Nuclear actin modulates cell motility via transcriptional regulation of adhesive and cytoskeletal genes

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Sharili, Amir S ^a   Kenny, Fiona N ^a   Vartiainen, Maria K ^b   Connelly, John T ^a,c  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^b UNIVERSITY OF HELSINKI   (Finland)

^c QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; CELL ADHESION MOLECULE; NUCLEAR PROTEIN;

BIOSYNTHESIS; CYTOLOGY; CYTOSKELETON; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; HELA CELL LINE; HUMAN; KERATINOCYTE; METABOLISM; PHYSIOLOGY;

ACTINS; CELL ADHESION MOLECULES; CYTOSKELETON; GENE EXPRESSION REGULATION; HELA CELLS; HUMANS; KERATINOCYTES; NUCLEAR PROTEINS; TRANSCRIPTION, GENETIC;

EID: 84988632757     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep33893     Document Type: Article

References (30)

1. Gardel, M. L., Schneider, I. C., Aratyn-Schaus, Y. & Waterman, C. M. Mechanical integration of actin and adhesion dynamics in cell migration. Annu. Rev. Cell Dev. Biol. 26, 315-333 (2010).
2. Burnette, D. T. et al. A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells. J. Cell Biol. 205, 83-96 (2014).
3. Rungger, D., Rungger-Brandle, E., Chaponnier, C. & Gabbiani, G. Intranuclear injection of anti-actin antibodies into Xenopus oocytes blocks chromosome condensation. Nature 282, 320-321 (1979).
4. Scheer, U., Hinssen, H., Franke, W. W. & Jockusch, B. M. Microinjection of actin-binding proteins and actin antibodies demonstrates involvement of nuclear actin in transcription of lampbrush chromosomes. Cell 39, 111-122 (1984).
5. McDonald, D., Carrero, G., Andrin, C., De Vries, G. & Hendzel, M. J. Nucleoplasmic beta-actin exists in a dynamic equilibrium between low-mobility polymeric species and rapidly diffusing populations. J Cell Biol 172, 541-552 (2006).
6. Dopie, J., Skarp, K. P., Rajakyla, E. K., Tanhuanpaa, K. & Vartiainen, M. K. Active maintenance of nuclear actin by importin 9 supports transcription. Proc Natl Acad Sci USA 109, E544-E552 (2012).
7. Stuven, T., Hartmann, E. & Gorlich, D. Exportin 6: a novel nuclear export receptor that is specific for profilin. actin complexes. Embo J 22, 5928-5940 (2003).
8. Miyamoto, K., Pasque, V., Jullien, J. & Gurdon, J. B. Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes. Genes Dev. 25, 946-958 (2011).
9. Miyamoto, K. et al. Nuclear Wave1 is required for reprogramming transcription in oocytes and for normal development. Science 341, 1002-1005 (2013).
10. Baarlink, C., Wang, H. & Grosse, R. Nuclear actin network assembly by formins regulates the SRF coactivator MAL. Science 340, 864-867 (2013).
11. Huang, W. et al. Coronin 2A mediates actin-dependent de-repression of inflammatory response genes. Nature 470, 414-418 (2011).
12. Hofmann, W. A. et al. Actin is part of pre-initiation complexes and is necessary for transcription by RNA polymerase II. Nat Cell Biol 6, 1094-1101 (2004).
13. Philimonenko, V. V. et al. Nuclear actin and myosin I are required for RNA polymerase I transcription. Nat Cell Biol 6, 1165-1172 (2004).
14. Kapoor, P., Chen, M., Winkler, D. D., Luger, K. & Shen, X. Evidence for monomeric actin function in INO80 chromatin remodeling. Nat. Struct. Mol. Biol. 20, 426-432 (2013).
15. Rando, O. J., Zhao, K., Janmey, P. & Crabtree, G. R. Phosphatidylinositol-dependent actin filament binding by the SWI/SNF-like BAF chromatin remodeling complex. Proc. Natl. Acad. Sci. USA 99, 2824-2829 (2002).
16. Vartiainen, M. K., Guettler, S., Larijani, B. & Treisman, R. Nuclear actin regulates dynamic subcellular localization and activity of the SRF cofactor MAL. Science 316, 1749-1752 (2007).
17. Ho, C. Y., Jaalouk, D. E., Vartiainen, M. K. & Lammerding, J. Lamin A/C and emerin regulate MKL1-SRF activity by modulating actin dynamics. Nature 497, 507-511 (2013).
18. Spencer, V. A. et al. Depletion of nuclear actin is a key mediator of quiescence in epithelial cells. J Cell Sci 124, 123-132 (2010).
19. Sen, B. et al. Intranuclear Actin Regulates Osteogenesis. Stem Cells 33, 3065-3076 (2015).
20. Plessner, M., Melak, M., Chinchilla, P., Baarlink, C. & Grosse, R. Nuclear F-actin Formation and Reorganization upon Cell Spreading? . J Biol Chem 290, 11209-11216 (2015).
21. Brandt, D. T. et al. SCAI acts as a suppressor of cancer cell invasion through the transcriptional control of beta1-integrin. Nat Cell Biol 11, 557-568 (2009).
22. Gineitis, D. & Treisman, R. Differential usage of signal transduction pathways defines two types of serum response factor target gene. J Biol Chem 276, 24532-24539 (2001).
23. Medjkane, S., Perez-Sanchez, C., Gaggioli, C., Sahai, E. & Treisman, R. Myocardin-related transcription factors and SRF are required for cytoskeletal dynamics and experimental metastasis. Nat Cell Biol 11, 257-268 (2009).
24. Posern, G., Sotiropoulos, A. & Treisman, R. Mutant Actins Demonstrate a Role for Unpolymerized Actin in Control of Transcription by Serum Response Factor. Mol Biol Cell 13, 4167-4178 (2002).
25. Ponti, A., Machacek, M., Gupton, S. L., Waterman-Storer, C. M. & Danuser, G. Two distinct actin networks drive the protrusion of migrating cells. Science 305, 1782-1786 (2004).
26. Horsley, V., Aliprantis, A. O., Polak, L., Glimcher, L. H. & Fuchs, E. NFATc1 balances quiescence and proliferation of skin stem cells. Cell 132, 299-310 (2008).
27. Belin, B. J., Lee, T. & Mullins, R. D. DNA damage induces nuclear actin filament assembly by Formin-2 and Spire-1/2 that promotes efficient DNA repair. eLife 4, e07735 (2015).
28. Boukamp, P. et al. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J. Cell Biol. 106, 761-771 (1988).
29. Connelly, J. T. et al. Actin and serum response factor transduce physical cues from the microenvironment to regulate epidermal stem cell fate decisions. Nat Cell Biol 12, 711-718 (2010).
30. Miralles, F., Posern, G., Zaromytidou, A. I. & Treisman, R. Actin dynamics control SRF activity by regulation of its coactivator MAL. Cell 113, 329-342 (2003).