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Volumn 126, Issue , 2016, Pages 29-37

Influence of pomegranate seed oil and bitter melon aqueous extract on polyunsaturated fatty acids and their lipoxygenase metabolites concentration in serum of rats

Author keywords

Bitter melon; Conjugated linolenic acid; HEPE; HETE; HODE; Pomegranate seed oil

Indexed keywords

12 HYDROXYICOSATETRAENOIC ACID; 15 HYDROXYICOSATETRAENOIC ACID; 5 HYDROXYICOSATETRAENOIC ACID; ARACHIDONIC ACID; BITTER MELON AQUEOUS EXTRACT; DOCOSAHEXAENOIC ACID; GAMMA LINOLENIC ACID; HYDROXYICOSATETRAENOIC ACID; HYDROXYOCTADECADIENOIC ACID; ICOSAPENTAENOIC ACID; LINOLEIC ACID; LINOLENIC ACID; LIPOXYGENASE; MOMORDICA CHARANTIA EXTRACT; MONOUNSATURATED FATTY ACID; OLEIC ACID; OMEGA 3 FATTY ACID; OMEGA 6 FATTY ACID; PALMITIC ACID; POLYUNSATURATED FATTY ACID; STEARIC ACID; UNCLASSIFIED DRUG; VEGETABLE OIL; PLANT EXTRACT; UNSATURATED FATTY ACID; WATER;

EID: 84992379129     PISSN: 10988823     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.prostaglandins.2016.07.005     Document Type: Article
Times cited : (14)

References (42)
  • 1
    • 84878655508 scopus 로고    scopus 로고
    • Conjugated linoleic acid as a potential protective factor in prevention of breast cancer
    • abstract in English
    • [1] Białek, A., Tokarz, A., Conjugated linoleic acid as a potential protective factor in prevention of breast cancer. Postepy Hig. Med. Dosw. 67 (2013), 6–14 abstract in English.
    • (2013) Postepy Hig. Med. Dosw. , vol.67 , pp. 6-14
    • Białek, A.1    Tokarz, A.2
  • 2
    • 84903317174 scopus 로고    scopus 로고
    • Conjugated linolenic acids and their bioactivities: a review
    • [2] Yuan, G.F., Chen, X.E., Li, D., Conjugated linolenic acids and their bioactivities: a review. Food Funct. 5 (2014), 1360–1368.
    • (2014) Food Funct. , vol.5 , pp. 1360-1368
    • Yuan, G.F.1    Chen, X.E.2    Li, D.3
  • 3
    • 33947402295 scopus 로고    scopus 로고
    • Determination of conjugated linolenic acid content of selected oil seeds grown in Turkey
    • [3] Ozgul-Yucel, S., Determination of conjugated linolenic acid content of selected oil seeds grown in Turkey. JAOCS 82 (2005), 893–897.
    • (2005) JAOCS , vol.82 , pp. 893-897
    • Ozgul-Yucel, S.1
  • 4
    • 0031762531 scopus 로고    scopus 로고
    • Nutritional characteristics of oil containing conjugated octadecatrienoic fatty acid
    • [4] Dhar, P., Bhattacharyya, D.K., Nutritional characteristics of oil containing conjugated octadecatrienoic fatty acid. Ann. Nutr. Metab. 42 (1998), 290–296.
    • (1998) Ann. Nutr. Metab. , vol.42 , pp. 290-296
    • Dhar, P.1    Bhattacharyya, D.K.2
  • 5
    • 27644555543 scopus 로고    scopus 로고
    • Conjugated fatty acids in food and their health benefits
    • [5] Nagao, K., Yanagita, T., Conjugated fatty acids in food and their health benefits. J. Biosci. Bioeng. 100 (2005), 152–157.
    • (2005) J. Biosci. Bioeng. , vol.100 , pp. 152-157
    • Nagao, K.1    Yanagita, T.2
  • 6
    • 84860620064 scopus 로고    scopus 로고
    • Obesity: the preventive role of the pomegranate
    • [6] Al-Muammar, M.N., Khan, F., Obesity: the preventive role of the pomegranate. Nutrition 28 (2012), 595–604.
    • (2012) Nutrition , vol.28 , pp. 595-604
    • Al-Muammar, M.N.1    Khan, F.2
  • 7
    • 12344279393 scopus 로고    scopus 로고
    • Dietary effect of pomegranate seed oil rich in 9cis, 11trans, 13cis conjugated linolenic acid on lipid metabolism in obese, hyperlipidemic OLETF rats
    • [7] Arao, K., Wang, Y.M., Inoue, N., Hirata, J., Cha, J.Y., Nagao, K., Yanagita, T., Dietary effect of pomegranate seed oil rich in 9cis, 11trans, 13cis conjugated linolenic acid on lipid metabolism in obese, hyperlipidemic OLETF rats. Lipids Health Dis., 3, 2004, 24.
    • (2004) Lipids Health Dis. , vol.3 , pp. 24
    • Arao, K.1    Wang, Y.M.2    Inoue, N.3    Hirata, J.4    Cha, J.Y.5    Nagao, K.6    Yanagita, T.7
  • 8
    • 33646150462 scopus 로고    scopus 로고
    • Absorbtion and metabolism of conjugated α-linolenic acid given as free fatty acids or triacylglycerols in rats
    • [8] Plourde, M., Sergiel, J.P., Chardigny, J.M., Gregoire, S., Angers, P., Sebedio, J.P., Absorbtion and metabolism of conjugated α-linolenic acid given as free fatty acids or triacylglycerols in rats. Nutr. Metab., 3, 2006, 8.
    • (2006) Nutr. Metab. , vol.3 , pp. 8
    • Plourde, M.1    Sergiel, J.P.2    Chardigny, J.M.3    Gregoire, S.4    Angers, P.5    Sebedio, J.P.6
  • 9
    • 33644608183 scopus 로고    scopus 로고
    • Dietary comparison of conjugated linolenic acid (9 cis, 11 trans, 13 trans) and α-tocopherol effects on blood lipids and lipid peroxidation on alloxan-induced diabetes mellitus in rats
    • [9] Dhar, P., Bhattacharyya, D., Bhattacharyya, D.K., Ghosh, S., Dietary comparison of conjugated linolenic acid (9 cis, 11 trans, 13 trans) and α-tocopherol effects on blood lipids and lipid peroxidation on alloxan-induced diabetes mellitus in rats. Lipids 41 (2006), 49–54.
    • (2006) Lipids , vol.41 , pp. 49-54
    • Dhar, P.1    Bhattacharyya, D.2    Bhattacharyya, D.K.3    Ghosh, S.4
  • 11
    • 84911906309 scopus 로고    scopus 로고
    • Influence of maternal diet enrichment with conjugated linoleic acids on lipoxygenase metabolites of polyunsaturated fatty acids in serum of their offspring with 7,12-dimethylbenz[a]anthracene induced mammary tumors
    • [11] Białek, A., Jelińska, M., Tokarz, A., Influence of maternal diet enrichment with conjugated linoleic acids on lipoxygenase metabolites of polyunsaturated fatty acids in serum of their offspring with 7,12-dimethylbenz[a]anthracene induced mammary tumors. Prostaglandins Other Lipid Mediators 116–117 (2015), 10–18.
    • (2015) Prostaglandins Other Lipid Mediators , vol.116-117 , pp. 10-18
    • Białek, A.1    Jelińska, M.2    Tokarz, A.3
  • 12
    • 84907967256 scopus 로고    scopus 로고
    • Effect of conjugated linoleic acid mixture supplemented daily after carcinogen application on linoleic and arachidonic acid metabolites in rat serum and induced tumours
    • [12] Jelińska, M., Białek, A., Mojska, H., Gielecińska, I., Tokarz, A., Effect of conjugated linoleic acid mixture supplemented daily after carcinogen application on linoleic and arachidonic acid metabolites in rat serum and induced tumours. Biochim. Biophys. Acta 1842 (2014), 2230–2236 http://dx.doi.org/10.1016/j.bbadis.2014.08.013.
    • (2014) Biochim. Biophys. Acta , vol.1842 , pp. 2230-2236
    • Jelińska, M.1    Białek, A.2    Mojska, H.3    Gielecińska, I.4    Tokarz, A.5
  • 13
    • 47549111139 scopus 로고    scopus 로고
    • Therapeutic applications of Pomegranate (Punica granatum L.): a review
    • [13] Jurenka, J., Therapeutic applications of Pomegranate (Punica granatum L.): a review. Altern. Med. Rev. 13 (2008), 128–144.
    • (2008) Altern. Med. Rev. , vol.13 , pp. 128-144
    • Jurenka, J.1
  • 14
    • 84920579412 scopus 로고    scopus 로고
    • Granat—bogate źródło antyutleniaczy
    • abstract in English
    • [14] Rumpel, L., Granat—bogate źródło antyutleniaczy. Panacea 38 (2012), 16–17 abstract in English.
    • (2012) Panacea , vol.38 , pp. 16-17
    • Rumpel, L.1
  • 15
    • 77950347612 scopus 로고    scopus 로고
    • Pomegranate and breast cancer: possible mechanisms of prevention
    • [15] Sturgeon, S.R., Ronnenberg, A.G., Pomegranate and breast cancer: possible mechanisms of prevention. Nutr. Rev. 68 (2009), 122–128.
    • (2009) Nutr. Rev. , vol.68 , pp. 122-128
    • Sturgeon, S.R.1    Ronnenberg, A.G.2
  • 16
    • 33746386763 scopus 로고    scopus 로고
    • Bitter gourd (Momordica charantia): a dietary approach to hyperglycemia
    • [16] Krawinkel, M.B., Keding, G.B., Bitter gourd (Momordica charantia): a dietary approach to hyperglycemia. Nutr. Rev. 64 (2006), 331–337.
    • (2006) Nutr. Rev. , vol.64 , pp. 331-337
    • Krawinkel, M.B.1    Keding, G.B.2
  • 18
    • 33746603115 scopus 로고    scopus 로고
    • Conjugated linolenic acid is slowly absorbed in rat intestine, but quickly converted to conjugated linoleic acid
    • [18] Tsuzuki, T., Kawakami, Y., Abe, R., Nakagawa, K., Koba, K., Imamura, J., Iwata, T., Ikeda, I., Miyazawa, T., Conjugated linolenic acid is slowly absorbed in rat intestine, but quickly converted to conjugated linoleic acid. J. Nutr. 136 (2006), 2153–2159.
    • (2006) J. Nutr. , vol.136 , pp. 2153-2159
    • Tsuzuki, T.1    Kawakami, Y.2    Abe, R.3    Nakagawa, K.4    Koba, K.5    Imamura, J.6    Iwata, T.7    Ikeda, I.8    Miyazawa, T.9
  • 19
    • 2942538292 scopus 로고    scopus 로고
    • Pharmacological actions and potential uses of Momordica charantia: a review
    • [19] Grover, J.K., Yaday, S.P., Pharmacological actions and potential uses of Momordica charantia: a review. J. Ethnopharmacol. 93 (2004), 123–132.
    • (2004) J. Ethnopharmacol. , vol.93 , pp. 123-132
    • Grover, J.K.1    Yaday, S.P.2
  • 20
    • 4744370985 scopus 로고    scopus 로고
    • Alpha-eleostearic acid (9Z11E13E-18:3) is quickly converted to conjugated linoleic acid (9Z11E-18:2) in rats
    • [20] Tsuzuki, T., Tokuyama, Y., Igarashi, M., Nakagawa, K., Ohsaki, Y., Komai, M., Alpha-eleostearic acid (9Z11E13E-18:3) is quickly converted to conjugated linoleic acid (9Z11E-18:2) in rats. J. Nutr. 134 (2004), 2634–2639.
    • (2004) J. Nutr. , vol.134 , pp. 2634-2639
    • Tsuzuki, T.1    Tokuyama, Y.2    Igarashi, M.3    Nakagawa, K.4    Ohsaki, Y.5    Komai, M.6
  • 22
    • 33646854376 scopus 로고    scopus 로고
    • Monitoring eicosanoid biosynthesis via lipoxygenase and cyclooxygenase pathways in human whole blood by single HPLC run
    • [22] Frohberg, P., Drutkowski, G., Wobst, J., Monitoring eicosanoid biosynthesis via lipoxygenase and cyclooxygenase pathways in human whole blood by single HPLC run. J. Pharm. Biomed. Anal. 41 (2006), 1317–1324.
    • (2006) J. Pharm. Biomed. Anal. , vol.41 , pp. 1317-1324
    • Frohberg, P.1    Drutkowski, G.2    Wobst, J.3
  • 23
    • 70349810455 scopus 로고    scopus 로고
    • Incorporation and metabolism of punicic acid in healthy young humans
    • [23] Yuan, G., Sinclair, A.J., Xu, C., Li, D., Incorporation and metabolism of punicic acid in healthy young humans. Mol. Nutr. Food Res. 53 (2009), 1336–1342.
    • (2009) Mol. Nutr. Food Res. , vol.53 , pp. 1336-1342
    • Yuan, G.1    Sinclair, A.J.2    Xu, C.3    Li, D.4
  • 24
    • 69849106842 scopus 로고    scopus 로고
    • Effects of conjugated linolenic acid and conjugated linoleic acid on lipid metabolism in mice
    • [24] Yuan, G., Sun, H., Sinclair, A.J., Li, D., Effects of conjugated linolenic acid and conjugated linoleic acid on lipid metabolism in mice. Eur. J. Lipid Sci. Technol. 111 (2009), 537–545.
    • (2009) Eur. J. Lipid Sci. Technol. , vol.111 , pp. 537-545
    • Yuan, G.1    Sun, H.2    Sinclair, A.J.3    Li, D.4
  • 26
    • 69249221592 scopus 로고    scopus 로고
    • Pomegranate seed oil consumption during a period of high-fat feeding reduces weight gain and reduces type 2 diabetes risk in CD-1 mice
    • [26] McFarlin, B.K., Strohacker, K.A., Kueht, M.L., Pomegranate seed oil consumption during a period of high-fat feeding reduces weight gain and reduces type 2 diabetes risk in CD-1 mice. Br. J. Nutr. 102 (2009), 54–59.
    • (2009) Br. J. Nutr. , vol.102 , pp. 54-59
    • McFarlin, B.K.1    Strohacker, K.A.2    Kueht, M.L.3
  • 27
    • 0034284704 scopus 로고    scopus 로고
    • Effects of Momordica charantia powder on serum glucose levels and various lipid parameters in rats fed with cholesterol-free and cholesterol-enriched diets
    • [27] Jayasooriya, A.P., Sakono, M., Yukizaki, C., Kawano, M., Yamamoto, K., Fukuda, N., Effects of Momordica charantia powder on serum glucose levels and various lipid parameters in rats fed with cholesterol-free and cholesterol-enriched diets. J. Ethnopharmacol. 72 (2000), 331–336.
    • (2000) J. Ethnopharmacol. , vol.72 , pp. 331-336
    • Jayasooriya, A.P.1    Sakono, M.2    Yukizaki, C.3    Kawano, M.4    Yamamoto, K.5    Fukuda, N.6
  • 28
    • 84864676062 scopus 로고    scopus 로고
    • Comparative study of hypocholesterolemic and hypolipidemic effects of conjugated linolenic acid isomers against induced biochemical perturbations and aberration in erythrocyte membrane fluidity
    • [28] Saha, S.S., Chakraborty, A., Ghosh, S., Ghosh, M., Comparative study of hypocholesterolemic and hypolipidemic effects of conjugated linolenic acid isomers against induced biochemical perturbations and aberration in erythrocyte membrane fluidity. Eur. J. Nutr. 51 (2012), 483–545.
    • (2012) Eur. J. Nutr. , vol.51 , pp. 483-545
    • Saha, S.S.1    Chakraborty, A.2    Ghosh, S.3    Ghosh, M.4
  • 29
    • 84905042142 scopus 로고    scopus 로고
    • Conjugated linoleic acids in diet of female rats inhibit the breats cancer formation in their offspring
    • [29] Białek, A., Tokarz, A., Zagrodzki, P., Conjugated linoleic acids in diet of female rats inhibit the breats cancer formation in their offspring. J. Food Nutr. Res. 53 (2014), 39–50.
    • (2014) J. Food Nutr. Res. , vol.53 , pp. 39-50
    • Białek, A.1    Tokarz, A.2    Zagrodzki, P.3
  • 30
    • 84920531143 scopus 로고    scopus 로고
    • The effect of conjugated linoleic acids (CLA) supplementation on the activity of enzymes participating in the formation of arachidonic acid in liver microsomes of rats-probable mechanism of CLA anticancer activity
    • [30] Stawarska, A., Białek, A., Stanimirova, I., Stawarski, T., Tokarz, A., The effect of conjugated linoleic acids (CLA) supplementation on the activity of enzymes participating in the formation of arachidonic acid in liver microsomes of rats-probable mechanism of CLA anticancer activity. Nutr. Cancer 67 (2015), 145–155.
    • (2015) Nutr. Cancer , vol.67 , pp. 145-155
    • Stawarska, A.1    Białek, A.2    Stanimirova, I.3    Stawarski, T.4    Tokarz, A.5
  • 31
    • 84907276149 scopus 로고    scopus 로고
    • Enrichment of maternal diet with conjugated linoleic acids influences desaturases activity and fatty acids profile in livers and hepatic microsomes of the offspring with 7,12-dimethylbenz[a]anthracene-induced mammary tumors
    • [31] Białek, A., Stawarska, A., Tokarz, A., Czuba, K., Konarska, A., Mazurkiewicz, M., Enrichment of maternal diet with conjugated linoleic acids influences desaturases activity and fatty acids profile in livers and hepatic microsomes of the offspring with 7,12-dimethylbenz[a]anthracene-induced mammary tumors. Acta Pol. Pharm. 71 (2014), 747–761.
    • (2014) Acta Pol. Pharm. , vol.71 , pp. 747-761
    • Białek, A.1    Stawarska, A.2    Tokarz, A.3    Czuba, K.4    Konarska, A.5    Mazurkiewicz, M.6
  • 33
    • 0033278382 scopus 로고    scopus 로고
    • 12(S)-HETE in cancer metastasis
    • [33] Tang, K., Honn, K.V., 12(S)-HETE in cancer metastasis. Adv. Exp. Med. Biol. 447 (1999), 181–191.
    • (1999) Adv. Exp. Med. Biol. , vol.447 , pp. 181-191
    • Tang, K.1    Honn, K.V.2
  • 34
    • 0035458615 scopus 로고    scopus 로고
    • Five-lipoxygenase inhibitors can mediate apoptosis in human breast cancer cell lines through complex eicosanoid interactions
    • [34] Avis, I., Hong, S.H., Martinez, A., Moody, T., Choi, Y.H., Trepel, J., Das, R., Jett, M., Mulshine, J.L., Five-lipoxygenase inhibitors can mediate apoptosis in human breast cancer cell lines through complex eicosanoid interactions. FASEB J. 15 (2001), 2007–2009.
    • (2001) FASEB J. , vol.15 , pp. 2007-2009
    • Avis, I.1    Hong, S.H.2    Martinez, A.3    Moody, T.4    Choi, Y.H.5    Trepel, J.6    Das, R.7    Jett, M.8    Mulshine, J.L.9
  • 35
    • 0028906908 scopus 로고
    • Influence of diets containing eicosapentaenoic or docosahexaenoic acid on growth and metastasis of breast cancer cells in nude mice
    • [35] Rose, D.P., Connolly, J.M., Rayburn, J., Coleman, M., Influence of diets containing eicosapentaenoic or docosahexaenoic acid on growth and metastasis of breast cancer cells in nude mice. J. Natl. Cancer Inst. 87 (1995), 587–592.
    • (1995) J. Natl. Cancer Inst. , vol.87 , pp. 587-592
    • Rose, D.P.1    Connolly, J.M.2    Rayburn, J.3    Coleman, M.4
  • 36
    • 26444469529 scopus 로고    scopus 로고
    • Attenuation of breast tumor cell growth by conjugated linoleic acid via inhibition of 5-lipoxygenase activating protein
    • [36] Kim, J.H., Hubbard, N.E., Ziboh, V., Erickson, K.L., Attenuation of breast tumor cell growth by conjugated linoleic acid via inhibition of 5-lipoxygenase activating protein. Biochim. Biophys. Acta 1736 (2005), 244–250.
    • (2005) Biochim. Biophys. Acta , vol.1736 , pp. 244-250
    • Kim, J.H.1    Hubbard, N.E.2    Ziboh, V.3    Erickson, K.L.4
  • 37
    • 13644253744 scopus 로고    scopus 로고
    • Conjugated linoleic acid reduction of murine mammary tumor cell growth through 5-hydroxyeicosatetraenoic acid
    • [37] Kim, J.H., Hubbard, N.E., Ziboh, V., Erickson, K.L., Conjugated linoleic acid reduction of murine mammary tumor cell growth through 5-hydroxyeicosatetraenoic acid. Biochim. Biophys. Acta 21 (2005), 103–109.
    • (2005) Biochim. Biophys. Acta , vol.21 , pp. 103-109
    • Kim, J.H.1    Hubbard, N.E.2    Ziboh, V.3    Erickson, K.L.4
  • 38
    • 77957608027 scopus 로고    scopus 로고
    • Effect of dietary (-3 and (-9 fatty acid rich oils on lipoxygenases and cyclooxygenases enzymes and on the growth of mammary adenocarcinoma model
    • [38] Comba, A., Meastri, D.M., Berra, M.A., Gracia, C.P., Das, U.N., Eynard, A.R., Pasqualini, M.E., Effect of dietary (-3 and (-9 fatty acid rich oils on lipoxygenases and cyclooxygenases enzymes and on the growth of mammary adenocarcinoma model. Lipids Health Dis., 9, 2010, 112 http://www.lipidworld.com/content/9/1/112.
    • (2010) Lipids Health Dis. , vol.9 , pp. 112
    • Comba, A.1    Meastri, D.M.2    Berra, M.A.3    Gracia, C.P.4    Das, U.N.5    Eynard, A.R.6    Pasqualini, M.E.7
  • 39
    • 16644369027 scopus 로고    scopus 로고
    • Effect of conjugated linoleic acid on Delta-5 desaturase activity in yeast transformed with fungal Delta-5 desaturase gene
    • [39] Chuang, L.T., Thurmond, J.M., Liu, J.W., Mukerji, P., Bray, T.M., Huang, Y.S., Effect of conjugated linoleic acid on Delta-5 desaturase activity in yeast transformed with fungal Delta-5 desaturase gene. Mol. Cell. Biochem. 265 (2004), 11–18.
    • (2004) Mol. Cell. Biochem. , vol.265 , pp. 11-18
    • Chuang, L.T.1    Thurmond, J.M.2    Liu, J.W.3    Mukerji, P.4    Bray, T.M.5    Huang, Y.S.6
  • 40
    • 17244365699 scopus 로고    scopus 로고
    • Effects of specific CLA isomers on plasma fatty acid profile and expression of desaturases in humans
    • [40] Thijssen, M.A., Malpuech-Brugère, C., Gregoire, S., Chardigny, J.M., Sébédio, J.L., Mensink, R.P., Effects of specific CLA isomers on plasma fatty acid profile and expression of desaturases in humans. Lipids 40:2 (2005), 137–145.
    • (2005) Lipids , vol.40 , Issue.2 , pp. 137-145
    • Thijssen, M.A.1    Malpuech-Brugère, C.2    Gregoire, S.3    Chardigny, J.M.4    Sébédio, J.L.5    Mensink, R.P.6
  • 41
    • 0021042804 scopus 로고
    • Stimulation of eicosapentaenoic acid metabolism in washed human platelets by 12-hydroperoxyeicosatetraenoic acid
    • [41] Morita, I., Takahashi, R., Saito, Y., Murota, S., Stimulation of eicosapentaenoic acid metabolism in washed human platelets by 12-hydroperoxyeicosatetraenoic acid. J. Biol. Chem. 258 (1983), 10197–10199.
    • (1983) J. Biol. Chem. , vol.258 , pp. 10197-10199
    • Morita, I.1    Takahashi, R.2    Saito, Y.3    Murota, S.4
  • 42
    • 84994333701 scopus 로고    scopus 로고
    • Effect of conjugated linoleic acids, free and esterified linoleic acids, and trans-vaccenic acid on rat pulmonary 15-lipoxygenase-1 activity
    • [42] Cho, H., Shoeman, D.W., Csallany, A.S., Effect of conjugated linoleic acids, free and esterified linoleic acids, and trans-vaccenic acid on rat pulmonary 15-lipoxygenase-1 activity. J. Food Sci. Nutr., 1, 2015, 004.
    • (2015) J. Food Sci. Nutr. , vol.1 , pp. 004
    • Cho, H.1    Shoeman, D.W.2    Csallany, A.S.3


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