot in Chinese cabbage (Brassica campestris L. spp. pekinensis). Breed Sci 49:161-166
34. Fennell A, Hauptmann R (1992) Electroporation and PEG delivery of DNA into maize micro-spores. Plant Cell Rep 11:567-570
35. Ferrie AMR, Caswell KL (2011) Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell Tissue Organ Cult 104:301-309
36. Ferrie AMR, Möllers C (2010) Haploids and doubled haploids in Brassica spp. for genetic and genomic research. Plant Cell Tiss Org Cult 104:375-386
37. Ferrie AMR, Taylor DC, MacKenzie SL, Rakow G, Raney JP, Keller WA (2008) Microspore muta-genesis of Brassica species for fatty acid modifications: a preliminary evaluation. Plant Breed 127:501-506
38. Folling L, Olesen A (2001) Transformation of wheat (Triticum aestivum L.) microspore derived callus and microspores by particle bombardment. Plant Cell Rep 20(7):629-636
39. Forster BP, Thomas WTB (2003) Doubled haploids in genetic mapping and genomic. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. Kluwer Academic Publishers, Dordrecht, pp 367-390
40. Forster BP, Heberle-Bors E, Kasha KJ, Touraev A (2007) The resurgence of haploids in higher plants. Trends Plant Sci 12:368-375
41. Ge Y, Wang T, Wang N, Wang Z, Liang C, Ramchiary N, Choi SR, Lim YP, Piao ZY (2012) Genetic mapping and localization of quantitative trait loci for chlorophyll content in Chinese cabbage (Brassica rapa ssp. pekinensis). Sci Horticult 147:42-48
42. Germana MA (2006) Doubled haploid production in fruit crops. Plant Cell Tiss Org Cult 86:131-146
43. Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 42:909-930
44. Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature 204:497
45. Gurushidze M, Hensel G, Hiekel S, Schedel S, Valkov V et al (2014) True-breeding targeted gene knock-out in barley using designer TALE-nuclease in haploid cells. PLoS One 9(3):e92046. doi: 10.1371/journal.pone.0092046
46. Han SY, Zhang HY, Yang ML, Zhao TJ, Gai JY, Yu DY (2007) Screening of mutants and construction of mutant population in soybean “Nannong 86-4". Acta Agron Sin 33:2059-2062
47. Hansel G, Oleszczuk S, Daghma DES, Zimny J, Melzer M, Kumlehn J (2012) Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x Triticosecale Wittmack). BMC Plant Biol 12:171
48. Hoseini M, Ghadimzadeh M, Ahmadi B, Teixeira da Silva JA (2014) Effects of ascorbic acid, alpha-tocopherol, and glutathione on microspore embryogenesis in Brassica napus L. In Vitro Cell Dev Biol Plant 50:26-35
49. 60 Co γ-ray mutagenesis of isolated microspore cultures. Plant Breed 133:480-488
50. Hussain B, Khan MA, Ali Q, Shaukat S (2012) Double haploid production is the best method for genetic improvement and genetic studies of wheat. Int J Agro Vet Med Sci 6(4):216-228
51. Ilyas M, Ilyas N, Arshad M, Kazi AG, Kazi AM, Waheed A (2014) QTL mapping of wheat doubled for chlorophyll content and chlorophyll fluorescence kinetics under drought stress imposed at anthesis stage. Pak J Bot 46(5):1889-1897
52. Immonen S, Anttila H (1996) Success in rye anther culture. Vortr Pflanzenzüchtg 35:237-2441
53. Indrianto A, Mariani TS, Sari DA (2014) Induction of embryogenic microspore in oil palm (Elaeis guineensis Jacq) by starvation and temperature stress. Asia J Appl Sci 2(5):668-677
54. Ingram HM, Power JB, Lowe KC, Davey MR (1999) Optimisation of procedures for microprojec-tile bombardment of microspore-derived embryos in wheat. Plant Cell Tiss Org Cult 57:207-2101
55. slam SMS (2010) The effect of colchicine pretreatment on isolated microspore culture of wheat (Triticum aestivum L.). Aust J Crop Sci 4(9):660-665
56. Janska A, Zelenkova S, Klima M, Vyvadilova M, Prasil IT (2010) Freezing tolerance and proline content of in vitro selected hydroxyproline resistant winter oilseed rape. Czech J Genet Plant Breed 46(1):35-40
57. Jardinaut MF, Souvre A, Alibert G (1993) Transient GUS gene expression in Brassica napus electroporated microspores. Plant Sci 93:177-184
58. Jardinaut MF, Souvre A, Beckert M, Alibert G (1995) Optimization of DNA transfer and transient b-glucuronidase expression in electroporated maize (Zea mays L.) microspores. Plant Cell Rep 15:55-58
59. Jauhar PP (2003) Haploid and doubled haploid production in durum wheat by anther culture. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants: a manual. Kluwer Academic Publishers, Dordrecht, pp 167-172
60. Jin M, Lee SS, Ke L, Kim JS, Seo MS, Sohn SH, Park BS, Bonnema G (2014) Identification and mapping of a novel dominant resistance gene, TuRB07 to Turnip mosaic virus in Brassica rapa. Theor Appl Genet 127:509-519
61. Jung KH, An G (2013) Functional characterization of rice genes using a gene-indexed T-DNA insertional mutant population. Rice Protoc 956:57-67
62. Kim M, Jang IC, Kim JA, Park EJ, Yoon M, Lee Y (2008) Embryogenesis and plant regeneration of hot pepper (Capsicum annuum L.) through isolated microspore culture. Plant Cell Rep 27(3):425-434
63. Klima M, Vyvadilova M, Kucera V (2008) Chromosome doubling effects of selected antimitotic agents in Brassica napus microspore culture. Czech J Genet Plant Breed 44(1):30-36
64. Lantos C, Juhász AG, Mihály PVR, Kristóf Z, Paul J (2012) Androgenesis induction in microspore culture of sweet pepper (Capsicum annuum L.). Plant Biotechnol Rep 6(2):123-132
65. Li JR, Zhuang FY, Qu CG, Hu H, Zhao ZW, Mao JH (2012) Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.). Plant Cell Tiss Org Cult 112(3):275-287
66. Lionneton E, Ravera S, Sanchez L, Aubert G, Delourme R, Ochatt S (2002) Development of an AFLP-based linkage map and localization of QTLs for seed fatty acid content in condiment mustard (Brassica juncea). Genome 45:1203-1215
67. Liu S, Wang H, Zhang J, Fitt BDL, Xu Z, Evans N, Liu Y, Yang W, Guo X (2005) In vitro mutation and selection of doubled-haploid Brassica napuslines with improved resistance to Sclerotinia sclerotiorum. Plant Cell Rep 24:133-144
68. Lou P, Zhao J, He H, Hanhart C, Carpio DND, Verkerk R, Custers J, Koorneef M, Bonnema G (2008) Quantitative trait loci for glucosinolate accumulation in Brassica rapa leaves. New Phytol 179:1017-1032
69. Ma H (2005) Molecular genetic analyses of microsporogenesis and microgametogenesis in flowering plants. Annu Rev Plant Biol 56:393-434
70. Martinez V, Thorgaard G, Robison B, Sillanpää MJ (2005) An application of Bayesian QTL mapping to early development in double haploid lines of rainbow trout including environmental effects. Genet Res 86(3):209-221
71. McClinchey SL, Kott LS (2008) Production of mutants with high cold tolerance in spring canola (Brassica napus). Euphytica 162:51-67
72. Mentewab A, Letellier V, Marque C, SarrafiA (1999) Use of anthocyanin biosynthesis stimulatory genes as markers for the genetic transformation of haploid embryos and isolated microspores in wheat. Cereal Res Commun 27(1-2):17-24
73. Mishra VK, Goswami R (2014) Haploid production in higher plant. Int J Chem Biol Sci 1(1):25-45
74. Muñoz-Amatriaín M, Sevensson JT, Castillo AM, Cistue L, Close TJ (2006) Transcriptome analysis of barely anthers: effect of mannitol treatment on microspore embryogenesis. Physiol Plant 127:551-560
75. Nehlin L, Mollers C, Bergman P, Glimelius K (2000) Transient f3 -gus and gfp gene expression and viability analysis of microprojectile bombarded microspores of Brassica napus L. J Plant Physiol 156:175-183
76. Nishioka M, Tamura K, Hayashi M, Fujimori Y, Ohkawa Y, Kuginuki Y, Harada K (2005) Mapping of QTLs for bolting time in Brassica rapa(syn. campestris) under different environmental conditions. Breed Sci 55:127-133
77. Obert B, Ponya Z, Pretova A, Barnabas B (2004) Optimization of electroporation conditions for maize microspores. Maydica 49:15-19
78. Palmgren MG, Edenbrandt AK, Elizabeth S, Andersen VMM, Landes X, Østerberg JT, Falhof J et al (2014) Are we ready for back-to-nature crop breeding? Trend Plant Sci 1238:1-10
79. Parez-Prat E, van Lookeren Compagne MM (2002) Hybrid seed production and the challenge of propagating malesterile plants. Trends Plant Sci 7(5):199-203
80. Pink D, Bailey L, McClement S, Hand P, Mathas E, Buchanan-Wollaston V, Astley D, King G, Teakle G (2008) Double haploids, markers and QTL analysis in vegetable Brassicas. Euphytica 164:509-514
81. Polsoni L, Kott LS, Beversdorf WD (1987) Large-scale microspore culture technique for mutation -selection studies in Brassica napus. Can J Bot 66:1681-1685
82. Portemer V, Renne C, Guillebaux A, Mercier R (2015) Large genetic screens for gynogenesis and androgenesis haploid inducers in Arabidopsis thalianafailed to identify mutants. Front Plant Sci 6:147. doi: 10.3389/fpls.2015.00147
83. Prem D, Solís MT, Bárány I, Rodríguez-Sanz H, Risueño MC, Testillano PS (2012a) A new micro-spore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus. BMC Plant Biol 12:127. doi: 10.1186/1471-2229-12-127
84. Prem D, Gupta K, Agnihotri A (2012b) Harnessing mutant donor plants for microspore culture in Indian mustard [Brassica juncea (L.) Czern and Coss]. Euphytica 184:207-222
85. Qin YH, Teixeira da Silva JA, Bi JH, Zhang SL, Hu GB (2011) Response of in vitro strawberry to antibiotics. Plant Growth Regul 65:183-193
86. Resch T, Touraev A (2011) Pollen transformation technologies. In: Stewart N, Touraev A, CitovskyV, Tzfira T, (eds) Plant transformation technologies. Wiley-Blackwell, Chichester, pp 83-91
87. Ribarits A, Mamun ANK, Li S, Resch T, Fiers M, Heberle-Bors E, Liu CM, Touraev A (2007) Combination of reversible male sterility and doubled haploid production by targeted inactivation of cytoplasmic glutamine synthetase in developing anthers and pollen. Plant Biotechnol J 5:483-494
88. Rodríguez-Sanz H, Manzanera J, Solis M, Gómez-Garay A, Pintos B, Risueño MC, Testillano PS (2014) Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suber L. BMC Biol. doi: 10.1186/s12870-014-0224-4
89. Rodríguez-Serrano M, Bárány I, Prem D, Coronado MJ, Risueño MC, Testillano PS (2011) NO, ROS, and cell death associated with caspase-like activity increase in stress-induced microspore embryogenesis of barley. J Exp Bot 63:2007-2024
90. Rygulla W, Snowdon RJ, Friedt W, Happstadius I, Cheung WY, Chen D (2008) Identification of quantitative trait loci for resistance against Verticillium longisporum in oilseed rape (Brassica napus). Genet Res 98(2):215-221
91. Scofield S, Dewitte W, Nieuwland J, Murray JA (2013) The Arabidopsis homeobox gene SHOOTMERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity. Plant J 75(1):53-66
92. Seyis F, Aydin E, Catal MI (2014) Haploids in the improvement of crucifers. Turk J Agric Nat Sci 2:1419-1424
93. Shariatpanahi ME, Bal U, Heberle-Bors E, Touraev A (2006) Stresses applied for the re-programming of plant microspores towards in vitro embryogenesis. Physiol Plant 127:519-534
94. Sharma P, Jha AB, Dubey RS, Pessarakli M (2012) Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J Bot. doi: 10.1155/2012/217037
95. Shim YS, Pauls KP, Kasha KJ (2009) Transformation of isolated barely (Hordeum vulgare L.) microspores: II: timing of pretreatment and temperatures relative to results of bombardment. Gemone 52:175-190
96. Soriano M, Cistue L, Valles MP, Castillo AM (2007) Effects of colchicine on anther and micro-spore culture of bread wheat (Triticum aestivum L.). Plant Cell Tiss Org Cult 91(3):225-234
97. Soriano M, Li H, Boutilier K (2013) Microspore embryogenesis: establishment of embryo identity and pattern in culture. Plant Reprod 26:181-196
98. Stasolla C, Belmonte MF, Tahir M, Elhiti M, Khamiss K, Joosen R, Maliepaard C, Sharpe A, Gjetvaj B, Boutilier K (2008) Buthionine sulfoximine (BSO)-mediated improvement in cultured embryo quality in vitro entails changes in ascorbate metabolism, meristem development and embryo maturation. Planta 228(2):255-272
99. Suwabe K, Tsukazaki H, Iketani H, Hatakeyama K, Fujimura M, Nunome T, Fukuoka H, Matsumoto S, Hirai M (2003) Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L. Theor Appl Genet 107:997-1002
100. Swanson EB, Coumans MP, Brown GL, Patel JD, Beversdorf WD (1988) The characterization of herbicide tolerant plants in Brassica napus L. after in vitro selection of microspores and protoplasts. Plant Cell Rep 7:83-87
101. Swanson EB, Herrgesell MJ, Arnoldo M, Sippell DW, Wong RSC (1989) Microspore mutagenesis and selection: canola plants with field tolerance to the imidazolinones. Theor Appl Genet 78:525-530
102. Szarejko I, Forster BP (2007) Doubled haploidy and induced mutation. Euphytica 130:359-370
103. Takahata Y, Fukuoka H, Wakui K (2005) Utilization of microspore-derived embryos. In: Plamer CE, Keller WA, Kasha KJ (eds) Haploids in crop improvement II. Springer, Berlin, pp 153-169
104. Toppino L, Kooiker M, Lindner M, Dreni L, Rotino GL, Kater MM (2011) Reversible male sterility in eggplant (Solanum melongena L.) by artificial microRNA-mediated silencing of general transcription factor genes. Plant Biotechnol J 9:684-692
105. Travella S, Ross SM, Harden J, Everett C, Snape JW, Harwood WA (2005) A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques. Plant Cell Rep 23:780-789
106. Wang NL, Long T, Yao W, Xiong LZ, Zhang QF, Wu CY (2013) Mutant resources for the functional analysis of the rice genome. Mol Plant 6:596-604
107. Wijnker E, van Dun K, de Snoo CB, Lelivelt CLC, Keurentjes JJB, Naharudin NS, Ravi M, Chan SWL, de Jong H, Dirks R (2012) Reverse breeding in Arabidopsis thaliana generates homozygous parental lines from a heterozygous plant. Nat Genet 44:467-470
108. Wu J, Cai G, Tu J, Li L, Liu S et al (2013) Identification of QTLs for resistance to Sclerotinia stem rot and BnaC.IGMT5.a as a candidate gene of the major resistant QTL SRC6 in Brassica napus. PLoS One 8(7):e67740. doi: 10.1371/journal.pone.0067740
109. Xu YH, Chen F, Dong ZD, Cui DQ (2010) Construction and analysis of EMS induced mutant library of hexaploid wheat cultivar Yunong 201. J Tritic Crops 30:625-629
110. Yang X, Yu YJ, Zhang FL, Zou ZR, Zhao XY, Zhang DS, Xu JB (2007) Linkage map construction and quantitative trait loci analysis for bolting based on a double haploid population of Brassica rapa. J Integr Plant Biol 49(5):664-671
111. Yang P, Shu S, Chen L, Xu J, Wu J, Liu K (2012) Identification of a major QTL for silique length and seed weight in oilseed rape (Brassica napus L.). Theor Appl Genet 125:285-296
112. Yao QA, Simion E, William M, Krochko J, Kasha KJ (1997) Biolistic transformation of haploid isolated microspores of barley (Hordeum vulgare L.). Genome 40:570-581
113. Yu F, Lydiate DJ, Rimmer SR (2005) Identification of two novel genes for blackleg resistance inBrassica napus. Theor Appl Genet 110:969-979
114. Yu F, Lydiate DJ, Rimmer SR (2008) Identification and mapping of a third blackleg resistance locus in Brassica napus derived from B. rapa subsp. sylvestris. Genome 51:64-72
115. Yu S, Zhang F, Yu R, Zou Y, Qi J, Zhao X, Yu Y, Zhang D, Li L (2009) Genetic mapping and localization of a major QTL for seedling resistance to downy mildew in Chinese cabbage (Brassica rapa ssp. pekinensis). Mol Breed 23:573-590
116. Zhang FL, Wang M, Liu XC, Zhao XY, Yang JP (2008) Quantitative trait loci analysis for resistance against Turnip mosaic virus based on a doubled-haploid population in Chinese cabbage. Plant Breed 127:82-86
117. Ziemienowicz A, Shim Y-S, Matsuoka A, Eudes F, Kovalchuk I (2012) A novel method of trans-gene delivery into Triticale plants using the Agrobacterium transferred DNA-derived nano-complex. Plant Physiol 158:1503-1513
118. Żur I, Dubas E, Golemier F, Szechynska-Hebda M, Golehiowska G, Wedzony M (2009) Stress-related variation in antioxidative enzymes activity and cell metabolism efficiency associated with embryogenesis induction in isolated microspore culture of triticale (× Triticosecale Wittm.). Plant Cell Rep 28:1279-1287
119. Żur I, Krzewska M, Dubas E, Gołębiowska-Pikania G, Janowiak F, Stojalowski S (2012) Molecular mapping of loci associated with abscisic acid accumulation in triticale (× Triticosecale Wittm.) anthers in response to low temperature stress inducing androgenic development. Plant Growth Regul 68(3):483-492
120. Żur L, Dubas E, Krzewska M, Janowiak F, Hura K, Pociecha E, Baczek-Kwinta R, Plazek A (2014) Antioxidant activity and ROS tolerance in triticale (× Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis. Plant Cell Tiss Org Cult 119:79-94