Isolation of exosome-like vesicles from plants by ultracentrifugation on sucrose/deuterium oxide (D2O) density cushions

Methods in Molecular Biology

Volumn 1459, Issue , 2016, Pages 259-269

  Stanly, Christopher ^a   Fiume, Immacolata ^a   Capasso, Giovambattista ^b   Pocsfalvi, Gabriella ^a  

^a CNR   (Italy)

^b SECOND UNIVERSITY OF NAPLES   (Italy)

Author keywords

Apoplastic vesicles; Differential centrifugation; Exosome like vesicles; Extracellular vesicles; Isolation; Juice; Plant tissue; Purification; Sucrose gradient centrifugation; Ultracentrifugation

Indexed keywords

DEUTERIUM OXIDE; SUCROSE;

CENTRIFUGATION; CITRUS FRUIT; EXOSOME; EXOSOME LIKE VESICLE; FRUIT JUICE; LIPIDOMICS; PROTEOMICS; TRANSCRIPTOMICS; ULTRACENTRIFUGATION; CELL FRACTIONATION; METABOLISM; PLANT; PROCEDURES; SECRETORY VESICLE;

CELL FRACTIONATION; EXOSOMES; EXTRACELLULAR VESICLES; PLANTS; SECRETORY VESICLES; ULTRACENTRIFUGATION;

EID: 84988851367     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-3804-9_18     Document Type: Chapter

References (13)

1. Yáñez-Mó M, Siljander PR-M, Andreu Z, Zavec AB, Borràs FE, Buzas EI, Buzas K, Casal E, Cappello F, Carvalho J, Colás E, Cordeiro-da Silva A, Fais S, Falcon-Perez JM, Ghobrial IM, Giebel B, Gimona M, Graner M, Gursel I, Gursel M, Heegaard NHH, Hendrix A, Kierulf P, Kokubun K, Kosanovic M, Kralj-Iglic V, Krämer-Albers E-M, Laitinen S, Lässer C, Lener T, Ligeti E, Linē A, Lipps G, Llorente A, Lötvall J, Manček-Keber M, Marcilla A, Mittelbrunn M, Nazarenko I, Nolte-‘t Hoen ENM, Nyman TA, Driscoll L, Olivan M, Oliveira C, Pállinger É, del Portillo HA, Reventós J, Rigau M, Rohde E, Sammar M, Sánchez-Madrid F, Santarém N, Schallmoser K, Stampe Ostenfeld M, Stoorvogel W, Stukelj R, Van der Grein SG, Vasconcelos MH, Wauben MHM, De Wever O (2015) Biological properties of extracellular vesicles and their physiological functions. Journal of Extracellular Vesicles 4. doi: 10.3402/jev.v4.27066
2. Yoon YJ, Kim OY, Gho YS (2014) Extracellular vesicles as emerging intercellular communicasomes. BMB Rep 47(10):531-539. doi: 10.5483/BMBRep.2014.47.10.164
3. Regente M, Pinedo M, Elizalde M, de la Canal L (2012) Apoplastic exosome-like vesicles: a new way of protein secretion in plants? Plant Signal Behav 7(5):544-546
4. Song Y, Zhang C, Ge W, Zhang Y, Burlingame AL, Guo Y (2011) Identification of NaCl stress-responsive apoplastic proteins in rice shoot stems by 2D-DIGE. J Proteomics 74(7): 1045-1067
5. Regente M, Corti-Monzón G, Maldonado AM, Pinedo M, Jorrín J, de la Canal L (2009) Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins. FEBS Lett 583(20):3363-3366. doi: 10.1016/j.febslet.2009.09.041
6. Gonorazky G, Laxalt AM, Dekker HL, Rep M, Munnik T, Testerink C, de la Canal L (2012) Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids. Plant Biol 14(1):41-49. doi: 10.1111/ j.1438-8677.2011.00488.x
7. Ju S, Mu J, Dokland T, Zhuang X, Wang Q, Jiang H, Xiang X, Deng ZB, Wang B, Zhang L, Roth M, Welti R, Mobley J, Jun Y, Miller D, Zhang HG (2013) Grape exosome-like nanoparticles induce intestinal stem cells and protect mice from DSS-induced colitis. Mol Ther 21(7): 1345-1357
8. Wang Q, Zhuang X, Mu J, Deng Z-B, Jiang H, Zhang L, Xiang X, Wang B, Yan J, Miller D, Zhang H-G (2013) Delivery of therapeutic agents by nanoparticles made of grapefruitderived lipids. Nat Commun 4:1867. doi: 10.1038/ncomms2886
9. Reyes FC, Buono R, Otegui MS (2011) Plant endosomal trafficking pathways. Curr Opin Plant Biol 14(6):666-673. doi: 10.1016/j. pbi.2011.07.009
10. An Q, van Bel AJ, Huckelhoven R (2007) Do plant cells secrete exosomes derived from multivesicular bodies? Plant Signal Behav 2(1):4-7
11. Pocsfalvi G, Raj DAA, Fiume I, Vilasi A, Trepiccione F, Capasso G (2015) Urinary extracellular vesicles as reservoirs of altered proteins during the pathogenesis of polycystic kidney disease. Proteomics Clin Appl 9(5-6):552-567. doi: 10.1002/prca.201400199
12. Raj DA, Fiume I, Capasso G, Pocsfalvi G (2012) A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. Kidney Int 81(12):1263-1272. doi: 10.1038/ki.2012.25
13. Joosten MAJ (2012) Isolation of apoplastic fluid from leaf tissue by the vacuum infiltrationcentrifugation technique. In: Bolton MD, Thomma BPHJ (eds) Plant fungal pathogens, vol 835, Methods in molecular biology. Humana, New York, NY, pp 603-610. doi: 10.1007/978-1-61779-501-5_38