Human placental and decidual organ cultures to study infections at the maternal-fetal interface

Journal of Visualized Experiments

Volumn 2016, Issue 113, 2016, Pages

  Rizzuto, Gabrielle A ^a,b   Kapidzic, Mirhan ^b   Gormley, Matthew ^b   Bakardjiev, Anna I ^a,c  

^a Benioff Children s Hospital   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Human decidua; Human placenta; Immunology; Issue 113; Listeria monocytogenes; Organ culture; Pathogenesis

Indexed keywords

DECIDUA; FEMALE; HOST PATHOGEN INTERACTION; HUMAN; LISTERIA MONOCYTOGENES; ORGAN CULTURE TECHNIQUE; PHYSIOLOGY; PLACENTA; PREGNANCY;

DECIDUA; FEMALE; HOST-PATHOGEN INTERACTIONS; HUMANS; LISTERIA MONOCYTOGENES; ORGAN CULTURE TECHNIQUES; PLACENTA; PREGNANCY;

EID: 84979901941     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/54237     Document Type: Article

References (28)

1. Pan, X., Yang, Y., & Zhang, J. R. Molecular basis of host specificity in human pathogenic bacteria. Emerging microbes & infections. 3, e23 (2014).
2. Robbins, J. R., Skrzypczynska, K. M., Zeldovich, V. B., Kapidzic, M., & Bakardjiev, A. I. Placental syncytiotrophoblast constitutes a major barrier to vertical transmission of Listeria monocytogenes. PLoS pathogens. 6, e1000732 (2010).
3. Zhang, J. R. et al. The polymeric immunoglobulin receptor translocates pneumococci across human nasopharyngeal epithelial cells. Cell. 102, 827-837 (2000).
4. Ngampasutadol, J. et al. Human C4b-binding protein selectively interacts with Neisseria gonorrhoeae and results in species-specific infection. Proceedings of the National Academy of Sciences of the United States of America. 102, 17142-17147 (2005).
5. Pishchany, G. et al. Specificity for human hemoglobin enhances Staphylococcus aureus infection. Cell host & microbe. 8, 544-550 (2010).
6. Benirschke, K., Kaufmann, P., Baergen, R. N. Pathology of the Human Placenta. Springer-Verlag Berlin Heidelberg (2012).
7. Robbins, J. R., Zeldovich, V. B., Poukchanski, A., Boothroyd, J. C., & Bakardjiev, A. I. Tissue barriers of the human placenta to infection with Toxoplasma gondii. Infection and immunity. 80, 418-428 (2012).
8. Shamir, E. R., & Ewald, A. J. Three-dimensional organotypic culture: experimental models of mammalian biology and disease. Nature reviews. Molecular cell biology. 15, 647-664 (2014).
9. Hazan, A. D. et al. Vascular-leukocyte interactions: mechanisms of human decidual spiral artery remodeling in vitro. The American journal of pathology. 177, 1017-1030 (2010).
10. Dunk, C. et al. A novel in vitro model of trophoblast-mediated decidual blood vessel remodeling. Laboratory investigation; a journal of technical methods and pathology. 83, 1821-1828 (2003).
11. Hunkapiller, N. M., & Fisher, S. J. Chapter 12. Placental remodeling of the uterine vasculature. Methods in enzymology. 445, 281-302 (2008).
12. Miller, J. M. et al. Guidelines for safe work practices in human and animal medical diagnostic laboratories. Recommendations of a CDCconvened, Biosafety Blue Ribbon Panel. MMWR Surveill Summ. 61 Suppl, 1-102 (2012).
13. Lash, G. E. et al. Low oxygen concentrations inhibit trophoblast cell invasion from early gestation placental explants via alterations in levels of the urokinase plasminogen activator system. Biol Reprod. 74, 403-409 (2006).
14. Wang, N., Strugnell, R., Wijburg, O., & Brodnicki, T. Measuring bacterial load and immune responses in mice infected with Listeria monocytogenes. Journal of visualized experiments: JoVE. (2011).
15. Palmer, M. E., Watson, A. L., & Burton, G. J. Morphological analysis of degeneration and regeneration of syncytiotrophoblast in first trimester placental villi during organ culture. Hum Reprod. 12, 379-382 (1997).
16. Elsinghorst, E. A. Measurement of invasion by gentamicin resistance. Methods in enzymology. 236, 405-420 (1994).
17. Kucherenko, M. M. et al. Paraffin-embedded and frozen sections of Drosophila adult muscles. Journal of visualized experiments: JoVE. (2010).
18. Croy, B. A., Yamada, A. T., DeMayo, F. J., Adamson, S. L. The Guide to Investigation of Mouse Pregnancy, 1st Edition. (2014).
19. JoVE Science Education Database. General Laboratory Techniques. Histological Sample Preparation for Light Microscopy. JoVE, Cambridge, MA (2016).
20. Troy, T. C., Arabzadeh, A., Enikanolaiye, A., Lariviere, N., & Turksen, K. Immunohistochemistry on paraffin sections of mouse epidermis using fluorescent antibodies. Journal of visualized experiments: JoVE. (2008).
21. Chi, V., & Chandy, K. G. Immunohistochemistry: paraffin sections using the Vectastain ABC kit from vector labs. Journal of visualized experiments: JoVE. e308 (2007).
22. Zeldovich, V. B., & Bakardjiev, A. I. Host defense and tolerance: unique challenges in the placenta. PLoS pathogens. 8, e1002804 (2012).
23. Yue, F. et al. A comparative encyclopedia of DNA elements in the mouse genome. Nature. 515, 355-364 (2014).
24. Robbins, J. R., & Bakardjiev, A. I. Pathogens and the placental fortress. Current opinion in microbiology. 15, 36-43 (2012).
25. Mestas, J., & Hughes, C. C. Of mice and not men: differences between mouse and human immunology. J Immunol. 172, 2731-2738 (2004).
26. Redline, R. W., Shea, C. M., Papaioannou, V. E., & Lu, C. Y. Defective anti-listerial responses in deciduoma of pseudopregnant mice. The American journal of pathology. 133, 485-497 (1988).
27. Redline, R. W., McKay, D. B., Vazquez, M. A., Papaioannou, V. E., & Lu, C. Y. Macrophage functions are regulated by the substratum of murine decidual stromal cells. The Journal of clinical investigation. 85, 1951-1958 (1990).
28. Bakardjiev, A. I., Theriot, J. A., & Portnoy, D. A. Listeria monocytogenes traffics from maternal organs to the placenta and back. PLoS pathogens. 2, e66 (2006).