Functional analysis of histone deacetylase 11 (HDAC11)

Methods in Molecular Biology

Volumn 1436, Issue , 2016, Pages 147-165

  Chen, Jie ^a   Sahakian, Eva ^a   Powers, John ^a   Lienlaf, Maritza ^a   Perez Villarroel, Patricio ^a   Knox, Tessa ^a   Villagra, Alejandro ^a  

^a H LEE MOFFITT CANCER CENTER AND RESEARCH INSTITUTE   (United States)

Author keywords

Chromatin immunoprecipitation; Flow cytometry; Genotyping; Histone deacetylase 11; PCR; Phenotyping; QRT PCR

Indexed keywords

COMPLEMENTARY DNA; GENOMIC DNA; HISTONE DEACETYLASE 11; INTERLEUKIN 10; CYTOKINE; HDAC11 PROTEIN, MOUSE; HISTONE DEACETYLASE;

ANIMAL CELL; BONE MARROW CELL; CELL CULTURE; CELL DIFFERENTIATION; CHROMATIN IMMUNOPRECIPITATION; CONTROLLED STUDY; CROSS LINKING; DNA PURIFICATION; ENZYME ANALYSIS; FLOW CYTOMETRY; GENE INACTIVATION; GENOTYPE; GENOTYPING TECHNIQUE; IMMUNOLOGICAL PARAMETERS; MACROPHAGE; MOUSE; MYELOPOIESIS; NEUTROPHIL; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FUNCTION; REAL TIME POLYMERASE CHAIN REACTION; REPORTER GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ANIMAL; ANTIGEN PRESENTING CELL; CYTOLOGY; GENE EXPRESSION REGULATION; GENETICS; IMMUNOLOGY; METABOLISM; MYELOID-DERIVED SUPPRESSOR CELL; PROCEDURES;

ANIMALS; ANTIGEN-PRESENTING CELLS; CHROMATIN IMMUNOPRECIPITATION; CYTOKINES; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; HISTONE DEACETYLASES; MICE; MYELOID-DERIVED SUPPRESSOR CELLS; PROMOTER REGIONS, GENETIC;

EID: 85007507216     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-3667-0_11     Document Type: Chapter

References (18)

1. Villagra A, Sotomayor EM, Seto E (2010) Histone deacetylases and the immunological network: implications in cancer and inflammation. Oncogene 29(2):157–173
2. Foster SL, Hargreaves DC, Medzhitov R (2007) Gene-specific control of inflammation by TLR-induced chromatin modifications. Nature 447(7147):972–978
3. Woan KV, Sahakian E, Sotomayor EM, Seto E, Villagra A (2012) Modulation of antigen-presenting cells by HDAC inhibitors: implications in autoimmunity and cancer. Immunol Cell Biol 90(1):55–65
4. Miremadi A, Oestergaard MZ, Pharoah PDP, Caldas C (2007) Cancer genetics of epigenetic genes. Hum Mol Genet 16(R1):R28–R49
5. Schreiber RD, Old LJ, Smyth MJ (2011) Cancer immunoediting: integrating immunity’s roles in cancer suppression and promotion. Science 331(6024):1565–1570
6. Gao L, Cueto MA, Asselbergs F, Atadja P (2002) Cloning and functional characterization of HDAC11, a novel member of the human histone deacetylase family. J Biol Chem 277(28):25748–25755
7. Sahakian E, Powers JJ, Chen J et al (2014) Histone deacetylase 11: a novel epigenetic regulator of myeloid derived suppressor cell expansion and function. Mol Immunol 63(2):579–585
8. Cheng F, Lienlaf M, Perez-Villarroel P et al (2014) Divergent roles of histone deacetylase 6 (HDAC6) and histone deacetylase 11 (HDAC11) on the transcriptional regulation of IL10 in antigen presenting cells. Mol Immunol 60(1):44–53
9. Gong S, Zheng C, Doughty ML et al (2003) A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature 425(6961):917–925
10. Heintz N (2001) BAC to the future: the use of bac transgenic mice for neuroscience research. Nat Rev Neurosci 2(12):861–870
11. Sahakian E, Powers JJ, Chen J et al (2015) Histone deacetylase 11: a novel epigenetic regulator of myeloid derived suppressor cell expansion and function. Mol Immunol 63(2):579–585
12. Wang H, Cheng F, Woan K et al (2011) Histone deacetylase inhibitor LAQ824 augments inflammatory responses in macrophages through transcriptional regulation of IL-10. J Immunol 186(7):3986–3996
13. Villagra A, Cheng F, Wang HW et al (2009) The histone deacetylase HDAC11 regulates the expression of interleukin 10 and immune tolerance. Nat Immunol 10(1):92–100
14. Cheng F, Lienlaf M, Wang HW et al (2014) A novel role for histone deacetylase 6 in the regulation of the tolerogenic STAT3/IL-10 pathway in APCs. J Immunol 193(6): 2850–2862
15. Cheng F, Wang H, Horna P et al (2012) Stat3 inhibition augments the immunogenicity of B-cell lymphoma cells, leading to effective antitumor immunity. Cancer Res 72(17): 4440–4448
16. Eva Sahakian JC, John J. Powers, Xianghong Chen, Kamira Maharaj, Susan L. Deng, Maritza Lienlaf, Hong Wei Wang, Andressa L. Sodré, Allison Distler, Limin Xing, Patricio Perez-Villarroel, Sheng Wei, Alejandro Villagra, Ed Seto, Eduardo M. Sotomayor, Pedro Horna and Javier Pinilla-Ibarz (2015) Essential regulatory role for histone deacetylase 11 (HDAC11) In neutrophil function. J Leukoc Biol. Under 2nd revision
17. Lee TI, Johnstone SE, Young RA (2006) Chromatin immunoprecipitation and microarray-based analysis of protein location. Nat Protoc 1(2):729–748
18. Nelson JD, Denisenko O, Bomsztyk K (2006) Protocol for the fast chromatin immunoprecipitation (ChIP) method. Nat Protoc 1(1): 179–185