Evaluation of PacBio sequencing for full-length bacterial 16S rRNA gene classification

BMC Microbiology

Volumn 16, Issue 1, 2016, Pages 1-17

  Wagner, Josef ^a   Coupland, Paul ^a   Browne, Hilary P ^a   Lawley, Trevor D ^a   Francis, Suzanna C ^b   Parkhill, Julian ^a  

^a WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^b LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

Author keywords

Capillary sequencing; Full length bacterial 16S rRNA gene sequencing; MiSeq sequencing; PacBio sequencing

Indexed keywords

ANALYTICAL ERROR; ARTICLE; BACTERIAL GENE; BACTERIUM COLONY; BACTERIUM IDENTIFICATION; BIOINFORMATICS; COMPUTER MODEL; CONTROLLED STUDY; FECES MICROFLORA; GENE SEQUENCE; GENETIC VARIABILITY; MICROBIAL DIVERSITY; NONHUMAN; RNA 16S GENE; RNA GENE; RNA SEQUENCE; SEQUENCE ANALYSIS; STAPHYLOCOCCUS AUREUS; VAGINA FLORA; BIODIVERSITY; BIOLOGY; CLASSIFICATION; DEVICES; DNA SEQUENCE; FEMALE; GENETIC VARIATION; GENETICS; HIGH THROUGHPUT SEQUENCING; HUMAN; METAGENOME; MICROBIOLOGY; MICROFLORA; NUCLEOTIDE SEQUENCE; PHYLOGENY; PROCEDURES; VAGINA;

BACTERIAL DNA; RIBOSOME DNA; RNA 16S;

BASE SEQUENCE; BIODIVERSITY; COMPUTATIONAL BIOLOGY; DNA, BACTERIAL; DNA, RIBOSOMAL; FEMALE; GENETIC VARIATION; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; METAGENOME; MICROBIOTA; PHYLOGENY; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; STAPHYLOCOCCUS AUREUS; VAGINA;

EID: 84994751887     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/s12866-016-0891-4     Document Type: Article

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