CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis

Science China Life Sciences

Volumn 60, Issue 5, 2017, Pages 520-523

  Chen, Yiyu ^a   Wang, Zhiping ^a   Ni, Hanwen ^a   Xu, Yong ^b   Chen, Qijun ^a   Jiang, Linjian ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b BEIJING VEGETABLE RESEARCH CENTER   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ARABIDOPSIS PROTEIN; PLANT DNA;

AMINO ACID SEQUENCE; ARABIDOPSIS; CRISPR CAS SYSTEM; GAIN OF FUNCTION MUTATION; GENE EDITING; GENETICS; NUCLEOTIDE SEQUENCE; PROCEDURES; SEQUENCE HOMOLOGY; TRANSGENIC PLANT;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARABIDOPSIS PROTEINS; BASE SEQUENCE; CRISPR-CAS SYSTEMS; DNA, PLANT; GAIN OF FUNCTION MUTATION; GENE EDITING; PLANTS, GENETICALLY MODIFIED; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID;

EID: 85015626714     PISSN: 16747305     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11427-017-9021-5     Document Type: Letter

References (5)

1. Komor, A.C., Kim, Y.B., Packer, M.S., Zuris, J.A., and Liu, D. (2016). Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature 533, 420–424.
2. Li, J., Sun, Y., Du, J., Zhao, Y., and Xia, L. (2017). Generation of targeted point mutations in rice by a modified CRISPR/Cas9 system. Mol Plant 10, 526–529.
3. Lu, Y., and Zhu, J.K. (2017). Precise editing of a target base in the rice genome using a modified CRISPR/Cas9 system. Mol Plant, 10, 523–526.
4. Ren, B., Yan, F., Kuang, Y., Li, N., Zhang, D., Lin, H., and Zhou, H. (2017). A CRISPR/Cas9 toolkit for efficient targeted base editing to induce genetic variations in rice. Sci China Life Sci, doi: 10.1007/s11427-016-0406-x.
5. Wang, Z.P., Xing, H.L., Dong, L., Zhang, H.Y., Han, C.Y., Wang, X.C., and Chen, Q.J. (2015). Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol 16, 144.