Scanning electrochemical microscopy of living cells

Scanning Electrochemical Microscopy, Second Edition

Volumn , Issue , 2012, Pages 379-415

  Mauzeroll, Janine ^a   Schougaard, Steen B ^a  

^a UNIVERSITÉ DU QUÉBEC À MONTRÉAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 85014818573     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1201/b11850     Document Type: Chapter

References (106)

1. Adams, R. N. Probing brain chemistry with electroanalytical techniques. Anal Chem 1976, 48, 1126A-1138A.
2. Wightman, R. M., Jankowski, J. A., Kennedy, R. T., Cowage, K. T., Schroeder, T. J., Leszczyszyn, D. J., Near, J. A., Diliberto, E. J., Viveros, O. H. Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells. Proc Natl Acad Sci USA 1991, 88, 10754-10758.
3. Wightman, R. M., Kennedy, R. T., Wiedemann, D. J., Kawagoe, K. T., Zimmerman, J. B., Leszczyszyn, D. J. Biological systems. In Microelectrodes: Theory and Applications. Montenegro, M. I., Queiros, M. A., Daschbach, J. L. (Eds.). Kluwer Academic Publishing, Dordrecht, the Netherlands, 1991, pp. 453-462.
4. Cannon, D. M., Jr., Winograd, N., Ewing, A. G. Quantitative chemical analysis of single cells. Annu Rev Biophys Biomol Struct 2000, 29, 239-263.
5. Ewing, A. G., Strein, T. S., Lau, Y. Y. Analytical chemistry in microenvironments: Single nerve cells. Acc Chem Res 1992, 25, 440-447.
6. Kennedy, R. T., Huang, L., Atkinson, M. A., Dush, P. Amperometric monitoring of individual exocytosis events in pancreatic beta cells. Anal Chem 1993, 65, 1882.
7. Pantano, P., Kuhr, W. G. Enzyme-modified microelectrodes for in vivo neurochemical measurements. Electroanalysis 1995, 7, 405-416.
8. Lu, H., Gratzl, M. Monitoring drug efflux from sensitive and multidrug-resistant single cancer cells with microvoltammetry. Anal Chem 1999, 71, 2821-2830.
9. Pace, N. R. The molecular tree of life changes how we see, teach microbial diversity. Microbe 2008, 3, 15-20.
10. Sapp, J. The new foundations of evolution on the tree of life. New York: Oxford University Press, 2009.
11. Lee, C., Kwak, J., Bard, A. J. Application of scanning electrochemical microscopy to biological samples. Proc Natl Acad Sci USA 1990, 87, 1740-1743.
12. Amemiya, S., Bard, A. J., Fan, F.-R. F., Mirkin, M. V., Unwin, P. R. Scanning electrochemical microscopy. Annu Rev Anal Chem 2008, 1, 95-131.
13. Bard, A. J., Li, X., Zhan, W. Chemically imaging living cells by scanning electrochemical microscopy. Biosens Bioelectron 2006, 22, 461-472.
14. Edwards, M. A., Martin, S., Whitworth, A. L., Macpherson, J. V., Unwin, P. R. Scanning electrochemical microscopy: Principles and applications to biophysical systems. Physiol Meas 2006, 27, R63-R108.
15. Schulte, A., Schuhmann, W. Single-cell microelectrochemistry. Angew Chem Int Ed 2007, 46, 8760-8777.
16. Wittstock, G., Burchardt, M., Pust, S. E., Shen, Y., Zhao, C. Scanning electrochemical microscopy for direct imaging of reaction rates. Angew Chem Int Ed 2007, 46, 1584-1617.
17. Wittstock, G., Wilhelm, T., Bahrs, S., Steinrücke, P. SECM feedback imaging of enzymatic activity on agglomerated microbeads. Electroanalysis 2001, 13, 669-675.
18. Amemiya, S., Guo, J., Xiong, H., Gross, D. A. Biological applications of scanning electrochemical microscopy: Chemical imaging of single living cells and beyond. Anal Bioanal Chem 2006, 386, 458-471.
19. Sun, P., Laforge, F. O., Mirkin, M. V. Scanning electrochemical microscopy in the 21st century. PCCP 2007, 9, 802-823.
20. Schulte, A., Nebel, M., Schuhmann, W. Scanning electrochemical microscopy in neuroscience. Annu Rev Anal Chem 2010, 3, 299-318.
21. Yasukawa, T., Kaya, T., Matsue, T. Characterization and imaging of single cells with scanning electrochemical microscopy. Electroanalysis 2000, 12, 653-659.
22. Wang, W., Zhang, S.-H., Li, L.-M., Wang, Z.-L., Cheng, J.-K., Huang, W.-H. Monitoring of vesicular exocytosis from single cells using micrometer and nanometer-sized electrochemical sensors. Anal Bioanal Chem 2009, 394, 17-32.
23. Roberts, W. S., Lonsdale, D. J., Griffiths, J., Higsona, S. P. J. Advances in the application of scanning electrochemical microscopy to bioanalytical systems. Biosens Bioelectron 2007, 23, 301-318.
24. Yotter, R. A., Wilson, D. M. Sensor technologies for monitoring metabolic activity in single cells—Part II: Nonoptical methods and applications. IEEE Sens J 2004, 4, 412-429.
25. Beaulieu, I., Kuss, S., Mauzeroll, J., Geissler, M. Biological scanning electrochemical microscopy and its application to live cell studies. Anal Chem 2011, 83, 1485-1492.
26. Shiku, H., Ohya, H., Matsue, T. Scanning electrochemical microscopy applied to biological systems. In Encyclopedia of Electrochemistry. Bard, A. J., Stratmann, M. (Eds.). Wiley-VCH, Weinheim, Germany, 2001, pp. 257-275.
27. Horrocks, B. R., Wittstock, G. Biological systems. In Scanning Electrochemical Microscopy. Bard, A. J., Mirkin, M. V. (Eds.). Marcel Dekker, New York, 2000, pp. 445-519.
28. Nagamine, K., Kaya, T., Yasukawa, T., Shiku, H., Matsue, T. Application of microbial chip for amperometric detection of metabolic alteration in bacteria. Sens Actuators B Chem 2005, 108, 676-682.
29. Cai, C., Liu, B., Mirkin, M. V., Frank, H. A., Rusling, J. F. Scanning electrochemical microscopy of living cells. 3. Rhodobacter sphaeroides. Anal Chem 2002, 74, 114-119.
30. Nagamine, K., Matsui, N., Kaya, T., Yasukawa, T., Shiku, H., Nakayama, T., Nishino, T., Matsue, T. Amperometric detection of the bacterial metabolic regulation with a microbial array chip. Biosens Bioelectron 2005, 21, 145-151.
31. Matsui, N., Kaya, T., Nagamine, K., Yasukawa, T., Shiku, H., Matsue, T. Electrochemical mutagen screening using microbial chip. Biosens Bioelectron 2006, 21, 1202-1209.
32. Kaya, T., Nishizawa, M., Yasukawa, T., Nishiguchi, M., Onouchi, T., Matsue, T. A microbial chip combined with scanning electrochemical microscopy. Biotechnol Bioeng 2001, 76, 391-394.
33. Holt, K. B., Bard, A. J. Interaction of silver(I) ions with the respiratory chain of Escherichia coli: An electrochemical and scanning electrochemical microscopy study of the antimicrobial mechanism of micromolar Ag+. Biochemistry 2005, 44, 13214-13223.
34. Kaya, T., Nagamine, K., Oyamatsu, D., Shiku, H., Nishizawa, M., Matsue, T. Fabrication of microbial chip using collagen gel microstructure. Lab Chip 2003, 3, 313-317.
35. Kaya, T., Nagamine, K., Oyamatsu, D., Nishizawa, M., Matsue, T. A microbial chip for glucose sensing studied with scanning electrochemical microscopy (SECM). Electrochemistry 2003, 71, 436-438.
36. Kaya, T., Numai, D., Nagamine, K., Aoyagi, S., Shiku, H., Matsue, T. Respiration activity of Escherichia coli entrapped in a cone-shaped microwell and cylindrical micropore monitored by scanning electrochemical microscopy (SECM). Analyst 2004, 129, 529-534.
37. Nagamine, K., Onodera, S., Torisawa, Y.-S., Yasukawa, T., Shiku, H., Matsue, T. On-chip transformation of bacteria. Anal Chem 2005, 77, 4278-4281.
38. Nagamine, K., Onodera, S., Kurihara, A., Yasukawa, T., Shiku, H., Asano, R., Kumagai, I., Matsue, T. Electrochemical screening of recombinant protein solubility in Escherichia coli using scanning electrochemical microscopy (SECM). Biotechnol Bioeng 2007, 96, 1008-1013.
39. Zhan, D., Fan, F.-R. F., Bard, A. J. The Kv channel blocker 4-aminopyridine enhances Ag+ uptake: A scanning electrochemical microscopy study of single living cells. Proc Natl Acad Sci USA 2008, 105, 12118-12122.
40. Takahashi, Y., Hirano, Y., Yasukawa, T., Shiku, H., Yamada, H., Matsue, T. Topographic, electrochemical, and optical images captured using standing approach mode scanning electrochemical/optical microscopy. Langmuir 2006, 22, 10299-10306.
41. Yasukawa, T., Matsue, T. Characterization of single cells using microelectrode systems. Electrochemistry 1999, 67, 264-268.
42. Matsue, T., Koike, S., Uchida, I. Microamperometric estimation of photosynthesis inhibition in a single algal protoplast. Biochem Biophys Res Commun 1993, 197, 1283-1287.
43. Guo, J., Amemiya, S. Permeability of the nuclear envelope at isolated Xenopus oocyte nuclei studied by scanning electrochemical microscopy. Anal Chem 2005, 77, 2147-2156.
44. Sun, P., Laforge, F. O., Abeyweera, T. P., Rotenberg, S. A., Carpino, J., Mirkin, M. V. Nanoelectrochemistry of mammalian cells. Proc Natl Acad Sci USA 2008, 105, 443-448.
45. Gao, N., Wang, X., Li, L., Zhang, X., Jin, W. Scanning electrochemical microscopy coupled with intracellular standard addition method for quantification of enzyme activity in single intact cells. Analyst 2007, 132, 1139-1146.
46. Tsionsky, M., Cardon, Z. G., Bard, A. J., Jackson, R. B. Photosynthetic electron transport in single guard cells as measured by scanning electrochemical microscopy. Plant Physiol 1997, 113, 895-901.
47. Yasukawa, T., Uchida, I., Matsue, T. Permeation of redox species through a cell membrane of a single, living algal protoplast studied by microamperometry. Biochim Biophys Acta Biomembr 1998, 1369, 152-158.
48. Yasukawa, T., Kaya, T., Matsue, T. Dual imaging of topography and photosynthetic activity of a single protoplast by scanning electrochemical microscopy. Anal Chem 1999, 71, 4637-4641.
49. Yasukawa, T., Uchida, I., Matsue, T. Microamperometric measurements of photosynthetic activity in a single algal protoplast. Biophys J 1999, 76, 1129-1135.
50. Hengstenberg, A., Blöchl, A., Dietzel, I. D., Schuhmann, W. Spatially resolved detection of neurotransmitter secretion from individual cells by means of scanning electrochemical microscopy. Angew Chem Int Ed 2001, 40, 905-908.
51. Ciobanu, M., Taylor, D. E., Jr., Wilburn, J. P., Cliffel, D. E. Glucose and lactate biosensors for scanning electrochemical microscopy imaging of single live cells. Anal Chem 2008, 80, 2717-2727.
52. Schrock, D. S., Baur, J. E. Chemical imaging with combined fast-scan cyclic voltammetry-scanning electrochemical microscopy. Anal Chem 2007, 79, 7053-7061.
53. Takahashi, Y., Miyamoto, T., Shiku, H., Asano, R., Yasukawa, T., Kumagai, I., Matsue, T. Electrochemical detection of epidermal growth factor receptors on a single living cell surface by scanning electrochemical microscopy. Anal Chem 2009, 81, 2785-2790.
54. Pitta Bauermann, L., Schuhmann, W., Schulte, A. An advanced biological scanning electrochemical microscope (Bio-SECM) for studying individual living cells. PCCP 2004, 6, 4003-4008.
55. Zhao, X., Petersen, N. O., Ding, Z. Comparison study of live cells by atomic force microscopy, confocal microscopy, and scanning electrochemical microscopy. Can J Chem 2007, 85, 175-183.
56. Diakowski, P. M., Ding, Z. Interrogation of living cells using alternating current scanning electrochemical microscopy (AC-SECM). PCCP 2007, 9, 5966-5974.
57. Berger, C. E. M., Rathod, H., Gillespie, J. I., Horrocks, B. R., Datta, H. K. Scanning electrochemical microscopy at the surface of bone-resorbing osteoclasts: Evidence for steady-state disposal and intracellular functional compartmentalization of calcium. J Bone Miner Res 2001, 16, 2092-2102.
58. Ueda, A., Niwa, O., Maruyama, K., Shindo, Y., Oka, K., Suzuki, K. Neurite imaging of living PC12 cells with scanning electrochemical/near-field optical/atomic force microscopy. Angew Chem Int Ed 2007, 46, 8238-8241.
59. Maruyama, K., Ohkawa, H., Ogawa, S., Ueda, A., Niwa, O., Suzuki, K. Fabrication and characterization of a nanometer-sized optical fiber electrode based on selective chemical etching for scanning electrochemical/optical microscopy. Anal Chem 2006, 78, 1904-1912.
60. Takii, Y., Takoh, K., Nishizawa, M., Matsue, T. Characterization of local respiratory activity of PC12 neuronal cell by scanning electrochemical microscopy. Electrochim Acta 2003, 48, 3381-3385.
61. Kurulugama, R. T., Wipf, D. O., Takacs, S. A., Pongmayteegul, S., Garris, P. A., Baur, J. E. Scanning electrochemical microscopy of model neurons: Constant distance imaging. Anal Chem 2005, 77, 1111-1117.
62. Liebetrau, J. M., Miller, H. M., Baur, J. E., Takacs, S. A., Anupunpisit, V., Garris, P. A., Wipf, D. O. Scanning electrochemical microscopy of model neurons: Imaging and real-time detection of morphological changes. Anal Chem 2003, 75, 563-571.
63. Zhu, L., Gao, N., Zhang, X., Jin, W. Accurately measuring respiratory activity of single living cells by scanning electrochemical microscopy. Talanta 2008, 77, 804-808.
64. Xue, Y., Ding, L., Lei, J., Yan, F., Ju, H. In situ electrochemical imaging of membrane glycan expression on micropatterned adherent single cells. Anal Chem 2010, 82, 7112-7118.
65. Zhao, X., Lam, S., Jass, J., Ding, Z. Scanning electrochemical microscopy of single human urinary bladder cells using reactive oxygen species as probe of inflammatory response. Electrochem Commun 2010, 12, 773-776.
66. Yasukawa, T., Kondo, Y., Uchida, I., Matsue, T. Imaging of cellular activity of single cultured cells by scanning electrochemical microscopy. Chem Lett 1998, 8, 767-768.
67. Wang, W., Xiong, Y., Du, F.-Y., Huang, W.-H., Wu, W.-Z., Wang, Z.-L., Cheng, J.-K., Yang, Y.-F. Imaging and detection of morphological changes of single cells before and after secretion using scanning electrochemical microscopy. Analyst 2007, 132, 515-518.
68. Isik, S., Schuhmann, W. Detection of nitric oxide release from single cells by using constant-distance-mode scanning electrochemical microscopy. Angew Chem Int Ed 2006, 45, 7451-7454.
69. Mauzeroll, J., Bard, A. J., Owhadian, O., Monks, T. J. Menadione metabolism to thiodione in hepatoblastoma by scanning electrochemical microscopy. Proc Natl Acad Sci USA 2004, 101, 17582-17587.
70. Hirano, Y., Nishimiya, Y., Kowata, K., Mizutani, F., Tsuda, S., Komatsu, Y. Construction of time-lapse scanning electrochemical microscopy with temperature control and its application to evaluate the preservation effects of antifreeze proteins on living cells. Anal Chem 2008, 80, 9349-9354.
71. Takahashi, Y., Shiku, H., Murata, T., Yasukawa, T., Matsue, T. Transfected single-cell imaging by scanning electrochemical optical microscopy with shear force feedback regulation. Anal Chem 2009, 81, 9674-9681.
72. Kaya, T., Torisawa, Y.-S., Oyamatsu, D., Nishizawa, M., Matsue, T. Monitoring the cellular activity of a cultured single cell by scanning electrochemical microscopy (SECM). A comparison with fluorescence viability monitoring. Biosens Bioelectron 2003, 18, 1379-1383.
73. Nishizawa, M., Takoh, K., Matsue, T. Micropatterning of HeLa cells on glass substrates and evaluation of respiratory activity using microelectrodes. Langmuir 2002, 18, 3645-3649.
74. Murata, T., Yasukawa, T., Shiku, H., Matsue, T. Electrochemical single-cell gene-expression assay combining dielectrophoretic manipulation with secreted alkaline phosphatase reporter system. Biosens Bioelectron 2009, 25, 913-919.
75. Shiku, H., Takeda, M., Murata, T., Akiba, U., Hamada, F., Matsue, T. Development of electrochemical reporter assay using HeLa cells transfected with vector plasmids encoding various responsive elements. Anal Chim Acta 2009, 640, 87-92.
76. Chen, Z., Xie, S., Shen, L., Du, Y., He, S., Li, Q., Liang, Z., Meng, X., Li, B., Xu, X., Ma, H., Huang, Y., Shao, Y. Investigation of the interactions between silver nanoparticles and HeLa cells by scanning electrochemical microscopy. Analyst 2008, 133, 1221-1228.
77. Li, X., Bard, A. J. Scanning electrochemical microscopy of HeLa cells—Effects of ferrocene methanol and silver ion. J Electroanal Chem 2009, 628, 35-42.
78. Koley, D., Bard, A. J. Triton X-100 concentration effects on membrane permeability of a single HeLa cell by scanning electrochemical microscopy (SECM). Proc Natl Acad Sci USA 2010, 107, 16783-16787.
79. Liu, B., Rotenberg, S. A., Mirkin, M. V. Scanning electrochemical microscopy of living cells: Different redox activities of nonmetastatic and metastatic human breast cells. Proc Natl Acad Sci USA 2000, 97, 9855-9860.
80. Mirkin, M. V., Liu, B., Rotenberg, S. A. Probing redox activity of human breast cells by scanning electrochemical microscopy. In Methods in Enzymology. Abelson, J. N., Simon, M. I. (Eds.). Academic Press, 2002, pp. 112-122.
81. Rotenberg, S. A., Mirkin, M. V. Scanning electrochemical microscopy: Detection of human breast cancer cells by redox environment. J Mammary Gland Biol Neoplasia 2004, 9, 375-382.
82. Feng, W., Rotenberg, S. A., Mirkin, M. V. Scanning electrochemical microscopy of living cells. 5. Imaging of fields of normal and metastatic human breast cells. Anal Chem 2003, 75, 4148-4154.
83. Liu, B., Rotenberg, S. A., Mirkin, M. V. Scanning electrochemical microscopy of living cells. 4. Mechanistic study of charge transfer reactions in human breast cells. Anal Chem 2002, 74, 6340-6348.
84. Liu, B., Cheng, W., Rotenberg, S. A., Mirkin, M. V. Scanning electrochemical microscopy of living cells: Part 2. Imaging redox and acid/basic reactivities. J Electroanal Chem 2001, 500, 590-597.
85. Mauzeroll, J., Bard, A. J. Scanning electrochemical microscopy of menadione-glutathione conjugate export from yeast cells. Proc Natl Acad Sci USA 2004, 101, 7862-7867.
86. Shiku, H., Torisawa, Y.-S., Takagi, A., Aoyagi, S., Abe, H., Hoshi, H., Yasukawa, T., Matsue, T. Metabolic and enzymatic activities of individual cells, spheroids and embryos as a function of the sample size. Sens Actuators B Chem 2005, 108, 597-602.
87. Garay, M. F., Ufheil, J., Borgwarth, K., Heinze, J. Retrospective chemical analysis of tree rings by means of the scanning electrochemical microscopy with shear force feedback. PCCP 2004, 6, 4028-4033.
88. Zhu, R., Macfie, S. M., Ding, Z. Cadmium-induced plant stress investigated by scanning electrochemical microscopy. J Exp Bot 2005, 56, 2831-2838.
89. Parthasarathy, M., Singh, S., Hazra, S., Pillai, V. K. Imaging the stomatal physiology of somatic embryo-derived peanut leaves by scanning electrochemical microscopy. Anal Bioanal Chem 2008, 391, 2227-2233.
90. Ktari, N., Poncet, P., Snchal, H., Malaquin, L., Kanoufi, F., Combellas, C. Patterning of polystyrene by scanning electrochemical microscopy. Biological applications to cell adhesion. Langmuir 2010, 26, 17348-17356.
91. Shiku, H., Shiraishi, T., Aoyagi, S., Utsumi, Y., Matsudaira, M., Abe, H., Hoshi, H., Kasai, S., Ohya, H., Matsue, T. Respiration activity of single bovine embryos entrapped in a cone-shaped microwell monitored by scanning electrochemical microscopy. Anal Chim Acta 2004, 522, 51-58.
92. Shiku, H., Shiraishi, T., Ohya, H., Matsue, T., Abe, H., Hoshi, H., Kobayashi, M. Oxygen consumption of single bovine embryos probed by scanning electrochemical microscopy. Anal Chem 2001, 73, 3751-3758.
93. Aoyagi, S., Utsumi, Y., Masudaira, M., Yamada, H., Kaachi, M., Shiku, H., Abe, H., Hoshi, H., Matsue, T. Quality Evaluation of in vitro-produced bovine embryos by respiration measurement and development of semi-automatic instrument. Bunseki Kagaku 2006, 55, 847-854.
94. Agung, B., Otoi, T., Abe, H., Hoshi, H., Murakami, M., Karja, N. W. K., Murakami, M. K., Wongsrikeao, P., Watari, H., Suzuki, T. Relationship between oxygen consumption and sex of bovine in vitro fertilized embryos. Reprod Domest Anim 2005, 40, 51-56.
95. Abe, H., Hoshi, H. Evaluation of bovine embryos produced in high performance serum-free media. J Reprod Dev 2003, 49, 193-202.
96. Goto, M., Abe, H., Ito-Sasaki, T., Goto, M., Inagaki, A., Ogawa, N., Fujimori, K., Kurokawa, Y., Matsue, T., Satomi, S. A novel predictive method for assessing the quality of isolated pancreatic islets using scanning electrochemical microscopy. Transplant Proc 2009, 41, 311-313.
97. Torisawa, Y.-S., Kaya, T., Takii, Y., Oyamatsu, D., Nishizawa, M., Matsue, T. Scanning electrochemical microscopy-based drug sensitivity test for a cell culture integrated in silicon microstructures. Anal Chem 2003, 75, 2154-2158.
98. Torisawa, Y.-S., Shiku, H., Kasai, S., Nishizawa, M., Matsue, T. Proliferation assay on a silicon chip applicable for tumors extirpated from mammalians. Int J Cancer 2004, 109, 302-308.
99. Zhan, D., Li, X., Zhan, W., Fan, F.-R. F., Bard, A. J. Scanning electrochemical microscopy. 58. Application of a micropipet-supported ITIES tip to detect Ag+ and study its effect on fibroblast cells. Anal Chem 2007, 79, 5225-5231.
100. Takoh, K., Takahashi, A., Matsue, T., Nishizawa, M. A porous membrane-based microelectroanalytical technique for evaluating locally stimulated culture cells. Anal Chim Acta 2004, 522, 45-49.
101. Kaji, H., Kanada, M., Oyamatsu, D., Matsue, T., Nishizawa, M. Microelectrochemical approach to induce local cell adhesion and growth on substrates. Langmuir 2004, 20, 16-19.
102. Torisawa, Y.-S., Shiku, H., Yasukawa, T., Nishizawa, M., Matsue, T. Three-dimensional micro-culture system with a silicon-based cell array device for multi-channel drug sensitivity test. Sens Actuators B Chem 2005, 108, 654-659.
103. Torisawa, Y.-S., Shiku, H., Yasukawa, T., Nishizawa, M., Matsue, T. Multi-channel 3-D cell culture device integrated on a silicon chip for anticancer drug sensitivity test. Biomaterials 2005, 26, 2165-2172.
104. Pailleret, A., Oni, J., Reiter, S., Isik, S., Etienne, M., Bedioui, F., Schuhmann, W. In situ formation and scanning electrochemical microscopy assisted positioning of NO-sensors above human umbilical vein endothelial cells for the detection of nitric oxide release. Electrochem Commun 2003, 5, 847-852.
105. Borgmann, S., Radtke, I., Erichsen, T., Blöchl, A., Heumann, R., Schuhmann, W. Electrochemical high-content screening of nitric oxide release from endothelial cells. ChemBioChem 2006, 7, 662-668.
106. Isik, S., Etienne, M., Oni, J., Blöchl, A., Reiter, S., Schuhmann, W. Dual microelectrodes for distance control and detection of nitric oxide from endothelial cells by means of scanning electrochemical microscope. Anal Chem 2004, 76, 6389-6394.