Enhancement of the direct antimicrobial activity of Lysep3 against Escherichia coli by inserting cationic peptides into its C terminus

Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

Volumn 110, Issue 3, 2017, Pages 347-355

  Ma, Qiang ^a   Guo, Zhimin ^a,b   Gao, Chencheng ^a   Zhu, Rining ^a   Wang, Shuang ^a   Yu, Ling ^a   Qin, Wanhai ^a   Xia, Xiaojing ^a   Gu, Jingmin ^a   Yan, Guangmou ^a   Lei, Liancheng ^a  

^a JILIN UNIVERSITY   (China)

^b Basic Medical College   (China)

Author keywords

Antimicrobial resistance; Bacteriophage; Bacteriophage lysins; Escherichia coli; Gram negative bacteria; Superbugs

Indexed keywords

AMINO ACID DERIVATIVE; ANTIINFECTIVE AGENT; CATION; HYBRID PROTEIN; LYSEP3 PROTEIN; LYSIN DERIVATIVE; LYSYLARGINYLLYSYLARGINYLLYSINE; LYSYLARGINYLLYSYLARGINYLLYSYLARGINYLLYSYLARGINYLLYSYLARGININE; LYSYLARGINYLLYSYLARGINYLLYSYLARGINYLLYSYLARGINYLLYSYLARGINYLLYSYLARGINYLLYSYLARGINYLLYSINE; LYSYLARGINYLLYSYLARGINYLLYSYLPHENYLALANYLPHENYLALANYLVALYLALANYLISOLEUCYLISOLEUCYLPROLINE; PEPTIDE DERIVATIVE; PEPTIDOGLYCAN; POLYPEPTIDE; UNCLASSIFIED DRUG; ANTIMICROBIAL CATIONIC PEPTIDE; VIRAL PROTEIN; VIRUS DNA;

ANTIMICROBIAL ACTIVITY; ARTICLE; BACTERIAL CELL WALL; BACTERIAL OUTER MEMBRANE; BACTERICIDAL ACTIVITY; BACTERIOPHAGE; BIOENGINEERING; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DRUG STRUCTURE; ESCHERICHIA COLI; HYDROPHOBICITY; IN VITRO STUDY; NONHUMAN; PROTEIN HYDROLYSIS; AMINO ACID SEQUENCE; BACTERIOLYSIS; CELL WALL; COLIPHAGE; DRUG EFFECTS; DRUG POTENTIATION; GENETIC ENGINEERING; GENETICS; GRAM-POSITIVE BACTERIAL INFECTIONS; METABOLISM; PROCEDURES; VIROLOGY;

AMINO ACID SEQUENCE; ANTIMICROBIAL CATIONIC PEPTIDES; BACTERIOLYSIS; CELL WALL; COLIPHAGES; DNA, VIRAL; DRUG SYNERGISM; ESCHERICHIA COLI; GENETIC ENGINEERING; GRAM-POSITIVE BACTERIAL INFECTIONS; PEPTIDOGLYCAN; VIRAL PROTEINS;

EID: 85004045015     PISSN: 00036072     EISSN: 15729699     Source Type: Journal    
DOI: 10.1007/s10482-016-0806-2     Document Type: Article

References (28)

1. Arima H, Ibrahim HR, Kinoshita T, Kato A (1997) Bactericidal action of lysozymes attached with various sizes of hydrophobic peptides to the C-terminal using genetic modification. FEBS Lett 415:114–118
2. Briers Y, Lavigne R (2015) Breaking barriers: expansion of the use of endolysins as novel antibacterials against Gram-negative bacteria. Future Microbiol 10:377–390
3. Briers Y, Volckaert G, Cornelissen A, Lagaert S, Michiels CW, Hertveldt K, Lavigne R (2007) Muralytic activity and modular structure of the endolysins of Pseudomonas aeruginosa bacteriophages phiKZ and EL. Mol Microbiol 65:1334–1344
4. Briers Y, Schmelcher M, Loessner MJ, Hendrix J, Engelborghs Y, Volckaert G, Lavigne R (2009) The high-affinity peptidoglycan binding domain of Pseudomonas phage endolysin KZ144. Biochem Biophys Res Commun 383:187–191
5. Briers Y, Walmagh M, Lavigne R (2011) Use of bacteriophage endolysin EL188 and outer membrane permeabilizers against Pseudomonas aeruginosa. J Appl Microbiol 110:778–785
6. Briers Y, Walmagh M, Lavigne R (2014) Engineered endolysin-based “Artilysins” to combat multidrug-resistant Gram-negative pathogens. MBio 5:e1314–e1379
7. Gayoso CM, Mateos J, Mendez JA, Fernandez-Puente P, Rumbo C, Tomas M, Martinez DIO, Bou G (2014) Molecular mechanisms involved in the response to desiccation stress and persistence in Acinetobacter baumannii. J Proteome Res 13:460–476
8. Gerstmans H, Rodriguez-Rubio L, Lavigne R, Briers Y (2016) From endolysins to Artilysin(R)s: novel enzyme-based approaches to kill drug-resistant bacteria. Biochem Soc Trans 44:123–128
9. Hawkins C, Harper D, Burch D, Anggard E, Soothill J (2010) Topical treatment of Pseudomonas aeruginosa otitis of dogs with a bacteriophage mixture: a before/after clinical trial. Vet Microbiol 146:309–313
10. Ibrahim HR, Yamada M, Matsushita K, Kobayashi K, Kato A (1994) Enhanced bactericidal action of lysozyme to Escherichia coli by inserting a hydrophobic pentapeptide into its C terminus. J Biol Chem 269:5059–5063
11. Laxminarayan R, Duse A, Wattal C, Zaidi AK, Wertheim HF, Sumpradit N, Vlieghe E, Hara GL, Gould IM, Goossens H, Greko C, So AD, Bigdeli M, Tomson G, Woodhouse W, Ombaka E, Peralta AQ, Qamar FN, Mir F, Kariuki S, Bhutta ZA, Coates A, Bergstrom R, Wright GD, Brown ED, Cars O (2013) Antibiotic resistance-the need for global solutions. Lancet Infect Dis 13:1057–1098
12. Li XZ, Plesiat P, Nikaido H (2015) The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria. Clin Microbiol Rev 28:337–418
13. Li M, Li M, Lin H, Wang J, Jin Y, Han F (2016) Characterization of the novel T4-like Salmonella enterica bacteriophage STP4-a and its endolysin. Arch Virol 161:377–384
14. Loeffler JM, Nelson D, Fischetti VA (2001) Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolase. Science 294:2170–2172
15. Lood R, Winer BY, Pelzek AJ, Diez-Martinez R, Thandar M, Euler CW, Schuch R, Fischetti VA (2015) Novel phage lysin capable of killing the multidrug-resistant gram-negative bacterium Acinetobacter baumannii in a mouse bacteremia model. Antimicrob Agents Chemother 59:1983–1991
16. Lukacik P, Barnard TJ, Keller PW, Chaturvedi KS, Seddiki N, Fairman JW, Noinaj N, Kirby TL, Henderson JP, Steven AC, Hinnebusch BJ, Buchanan SK (2012) Structural engineering of a phage lysin that targets Gram-negative pathogens. Proc Natl Acad Sci USA 109:9857–9862
17. Lv M, Wang S, Yan G, Sun C, Feng X, Gu J, Han W, Lei L (2015) Genome sequencing and analysis of an Escherichia coli phage vB_EcoM-ep3 with a novel lysin, Lysep3. Virus Genes 50:487–497
18. Maal-Bared R, Bartlett KH, Bowie WR, Hall ER (2013) Phenotypic antibiotic resistance of Escherichia coli and E. coli O157 isolated from water, sediment and biofilms in an agricultural watershed in British Columbia. Sci Total Environ 443:315–323
19. Morita M, Tanji Y, Orito Y, Mizoguchi K, Soejima A, Unno H (2001) Functional analysis of antibacterial activity of Bacillus amyloliquefaciens phage endolysin against Gram-negative bacteria. FEBS Lett 500:56–59
20. Obeso JM, Martinez B, Rodriguez A, Garcia P (2008) Lytic activity of the recombinant staphylococcal bacteriophage PhiH5 endolysin active against Staphylococcus aureus in milk. Int J Food Microbiol 128:212–218
21. Orito Y, Morita M, Hori K, Unno H, Tanji Y (2004) Bacillus amyloliquefaciens phage endolysin can enhance permeability of Pseudomonas aeruginosa outer membrane and induce cell lysis. Appl Microbiol Biotechnol 65:105–109
22. Pastagia M, Schuch R, Fischetti VA, Huang DB (2013) Lysins: the arrival of pathogen-directed anti-infectives. J Med Microbiol 62:1506–1516
23. Potron A, Poirel L, Nordmann P (2015) Emerging broad-spectrum resistance in Pseudomonas aeruginosa and Acinetobacter baumannii: mechanisms and epidemiology. Int J Antimicrob Agents 45:568–585
24. Sass P, Bierbaum G (2007) Lytic activity of recombinant bacteriophage phi11 and phi12 endolysins on whole cells and biofilms of Staphylococcus aureus. Appl Environ Microbiol 73:347–352
25. Schmelcher M, Loessner MJ (2016) Bacteriophage endolysins: applications for food safety. Curr Opin Biotechnol 37:76–87
26. Schmelcher M, Donovan DM, Loessner MJ (2012) Bacteriophage endolysins as novel antimicrobials. Future Microbiol 7:1147–1171
27. Thandar M, Lood R, Winer BY, Deutsch DR, Euler CW, Fischetti VA (2016) Novel engineered peptides of a phage lysin as effective antimicrobials against multidrug-resistant Acinetobacter baumannii. Antimicrob Agents Chemother 60:2671–2679
28. Walmagh M, Boczkowska B, Grymonprez B, Briers Y, Drulis-Kawa Z, Lavigne R (2013) Characterization of five novel endolysins from Gram-negative infecting bacteriophages. Appl Microbiol Biotechnol 97:4369–4375