Quantitative and qualitative effects of bioaugmentation on ammonia oxidisers at a two-step WWTP

Water Science and Technology

Volumn 61, Issue 4, 2010, Pages 1003-1009

  Podmirseg, S M ^a   Schoen, M A ^a   Murthy, S ^b   Insam, H ^a   Wett, B ^a  

^a UNIVERSITY OF INNSBRUCK   (Austria)

^b District of Columbia Water and Sewer Authority   (United States)

Author keywords

Ammonia oxidising bacteria; Bioaugmentation; DGGE; Habitat change; Melt curve analysis; qRT PCR

Indexed keywords

AMMONIA; BACTERIOLOGY; ECOLOGY; ELECTROPHORESIS; NITRIFICATION; SEWAGE; SEWAGE TREATMENT PLANTS; SLUDGE DISPOSAL; WASTEWATER; WASTEWATER TREATMENT;

AMMONIA OXIDISING BACTERIA; BIO-AUGMENTATION; CURVE ANALYSIS; DENATURING GRADIENT GEL ELECTROPHORESIS; DIFFERENT PROCESS; HABITAT CHANGE; HIGH STRENGTH; NITRIFICATION/DENITRIFICATION; PUMP RATE; Q-RT-PCR; REAL-TIME PCR; SLUDGE DEWATERING; TWO STAGE; WASTE ACTIVATED SLUDGES; WASTEWATER TREATMENT PLANTS;

ACTIVATED SLUDGE PROCESS;

AMMONIA; SERINE PROTEINASE OMI;

AMMONIA; DEWATERING; HABITAT SELECTION; MICROBIAL ACTIVITY; NITRIFICATION; OXIDATION; POLYMERASE CHAIN REACTION; SEWAGE TREATMENT; WASTEWATER;

ACTIVATED SLUDGE; BIOMASS; CONFERENCE PAPER; DENITRIFICATION; GEL ELECTROPHORESIS; NITRIFICATION; NITROSOMONAS EUROPAEA; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEWAGE; SLUDGE DEWATERING; SLUDGE DIGESTION; SPECIES DIFFERENCE; WASTE WATER TREATMENT PLANT;

EID: 85000259339     PISSN: 02731223     EISSN: None     Source Type: Book Series    
DOI: 10.2166/wst.2010.016     Document Type: Article

References (18)

1. Aakra, A., Utåker, J. B. & Nes, I. F. 1999 RFLP of rRNA genes and sequencing of the 16S-23S rDNA intergenic spacer region of ammonia-oxidizing bacteria: a phylogenetic approach. Int. J. Syst. Bacteriol. 49(Pt 1), 123-130.
2. Amann, R. I., Ludwig, W. & Schleifer, K. H. 1995 Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59(1), 143-169.
3. Boon, N., Top, E., Verstraete, W.& Siciliano, S. 2003 Bioaugmentation as a tool to protect the structure and function of an activated-sludge microbial community against a 3-chloroaniline shock load. Appl. Environ.Microbiol. 69(3), 1511-1520.
4. Hermansson, A. & Lindgren, P. E. 2001 Quantification of ammonia-oxidizing bacteria in arable soil by real-time PCR. Appl. Environ. Microbiol. 67(2), 972-976.
5. Heuer, H., Krsek, M., Baker, P., Smalla, K. & Wellington, E. M. 1997 Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients. Appl. Environ. Microbiol. 63(8), 3233-3241.
6. Hooper, A. B., Vannelli, T., Bergmann, D. J. & Arciero, D. M. 1997 Enzymology of the oxidation of ammonia to nitrite by bacteria. Antonie Van Leeuwenhoek 71(1-2), 59-67.
7. Kowalchuk, G. A., Stephen, J. R., De Boer, W., Prosser, J. I., Embley, T. M. &Woldendorp, J. W. 1997 Analysis of ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteria in coastal sand dunes by denaturing gradient gel electrophoresis and sequencing of PCR-amplified 16S ribosomal DNA fragments. Appl. Environ. Microbiol. 63(4), 1489-1497.
8. Krhutková, O., Novák, L., Pachmanová, L., Benáková, A., Wanner, J. & Kos, M. 2006 In situ bioaugmentation of nitrification in the regeneration zone: practical application and experiences at full-scale plants. Water Sci. Technol. 53(12), 39.
9. Marchesi, J. R., Sato, T., Weightman, A. J., Martin, T. A., Fry, J. C., Hiom, S. J., Dymock, D. & Wade, W. G. 1998 Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl. Environ. Microbiol. 64(6), 2333.
10. Mertoglu, B., Semerci, N., Guler, N., Calli, B., Cecen, F. & Saatci, A. M. 2008 Monitoring of population shifts in an enriched nitrifying system under gradually increased cadmium loading. J. Hazard. Mater. 160(2-3), 495-501.
11. Nicolaisen, M. H. & Ramsing, N. B. 2002 Denaturing gradient gel electrophoresis (DGGE) approaches to study the diversity of ammonia-oxidizing bacteria. J. Microbiol. Methods 50, 189-203.
12. Rittmann, B. E. 1996 How input active biomass affects sludge age and process stability. J. Environ. Eng. 122(1), 4-8.
13. Salem, S., Berends, D. H. J. G., Heijnen, J. J. & Van Loosdrecht, M. C. M. 2003 Bio-augmentation by nitrification with return sludge. Water Res. 37(8), 1794-1804.
14. Sanguinetti, C. J., Dias Neto, E. & Simpson, A. J. 1994 Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. BioTechniques 17(5), 914-921.
15. Schoen, M., Podmirseg, S. M., Murthy, S. & Wett, B. Nitrifier bioaugmentation-development of a biomass transfer scheme between sidestream process and 2-step mainstream treatment for optimised N-removal. (In preparation)
16. Smith, R. C., Saikaly, P. E., Zhang, K., Thomatos, S. & Oerther, D. B. 2008 Ecological engineering of bioaugmentation from side-stream nitrification. Water Sci. Technol. 57(12), 1927-1933.
17. Wagner, M., Rath, G., Amann, R., Koops, H. P. & Schleifer, K. H. 1995 In situ identification of ammonia-oxidizing bacteria. Syst. Appl. Microbiol. 18, 251-264.
18. Zhang, T. & Fang, H. H. P. 2006 Applications of real-time polymerase chain reaction for quantification of microorganisms in environmental samples. Appl. Microbiol. Biotechnol. 70, 281-289.