Preparation of High-quality Hematoxylin and Eosin-stained Sections from Rodent Mammary Gland Whole Mounts for Histopathologic Review

Toxicologic Pathology

Volumn 44, Issue 7, 2016, Pages 1059-1064

  Tucker, Deirdre K ^a,b   Foley, Julie F ^b   Hayes Bouknight, Schantel A ^c   Fenton, Suzanne E ^b  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

^b NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

^c CHARLES RIVER LABORATORIES   (United States)

Author keywords

breast; contralateral; development; mammary gland; pathology; whole mount

Indexed keywords

ENDOCRINE DISRUPTOR; EOSIN; HEMATOXYLIN;

ADULT; ADVERSE OUTCOME; ANIMAL TISSUE; ARTICLE; AUTOPSY; ENVIRONMENTAL EXPOSURE; FALSE NEGATIVE RESULT; FEMALE; HISTOLOGY; HISTOPATHOLOGY; MAMMARY GLAND; MOUSE; NONHUMAN; PRIORITY JOURNAL; RISK REDUCTION; STAINING; TISSUE SECTION; ANIMAL; PATHOLOGY; UDDER;

ANIMALS; EOSINE YELLOWISH-(YS); FEMALE; HEMATOXYLIN; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; MAMMARY GLANDS, ANIMAL; MICE;

EID: 84988719564     PISSN: 01926233     EISSN: 15331601     Source Type: Journal    
DOI: 10.1177/0192623316660769     Document Type: Article

References (10)

1. Davis B., Fenton S., (2013). Mammary gland. Haschek and Rousseaux's Handbook of Toxicologic Pathology. Elsevier Academic Press, New York.
2. Filgo A. J., Foley J. F., Puvanesarajah S., Borde A. R., Midkiff B. R., Reed C. E., Chappell V. A., Alexander L. B., Borde P. R., Troester M. A., Hayes Bouknight S. A., Fenton S. E., (In press). Mammary gland evaluation in juvenile toxicity studies: Temporal developmental patterns in the male and female Harlan Sprague Dawley Rat. Toxicol Pathol 44.
3. Hvid H., Thorup I., Oleksiewicz M., Sjogren I., Jensen H., (2010). An alternative method for preparation of tissue sections from the rat mammary gland. Exp Toxicol Pathol 63, 317-24. doi:10.1016/j.etp.2010.02.005.
4. Keane K. A., Parker G. A., Regan K. S., Picut C., Dixon D., Creasy D., Giri D., Hukkanen R. R., (2015). Scientific and Regulatory Policy Committee (SRPC) points to consider: Histopathology evaluation of the pubertal development and thyroid function assay (OPPTS 890.1450, OPPTS 890.1500) in rats to screen for endocrine disruptors. Toxicol Pathol 43, 1047-63. doi:10.1177/0192623315579943.
5. Makris S. L., (2011). Current assessment of the effects of environmental chemicals on the mammary gland in guideline rodent studies by the U.S. Environmental Protection Agency (EPA), Organisation for Economic Co-operation and Development (OECD), and National Toxicology Program (NTP). Environ Health Perspect 119, 1047-52.
6. National Toxicology Program. (2011). Specifications for the Conduct of Studies to Evaluate the Reproductive and Developmental Toxicity of Chemical, Biological, and Physical Agents in Laboratory Animals for the National Toxicology Program. National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, NC. Accessed March 18, 2016. http://ntp.niehs.nih.gov/ntp/test-info/finalntp-reprospecsmay2011-508.pdf.
7. OECD (Organisation for Economic Co-operation and Development). (2012). Test no. 443: Extended One-Generation Reproductive Toxicity Study. OECD Guidelines for the Testing of Chemicals, Section 4. OECD Publishing, Paris, France. doi:http://dx.doi.org/10.1787/9789264185371-en.
8. Rudel R. A., Fenton S. E., Ackerman J. M., Euling S. Y., Makris S. L., (2011). Environmental exposures and mammary gland development: State of the science, public health implications, and research recommendations. Environ Health Perspect 119, 1053-61.
9. U.S. Environmental Protection Agency. (2009a). EDSP Test Guidelines OPPTS 890.1450 Pubertal Development and Thyroid Function in the Intact Juvenile/Peripubertal Female Rats. EPA 740-C-09-009. U.S. Environmental Protection Agency, Washington, DC.
10. U.S. Environmental Protection Agency. (2009b). EDSP Test Guidelines OPPTS 890.1500 Pubertal Development and Thyroid Function in Intact Juvenile/Peripubertal Male Rats. EPA 740-C-09-012. U.S. Environmental Protection Agency, Washington, DC.