Real-time PCR probe optimization using design of experiments approach

Biomolecular Detection and Quantification

Volumn 7, Issue , 2016, Pages 1-8

  Wadle, S ^a,b   Lehnert, M ^a   Rubenwolf, S ^a   Zengerle, R ^a,b   von Stetten, F ^a,b  

^a UNIVERSITY OF FREIBURG   (Germany)

^b HSG IMIT Institute für Mikro and Information Technology   (Germany)

Author keywords

Design of experiments; Mediator probe PCR; PCR optimization; Real time PCR; Universal reporter

Indexed keywords

D DIMER; DEOXYRIBONUCLEASE I; TAQ POLYMERASE;

ARTICLE; BINDING SITE; FLUORESCENCE; GENE DOSAGE; GENE SEQUENCE; HUMAN METAPNEUMOVIRUS; LIMIT OF DETECTION; MATHEMATICAL COMPUTING; MECHANICAL PROBE; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL DETECTION; TEMPERATURE;

EID: 84951955594     PISSN: None     EISSN: 22147535     Source Type: Journal    
DOI: 10.1016/j.bdq.2015.12.002     Document Type: Article

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