Broad-range detection of microorganisms directly from bronchoalveolar lavage specimens by PCR/electrospray ionization-mass spectrometry

PLoS ONE

Volumn 12, Issue 1, 2017, Pages

  Ullberg, Måns ^a,b   Lüthje, Petra ^a,b   Mölling, Paula ^c   Strålin, Kristoffer ^a,b   Özenci, Volkan ^a,b  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

^c Örebro University   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BORDETELLA PERTUSSIS; CLINICAL STUDY; COMMENSAL; CRITICALLY ILL PATIENT; ELECTROSPRAY MASS SPECTROMETRY; HUMAN; IDENTITY; LAVAGE FLUID; LEGIONELLA PNEUMOPHILA; LUNG LAVAGE; MYCOPLASMA PNEUMONIAE; NONHUMAN; POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS PNEUMONIAE; BACTERIUM; BRONCHOALVEOLAR LAVAGE FLUID; CLASSIFICATION; GENETICS; ISOLATION AND PURIFICATION; LUNG DISEASES; MICROBIOLOGY; PROCEDURES;

BACTERIAL DNA;

BACTERIA; BRONCHOALVEOLAR LAVAGE FLUID; DNA, BACTERIAL; HUMANS; LUNG DISEASES; POLYMERASE CHAIN REACTION; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 85009280997     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0170033     Document Type: Article

References (26)

1. Welte T, Torres A, Nathwani D. Clinical and economic burden of community-acquired pneumonia among adults in Europe. Thorax. 2012; 67: 71-9. doi: 10.1136/thx.2009.129502 PMID: 20729232
2. Arnold FW, Wiemken TL, Peyrani P, Ramirez JA, Brock GN, authors C. Mortality differences among hospitalized patients with community-acquired pneumonia in three world regions: results from the Community-Acquired Pneumonia Organization (CAPO) International Cohort Study. Respir Med. 2013; 107: 1101-11. doi: 10.1016/j.rmed.2013.04.003 PMID: 23660396
3. Prina E, Ranzani OT, Torres A. Community-acquired pneumonia. Lancet. 2015; 386: 1097-108. doi: 10.1016/S0140-6736(15)60733-4 PMID: 26277247
4. Brown JS. Geography and the aetiology of community-acquired pneumonia. Respirology. 2009; 14: 1068-71. doi: 10.1111/j.1440-1843.2009.01641.x PMID: 19909456
5. Cilloniz C, Ewig S, Polverino E, Marcos MA, Prina E, Sellares J, et al. Community-acquired pneumonia in outpatients: aetiology and outcomes. Eur Respir J. 2012; 40: 931-8. doi: 10.1183/09031936.00168811 PMID: 22267760
6. Torres A, Ewig S, Lode H, Carlet J. Defining, treating and preventing hospital acquired pneumonia: European perspective. Intensive Care Med. 2009; 35: 9-29. doi: 10.1007/s00134-008-1336-9 PMID: 18989656
7. Jones RN. Microbial etiologies of hospital-acquired bacterial pneumonia and ventilator-associated bacterial pneumonia. Clin Infect Dis. 2010; 51 Suppl 1: S81-7.
8. Mandell LA, Wunderink RG, Anzueto A, Bartlett JG, Campbell GD, Dean NC, et al. Infectious Diseases Society of America/American Thoracic Society consensus guidelines on the management of community-acquired pneumonia in adults. Clin Infect Dis. 2007; 44 Suppl 2: S27-72.
9. Souweine B, Veber B, Bedos JP, Gachot B, Dombret MC, Regnier B, et al. Diagnostic accuracy of protected specimen brush and bronchoalveolar lavage in nosocomial pneumonia: impact of previous antimicrobial treatments. Crit Care Med. 1998; 26: 236-44. PMID: 9468159
10. Kottmann RM, Kelly J, Lyda E, Gurell M, Stalica J, Ormsby W, et al. Bronchoscopy with bronchoalveolar lavage: determinants of yield and impact on management in immunosuppressed patients. Thorax. 2011; 66: 823.
11. Baudel JL, Tankovic J, Dahoumane R, Carrat F, Galbois A, Ait-Oufella H, et al. Multiplex PCR performed of bronchoalveolar lavage fluid increases pathogen identification rate in critically ill patients with pneumonia: a pilot study. Ann Intensive Care. 2014; 4: 35. doi: 10.1186/s13613-014-0035-7 PMID: 25593751
12. Kunze N, Moerer O, Steinmetz N, Schulze MH, Quintel M, Perl T. Point-of-care multiplex PCR promises short turnaround times for microbial testing in hospital-acquired pneumonia - an observational pilot study in critical ill patients. Ann Clin Microbiol Antimicrob. 2015; 14: 33. doi: 10.1186/s12941-015-0091-3 PMID: 26071191
13. Gadsby NJ, McHugh MP, Russell CD, Mark H, Conway Morris A, Laurenson IF, et al. Development of two real-time multiplex PCR assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections. Clin Microbiol Infect. 2015; 21: 788 e1-e13.
14. Jordana-Lluch E, Gimenez M, Quesada MD, Ausina V, Martro E. Improving the diagnosis of bloodstream infections: PCR coupled with mass spectrometry. Biomed Res Int. 2014; 2014: 501214. doi: 10.1155/2014/501214 PMID: 24818144
15. Jeng K, Hardick J, Rothman R, Yang S, Won H, Peterson S, et al. Reverse transcription-PCR-electrospray ionization mass spectrometry for rapid detection of biothreat and common respiratory pathogens. J Clin Microbiol. 2013; 51: 3300-7. doi: 10.1128/JCM.01443-13 PMID: 23903543
16. Huttner A, Emonet S, Harbarth S, Renzi G, Kaiser L, Schrenzel J. Polymerase-chain reaction/electrospray ionization-mass spectrometry for the detection of bacteria and fungi in bronchoalveolar lavage fluids: a prospective observational study. Clin Microbiol Infect. 2014; 20: O1059-66. doi: 10.1111/1469-0691.12749 PMID: 25529027
17. Vincent JL, Brealey D, Libert N, Abidi NE, O'Dwyer M, Zacharowski K, et al. Rapid diagnosis of infection in the critically ill, a multicenter study of molecular detection in bloodstream infections, pneumonia, and sterile site infections. Crit Care Med. 2015; 43: 2283-91. doi: 10.1097/CCM.0000000000001249 PMID: 26327198
18. Hedberg ST, Olcen P, Fredlund H, Molling P. Real-time PCR detection of five prevalent bacteria causing acute meningitis. APMIS. 2009; 117: 856-60. doi: 10.1111/j.1600-0463.2009.02539.x PMID: 19845537
19. Berglund C, Molling P, Sjoberg L, Soderquist B. Predominance of staphylococcal cassette chromosome mec (SCCmec) type IV among methicillin-resistant Staphylococcus aureus (MRSA) in a Swedish county and presence of unknown SCCmec types with Panton-Valentine leukocidin genes. Clin Microbiol Infect. 2005; 11: 447-56. doi: 10.1111/j.1469-0691.2005.01150.x PMID: 15882194
20. Arbique JC, Poyart C, Trieu-Cuot P, Quesne G, Carvalho Mda G, Steigerwalt AG, et al. Accuracy of phenotypic and genotypic testing for identification of Streptococcus pneumoniae and description of Streptococcus pseudopneumoniae sp. nov. J Clin Microbiol. 2004; 42: 4686-96. doi: 10.1128/JCM.42.10.4686-4696.2004 PMID: 15472328
21. Carvalho Mda G, Tondella ML, McCaustland K, Weidlich L, McGee L, Mayer LW, et al. Evaluation and improvement of real-time PCR assays targeting lytA, ply, and psaA genes for detection of pneumococcal DNA. J Clin Microbiol. 2007; 45: 2460-6. doi: 10.1128/JCM.02498-06 PMID: 17537936
22. Nilsson P, Ripa T. Staphylococcus aureus throat colonization is more frequent than colonization in the anterior nares. J Clin Microbiol. 2006; 44: 3334-9. doi: 10.1128/JCM.00880-06 PMID: 16954269
23. Nurjadi D, Lependu J, Kremsner PG, Zanger P. Staphylococcus aureus throat carriage is associated with ABO-/secretor status. J Infect. 2012; 65: 310-7. doi: 10.1016/j.jinf.2012.05.011 PMID: 22664149
24. Johnson AP. Methicillin-resistant Staphylococcus aureus: the European landscape. J Antimicrob Chemother. 2011; 66 Suppl 4: iv43-iv8.
25. Laupland KB, Lyytikainen O, Sogaard M, Kennedy KJ, Knudsen JD, Ostergaard C, et al. The changing epidemiology of Staphylococcus aureus bloodstream infection: a multinational population-based surveillance study. Clin Microbiol Infect. 2013; 19: 465-71. doi: 10.1111/j.1469-0691.2012.03903.x PMID: 22616816
26. Strålin K, Ehn F, Giske CG, Ullberg M, Hedlund J, Petersson J, et al. The IRIDICA PCR/Electrospray Ionization-Mass Spectrometry assay on bronchoalveolar lavage for bacterial etiology in mechanically ventilated patients with suspected pneumonia. PLoS One. 2016; 11: e0159694. doi: 10.1371/journal.pone.0159694 PMID: 27463099