Assays for the screening and characterization of tau aggregation inhibitors

Methods in Molecular Biology

Volumn 1523, Issue , 2017, Pages 129-140

  Rickard, Janet E ^a,b   Horsley, David ^a,b   Wischik, Claude M ^a,b   Harrington, Charles R ^a,b  

^a UNIVERSITY OF ABERDEEN   (United Kingdom)

^b TAURX THERAPEUTICS LTD   (United Kingdom)

Author keywords

Alzheimer s disease; ELISA; Immunoassay; Tau aggregation inhibitor; Tau protein

Indexed keywords

PROTEIN INHIBITOR; TAU PROTEIN; NEUROPROTECTIVE AGENT;

ALZHEIMER DISEASE; ANIMAL CELL; ANIMAL TISSUE; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; FIBROBLAST; IC50; IMMUNOASSAY; IMMUNOBLOTTING; LD50; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; SURFACE PROPERTY; TAUOPATHY; THERAPEUTIC INDEX; ANIMAL; ANTAGONISTS AND INHIBITORS; BIOASSAY; CELL LINE; METABOLISM; MOUSE; PROCEDURES; PROTEINOSIS;

ALZHEIMER DISEASE; ANIMALS; BIOLOGICAL ASSAY; CELL LINE; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOASSAY; MICE; NEUROPROTECTIVE AGENTS; PROTEIN AGGREGATION, PATHOLOGICAL; TAU PROTEINS;

EID: 85006483718     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-6598-4_8     Document Type: Chapter

References (18)

1. Buée L, Bussière T, Buée-Scherrer V et al (2000) Tau protein isoforms, phosphorylation and role in neurodegenerative disorders. Brain Res Rev 33:95–130
2. Novak M, Kabat J, Wischik CM (1993) Molecular characterization of the minimal protease resistant tau unit of the Alzheimer’s disease paired helical filament. EMBO J 12:365–370
3. Wischik CM, Novak M, Thøgersen HC et al (1988) Isolation of a fragment of tau derived from the core of the paired helical filament of Alzheimer’s disease. Proc Natl Acad Sci U S A 85:4506–4510
4. Mukaetova-Ladinska EB, Garcia-Sierra F, Hurt J et al (2000) Staging of cytoskeletal and b-amyloid changes in human isocortex reveals biphasic synaptic protein response during progression of Alzheimer’s disease. Am J Pathol 157:623–636
5. Arriagada PW, Growdon JH, Hedley-White ET, Hyman BT (1992) Neurofibrillary tangles but not senile plaques parallel duration and severity of Alzheimer’s disease. Neurology 42:631–639
6. Wilcock GK, Esiri MM (1982) Plaques, tangles and dementia: a quantitative study. J Neurol Sci 56:343–356
7. Maruyama M, Shimada H, Suhara T et al (2013) Imaging of tau pathology in a tauopathy mouse model and in Alzheimer patients compared to normal controls. Neuron 79:1094–1108
8. Okamura N, Furumoto S, Fodero-Tavoletti MT et al (2014) Non-invasive assessment of Alzheimer’s disease neurofibrillary pathology using F-18-THK5105 PET. Brain 137:1762–1771
9. Wischik CM, Edwards PC, Lai RYK et al (1996) Selective inhibition of Alzheimer disease-like tau aggregation by phenothiazines. Proc Natl Acad Sci U S A 93:11213–11218
10. Wischik CM, Staff RT, Wischik DJ et al (2015) Tau aggregation inhibitor therapy: an exploratory phase 2 study in mild or moderate Alzheimer’s disease. J Alzheimers Dis 44:705–720
11. Bulic B, Pickhardt M, Mandelkow E (2013) Progress and developments in tau aggregation inhibitors for Alzheimer disease. J Med Chem 56:4135–4155
12. Taniguchi S, Suzuki N, Masuda M et al (2005) Inhibition of heparin-induced tau filament formation by phenothiazines, polyphenols, and porphyrins. J Biol Chem 280:7614–7623
13. Harrington CR, Storey JMD, Clunas S et al (2015) Cellular models of aggregation-dependent template-directed proteolysis to characterize tau aggregation inhibitors for treatment of Alzheimer’s disease. J Biol Chem 290:10862–10875
14. Melis V, Magbagbeolu M, Rickard JE et al (2015) Effects of oxidized and reduced forms of methylthioninium in two transgenic mouse tauopathy models. Behav Pharmacol 26:353–368
15. Brandt R, Kempf M, Lee G (1997) Expression and purification of tau for in vitro studies. In: Avila J, Brandt R, Kosik KS (eds) Brain microtubule associated proteins: modifications in disease. Harwood Academic Publishers, Amsterdam, pp 245–257
16. Matsudaira PT, Burgess DR (1978) SDS microslab linear gradient polyacrylamide gel electrophoresis. Anal Biochem 87:386–396
17. Novak M, Jakes R, Edwards PC et al (1991) Difference between the tau protein of Alzheimer paired helical filament core and normal tau revealed by epitope analysis of mAbs 423 and 7.51. Proc Natl Acad Sci U S A 88:5837–5841
18. Harrington CR, Edwards PC, Wischik CM (1990) Competitive ELISA for measurement of tau proteins in Alzheimer’s disease. J Immunol Methods 134:261–271