Therapeutic properties of Scutellaria baicalensis in db/db mice evaluated using Connectivity Map and network pharmacology

Scientific Reports

Volumn 7, Issue , 2017, Pages

  Kim, Bu Yeo ^a   Song, Kwang Hoon ^a   Lim, Chi Yeon ^b   Cho, Su In ^c  

^a Korea Institute of Oriental Medicine (KIOM)   (South Korea)

^b Dongguk University College of Medicine   (South Korea)

^c Pusan National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

PLANT EXTRACT; SCUTELLARIA BAICALENSIS EXTRACT; TRANSCRIPTOME;

ANIMAL; BIOLOGY; CHEMISTRY; DIABETES MELLITUS; DRUG EFFECT; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENE ONTOLOGY; GENE REGULATORY NETWORK; GLUCOSE BLOOD LEVEL; HEP-G2 CELL LINE; HUMAN; LIPID METABOLISM; LIVER; MALE; METABOLISM; MOUSE; PROCEDURES; SCUTELLARIA BAICALENSIS; TRANSGENIC MOUSE;

ANIMALS; BLOOD GLUCOSE; COMPUTATIONAL BIOLOGY; DIABETES MELLITUS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENE ONTOLOGY; GENE REGULATORY NETWORKS; HEP G2 CELLS; HUMANS; LIPID METABOLISM; LIVER; MALE; MICE; MICE, TRANSGENIC; PLANT EXTRACTS; SCUTELLARIA BAICALENSIS; TRANSCRIPTOME;

EID: 85011290404     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep41711     Document Type: Article

References (48)

1. Donato, M. T., Lahoz, A., Castell, J. V. & Gómez-Lechón, M. J. Cell lines: a tool for in vitro drug metabolism studies. Curr. Drug Metab. 9, 1-11 (2008).
2. Ye, F. et al. Quality evaluation of commercial extracts of Scutellaria baicalensis. Nutr. Cancer 49, 217-222 (2004)
3. Ye, F. et al. Anticancer activity of Scutellaria baicalensis and its potential mechanism. J. Altern. Complement. Med. 8, 567-572 (2002).
4. Chang, W. H., Chen, C. H. & Lu, F. J. Different effects of baicalein, baicalin and wogonin on mitochondrial function, glutathione content and cell cycle progression in human hepatoma cell lines. Planta Med. 68, 128-132 (2002).
5. Ye, F. et al. The effect of Scutellaria baicalensis on the signaling network in hepatocellular carcinoma cells. Nutr. Cancer 61, 530-537 (2009).
6. Chang, W. H. et al. Effect of baicalein on apoptosis of the human HepG2 cell line was induced by mitochondrial dysfunction. Planta Med. 68, 302-306 (2002).
7. Song, K. H. et al. Extracts of Scutellaria baicalensis reduced body weight and blood triglyceride in db/db Mice. Phytother. Res. 27, 244-250 (2013).
8. Shang, Y. Z., Miao, H., Cheng, J. J. & Qi, J. M. Effects of amelioration of total flavonoids from stems and leaves of Scutellaria baicalensis Georgi on cognitive deficits, neuronal damage and free radicals disorder induced by cerebral ischemia in rats. Biol. Pharm. Bull. 29, 805-810 (2006).
9. Dong, Q. et al. Scutellaria baicalensis Georgi extract protects against alcohol-induced acute liver injury in mice and affects the mechanism of ER stress. Mol. Med. Rep. 13, 3052-3062 (2016).
10. Lai, C. C. et al. Baicalein, a Component of Scutellaria baicalensis, Attenuates Kidney Injury Induced by Myocardial Ischemia and Reperfusion. Planta Med. 82, 181-189 (2016).
11. Park, H. S. et al. Korean Scutellaria baicalensis Georgi methanol extracts inhibits metastasis via the Forkhead Box M1 activity in hepatocellular carcinoma cells. J. Ethnopharmacol. 155, 847-851 (2014).
12. Lu, K. Scutellarin from Scutellaria baicalensis suppresses adipogenesis by upregulating PPARα in 3T3-L1 cells. J. Nat. Prod. 76, 672-678 (2013).
13. Shin, H. S., Bae, M. J., Jung, S. Y. & Shon, D. H. Preventive effects of skullcap (Scutellaria baicalensis) extract in a mouse model of food allergy. J. Ethnopharmacol. 153, 667-673 (2014).
14. Huan, S. K. et al. Scutellaria baicalensis alleviates cantharidin-induced rat hemorrhagic cystitis through inhibition of cyclooxygenase-2 overexpression. Molecules 17, 6277-6289 (2012).
15. Kim, H. U., Ryu, J. Y., Lee, J. O. & Lee, S. Y. A systems approach to traditional oriental medicine. Nat. Biotechnol. 33, 264-268 (2015).
16. Wang, L. et al. Dissection of mechanisms of Chinese medicinal formula Realgar-Indigo naturalis as an effective treatment for promyelocytic leukemia. Proc. Natl. Acad. Sci. USA 105, 4826-4831 (2008).
17. Li, X. et al. A network pharmacology study of Chinese medicine QiShenYiQi to reveal its underlying multi-compound, multi-target, multi-pathway mode of action. PLoS One 9, e95004, doi: 10.1371/journal.pone.0095004 (2014).
18. Wang, L. et al. Dissecting active ingredients of Chinese medicine by content-weighted ingredient-target network. Mol. Biosyst. 10, 1905-1911 (2014).
19. Subramanian, A. et al. Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc. Natl. Acad. Sci. USA 102, 15545-15550 (2005).
20. Lamb, J. et al. The Connectivity Map: using gene-expression signatures to connect small molecules, genes, and disease. Science 313, 1929-1935 (2006).
21. Kim, B. Y., Lee, J. & Kim, N. S. Helveticoside is a biologically active component of the seed extract of Descurainia sophia and induces reciprocal gene regulation in A549 human lung cancer cells. BMC Genomics 16, 713, doi: 10.1186/s12864-015-1918-1 (2015).
22. Kim, B. Y. et al. Gene Expression Profile of the A549 Human Non-Small Cell Lung Carcinoma Cell Line following Treatment with the Seeds of Descurainia sophia, a Potential Anticancer Drug. Evid. Based Complement. Alternat. Med. 2013, 584604, doi: 10.1155/2013/584604 (2013).
23. Ernst, J. & Bar-Joseph, Z. STEM: a tool for the analysis of short time series gene expression data. BMC Bioinformatics 7, 191 (2006).
24. Misu, H. et al. A liver-derived secretory protein, selenoprotein P, causes insulin resistance. Cell Metab. 12, 483-495 (2010).
25. Pihlajamäki, J. et al. Thyroid hormone-related regulation of gene expression in human fatty liver. J. Clin. Endocrinol. Metab. 94, 3521-3529 (2009).
26. Wang, C. Z. et al. Selective fraction of Scutellaria baicalensis and its chemopreventive effects on MCF-7 human breast cancer cells. Phytomedicine 17, 63-68 (2010).
27. Ye, F. et al. Molecular mechanism of anti-prostate cancer activity of Scutellaria baicalensis extract. Nutr. Cancer 57, 100-110 (2007).
28. Gaire, B. P., Moon, S. K. & Kim, H. Scutellaria baicalensis in stroke management: natures blessing in traditional Eastern medicine. Chin. J. Integr. Med. 20, 712-720 (2014).
29. Wellen, K. E. & Thompson, C. B. A two-way street: reciprocal regulation of metabolism and signaling. Nat. Rev. Mol. Cell Biol. 13, 270-276 (2012).
30. Barth, A. S. et al. Reciprocal regulation of metabolic and signaling pathways. BMC Genomics 11, 197, doi: 10.1186/1471-2164-11- 197 (2010).
31. Zhou, H. & Li, Y. J. Rho kinase inhibitors: potential treatments for diabetes and diabetic complications. Curr. Pharm. Des. 18, 2964-2973 (2012).
32. Lu, Q. et al. RhoA/mDia-1/profilin-1 signaling targets microvascular endothelial dysfunction in diabetic retinopathy. Graefes Arch. Clin. Exp. Ophthalmol. 253, 669-680 (2015).
33. Lu, Q. Y. et al. Involvement of RhoA/ROCK1 signaling pathway in hyperglycemia-induced microvascular endothelial dysfunction in diabetic retinopathy. Int. J. Clin. Exp. Pathol. 7, 7268-7277 (2014).
34. Riches, K. et al. Type 2 diabetes impairs venous, but not arterial smooth muscle cell function: possible role of differential RhoA activity. Cardiovasc. Revasc. Med. 15, 141-148 (2014).
35. Shen, E. et al. Rac1 is required for cardiomyocyte apoptosis during hyperglycemia. Diabetes 58, 2386-2395 (2009).
36. Moore, F. et al. STAT1 is a master regulator of pancreatic {beta}-cell apoptosis and islet inflammation. J. Biol. Chem. 286, 929-941 (2011).
37. Gupte, A. & Mora, S. Activation of the Cbl insulin signaling pathway in cardiac muscle; dysregulation in obesity and diabetes. Biochem. Biophys. Res. Commun. 342, 751-757 (2006).
38. Sebastian, B. M. & Nagy, L. E. Decreased insulin-dependent glucose transport by chronic ethanol feeding is associated with dysregulation of the Cbl/TC10 pathway in rat adipocytes. Am. J. Physiol. Endocrinol. Metab. 289, E1077-1084 (2005).
39. Bolstad, B. M., Irizarry, R. A., Astrand, M. & Speed, T. P. A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 19, 185-193 (2003).
40. Dennis, G. Jr. et al. DAVID: database for annotation, visualization, and integrated discovery. Genome Biol. 4, P3 (2003).
41. Bang, J. et al. Biphasic functional regulation in hippocampus of rat with chronic cerebral hypoperfusion induced by permanent occlusion of bilateral common carotid artery. PLoS One 8, e70093, doi: 10.1371/journal.pone.0070093 (2013).
42. Lee, S. Y. et al. Comparison of pathways associated with hepatitis B- and C-infected hepatocellular carcinoma using pathway-based class discrimination method. Genomics 99, 347-354 (2012).
43. Chatr-Aryamontri, A. et al. The BioGRID interaction database: 2015 update. Nucleic Acids Res. 43, D470-8, doi: 10.1093/nar/ gku1204 (2015).
44. Shannon, P. et al. Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Res. 13, 2498-2504 (2003).
45. Wu, G., Feng, X. & Stein L. A human functional protein interaction network and its application to cancer data analysis. Genome Biol. 11, R53, doi: 10.1186/gb-2010-11-5-r53 (2010).
46. Merico, D. et al. Enrichment map: a network-based method for gene-set enrichment visualization and interpretation. PLoS One 5, e13984, doi: 10.1371/journal.pone.0013984 (2010).
47. Becker, K. G. et al. PubMatrix: a tool for multiplex literature mining. BMC Bioinformatics 4, 61 (2003).
48. Tusher, V. G., Tibshirani, R. & Chu, G. Significance analysis of microarrays applied to the ionizing radiation response. Proc. Natl. Acad. Sci. USA 98, 5116-5121 (2001).