Simplified development of multiplex real-time PCR through master mix augmented by universal fluorogenic reporters

BioTechniques

Volumn 61, Issue 3, 2016, Pages 123-128

  Wadle, Simon ^a   Lehnert, Michael ^a   Schuler, Friedrich ^a,b   Köppel, René ^c   Serr, Annerose ^d   Zengerle, Roland ^a,b   von Stetten, Felix ^a,b  

^a UNIVERSITY OF FREIBURG   (Germany)

^b Hahn Schickard   (Germany)

^c Official Food Control Authority of the Canton of Zurich   (Switzerland)

^d UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Food panel; Mediator probe PCR; MRSA; Multiplex PCR; Universal reporter

Indexed keywords

DNA PROBE; HUMAN; HYDROLYSIS; LIMIT OF DETECTION; MEDIATOR; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; ANIMAL; CHEMISTRY; CLASSIFICATION; FOOD; FOOD ANALYSIS; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR TYPING; PROCEDURES; REPRODUCIBILITY;

DNA; FLUORESCENT DYE;

ANIMALS; DNA; FLUORESCENT DYES; FOOD; FOOD ANALYSIS; LIMIT OF DETECTION; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MOLECULAR TYPING; MULTIPLEX POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS;

EID: 84986910762     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000114443     Document Type: Article

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