Enhanced heterologous protein productivity by genome reduction in Lactococcus lactis NZ9000

Microbial Cell Factories

Volumn 16, Issue 1, 2017, Pages

  Zhu, Duolong ^a,b   Fu, Yuxin ^a   Liu, Fulu ^a   Xu, Haijin ^a   Saris, Per Erik Joakim ^b   Qiao, Mingqiang ^a  

^a NANKAI UNIVERSITY   (China)

^b UNIVERSITY OF HELSINKI   (Finland)

Author keywords

Chassis; Heterologous; Lactococcus lactis; LecC; Microbial cell factories; Red fluorescent protein

Indexed keywords

ADENOSINE TRIPHOSPHATE; BACTERIAL DNA; BACTERIOCIN; NISIN; RECOMBINANT PROTEIN; RED FLUORESCENT PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; BACTERIAL GROWTH; BACTERIUM MUTANT; BIOLOGICAL TRAIT; BIOMASS; BIOTECHNOLOGY; CELL LEVEL; CONTROLLED STUDY; FERMENTATION; FUNCTIONAL GENOMICS; GENE DELETION; GENETIC TRANSCRIPTION; GENOME REDUCTION; LACTOCOCCUS LACTIS; LECC GENE; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTEIN SYNTHESIS; STRAIN DIFFERENCE; BIOSYNTHESIS; GENETIC ENGINEERING; GENETICS; METABOLISM; PROCEDURES;

GENETIC ENGINEERING; GENOME, BACTERIAL; LACTOCOCCUS LACTIS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS;

EID: 85010188975     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-016-0616-2     Document Type: Article

References (47)

1. Danchin A. Scaling up synthetic biology: do not forget the chassis. FEBS Lett. 2012;586:2129-37.
2. Singh V. Recent advancements in synthetic biology: current status and challenges. Gene. 2014;535:1-11.
3. Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, Voigt CA. Synthetic biology: engineering Escherichia coli to see light. Nature. 2005;438:441-2.
4. Balagadde FK, Song H, Ozaki J, Collins CH, Barnet M, Arnold FH, Quake SR, You L. A synthetic Escherichia coli predator-prey ecosystem. Mol Syst Biol. 2008;4:187.
5. Shen CR, Lan EI, Dekishima Y, Baez A, Cho KM, Liao JC. Driving forces enable high-titer anaerobic 1-butanol synthesis in Escherichia coli. Appl Environ Microbiol. 2011;77:2905-15.
6. Moon TS, Yoon SH, Lanza AM, Roy-Mayhew JD, Prather KL. Production of glucaric acid from a synthetic pathway in recombinant Escherichia coli. Appl Environ Microbiol. 2009;75:589-95.
7. Hashimoto M, Ichimura T, Mizoguchi H, Tanaka K, Fujimitsu K, Keyamura K, Ote T, Yamakawa T, Yamazaki Y, Mori H, et al. Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome. Mol Microbiol. 2005;55:137-49.
8. Petsch D, Anspach FB. Endotoxin removal from protein solutions. J Biotechnol. 2000;76:97-119.
9. Fischer B, Sumner I, Goodenough P. Isolation, renaturation, and formation of disulfide bonds of eukaryotic proteins expressed in Escherichia coli as inclusion bodies. Biotechnol Bioeng. 1993;41:3-13.
10. Martinez-Garcia E, Jatsenko T, Kivisaar M, de Lorenzo V. Freeing Pseudomonas putida KT2440 of its proviral load strengthens endurance to environmental stresses. Environ Microbiol. 2015;17:76-90.
11. Lieder S, Nikel PI, de Lorenzo V, Takors R. Genome reduction boosts heterologous gene expression in Pseudomonas putida. Microb Cell Fact. 2015;14:1.
12. Morimoto T, Kadoya R, Endo K, Tohata M, Sawada K, Liu S, Ozawa T, Kodama T, Kakeshita H, Kageyama Y, et al. Enhanced recombinant protein productivity by genome reduction in Bacillus subtilis. DNA Res. 2008;15:73-81.
13. Unthan S, Baumgart M, Radek A, Herbst M, Siebert D, Bruhl N, Bartsch A, Bott M, Wiechert W, Marin K, et al. Chassis organism from Corynebacterium glutamicum-a top-down approach to identify and delete irrelevant gene clusters. Biotechnol J. 2015;10:290-301.
14. Mierau I, Leij P, van Swam I, Blommestein B, Floris E, Mond J, Smid EJ. Industrial-scale production and purification of a heterologous protein in Lactococcus lactis using the nisin-controlled gene expression system NICE: the case of lysostaphin. Microb Cell Fact. 2005;4:15.
15. Yin JC, Li GX, Ren XF, Herrler G. Select what you need. A comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes. J Biotechnol. 2007;127:335-47.
16. Poquet I, Saint V, Seznec E, Simoes N, Bolotin A, Gruss A. HtrA is the unique surface housekeeping protease in Lactococcus lactis and is required for natural protein processing. Mol Microbiol. 2000;35:1042-51.
17. Frees D, Savijoki K, Varmanen P, Ingmer H. Clp ATPases and ClpP proteolytic complexes regulate vital biological processes in low GC, Gram-positive bacteria. Mol Microbiol. 2007;63:1285-95.
18. Morello E, Nouaille S, Cortes-Perez NG, Blugeon S, Medina LF, Azevedo V, Gratadoux JJ, Bermudez-Humaran LG, Le Loir Y, Langella P. Inactivation of the ybdD gene in Lactococcus lactis increases the amounts of exported proteins. Appl Environ Microbiol. 2012;78:7148-51.
19. Krishnappa L, Monteferrante CG, Neef J, Dreisbach A, van Dijl JM. Degradation of extracytoplasmic catalysts for protein folding in Bacillus subtilis. Appl Environ Microbiol. 2014;80:1463-8.
20. van Dijl JM, Hecker M. Bacillus subtilis: from soil bacterium to super-secreting cell factory. Microb Cell Fact. 2013;12:1.
21. Zhu DL, Zhao K, Xu HJ, Zhang XM, Bai YL, Saris PEJ, Qiao MQ. Construction of thyA deficient Lactococcus lactis using the Cre-loxP recombination system. Ann Microbiol. 2015;65:1659-65.
22. Garcia-Cayuela T, de Cadinanos LP, Mohedano ML, de Palencia PF, Boden D, Wells J, Pelaez C, Lopez P, Requena T. Fluorescent protein vectors for promoter analysis in lactic acid bacteria and Escherichia coli. Appl Microbiol Biotechnol. 2012;96:171-81.
23. Wan X, Li R, Saris PE, Takala TM. Genetic characterisation and heterologous expression of leucocin C, a class IIa bacteriocin from Leuconostoc carnosum 4010. Appl Microbiol Biotechnol. 2013;97:3509-18.
24. Zhu DL, Liu FL, Xu HJ, Bai YL, Zhang XM, Saris PEJ, Qiao MQ. Isolation of strong constitutive promoters from Lactococcus lactis subsp lactis N8. Fems Microbiol Letter. 2015;362:fnv107.
25. Ubeda C, Maiques E, Barry P, Matthews A, Tormo MA, Lasa I, Novick RP, Penades JR. SaPI mutations affecting replication and transfer and enabling autonomous replication in the absence of helper phage. Mol Microbiol. 2008;67:493-503.
26. Mahony J, Martel B, Tremblay DM, Neve H, Heller KJ, Moineau S, van Sinderen D. Identification of a new P335 subgroup through molecular analysis of lactococcal phages Q33 and BM13. Appl Environ Microbiol. 2013;79:4401-9.
27. Keravala A, Lee S, Thyagarajan B, Olivares EC, Gabrovsky VE, Woodard LE, Calos MP. Mutational derivatives of PhiC31 integrase with increased efficiency and specificity. Mol Ther. 2009;17:112-20.
28. Bahey-El-Din M, Griffin BT, Gahan CGM. Nisin inducible production of listeriolysin O in Lactococcus lactis NZ9000. Microb Cell Fact. 2008;7:1.
29. Hernandez I, Molenaar D, Beekwilder J, Bouwmeester H, Vlieg JETV. Expression of plant flavor genes in Lactococcus lactis. Appl Environ Microbiol. 2007;73:1544-52.
30. Gao G, Qiao JJ, Yang CH, Jiang DZ, Li RQ, Su JJ, Xu HJ, Zhang XM, Bai YL, Qiao MQ. Functional expression of mouse insulin-like growth factor-I with food-grade vector in Lactococcus lactis NZ9000. Lett Appl Microbiol. 2012;54:404-9.
31. Berenbaum MR, Zangerl AR. Costs of Inducible Defense: protein limitation, growth, and detoxification in Parsnip Webworms. Ecology. 1994;75:2311-7.
32. Kolisnychenko V, Plunkett G, Herring CD, Feher T, Posfai J, Blattner FR, Posfai G. Engineering a reduced Escherichia coli genome. Genome Res. 2002;12:640-7.
33. Woodcock DM, Crowther PJ, Doherty J, Jefferson S, DeCruz E, Noyer-Weidner M, Smith SS, Michael MZ, Graham MW. Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res. 1989;17:3469-78.
34. Valdesstauber N, Gotz H, Busse M. Antagonistic effect of coryneform bacteria from red smear cheese against Listeria Species. Int J Food Microbiol. 1991;13:119-30.
35. Qiao M, Immonen T, Koponen O, Saris PE. The cellular location and effect on nisin immunity of the NisI protein from Lactococcus lactis N8 expressed in Escherichia coli and L. lactis. FEMS Microbiol Lett. 1995;131:75-80.
36. Kuipers OP, de Ruyter PGGA, Kleerebezem M, de Vos WM. Quorum sensing-controlled gene expression in lactic acid bacteria. J Biotechnol. 1998;64:15-21.
37. Lambert JM, Bongers RS, Kjeerebezem M. Cre-lox-based system for multiple gene deletions and selectable-marker removal in Lactobacillus plantarum. Appl Environ Microbiol. 2007;73:1126-35.
38. Ra SR, Qiao MQ, Immonen T, Pujana I, Saris PEJ. Genes responsible for nisin synthesis, regulation and immunity form a regulon of two operons and are induced by nisin in Lactococcus lactis N8. Microbiology-Uk. 1996;142:1281-8.
39. Jensen PR, Hammer K. Minimal requirements for exponential growth of Lactococcus lactis. Appl Microbiol Biotechnol. 1993;59:4363-6.
40. Holo H, Nes IF. High-frequency transformation, by Electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media. Appl Environ Microbiol. 1989;55:3119-23.
41. Tang X, Nakata Y, Li HO, Zhang M, Gao H, Fujita A, Sakatsume O, Ohta T, Yokoyama K. The optimization of preparations of competent cells for transformation of E. coli. Nucleic Acids Res. 1994;22:2857-8.
42. Nakamura M, Mie M, Funabashi H, Yamamoto K, Ando J, Kobatake E. Cell-surface-localized ATP detection with immobilized firefly luciferase. Anal Biochem. 2006;352:61-7.
43. Kimmich GA, Randles J, Brand JS. Assay of picomole amounts of ATP, ADP, and AMP using the luciferase enzyme system. Anal Biochem. 1975;69:187-206.
44. Castro R, Neves AR, Fonseca LL, Pool WA, Kok J, Kuipers OP, Santos H. Characterization of the individual glucose uptake systems of Lactococcus lactis: mannose-PTS, cellobiose-PTS and the novel GlcU permease. Mol Microbiol. 2009;71:795-806.
45. -ΔΔCT method. Methods. 2001;25:402-8.
46. Pongtharangkul T, Demirci A. Evaluation of agar diffusion bioassay for nisin quantification. Appl Microbiol Biotechnol. 2004;65:268-72.
47. Ungvari Z, Orosz Z, Rivera A, Labinskyy N, Zhao XM, Olson S, Podlutsky A, Csiszar A. Resveratrol increases vascular oxidative stress resistance. AM J Physiol-Heart C. 2007;292:H2417-24.