Revised Estimates for the Number of Human and Bacteria Cells in the Body

PLoS Biology

Volumn 14, Issue 8, 2016, Pages

  Sender, Ron ^a   Fuchs, Shai ^a,b   Milo, Ron ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^b HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL CELL; HUMAN; HUMAN CELL; ADULT; ANTIBODY SPECIFICITY; BACTERIAL LOAD; BACTERIUM; CELL COUNT; CYTOLOGY; GROWTH, DEVELOPMENT AND AGING; MALE; MICROFLORA; MORPHOMETRY;

ADULT; BACTERIA; BACTERIAL LOAD; BODY WEIGHTS AND MEASURES; CELL COUNT; HUMANS; MALE; MICROBIOTA; ORGAN SPECIFICITY;

EID: 84988864262     PISSN: 15449173     EISSN: 15457885     Source Type: Journal    
DOI: 10.1371/journal.pbio.1002533     Document Type: Article

References (49)

1. Bianconi E, Piovesan A, Facchin F, Beraudi A, Casadei R, Frabetti F, et al. An estimation of the number of cells in the human body. Ann Hum Biol2013;40:463–71. doi: 10.3109/03014460.2013.80787823829164
2. Savage D, Microbial ecology of the gastrointestinal tract. Annu Rev Microbiol1977;31:107–33. 334036
3. Luckey T, Introduction to intestinal microecology. Am J Clin Nutr1972;25:1292–4. 4639749
4. Sender R, Fuchs S, Milo R, Are We Really Vastly Outnumbered? Revisiting the Ratio of Bacterial to Host Cells in Humans. Cell2016;164:337–40. doi: 10.1016/j.cell.2016.01.01326824647
5. Rosner JL, Ten Time More Microbial Cells than Body Cells in Humans?Microbe2014;9:47.
6. Snyder WS, Cook MJ, Nasset ES, Karhausen LR, Parry Howells G, Tipton IH, Report of the Task Group on Reference Man. vol. 23. Pergamon Press: Oxford; 1975. doi: 10.1016/S0074-2740(75)80015-8
7. Qin J, Li R, Raes J, Arumugam M, Burgdorf KS, Manichanh C, et al. A human gut microbial gene catalogue established by metagenomic sequencing. Nature2010;464:59–67. doi: 10.1038/nature0882120203603
8. Yatsunenko T, Rey FE, Manary MJ, Trehan I, Dominguez-Bello MG, Contreras M, et al. Human gut microbiome viewed across age and geography. Nature2012;486:222–7. doi: 10.1038/nature1105322699611
9. Berg RD, The indigenous gastrointestinal microflora. Trends Microbiol1996;4:430–5. 8950812
10. Tannock GW, Normal Microflora. 1st ed.Chapman & Hall; 1995.
11. Leyden J, McGinley K, Nordstrom K, Webster G, Skin microflora. J Invest Dermatol1987:65–72.
12. Wang Y, Moss J, Thisted R, Predictors of Body Surface Area. J Clin Anesth1992;4:4–10. 1540367
13. Eve IS, A Review of the Physiology of the Gastro-Intestinal Tract in Relation to Radiation Doses from Radioactive Materials. Health Phys1966;12:131–61. 5331053
14. Schiller C, Fröhlich C-P, Giessmann T, Siegmund W, Mönnikes H, Hosten N, et al. Intestinal fluid volumes and transit of dosage forms as assessed by magnetic resonance imaging. Aliment Pharmacol Ther2005;22:971–9. doi: 10.1111/j.1365-2036.2005.02683.x16268972
15. Pritchard SE, Marciani L, Garsed KC, Hoad CL, Thongborisute W, Roberts E, et al. Fasting and postprandial volumes of the undisturbed colon: normal values and changes in diarrhea-predominant irritable bowel syndrome measured using serial MRI. Neurogastroenterol Motil2014;26:124–30. doi: 10.1111/nmo.1224324131490
16. Sandberg TH, Nilsson M, Poulsen JL, Gram M, Frøkjær JB, Østergaard LR, et al. A novel semi-automatic segmentation method for volumetric assessment of the colon based on magnetic resonance imaging. Abdom Imaging2015;40:2232–41. doi: 10.1007/s00261-015-0475-z26054979
17. Nilsson M, Sandberg TH, Poulsen JL, Gram M, Frøkjær JB, Østergaard LR, et al. Quantification and variability in colonic volume with a novel magnetic resonance imaging method. Neurogastroenterol Motil2015;27:1755–63. doi: 10.1111/nmo.1267326598050
18. Cummings JH, Bingham SA, Eastwood MA, Heaton KW, Fecal Weight, Colon Cancer Risk, and Dietary Intake of Nonstarch Polysaccharides (Dietary Fiber). Gastroenterology1992;103:1783–9. 1333426
19. Southwell BR, Clarke MCC, Sutcliffe J, Hutson JM, Colonic transit studies: normal values for adults and children with comparison of radiological and scintigraphic methods. Pediatr Surg Int2009;25:559–72. doi: 10.1007/s00383-009-2387-x19488763
20. Van Houte J, Gibbons RJ, Studies of the cultivable flora of normal human feces. Antonie van Leeuwenhoek J Microbiol1966;32:212–22. 5296851
21. Stephen A, Cummings J, The microbial contribution to human faecal mass. J Med Microbiol1980;13:45–56. 7359576
22. Thiel R, Blaut M, An improved method for the automated enumeration of fluorescently labelled bacteria in human faeces. J Microbiol Methods2005;61:369–79. doi: 10.1016/j.mimet.2004.12.01415767013
23. Franks AH, Harmsen HJM, Gerwin C, Jansen GJ, Schut F, Gjalt W, Variations of Bacterial Populations in Human Feces Measured by Fluorescent In Situ Hybridization with Group-Specific 16S rRNA-Targeted Oligonucleotide Probes. Appl Environ Microbiol1998;64:3336–45. 9726880
24. Bratbak G, Dundas I, Bacterial dry matter content and biomass estimations. Appl Environ Microbiol1984;48:755–7. 6508285
25. NIHNIH Human Microbiome Project defines normal bacterial makeup of the body. Natl Institutes Heal2012. http://www.nih.gov/news-events/news-releases/nih-human-microbiome-project-defines-normal-bacterial-makeup-body (accessed January 3, 2015).
26. Goodsell DS, The Machinery of Life. New York, NY: Springer New York; 2009. doi: 10.1007/978-0-387-84925-6
27. Alberts B, Bray D, Lewis J, Raff M, Molecular biology of the cell. 4th ed.New York: Garland Science; 2002.
28. Azevedo F a C, Carvalho LRB, Grinberg LT, Farfel JM, Ferretti REL, Leite REP, et al. Equal numbers of neuronal and nonneuronal cells make the human brain an isometrically scaled-up primate brain. J Comp Neurol2009;513:532–41. doi: 10.1002/cne.2197419226510
29. Leggett RW, Williamst LR, Blood Volumes in Humans. Health Phys1991;60:139–54. 1989937
30. Burton C, Relation of Structure to function of the wall of blood vessels. Physiol Rev1954;34:619–42. 13215088
31. Félétou M, The endothelium: Part multiple functions of the endothelial cells—focus on endothelium-derived vasoactive mediators. San Rafael, CA: Morgan & Claypool Life Sciences; 2011.
32. Herman I, Physics of the human body. New York: Columbia University; 2007.
33. Randolph RK, Simon M, Dermal fibroblasts actively metabolize retinoic acid but not retinol. J Invest Dermatol1998;111:478–84. doi: 10.1046/j.1523-1747.1998.00307.x9740244
34. Moore TL, Lunt M, McManus B, Anderson ME, Herrick a L, Seventeen-point dermal ultrasound scoring system—a reliable measure of skin thickness in patients with systemic sclerosis. Rheumatology (Oxford)2003;42:1559–63. doi: 10.1093/rheumatology/keg435
35. McGrath JA, Eady RAJ, Pope FM, Anatomy and Organization of Human Skin. Rook’s Textb. Dermatology. 7th ed., Blackwell Publishing company; 2004, p. 3.33. doi: 10.1002/9780470750520
36. Miller CC, Godeau G, Lebreton-DeCoster C, Desmoulière a, Pellat B, Dubertret L, et al. Validation of a morphometric method for evaluating fibroblast numbers in normal and pathologic tissues. Exp Dermatol2003;12:403–11. doi: 10.1034/j.1600-0625.2003.00023.x12930296
37. Shen W, Pietrobelli A, Wang J, Wang Z, Heymsfield SB, Four-Compartment Cellular Level Body Composition Model: Comparison of Two Approaches. Obes Res2005;13:58–65. doi: 10.1038/oby.2005.815761163
38. Wang Z, St-Onge M-P, Lecumberri B, Pi-Sunyer FX, Heshka S, Wang J, et al. Body cell mass: model development and validation at the cellular level of body composition. Am J Physiol Endocrinol Metab2004;286:E123–8. doi: 10.1152/ajpendo.00227.200314532167
39. ICRP. Basic anatomical and physiological data for use in radiological protection: reference values. ICRP Publication 89. vol. 32. Pergamon; 2002. doi: 10.1016/S0146-6453(03)00002-2.
40. Roger LC, Mccartney AL, Longitudinal investigation of the faecal microbiota of healthy full-term infants using fluorescence in situ hybridization and denaturing gradient gel electrophoresis. Microbiology2010:3317–28. doi: 10.1099/mic.0.041913–020829292
41. Hopkins MJ, Macfarlane GT, Furrie E, Fite A, Macfarlane S, Characterisation of intestinal bacteria in infant stools using real-time PCR and northern hybridisation analyses. FEMS Microbiol Ecol2005;54:77–85. doi: 10.1016/j.femsec.2005.03.00116329974
42. Hansen R, Scott KP, Khan S, Martin JC, Berry SH, Stevenson M, et al. First-Pass Meconium Samples from Healthy Term Vaginally-Delivered Neonates: An Analysis of the Microbiota. PLoS ONE2015;15:1–10. doi: 10.1371/journal.pone.0133320
43. Vulevic J, Juric A, Tzortzis G, Gibson GR, A Mixture of trans -Galactooligosaccharides Reduces Markers of Metabolic Syndrome and Modulates the Fecal Microbiota and Immune Function of Overweight Adults 1–3. J Nutr2013:324–31. doi: 10.3945/jn.112.166132.galactooligosaccharides23303873
44. Davy KP, Seals DR, Total blood volume in healthy young and older men. Am Physiol Soc1994;76:2059–62.
45. Retzlaff JA, Tauxe WN, Kiel JM, Stroebel CF, Erythrocyte Volume, Plasma Volume, and Lean Body Mass in Adult Men and Women. Blood1969;33:649–67. 5779153
46. Young JF, Luecke RH, Pearce BA, Lee T, Ahn H, Baek S, et al. Human Organ / Tissue Growth Algorithms that Include Obese Individuals and Black / White Population Organ Weight Similarities from Autopsy Data. J Toxicol Environ Health2009;72:527–40. doi: 10.1080/15287390802647203
47. Suzuki TA, Worobey M, Geographical variation of human gut microbial composition. Biol Lett2014;10:20131037. doi: 10.1098/rsbl.2013.103724522631
48. Robin ED, Wong R, Mitochondrial DNA molecules and virtual number of mitochondria per cell in mammalian cells. J Cell Physiol1988;136:507–13. doi: 10.1002/jcp.10413603163170646
49. Tomasetti C, Vogelstein B, Variation in cancer risk among tissues can be explained by the number of stem cell divisions. Science2015;347:78–80. doi: 10.1126/science.126082525554788