Quantitative comparison of human intestinal mononuclear leukocyte isolation techniques for flow cytometric analyses

Journal of Immunological Methods

Volumn 445, Issue , 2017, Pages 45-52

  Schreurs, R R C E ^a,b   Drewniak, A ^b   Bakx, R ^c   Corpeleijn, W E ^a   Geijtenbeek, T H B ^b   van Goudoever, J B ^a   Bunders, M J ^a,b  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b ACADEMIC MEDICAL CENTER   (Netherlands)

^c EMMA CHILDREN S HOSPITAL   (Netherlands)

Author keywords

Human; Intestine; Isolation method; Lymphocytes; Monocytes

Indexed keywords

CD28 ANTIGEN; CD4 ANTIGEN; CD4 RECEPTOR; CD56 ANTIGEN; COLLAGENASE; COLLAGENASE D; LIBERASE; LIBERASE TL; RECEPTOR; UNCLASSIFIED DRUG; THERMOLYSIN;

ADULT; ARTICLE; CELL ISOLATION; CELL SUSPENSION; CELL VIABILITY; CONTROLLED STUDY; DISSOCIATION; ENZYME DEGRADATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN TISSUE; INFANT; INTESTINE; LAMINA PROPRIA; MONOCYTE; MONONUCLEAR CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; T LYMPHOCYTE; COMPARATIVE STUDY; CYTOLOGY; IMMUNOLOGY; METABOLISM;

COLLAGENASES; FLOW CYTOMETRY; HUMANS; INTESTINES; LEUKOCYTES, MONONUCLEAR; THERMOLYSIN;

EID: 85014788266     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2017.03.006     Document Type: Article

References (24)

1. Annunziato, F., Cosmi, L., Santarlasci, V., Maggi, L., Liotta, F., Mazzinghi, B.,.. Romagnani, S., Phenotypic and functional features of human Th17 cells. J. Exp. Med. 204:8 (2007), 1849–1861, 10.1084/jem.20070663.
2. Braunstein, J., Qiao, L., Autschbach, F., Schürmann, G., Meuer, S., T cells of the human intestinal lamina propria are high producers of interleukin-10. Gut 41:2 (1997), 215–220, 10.1136/gut.41.2.215.
3. Brown, E.M., Sadarangani, M., Finlay, B.B., The role of the immune system in governing host-microbe interactions in the intestine. Nat. Immunol. 14:7 (2013), 660–667, 10.1038/ni.2611.
4. Bull, D.M., Bookman, M.A., Isolation and functional characterization of human intestinal mucosal lymphoid cells. J. Clin. Invest. 59 (1977), 966–974.
5. Bunders, M.J., van der Loos, C.M., Klarenbeek, P.L., van Hamme, J.L., Boer, K., Wilde, J.C.H.,.. Kuijpers, T.W., Memory CD4 + CCR5 + T cells are abundantly present in the gut of newborn infants to facilitate mother-to-child transmission of HIV-1. Blood 120 (2012), 4383–4390, 10.1182/blood-2012-06-437566.
6. Cebra, J.J., Influences of microbiota on intestinal immune system development. Am. J. Clin. Nutr. 69:5 (1999), 1046–1051.
7. Chen, Z., Chen, X., Xu, Y., Xiong, P., Fang, M., Tan, Z.,.. Zheng, F., Collagenase digestion down-regulates the density of CD27 on lymphocytes. J. Immunol. Methods 413 (2014), 57–61, 10.1016/j.jim.2014.06.017.
8. Comer, G.M., Ramey, W.G., Kotler, D.P., Holt, P.R., Isolation of intestinal mononuclear cells from colonoscopic biopsies for immunofluorescence analysis by flow cytometry. Dig. Dis. Sci. 31:2 Supplement (1986), 151–156, 10.1007/BF01300700.
9. Ebert, E.C., Roberts, A.I., Pitfalls in the characterization of small intestinal lymphocytes. J. Immunol. Methods, 178(94), 1995, 219.
10. Fuss, I.J., Kanof, M.E., Smith, P.D., Zola, H., Isolation of whole mononuclear cells from peripheral blood and cord blood. Curr. Protoc. Immunol. 85 (2009), 7.1.1–7.1.8.
11. Kunkel, E.J., Butcher, E.C., Chemokines and the tissue-specific migration of lymphocytes. Immunity 16:1 (2002), 1–4, 10.1016/S1074-7613(01)00261-8.
12. Lundqvist, C., Hammarström, M.L., Athlin, L., Hammarström, S., Isolation of functionally active intraepithelial lymphocytes and enterocytes from human small and large intestine. J. Immunol. Methods 152:2 (1992), 253–263, 10.1016/0022-1759(92)90147-L.
13. Mowat, A.M., Agace, W.W., Regional specialization within the intestinal immune system. Nat. Rev. Immunol. 14:10 (2014), 667–685, 10.1038/nri3738.
14. Mulder, W.M., Koenen, H., van de Muysenberg, A.J., Bloemena, E., Wagstaff, J., Scheper, R.J., Reduced expression of distinct T-cell CD molecules by collegenase/DNAse treatment. Cancer Immunol. Immunother. 38:4 (1994), 253–258.
15. Ryon, J.J., Isolation of mononuclear cells from tonsillar tissue. Curr. Protoc. Immunol. 00:I (2001), 7.8.1–7.8.3.
16. Sathaliyawala, T., Kubota, M., Yudanin, N., Turner, D., Camp, P., Thome, J.J.C.,.. Farber, D.L., Distribution and compartmentalization of human circulating and tissue-resident memory T cell subsets. Immunity 38:1 (2013), 187–197, 10.1016/j.immuni.2012.09.020.
17. Shacklett, B.L., Yang, O., Hausner, M.A., Elliott, J., Hultin, L., Price, C.,.. Jamieson, B.D., Optimization of methods to assess human mucosal T-cell responses to HIV infection. J. Immunol. Methods 279:1–2 (2003), 17–31, 10.1016/S0022-1759(03)00255-2.
18. Shen, C., Xu, H., Alvarez, X., Lackner, A.a., Veazey, R.S., Wang, X., Reduced expression of CD27 by collagenase treatment: implications for interpreting B cell data in tissues. PLoS One, 10(3), 2015, e0116667, 10.1371/journal.pone.0116667.
19. Siddiqui, K.R.R., Powrie, F., CD103 + GALT DCs promote Foxp3 + regulatory T cells. Mucosal Immunol. 1 (November):1 Suppl (2008), S34–S38, 10.1038/mi.2008.43.
20. Thome, J.J.C., Bickham, K.L., Ohmura, Y., Kubota, M., Matsuoka, N., Gordon, C.,.. Farber, D.L., Early-life compartmentalization of human T cell differentiation and regulatory function in mucosal and lymphoid tissues. Nat. Med. 22:1 (2016), 72–77, 10.1038/nm.4008.
21. Troncone, E., Marafini, I., Pallone, F., Monteleone, G., Th17 cytokines in inflammatory bowel diseases: discerning the good from the bad. Int. Rev. Immunol. 32:5–6 (2013), 526–533, 10.3109/08830185.2013.823421.
22. Uronen-Hansson, H., Persson, E., Nilsson, P., Agace, W., Isolation of cells from human intestinal tissue. Bio-protocol, 4(7), 2014, e1092, 10.21769/BioProtoc.1092.
23. Veenbergen, S., Samsom, J.N., Maintenance of small intestinal and colonic tolerance by IL-10-producing regulatory T cell subsets. Curr. Opin. Immunol. 24:3 (2012), 269–276, 10.1016/j.coi.2012.03.004.
24. Wahl, L.M., Smith, P.D., Isolation of macrophages/monocytes from human peripheral blood and tissues. Coligan, J.E., Kruisbeek, A.M., Marguilies, D.H., Shevach, E.M., Strober, W., (eds.) Current Protocols in Immunology, 1991, John Wiley & Sons Inc. New York, New York, USA, 7.6.1–7.6.8.