From clinical sample to complete genome: Comparing methods for the extraction of HIV-1 RNA for high-throughput deep sequencing

Virus Research

Volumn 239, Issue , 2017, Pages 10-16

  Cornelissen, Marion ^a   Gall, Astrid ^b   Vink, Monique ^a   Zorgdrager, Fokla ^a   Binter, Špela ^b   Edwards, Stephanie ^b   Jurriaans, Suzanne ^a   Bakker, Margreet ^a   Ong, Swee Hoe ^b   Gras, Luuk ^c   van Sighem, Ard ^c   Bezemer, Daniela ^c   de Wolf, Frank ^d   Reiss, Peter ^a,c,e   Kellam, Paul ^b   Berkhout, Ben ^a   Fraser, Christophe ^d   van der Kuyl, Antoinette C ^a  

^a ACADEMIC MEDICAL CENTER   (Netherlands)

^b WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^c STICHTING HIV MONITORING   (Netherlands)

^d IMPERIAL COLLEGE LONDON   (United Kingdom)

^e AMSTERDAM INSTITUTE FOR GLOBAL HEALTH AND DEVELOPMENT   (Netherlands)

Author keywords

Complete genome; High throughput deep sequencing; HIV 1; QIAamp viral isolation kit; RNA isolation

Indexed keywords

VIRUS RNA;

AMPLICON; ARTICLE; GENE AMPLIFICATION; GENE SEQUENCE; HIGH THROUGHPUT SEQUENCING; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; INTERMETHOD COMPARISON; NONHUMAN; PILOT STUDY; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA DEGRADATION; RNA EXTRACTION; RNA ISOLATION; VIRUS GENOME; VIRUS LOAD; CLASSIFICATION; GENETICS; GENOMICS; GENOTYPE; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; ISOLATION AND PURIFICATION; PROCEDURES; VIROLOGY; WHOLE GENOME SEQUENCING;

GENOME, VIRAL; GENOMICS; GENOTYPE; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HIV INFECTIONS; HIV-1; HUMANS; RNA, VIRAL; VIRAL LOAD; WHOLE GENOME SEQUENCING;

EID: 84981747131     PISSN: 01681702     EISSN: 18727492     Source Type: Journal    
DOI: 10.1016/j.virusres.2016.08.004     Document Type: Article

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