Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker

Plant Biotechnology Journal

Volumn 15, Issue 7, 2017, Pages 817-823

  Jia, Hongge ^a   Zhang, Yunzeng ^a   Orbović, Vladimir ^a   Xu, Jin ^a   White, Frank F ^a   Jones, Jeffrey B ^a   Wang, Nian ^a  

^a UNIVERSITY OF FLORIDA   (United States)

Author keywords

Cas9; Citrus paradisi; sgRNA; Xanthomonas citri

Indexed keywords

PLANT RNA;

CITRUS; DISEASE PREDISPOSITION; DISEASE RESISTANCE; GENE EDITING; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MICROBIOLOGY; PLANT DISEASE; TRANSGENIC PLANT;

CITRUS; DISEASE RESISTANCE; DISEASE SUSCEPTIBILITY; GENE EDITING; GENE EXPRESSION REGULATION, PLANT; PLANT DISEASES; PLANTS, GENETICALLY MODIFIED; RNA, PLANT;

EID: 85007557300     PISSN: 14677644     EISSN: 14677652     Source Type: Journal    
DOI: 10.1111/pbi.12677     Document Type: Article

References (22)

1. Blanvillain-Baufumé, S., Reschke, M., Solé, M., Auguy, F., Doucoure, H., Szurek, B., Meynard, D. et al. (2016) Targeted promoter editing for rice resistance to Xanthomonas oryzae pv. oryzae reveals differential activities for SWEET14-inducing TAL effectors. Plant Biotechnol. doi:10.1111/pbi.12613.
2. Chen, X., Barnaby, J., Sreedharan, A., Huang, X., Orbovic, V., Grosser, J., Wang, N. et al. (2013) Over-expression of the citrus gene CtNH1 confers resistance to bacterial canker disease. Physiol. Mol. Plant Pathol. 84, 115–122.
3. Davey, M.R., Anthony, P., Power, J.B. and Lowe, K.C. (2005) Plant protoplasts: status and biotechnological perspectives. Biotechnol. Adv. 23, 131–171.
4. Doudna, J.A. and Charpentier, E. (2014) Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science, 346, 1258096.
5. Dutt, M., Barthe, G., Irey, M. and Grosser, J. (2015) Transgenic citrus expressing an Arabidopsis NPR1 gene exhibit enhanced resistance against Huanglongbing (HLB; Citrus Greening). PLoS ONE, 10, e0137134.
6. Edgar, R.C. (2010) Search and clustering orders of magnitude faster than BLAST. Bioinformatics, 26, 2460–2461.
7. Fass, J.N., Joshi, N.A., Couvillion, M.T., Bowen, J., Gorovsky, M.A., Hamilton, E.P., Orias, E. et al. (2011) Genome-scale analysis of programmed DNA elimination sites in Tetrahymena thermophila. G3: Genes – Genomes – Genet. 1, 515–522.
8. Fu, X.Z., Chen, C.W., Wang, Y., Liu, J.H. and Moriguchi, T. (2011) Ectopic expression of MdSPDS1 in sweet orange (Citrus sinensis Osbeck) reduces canker susceptibility: involvement of H₂O₂ production and transcriptional alteration. BMC Plant Biol. 11, 55.
9. Hu, Y., Zhang, J., Jia, H., Sosso, D., Li, T., Frommer, W.B., Yang, B. et al. (2014) Lateral organ boundaries 1 is a disease susceptibility gene for citrus bacterial canker disease. Proc. Natl. Acad. Sci. USA, 111, E521–E529.
10. Husbands, A., Bell, E.M., Shuai, B., Smith, H.M. and Springer, P.S. (2007) LATERAL ORGAN BOUNDARIES defines a new family of DNA-binding transcription factors and can interact with specific bHLH proteins. Nucleic Acids Res. 35, 6663–6671.
11. Jia, H. and Wang, N. (2014) Xcc-facilitated agroinfiltration of citrus leaves: a tool for rapid functional analysis of transgenes in citrus leaves. Plant Cell Rep. 33, 1993–2001.
12. Jia, H., Orbovic, V., Jones, J.B. and Wang, N. (2016) Modification of the PthA4 effector binding elements in Type I CsLOB1 promoter using Cas9/sgRNA to produce transgenic Duncan grapefruit alleviating XccΔpthA4:dCsLOB1.3 infection. Plant Biotechnol. J. 14, 1291–1301.
13. Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J.A. and Charpentier, E. (2012) A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science, 337, 816–821.
14. Li, T., Liu, B., Spalding, M.H., Weeks, D.P. and Yang, B. (2012) High-efficiency TALEN-based gene editing produces disease-resistant rice. Nat. Biotechnol. 30, 390–392.
15. Orbović, V. and Grosser, J.W. (2015) Citrus transformation using juvenile tissue explants. Methods Mol. Biol. 1224, 245–257.
16. Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725–2729.
17. Velasco, R. and Licciardello, C. (2014) A genealogy of the citrus family. Nat. Biotechnol. 32, 640–642.
18. Waltz, E. (2016a) CRISPR-edited crops free to enter market, skip regulation. Nat. Biotechnol. 34, 582.
19. Waltz, E. (2016b) Gene-edited CRISPR mushroom escapes US regulation. Nature, 532, 293.
20. Yordanov, Y.S., Regan, S. and Busov, V. (2010) Members of the LATERAL ORGAN BOUNDARIES DOMAIN transcription factor family are involved in the regulation of secondary growth in Populus. Plant Cell, 22, 3662–3677.
21. Zhang, J., Carlos Huguet Tapia, J., Hu, Y., Jones, J., Wang, N., Liu, S. and White, F.F. (2016) Homologs of CsLOB1 in citrus function as disease susceptibility genes in citrus canker. Mol. Plant Pathol. doi: 10.1111/mpp.12441.
22. Zhou, J., Peng, Z., Long, J., Sosso, D., Liu, B., Eom, J.S., Huang, S. et al. (2015) Gene targeting by the TAL effector PthXo2 reveals cryptic resistance gene for bacterial blight of rice. Plant J. 82, 632–643.