Platelets confound the measurement of extracellular miRNA in archived plasma

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Mitchell, Adam J ^a   Gray, Warren D ^a   Hayek, Salim S ^a   Ko, Yi An ^b   Thomas, Sheena ^a   Rooney, Kim ^a   Awad, Mosaab ^a   Roback, John D ^a   Quyyumi, Arshed ^a   Searles, Charles D ^a,c  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b EMORY UNIVERSITY   (United States)

^c ATLANTA VA MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MICRORNA;

BLOOD; FLOW CYTOMETRY; FREEZING; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MEMBRANE MICROPARTICLE; PLATELET COUNT; PROCEDURES; SPECIMEN HANDLING; THROMBOCYTE;

BLOOD PLATELETS; CELL-DERIVED MICROPARTICLES; FLOW CYTOMETRY; FREEZING; HUMANS; MICRORNAS; PLATELET COUNT; SPECIMEN HANDLING;

EID: 84987905085     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep32651     Document Type: Article

References (23)

1. Melman, Y. F. et al. Circulating MicroRNA-30d Is Associated With Response to Cardiac Resynchronization Therapy in Heart Failure, and Regulates Cardiomyocyte Apoptosis: A Translational Pilot Study. Circulation 131, 2202-2216, doi: 10.1161/ CIRCULATIONAHA.114.013220 (2015
2. Zampetaki, A. et al. Prospective study on circulating MicroRNAs, and risk of myocardial infarction. Journal of the American College of Cardiology 60, 290-299, doi: 10.1016/j.jacc.2012.03.056 (2012
3. Zampetaki, A. et al. Plasma microRNA profiling reveals loss of endothelial miR-126, and other microRNAs in type 2 diabetes. Circulation research 107, 810-817, doi: 10.1161/CIRCRESAHA.110.226357 (2010
4. Fichtlscherer, S. et al. Circulating microRNAs in patients with coronary artery disease. Circulation research 107, 677-684, doi: 10.1161/CIRCRESAHA.109.215566 (2010
5. Ellis, K. L. et al. Circulating microRNAs as candidate markers to distinguish heart failure in breathless patients. European journal of heart failure 15, 1138-1147, doi: 10.1093/eurjhf/hft078 (2013
6. Boeri, M. et al. MicroRNA signatures in tissues, and plasma predict development, and prognosis of computed tomography detected lung cancer. Proceedings of the National Academy of Sciences of the United States of America 108, 3713-3718, doi: 10.1073/pnas.1100048108 (2011
7. Cheng, H. H. et al. Plasma processing conditions substantially influence circulating microRNA biomarker levels. PloS one 8, e64795, doi: 10.1371/journal.pone.0064795 (2013
8. Lacroix, R. et al. Impact of pre-Analytical parameters on the measurement of circulating microparticles: towards standardization of protocol. Journal of thrombosis, and haemostasis: JTH 10, 437-446, doi: 10.1111/j.1538-7836.2011.04610.x (2012
9. Ple, H. et al. The repertoire, and features of human platelet microRNAs. PloS one 7, e50746, doi: 10.1371/journal.pone.0050746 (2012
10. Kaudewitz, D. et al. Association of MicroRNAs, and YRNAs With Platelet Function. Circulation research 118, 420-432, doi: 10.1161/CIRCRESAHA.114.305663 (2016
11. Reid, T. J., Esteban, G., Clear, M., & Gorogias, M. Platelet membrane integrity during storage, and activation. Transfusion 39, 616-624 (1999
12. Reid, T. J. et al. Cooling, and freezing damage platelet membrane integrity. Cryobiology 38, 209-224, doi: 10.1006/cryo.1999.2164 (1999
13. McShine, R. L., Sibinga, S., & Brozovic, B. Differences between the effects of EDTA, and citrate anticoagulants on platelet count, and mean platelet volume. Clin Lab Haematol 12, 277-285 (1990
14. Mullier, F., Bailly, N., Chatelain, C., Chatelain, B., & Dogne, J. M. Pre-Analytical issues in the measurement of circulating microparticles: current recommendations, and pending questions. Journal of thrombosis, and haemostasis: JTH 11, 693-696, doi: 10.1111/jth.12171 (2013
15. Gyorgy, B. et al. Improved circulating microparticle analysis in acid-citrate dextrose (ACD) anticoagulant tube. Thromb Res 133, 285-292, doi: 10.1016/j.thromres.2013.11.010 (2014
16. Lacroix, R. et al. Standardization of pre-Analytical variables in plasma microparticle determination: results of the International Society on Thrombosis, and Haemostasis SSC Collaborative workshop. Journal of thrombosis, and haemostasis: JTH, doi: 10.1111/jth.12207 (2013
17. Rayner, K. J., & Hennessy, E. J. Extracellular communication via microRNA: lipid particles have a new message. J Lipid Res 54, 1174-1181, doi: 10.1194/jlr.R034991 (2013
18. Christersson, C., Johnell, M., & Siegbahn, A. Evaluation of microparticles in whole blood by multicolour flow cytometry assay. Scand J Clin Lab Invest 73, 229-239, doi: 10.3109/00365513.2013.769278 (2013
19. Poole, J. et al. Effect of progenitor cell mobilization with granulocyte-macrophage colony-stimulating factor in patients with peripheral artery disease: a randomized clinical trial. Jama 310, 2631-2639, doi: 10.1001/jama.2013.282540 (2013
20. Gray, W. D., Mitchell, A. J., & Searles, C. D. An accurate, precise method for general labeling of extracellular vesicles. MethodsX 2, 360-367, doi: 10.1016/j.mex.2015.08.002 (2015
21. Marabita, F. et al. Normalization of circulating microRNA expression data obtained by quantitative real-Time RT-PCR. Briefings in bioinformatics, doi: 10.1093/bib/bbv056 (2015
22. McDermott, A. M., Kerin, M. J., & Miller, N. Identification, and validation of miRNAs as endogenous controls for RQ-PCR in blood specimens for breast cancer studies. PloS one 8, e83718, doi: 10.1371/journal.pone.0083718 (2013
23. Chang, K. H., Mestdagh, P., Vandesompele, J., Kerin, M. J., & Miller, N. MicroRNA expression profiling to identify, and validate reference genes for relative quantification in colorectal cancer. BMC cancer 10, 173, doi: 10.1186/1471-2407-10-173 (2010