Comparison of 11 phenotypic assays for accurate detection of carbapenemase- producing enterobacteriaceae

Journal of Clinical Microbiology

Volumn 55, Issue 4, 2017, Pages 1046-1055

  Tamma, Pranita D ^a   Opene, Belita N A ^a   Gluck, Andrew ^a   Chambers, Krizia K ^a   Carroll, Karen C ^a   Simner, Patricia J ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Carbapenem resistant organisms; Carbapenemase producing organisms; Prevalence

Indexed keywords

CARBAPENEM; CARBAPENEMASE; BACTERIAL PROTEIN; BETA LACTAMASE;

ANTIBIOTIC SENSITIVITY; ARTICLE; ASSAY; BACTERIUM DETECTION; BACTERIUM ISOLATE; BORONIC ACID SYNERGY TEST; CARBAPENEMASE PRODUCING ENTEROBACTERIACEAE; COHORT ANALYSIS; CONTROLLED STUDY; DIAGNOSTIC KIT; DIAGNOSTIC TEST; DIAGNOSTIC TEST ACCURACY STUDY; FALSE POSITIVE RESULT; GENOTYPE; GOLD STANDARD; INTERMETHOD COMPARISON; MODIFIED CARBAPENEM INACTIVATION METHOD; MODIFIED HODGE TEST; MOLECULAR DIAGNOSIS; MOLECULAR EPIDEMIOLOGY; NONHUMAN; PHENOTYPE; PROSPECTIVE STUDY; RETROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; TURNAROUND TIME; COMPARATIVE STUDY; ENTEROBACTERIACEAE; ENZYMOLOGY; EVALUATION STUDY; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION; PROCEDURES;

BACTERIAL PROTEINS; BACTERIOLOGICAL TECHNIQUES; BETA-LACTAMASES; ENTEROBACTERIACEAE; HUMANS; PROSPECTIVE STUDIES; RETROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY;

EID: 85016198921     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02338-16     Document Type: Article

References (21)

1. Tamma PD, Goodman KE, Harris AD, Tekle T, Robert A, Taiwo A, Simner PJ. 2017. Comparing the outcomes of patients with carbapenemaseproducing and non-carbapenemase-producing carbapenem-resistant enterobacteriaceae bacteremia. Clin Infect Dis 64:257-264. https://doi.org/10.1093/cid/ciw741.
2. Nordmann P, Naas T, Poirel L. 2011. Global spread of carbapenemaseproducing Enterobacteriaceae. Emerg Infect Dis 17:1791-1798. https://doi.org/10.3201/eid1710.110655.
3. Tamma PD, Huang Y, Opene BN, Simner PJ. 2016. Determining the optimal carbapenem MIC that distinguishes carbapenemase-producing and non-carbapenemase-producing carbapenem-resistant Enterobacteriaceae. Antimicrob Agents Chemother 60:6425-6429. https://doi.org/10.1128/AAC.00838-16.
4. Nordmann P, Poirel L, Dortet L. 2012. Rapid detection of carbapenemaseproducing Enterobacteriaceae. Emerg Infect Dis 18:1503-1507. https://doi.org/10.3201/eid1809.120355.
5. Carvalhaes CG, Picao RC, Nicoletti AG, Xavier DE, Gales AC. 2010. Cloverleaf test (modified Hodge test) for detecting carbapenemase production in Klebsiella pneumoniae: be aware of false positive results. J Antimicrob Chemother 65:249-251. https://doi.org/10.1093/jac/dkp431.
6. van der Zwaluw K, de Haan A, Pluister GN, Bootsma HJ, de Neeling AJ, Schouls LM. 2015. The carbapenem inactivation method (CIM), a simple and low-cost alternative for the Carba NP test to assess phenotypic carbapenemase activity in gram-negative rods. PLoS One 10:e0123690. https://doi.org/10.1371/journal.pone.0123690.
7. Papagiannitsis CC, Studentova V, Izdebski R, Oikonomou O, Pfeifer Y, Petinaki E, Hrabak J. 2015. Matrix-assisted laser desorption ionizationtime of flight mass spectrometry meropenem hydrolysis assay with NH4HCO3, a reliable tool for direct detection of carbapenemase activity. J Clin Microbiol 53:1731-1735. https://doi.org/10.1128/JCM.03094-14.
8. Glupczynski Y, Evrard S, Ote I, Mertens P, Huang TD, Leclipteux T, Bogaerts P. 2016. Evaluation of two new commercial immunochromatographic assays for the rapid detection of OXA-48 and KPC carbapenemases from cultured bacteria. J Antimicrob Chemother 71:1217-1222. https://doi.org/10.1093/jac/dkv472.
9. Aktas E, Malkocoglu G, Otlu B, Copur Cicek A, Kulah C, Comert F, Sandalli C, Gursoy NC, Erdemir D, Bulut ME. 30 August 2016. Evaluation of the carbapenem inactivation method for detection of carbapenemaseproducing Gram-negative bacteria in comparison with the Rapidec Carba NP. Microb Drug Resist. Epub ahead of print.
10. Clinical and Laboratory Standards Institute. 2017. Performance standards for antimicrobial susceptibility testing, 27th ed. CLSI supplement M100S. CLSI, Wayne, PA.
11. Pires J, Novais A, Peixe L. 2013. Blue-carba, an easy biochemical test for detection of diverse carbapenemase producers directly from bacterial cultures. J Clin Microbiol 51:4281-4283. https://doi.org/10.1128/JCM.01634-13.
12. Bakour S, Garcia V, Loucif L, Brunel JM, Gharout-Sait A, Touati A, Rolain JM. 2015. Rapid identification of carbapenemase-producing Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii using a modified Carba NP test. New Microbes New Infect 7:89-93. https://doi.org/10.1016/j.nmni.2015.07.001.
13. Doi Y, Potoski BA, Adams-Haduch JM, Sidjabat HE, Pasculle AW, Paterson DL. 2008. Simple disk-based method for detection of Klebsiella pneumoniae carbapenemase-type beta-lactamase by use of a boronic acid compound. J Clin Microbiol 46:4083-4086. https://doi.org/10.1128/JCM.01408-08.
14. Lutgring JD, Limbago BM. 2016. The problem of carbapenemaseproducing-carbapenem-resistant-enterobacteriaceae detection. J Clin Microbiol 54:529-534. https://doi.org/10.1128/JCM.02771-15.
15. Viau R, Frank KM, Jacobs MR, Wilson B, Kaye K, Donskey CJ, Perez F, Endimiani A, Bonomo RA. 2016. Intestinal carriage of carbapenemaseproducing organisms: current status of surveillance methods. Clin Microbiol Rev 29:1-27. https://doi.org/10.1128/CMR.00108-14.
16. Simner PJ, Gilmour MW, DeGagne P, Nichol K, Karlowsky JA. 2015. Evaluation of five chromogenic agar media and the Rosco Rapid Carb screen kit for detection and confirmation of carbapenemase production in Gram-negative bacilli. J Clin Microbiol 53:105-112. https://doi.org/10.1128/JCM.02068-14.
17. Vasoo S, Cunningham SA, Kohner PC, Simner PJ, Mandrekar JN, Lolans K, Hayden MK, Patel R. 2013. Comparison of a novel, rapid chromogenic biochemical assay, the Carba NP test, with the modified Hodge test for detection of carbapenemase-producing Gram-negative bacilli. J Clin Microbiol 51:3097-3101. https://doi.org/10.1128/JCM.00965-13.
18. Tijet N, Patel SN, Melano RG. 2016. Detection of carbapenemase activity in Enterobacteriaceae: comparison of the carbapenem inactivation method versus the Carba NP test. J Antimicrob Chemother 71:274-276. https://doi.org/10.1093/jac/dkv283.
19. Yamada K, Kashiwa M, Arai K, Nagano N, Saito R. 2016. Comparison of the modified-Hodge test, Carba NP test, and carbapenem inactivation method as screening methods for carbapenemase-producing Enterobacteriaceae. J Microbiol Methods 128:48-51. https://doi.org/10.1016/j.mimet.2016.06.019.
20. Zhanel GG, DeCorby M, Laing N, Weshnoweski B, Vashisht R, Tailor F, Nichol KA, Wierzbowski A, Baudry PJ, Karlowsky JA, Lagace-Wiens P, Walkty A, McCracken M, Mulvey MR, Johnson J, Canadian Antimicrobial Resistance Alliance, Hoban DJ. 2008. Antimicrobial-resistant pathogens in intensive care units in Canada: results of the Canadian National Intensive Care Unit (CAN-ICU) study, 2005-2006. Antimicrob Agents Chemother 52:1430-1437. https://doi.org/10.1128/AAC.01538-07.
21. Taylor F, Baudry P, Zhanel GG, Hoban DJ. Emergence of Escherichia coli and Klebsiella pneumoniae with reduced susceptibilities to carbapenems in Canadian hospitals. Proceedings of the Canadian Association of Clinical Microbiology and Infectious Diseases Annual Meeting. Canadian Association of Clinical Microbiology and Infectious Diseases Annual Meeting, Toronto, ON, Canada.