A novel counter-selection method for markerless genetic modification in Synechocystis sp. PCC 6803

Biotechnology Progress

Volumn 29, Issue 1, 2013, Pages 23-30

  Cheah, Yi Ern ^a   Albers, Stevan C ^b   Peebles, Christie A M ^a,b  

^a Department of Mechanical Engineering   (United States)

^b Veterinary Dentistry and Oral Surgery   (United States)

Author keywords

Cyanobacteria; Genetic engineering; Homologous recombination; Markerless transformation; MazF; Nickel response system; Synechocystis

Indexed keywords

CYANOBACTERIA; HOMOLOGOUS RECOMBINATION; MARKERLESS; MAZF; RESPONSE SYSTEMS; SYNECHOCYSTIS;

CARBON DIOXIDE; GENES; NICKEL;

GENETIC ENGINEERING;

BACTERIAL DNA; DNA BINDING PROTEIN; ESCHERICHIA COLI PROTEIN; MAZE PROTEIN, E COLI;

ARTICLE; ESCHERICHIA COLI; GENETIC ENGINEERING; GENETIC VARIABILITY; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; METHODOLOGY; SYNECHOCYSTIS;

DNA, BACTERIAL; DNA-BINDING PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC ENGINEERING; GENETIC VARIATION; SYNECHOCYSTIS;

CYANOBACTERIA; SYNECHOCYSTIS; SYNECHOCYSTIS SP.; SYNECHOCYSTIS SP. PCC 6803;

EID: 84873313528     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1661     Document Type: Article

References (23)

1. Ermakova-Gerdes S, Shestakov S, Vermaas W. Random chemical mutagenesis of a specific psbDI region coding for a lumenal loop of the D2 protein of photosystem II in Synechocystis sp PCC 6803. Plant Mol Biol. 1996; 30: 243-254.
2. Vermaas WFJ, Ikeuchi M, Inoue Y. Protein-composition of the photosystem-ii core complex in genetically engineered mutants of the cyanobacterium Synechocystis sp PCC-6803. Photo Res. 1988; 17: 97-113.
3. Jeanjean R, Zuther E, Yeremenko N, Havaux M, Matthijs HCP. Hagemann M. A photosystem 1 psaFJ-null mutant of the cyanobacterium Synechocystis PCC 6803 expresses the isiAB operon under iron replete conditions. FEBS Lett. 2003; 549: 52-56.
4. Kaneko T, Tabata S. Complete genome structure of the unicellular cyanobacterium Synechocystis sp. PCC6803. Plant Cell Physiol. 1997; 38: 1171-1176.
5. Koksharova OA, Wolk CP. Genetic tools for cyanobacteria. Appl Microbiol Biot. 2002; 58: 123-137.
6. Robertson DE, Jacobson SA, Morgan F, Berry D, Church GM, Afeyan NB. A new dawn for industrial photosynthesis. Photosyn Res. 2011; 107: 269-277.
7. Williams JGK. Construction of specific mutations in photosystem-ii photosynthetic reaction center by genetic-engineering methods in Synechocystis-6803. Meth Enzymol. 1988; 167: 766-778.
8. Lagarde D, Beuf L, Vermaas M. Increased production of zeaxanthin and other pigments by application of genetic engineering techniques to Synechocystis sp strain PCC 6803. Appl Environ Microb. 2000; 66: 64-72.
9. Liu XY, Sheng J, Curtiss R. Fatty acid production in genetically modified cyanobacteria. Prot Natl Acad Sci USA. 2011; 108: 6899-6904.
10. Zhang YL, Zhang JJ, Hoeflich KP, Ikura M, Qing GL, Inouye M. MazF cleaves cellular mRNAs specifically at ACA to block protein synthesis in Escherichia coli. Mol Cell. 2003; 12: 913-923.
11. Xu Y, Alvey RM, Byrne PO, Graham JE, Shen G, Bryant DA, Carpentier R. Expression of genes in cyanobacteria: Adaptation of endogenous plasmids as platforms for high-level gene expression in Synechococcus sp. PCC 7002. Methods Mol Biol. 2011; 684: 273-293.
12. Kilian O, Benemann CSE, Niyogi KK, Vick B. High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp. P Natl Acad Sci USA. 2011; 108: 21265-21269.
13. Julian E-R. The construction of gene knockouts in the cyanobacterium Synechocystis sp. PCC 6803. Methods Mol Biol. 2004; 274: 309-324.
14. Kunert A, Hagemann M, Erdmann N. Construction of promoter probe vectors for Synechocystis sp PCC 6803 using the light-emitting reporter systems Gfp and LuxAB. J Microbiol Meth. 2000; 41: 185-194.
15. Lopez-Maury L, Garcia-Dominguez M, Florencio FJ, Reyes JC. A two-component signal transduction system involved in nickel sensing in the cyanobacterium Synechocystis sp PCC 6803. Mol Microbiol. 2002; 43: 247-256.
16. Taroncher-Oldenberg G, Nishina K, Stephanopoulos G. Identification and analysis of the polyhydroxyalkanoate-specific beta-ketothiolase and acetoacetyl coenzyme A reductase genes in the cyanobacterium Synechocystis sp strain PCC6803. Appl Environ Microb. 2000; 66: 4440-4448.
17. Gay P, Lecoq D, Steinmetz M, Ferrari E, Hoch JA. Cloning structural gene SacB, which codes for exoenzyme levansucrase of Bacillus-subtilis - expression of the gene in Escherichia-coli. J Bacteriol. 1983; 153: 1424-1431.
18. Gay P, Lecoq D, Steinmetz M, Berkelman T, Kado CI. Positive selection procedure for entrapment of insertion-sequence elements in gram-negative bacteria. J Bacteriol. 1985; 164: 918-921.
19. Zhang XZ, Yan X, Cui ZL, Hong Q, Li SP. mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis. Nucleic Acids Res. 2006; 34: e71.
20. Yang JJ, Jiang WH, Yang S. mazF as a counter-selectable marker for unmarked genetic modification of Pichia pastoris. FEMSYeast Res. 2009; 9: 600-609.
21. Garcia-Dominguez M, Lopez-Maury L, Florencio FJ, Reyes JC. A gene cluster involved in metal homeostasis in the cyanobacterium Synechocystis sp strain PCC 6803. J Bacteriol. 2000; 182: 1507-1514.
22. Zang XN, Liu B, Liu SM, Arunakumara K, Zhang, XC. Optimum conditions for transformation of Synechocystis sp PCC 6803. J Microbiol. 2007; 45: 241-245.
23. Kaczmarzyk D, Fulda M. Fatty acid activation in cyanobacteria mediated by acyl-acyl carrier protein synthetase enables fatty acid recycling. Plant Physiol. 2010; 152: 1598-1610.