A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants

Molecular Plant

Volumn 9, Issue 7, 2016, Pages 1088-1091

  Tang, Xu ^a,b   Zheng, Xuelian ^a   Qi, Yiping ^c   Zhang, Dengwei ^a   Cheng, Yan ^a   Tang, Aiting ^a   Voytas, Daniel F ^b   Zhang, Yong ^a  

^a UNIVERSITY OF ELECTRONIC SCIENCE AND TECHNOLOGY OF CHINA   (China)

^b UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^c EAST CAROLINA UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PLANT PROTEIN;

CRISPR CAS SYSTEM; GENE EDITING; GENETICS; METABOLISM; PHYSIOLOGY; PLANT GENOME; PROCEDURES;

CRISPR-CAS SYSTEMS; GENE EDITING; GENOME, PLANT; PLANT PROTEINS;

EID: 85009100580     PISSN: 16742052     EISSN: 17529867     Source Type: Journal    
DOI: 10.1016/j.molp.2016.05.001     Document Type: Letter

References (11)

1. Dower, K., Kuperwasser, N., Merrikh, H., Rosbash, M., A synthetic A tail rescues yeast nuclear accumulation of a ribozyme-terminated transcript. RNA 10 (2004), 1888–1899.
2. Gao, Y., Zhao, Y., Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. J. Integr. Plant Biol. 56 (2014), 343–349.
3. Haseloff, J., Gerlach, W.L., Simple RNA enzymes with new and highly specific endoribonuclease activities. Nature 334 (1988), 585–591.
4. Lowder, L.G., Zhang, D., Baltes, N.J., Paul, J.W. 3rd, Tang, X., Zheng, X., Voytas, D.F., Hsieh, T.F., Zhang, Y., Qi, Y., A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 169 (2015), 971–985.
5. Puchta, H., Using CRISPR/Cas in three dimensions: towards synthetic plant genomes, transcriptomes and epigenomes. Plant J., 2016, 10.1111/tpj.13100.
6. Ran, F.A., Hsu, P.D., Lin, C.Y., Gootenberg, J.S., Konermann, S., Trevino, A.E., Scott, D.A., Inoue, A., Matoba, S., Zhang, Y., et al. Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell 154 (2013), 1380–1389.
7. Sun, X., Hu, Z., Chen, R., Jiang, Q., Song, G., Zhang, H., Xi, Y., Targeted mutagenesis in soybean using the CRISPR-Cas9 system. Sci. Rep., 5, 2015, 10342.
8. Tsai, S.Q., Wyvekens, N., Khayter, C., Foden, J.A., Thapar, V., Reyon, D., Goodwin, M.J., Aryee, M.J., Joung, J.K., Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat. Biotechnol. 32 (2014), 569–576.
9. Xie, K., Minkenberg, B., Yang, Y., Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc. Natl. Acad. Sci. USA 112 (2015), 3570–3575.
10. Yoshioka, S., Fujii, W., Ogawa, T., Sugiura, K., Naito, K., Development of a mono-promoter-driven CRISPR/Cas9 system in mammalian cells. Sci. Rep., 5, 2015, 18341.
11. Zuo, J., Niu, Q.W., Chua, N.H., Technical advance: an estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants. Plant J. 24 (2000), 265–273.