Assessment of lymphocyte proliferation for diagnostic purpose: Comparison of CFSE staining, Ki-67 expression and 3H-thymidine incorporation

Human Immunology

Volumn 77, Issue 12, 2016, Pages 1215-1222

  Lašťovička, Jan ^a   Rataj, Michal ^a   Bartůňková, Jiřina ^a  

^a UNIVERSITY HOSPITAL MOTOL   (Czech Republic)

Author keywords

3H thymidine incorporation; CFSE staining; Flow cytometry; Ki 67 expression; Lymphocyte proliferation; Phytohemagglutinin

Indexed keywords

CARBOXYFLUORESCEIN; KI 67 ANTIGEN; THYMIDINE; 5-(6)-CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; FLUORESCEIN DERIVATIVE; SUCCINIMIDE DERIVATIVE;

ANTIGEN EXPRESSION; ARTICLE; CONTROLLED STUDY; CORRELATION COEFFICIENT; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOCOMPROMISED PATIENT; LYMPHOCYTE PROLIFERATION; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; THYMIDINE INCORPORATION ASSAY; CELL CULTURE; CELL PROLIFERATION; COHORT ANALYSIS; COMPARATIVE STUDY; IMMUNOASSAY; IMMUNOLOGICAL MONITORING; IMMUNOLOGY; LYMPHOCYTE; LYMPHOCYTE ACTIVATION; METABOLISM; PROCEDURES; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY;

CELL PROLIFERATION; CELLS, CULTURED; COHORT STUDIES; FLOW CYTOMETRY; FLUORESCEINS; HUMANS; IMMUNOASSAY; IMMUNOCOMPROMISED HOST; KI-67 ANTIGEN; LYMPHOCYTE ACTIVATION; LYMPHOCYTES; MONITORING, IMMUNOLOGIC; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SUCCINIMIDES; THYMIDINE;

EID: 84994584949     PISSN: 01988859     EISSN: 18791166     Source Type: Journal    
DOI: 10.1016/j.humimm.2016.08.012     Document Type: Article

References (37)

1. [1] Rosato, M.T., et al. Simultaneous analysis of surface marker expression and cell cycle progression in human peripheral blood mononuclear cells. J. Immunol. Methods 256:1–2 (2001), 35–46.
2. [2] Motobu, M., et al. Detection of mitogen-induced lymphocyte proliferation by bromodeoxyuridine (BrdU) incorporation in the chicken. J. Vet. Med. Sci. 64:4 (2002), 377–379.
3. [3] Svahn, A., et al. Development and evaluation of a flow-cytometric assay of specific cell-mediated immune response in activated whole blood for the detection of cell-mediated immunity against varicella-zoster virus. J. Immunol. Methods 277:1–2 (2003), 17–25.
4. [4] Marits, P., et al. Evaluation of T and B lymphocyte function in clinical practice using a flow cytometry based proliferation assay. Clin. Immunol. 153:2 (2014), 332–342.
5. [5] Dengler, W.A., et al. Development of a propidium iodide fluorescence assay for proliferation and cytotoxicity assays. Anticancer Drugs 6:4 (1995), 522–532.
6. [6] Suzuki, T., et al. DNA staining for fluorescence and laser confocal microscopy. J. Histochem. Cytochem. 45:1 (1997), 49–53.
7. [7] Ching, R.H., et al. Phosphorylation of Nucleophosmin at Threonine 234/237 is associated with HCC metastasis. Oncotarget, 2015.
8. [8] Leonardi, E., et al. PCNA and Ki67 expression in breast carcinoma: correlations with clinical and biological variables. J. Clin. Pathol. 45:5 (1992), 416–419.
9. [9] Wilson, G.D., Flow cytometric detection of proliferation-associated antigens, PCNA and Ki-67. Methods Mol. Biol. 80 (1998), 355–363.
10. [10] Last'ovicka, J., et al. Assessment of lymphocyte proliferation: CFSE kills dividing cells and modulates expression of activation markers. Cell Immunol. 256:1–2 (2009), 79–85.
11. [11] Zolnierowicz, J., et al. Monitoring cell proliferation in vitro with different cellular fluorescent dyes. Folia Histochem. Cytobiol. 51:3 (2013), 193–200.
12. [12] Soares, A., et al. Novel application of Ki67 to quantify antigen-specific in vitro lymphoproliferation. J. Immunol. Methods 362:1–2 (2010), 43–50.
13. [13] Muskhelishvili, L., et al. Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67(MIB-5) immunohistochemistry and in situ hybridization for histone mRNA. J. Histochem. Cytochem. 51:12 (2003), 1681–1688.
14. [14] Palutke, M., KuKuruga, D., Tabaczka, P., A flow cytometric method for measuring lymphocyte proliferation directly from tissue culture plates using Ki-67 and propidium iodide. J. Immunol. Methods 105:1 (1987), 97–105.
15. [15] Misawa, Y., Desoxyribonucleic acid synthesizing peripheral blood cells studied by in vitro uptake of 3-H-thymidine. 1. The age distribution of desoxyribonucleic acid synthesizing blood cells in normal persons. Yokohama Med. Bull. 11 (1960), 397–406.
16. [16] Morimoto, K., Wolff, S., Cell cycle kinetics in human lymphocyte cultures. Nature 288:5791 (1980), 604–606.
17. [17] Pollack, A., et al. The kinetics of the formation of a G2 block from tritiated thymidine in phytohemagglutinin-stimulated human lymphocytes. Cytometry 1:1 (1980), 57–64.
18. [18] Hu, V.W., et al. 3H-thymidine is a defective tool with which to measure rates of DNA synthesis. FASEB J. 16:11 (2002), 1456–1457.
19. [19] Cleaver, J.E., Thomas, G.H., Burki, H.J., Biological damage from intranuclear tritium: DNA strand breaks and their repair. Science 177:4053 (1972), 996–998.
20. [20] Yanokura, M., et al. Cell death and cell-cycle arrest induced by incorporation of [3H]thymidine into human haemopoietic cell lines. Int. J. Radiat. Biol. 76:3 (2000), 295–303.
21. [21] Pollack, A., Bagwell, C.B., Irvin, G.L. 3rd, Radiation from tritiated thymidine perturbs the cell cycle progression of stimulated lymphocytes. Science 203:4384 (1979), 1025–1027.
22. [22] Maurer, H.R., Potential pitfalls of [3H]thymidine techniques to measure cell proliferation. Cell Tissue Kinet. 14:2 (1981), 111–120.
23. [23] Wang, B., et al. Induction of apoptosis by beta radiation from tritium compounds in mouse embryonic brain cells. Health Phys. 77:1 (1999), 16–23.
24. [24] Beltrami, A.P., et al. Evidence that human cardiac myocytes divide after myocardial infarction. N. Engl. J. Med. 344:23 (2001), 1750–1757.
25. [25] Gerdes, J., et al. Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J. Immunol. 133:4 (1984), 1710–1715.
26. [26] Endl, E., et al. Analysis of cell cycle-related Ki-67 and p120 expression by flow cytometric BrdUrd-Hoechst/7AAD and immunolabeling technique. Cytometry 29:3 (1997), 233–241.
27. [27] Perreau, M., et al. Follicular helper T cells serve as the major CD4 T cell compartment for HIV-1 infection, replication, and production. J. Exp. Med. 210:1 (2013), 143–156.
28. [28] Muellenbeck, M.F., et al. Atypical and classical memory B cells produce Plasmodium falciparum neutralizing antibodies. J. Exp. Med. 210:2 (2013), 389–399.
29. [29] Jones, J.L., et al. Human autoimmunity after lymphocyte depletion is caused by homeostatic T-cell proliferation. Proc. Natl. Acad. Sci. U.S.A 110:50 (2013), 20200–20205.
30. [30] Stubbe, M., et al. Antigen-specific central memory CD4+ T lymphocytes produce multiple cytokines and proliferate in vivo in humans. J. Immunol. 177:11 (2006), 8185–8190.
31. [31] Miller, J.D., et al. Human effector and memory CD8+ T cell responses to smallpox and yellow fever vaccines. Immunity 28:5 (2008), 710–722.
32. [32] Barth, J., et al. Proliferation of alveolar macrophages in pulmonary sarcoidosis correlates with markers of lymphocyte activity in bronchoalveolar lavage fluid. Pneumologie 44:Suppl 1 (1990), 224–225.
33. [33] Zambon, A.C., Use of the Ki67 promoter to label cell cycle entry in living cells. Cytometry A 77:6 (2010), 564–570.
34. [34] Fulcher, D., Wong, S., Carboxyfluorescein succinimidyl ester-based proliferative assays for assessment of T cell function in the diagnostic laboratory. Immunol. Cell Biol. 77:6 (1999), 559–564.
35. [35] Wang, X.Q., et al. Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling. Acta Biochim. Biophys. Sin (Shanghai) 37:6 (2005), 379–385.
36. [36] Garza, E.V., Skovbo, A., Dige, A.K., Hokland, M., Development of a flow cytometry assay combining cell proliferation, viability and two specific surface markers. Medicina Universitaria 13:53 (2011), 183–192.
37. [37] Lis, H., Sharon, N., Erythrina lectins. Methods Enzymol. 138 (1987), 544–551.