Biosafety of biotechnologically important microalgae: Intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803

Biology Open

Volumn 5, Issue 4, 2016, Pages 519-528

  Elešnik, Helena Č ^a   Tanšek, Anja ^a   Tahirović, Aneja ^a   Vižintin, Angelika ^a   Mustar, Jernej ^a   Vidmar, Vita ^a   Dolinar, Marko ^a  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

Biocontainment; Biosafety; Cyanobacteria; Nuclease nuca; Synechocystis; Toxin antitoxin

Indexed keywords

ANTITOXIN; METAL ION;

ALGAL GROWTH; ANABAENA; ARTICLE; BIOSAFETY; BIOTECHNOLOGY; CELL KILLING; CONTROLLED STUDY; DNA DEGRADATION; GENE FUSION; GENE TRANSFER; GENETIC VARIABILITY; LETHALITY; NONHUMAN; PROMOTER REGION; SUICIDE; SYNECHOCYSTIS; SYNECHOCYSTIS SP PCC 6803;

EID: 84978393922     PISSN: None     EISSN: 20466390     Source Type: Journal    
DOI: 10.1242/bio.017129     Document Type: Article

References (48)

1. Ahrenholtz, I., Lorenz, M. G. and Wackernagel, W. (1994). A conditional suicide system in Escherichia coli based on the intracellular degradation of DNA. Appl. Environ. Microbiol. 60, 3746-3751.
2. Balan, A. and Schenberg, A. C. G. (2005). A conditional suicide system for Saccharomyces cerevisiae relying on the intracellular production of the Serratia marcescens nuclease. Yeast 22, 203-212.
3. Bej, A. K., Perlin, M. H. and Atlas, R. M. (1988). Model suicide vector for containment of genetically engineered microorganisms. Appl. Environ. Microbiol. 54, 2472-2477.
4. Blasi, B., Peca, L., Vass, I. and Kos, P. B. (2012). Characterization of stress responses of heavy metal and metalloid inducible promoters in synechocystis PCC6803. J. Microbiol. Biotechnol. 22, 166-169.
5. Briggs, L. M., Pecoraro, V. L. and McIntosh, L. (1990). Copper-induced expression, cloning, and regulatory studies of the plastocyanin gene from the cyanobacterium Synechocystis sp. PCC 6803. Plant Mol. Biol. 15, 633-642.
6. Cheah, Y. E., Albers, S. C. and Peebles, C. A. M. (2013). A novel counter-selection method for markerless genetic modification in Synechocystis sp. PCC 6803. Biotechnol. Prog. 29, 23-30.
7. Garcia-Dominguez, M., Lopez-Maury, L., Florencio, F. J. and Reyes, J. C. (2000). A gene cluster involved in metal homeostasis in the cyanobacterium Synechocystis sp. strain PCC 6803. J. Bacteriol. 182, 1507-1514.
8. Giner-Lamia, J., Lopez-Maury, L., Reyes, J. C. and Florencio, F. J. (2012). The CopRS two-component system is responsible for resistance to copper in the cyanobacterium Synechocystis sp. PCC 6803. Plant Physiol. 159, 1806-1818.
9. Giner-Lamia, J., Lopez-Maury, L. and Florencio, F. J. (2015). CopM is a novel copper-binding protein involved in copper resistance in Synechocystis sp. PCC 6803. Microbiol. Open 4, 167-185.
10. Guerrero, F., Carbonell, V., Cossu, M., Correddu, D. and Jones, P. R. (2012). Ethylene synthesis and regulated expression of recombinant protein in Synechocystis sp. PCC 6803. PLoS ONE 7, e50470.
11. Heidorn, T., Camsund, D., Huang, H.-H., Lindberg, P., Oliveira, P., Stensjo, K. and Lindblad, P. (2011). Synthetic biology in cyanobacteria engineering and analyzing novel functions. Methods Enzymol. 497, 539-579.
12. Huang, H. H., Camsund, D., Lindblad, P. and Heidorn, T. (2010). Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology. Nucleic Acids Res. 38, 2577-2593.
13. Jensen, L. B., Ramos, J. L., Kaneva, Z. and Molin, S. (1993). A substratedependent biological containment system for Pseudomonas putida based on the Escherichia coli gef gene. Appl. Environ. Microbiol. 59, 3713-3717.
14. Knudsen, S. M. and Karlstrom, O. H. (1991). Development of efficient suicide mechanisms for biological containment of bacteria. Appl. Environ. Microbiol. 57, 85-92.
15. Knudsen, S., Saadbye, P., Hansen, L. H., Collier, A., Jacobsen, B. L., Schlundt, J. and Karlstrom, O. H. (1995). Development and testing of improved suicide functions for biological containment of bacteria. Appl. Environ. Microbiol. 61, 985-991.
16. Kristoffersen, P., Jensen, G. B., Gerdes, K. and Piskur, J. (2000). Bacterial toxinantitoxin gene system as containment control in yeast cells. Appl. Environ. Microbiol. 66, 5524-5526.
17. Kuchmina, E., Wallner, T., Kryazhov, S., Zinchenko, V. V. and Wilde, A. (2012). An expression system for regulated protein production in Synechocystis sp. PCC 6803 and its application for construction of a conditional knockout of the ferrochelatase enzyme. J. Biotechnol. 162, 75-80.
18. Lai, M. C. and Lan, E. I. (2015). Advances in metabolic engineering of cyanobacteria for photosynthetic biochemical production. Metabolites 5, 636-658.
19. Landry, B. P., Stockel, J. and Pakrasi, H. B. (2013). Use of degradation tags to control protein levels in the Cyanobacterium Synechocystis sp. Strain PCC 6803. Appl. Environ. Microbiol. 79, 2833-2835.
20. Lau, N.-S., Matsui, M. and Abdullah, A. A.-A. (2015). Cyanobacteria: photoautotrophic microbial factories for the sustainable synthesis of industrial products. BioMed Res. Int. 2015, 754934.
21. Li, J. and Song, L. (2007). Applicability of the MTT assay for measuring viability of cyanobacteria and algae, specifically for Microcystis aeruginosa (Chroococcales, Cyanobacteria). Phycologia 46, 593-599.
22. Li, P., Harding, S. E. and Liu, Z. (2001). Cyanobacterial exopolysaccharides: their nature and potential biotechnological applications. Biotechnol. Genet. Eng. Rev. 18, 375-404.
23. Liu, X. and Curtiss, R.III. (2009). Nickel-inducible lysis system in Synechocystis sp. PCC 6803. Proc. Natl. Acad. Sci. USA 106, 21550-21554.
24. Lopez-Maury, L., Garcia-Dominguez, M., Florencio, F. J. and Reyes, J. C. (2002). A two-component signal transduction system involved in nickel sensing in the cyanobacterium Synechocystis sp. PCC 6803. Mol. Microbiol. 43, 247-256.
25. Makarova, K. S., Wolf, Y. I. and Koonin, E. V. (2009). Comprehensive comparativegenomic analysis of type 2 toxin-antitoxin systems and related mobile stress response systems in prokaryotes. Biol. Direct 4, 19.
26. Meiss, G., Franke, I., Gimadutdinow, O., Urbanke, C. and Pingoud, A. (1998). Biochemical characterization of Anabaena sp. strain PCC 7120 non-specific nuclease NucA and its inhibitor NuiA. Eur. J. Biochem. 251, 924-934.
27. Miller, J. H. (1972). Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
28. Miyake, K., Abe, K., Ferri, S., Nakajima, M., Nakamura, M., Yoshida, W., Kojima, K., Ikebukuro, K. and Sode, K. (2014). A green-light inducible lytic system for cyanobacterial cells. Biotechnol. Biofuels 7, 56.
29. Moe-Behrens, G. H. G., Davis, R. and Haynes, K. A. (2013). Preparing synthetic biology for the world. Front. Microbiol. 4, 5.
30. Morita, M., Asami, K., Tanji, Y. and Unno, H. (2001). Programmed Escherichia coli cell lysis by expression of cloned T4 phage lysis genes. Biotechnol. Prog. 17, 573-576.
31. Munthali, M. T., Timmis, K. N. and Diaz, E. (1996). Use of colicin e3 for biological containment of microorganisms. Appl. Environ. Microbiol. 62, 1805-1807.
32. Muro-Pastor, A. M., Flores, E., Herrero, A. and Wolk, C. P. (1992). Identification, genetic analysis and characterization of a sugar-non-specific nuclease from the cyanobacterium Anabaena sp. PCC 7120. Mol. Microbiol. 6, 3021-3030.
33. Mutalik, V. K., Guimaraes, J. C., Cambray, G., Lam, C., Christoffersen, M. J., Mai, Q.-A., Tran, A. B., Paull, M., Keasling, J. D., Arkin, A. P. et al. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements. Nat. Methods 10, 354-360.
34. Ning, D., Ye, S., Liu, B. and Chang, J. (2011). The proteolytic activation of the relNEs (ssr1114/slr0664) toxin-antitoxin system by both proteases Lons and ClpP2s/Xs of Synechocystis sp. PCC 6803. Curr. Microbiol. 63, 496-502.
35. Otsuka, Y. (2016). Prokaryotic toxin-antitoxin systems: novel regulations of the toxins. Curr. Genet. [Epub ahead of print]. doi:10.1007/s00294-015-0557-z.
36. Peca, L., Kos, P. and Vass, I. (2007). Characterization of the activity of heavy metalresponsive promoters in the cyanobacterium Synechocystis PCC 6803. Acta Biol. Hungarica 58 Suppl., 11-22.
37. Peca, L., Kos, P. B., Mate, Z., Farsang, A. and Vass, I. (2008). Construction of bioluminescent cyanobacterial reporter strains for detection of nickel, cobalt and zinc. FEMS Microbiol. Lett. 289, 258-264.
38. Pereira, S., Micheletti, E., Zille, A., Santos, A., Moradas-Ferreira, P., Tamagnini, P. and De Philippis, R. (2011). Using extracellular polymeric substances (EPS)-producing cyanobacteria for the bioremediation of heavy metals: do cations compete for the EPS functional groups and also accumulate inside the cell? Microbiology 157, 451-458.
39. Pinto, F., Pacheco, C. C., Ferreira, D., Moradas-Ferreira, P. and Tamagnini, P. (2012). Selection of suitable reference genes for RT-qPCR analyses in cyanobacteria. PLoS ONE 7, e34983.
40. Rippka, R. (1988). Isolation and purification of cyanobacteria. Methods Enzymol. 167, 3-27.
41. Schweder, T., Schmidt, I., Herrmann, H., Neubauer, P., Hecker, M. and Hofmann, K. (1992). An expression vector system providing plasmid stability and conditional suicide of plasmid-containing cells. Appl. Microbiol. Biotechnol. 38, 91-93.
42. Smith, A. B., Lopez-Villarejo, J., Diago-Navarro, E., Mitchenall, L. A., Barendregt, A., Heck, A. J., Lemonnier, M., Maxwell, A. and Diaz-Orejas, R. (2012). A common origin for the bacterial toxin-antitoxin systems parD and ccd, suggested by analyses of toxin/target and toxin/antitoxin interactions. PLoS ONE 7, e46499.
43. Torres, B., Jaenecke, S., Timmis, K. N., Garcia, J. L. and Diaz, E. (2003). A dual lethal system to enhance containment of recombinant micro-organisms. Microbiology 149, 3595-3601.
44. Turner, J. S., Glands, P. D., Samson, A. C. R. and Robinson, N. J. (1996). Zn2+ -sensing by the cyanobacterial metallothionein repressor SmtB: different motifs mediate metal-induced protein-DNA dissociation. Nucleic Acids Res. 24, 3714-3721.
45. Vioque, A. (2007). Transformation of cyanobacteria. Adv. Exp. Med. Biol. 616, 12-22.
46. Wright, O., Stan, G.-B. and Ellis, T. (2013). Building-in biosafety for synthetic biology. Microbiology 159, 1221-1235.
47. Wright, O., Delmans, M., Stan, G.-B. and Ellis, T. (2015). GeneGuard: a modular plasmid system designed for biosafety. ACS Synth. Biol. 4, 307-316.
48. Ye, S. and Ning, D. (2010). [The interaction between chromosome-encoded toxin Slr0664 and antitoxin Slr1114 of cyanobacteria Synechocystis sp. PCC6803]. Wei Sheng Wu Xue Bao 50, 743-748.